Pan African Journal of Life Sciences最新文献

{"title":"Factors Associated With Intraoperative Conversion to Total Thyroidectomy in Benign Goiters","authors":"S. Olatoke, O. Agodirin, G. Rahman, B. Bolaji, Habeeb Olufemi","doi":"10.36108/pajols/9102/20(0180)","DOIUrl":"https://doi.org/10.36108/pajols/9102/20(0180)","url":null,"abstract":"Background: Decision to undertake total thyroidectomy when gross inspection of the gland raises suspicion of widespread degenerative changes is often intraoperative. Knowing the factors associated with intraoperative conversion to total thyroidectomy may assist preoperative counselling. This study describes the probability of conversion to total thyroidectomy and factors associated with con-version among patients hitherto planned for partial thyroidectomy.\u0000Methods: We reviewed 191 records and extracted data on patient demographics, the pre-operative radiograph findings, the weight of excised gland and the operation performed. Descriptive and inferential statistics were performed. Receiver operator curve was used to assess for cut-off point. P-value was set at 0.05.\u0000Results: A total of 191 records was reviewed consisting of 181 females (94.8% 95% CI 90.6-97.5) and 10 males (5.2%, 95%CI 2.5-9.4). Only nodular goiters required conversion to total thyroidectomy. The over-all probability of total thyroidectomy was 11%(95% CI 7.0-16.3). The probability of total thyroidectomy in female was 10.5%(95% CI 6.4-16.9) while in male was 20%(95% CI2.5-55.6). The probability of total thyroidectomy in a female with nodular goiter was 8.1%(95% CI 4.8-13.5), compared to 28.6%(95% CI 3.7-71) in males. The risk of total thyroidectomy was associated with the weight of the excised gland.\u0000Conclusion: Only nodular goiters required intraoperative conversion to total thyroidecto-my and the probability of conversion was higher in males.","PeriodicalId":234626,"journal":{"name":"Pan African Journal of Life Sciences","volume":"6 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130179441","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of Dietary Inclusion of Sesame indicum Leaves on Haematology and Serum Parameters of Broiler Chickens","authors":"O. S. Adedeji, S. Amao, K. S. Olugbemiga","doi":"10.36108/pajols/9102/20(0130)","DOIUrl":"https://doi.org/10.36108/pajols/9102/20(0130)","url":null,"abstract":"Introduction: Various types of feed additives have been evaluated under commercial conditions and in experimental trials with the aim to achieve improvements on growth performance and the best economic return. Herbs, including Sesame indicum leaves, spices and various plant extracts/essential oils can be used as alternatives to replace antibiotic growth promoters as they are rich in phytochemicals (active compounds) that can be used to stimulate growth and health of the animals. The uses of all these herbs should be studied in relation to the blood and serum indices of the animals in order to determine their harmful level of consuming them by the animals. Therefore, the aim of the study is to determine the effect of different dietary inclusion levels of Sesame indicum leaves on haematology and serum parameters of broiler chickens at both starter and finisher phases.\u0000Methods: A total of one hundred and fifty 1-day old, unsexed (Marshal) broiler chicks were randomly divided into five experimental groups at rate of 30 birds per group. Each group was further subdivided into three replicates at the rate of ten chicks per replicate in a Complete Randomized Design. A maize-soybean basal diet served as control while the Sesame indicum leaves was added to the basal diets at level (0g, 10g, 20g, 30g and 40g/kg) resulting in five formulae, respectively. At the end of the 4th and 8th weeks respectively, six birds were randomly selected from each of the replicate and blood samples were collected from the jugular vein of birds for haematology and serum.\u0000Results: The results indicated that all the haematology and serum parameters were significantly (p < 0.05) affected by the different inclusion levels of S. indicum leaves but the value obtained were within the recommended range while birds fed with the diet containing 40g/kg of S. indicum leaves had the highest values.\u0000Conclusion: Inclusion of S. indicum leaves in the diets appear not to have detrimental effect on the haematology and serum parameters of the experimental birds. The study recommended that 40g/kg inclusion level be adopted as it improved growth performance and general well-being of broiler chickens.","PeriodicalId":234626,"journal":{"name":"Pan African Journal of Life Sciences","volume":"84 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122205220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Low Knockdown Resistance Frequency In Anopheles Gambiae Complex Resistant To Some Who-Approved Insecticides In Selected Areas Of Lagos State, South-West, Nigeria","authors":"T. Idowu, T. Oyeniyi, Tolulope O. Omodara, Olubunmi A. Otunbanjo, Samson T. A wolola","doi":"10.36108/pajols/9102/20(0170)","DOIUrl":"https://doi.org/10.36108/pajols/9102/20(0170)","url":null,"abstract":"Background: Decline in malaria prevalence is usually attributed to the efficient vector control strategies implemented in such endemic areas. The spread of insecticide resistance in Anopheles mosquitoes is a major drawback to the gains in malaria vector control. Here we assessed the susceptibility status of Anopheles gambiae s.l. to some selected WHO-approved insecticides and frequency of knockdown resistance gene in two Local Government Areas of Lagos State, Nigeria.\u0000Methods: Three to five days old adult female mosquitoes were exposed to deltamethrin, permethrin and bendocarb. Knockdown time was recorded every ten minutes and % mortality taken at 24hr post exposure. KDT50 and KDT95 were determined using probit regression analysis. The resistant mosquitoes were used for species identification by Polymerase Chain Reaction (PCR) assays and characterized for the knockdown resistance (kdr) mutation by allele-specific PCR (AS-PCR).\u0000Results: The results of twenty-four hour post exposure mortality rate showed that mosquitoes sampled at differ-ent locations were resistant to permethrin (39% mortality for Lagos Mainland L.G.A.; 45% for Kosofe L.G.A.), del-tamethrin (51% for Lagos Mainland L.G.A; 68% for Kosofe L.G.A), but susceptible to bendiocarb (100% for Lagos Mainland L.G.A.; 99% for Kosofe L.G.A.). The KDT50 varied from 34 minutes in bendiocarb for both LGAs to 190 minutes in deltamethrin for Kosofe and 119 minutes in permethrin for Lagos Mainland. In all insecticides tested for both LGAs, KDT95 was greater than 60 minutes. Only the 1014F kdr mutation was detected. The overall kdr frequen-cy was low (1.79%). There was no significant association between the presence of the 1014F kdr allele and ability of Anopheles mosquitoes to survive exposure to the tested insecticides (P > 0.05).\u0000Conclusion: This level of resistance to WHO approved insecticides is a threat to control programs and can have significant operational impact, if action is not taken.","PeriodicalId":234626,"journal":{"name":"Pan African Journal of Life Sciences","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114062310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chemical and Mineral Compositions of Ostrich (Struthio camelus) egg in Southern Guinea Savanna of Nigeria","authors":"Rasheed Amao","doi":"10.36108/pajols/9102/20(0190)","DOIUrl":"https://doi.org/10.36108/pajols/9102/20(0190)","url":null,"abstract":"Introduction: Egg quality and its components are important indices to consumers, and the economic success of producers depend on the total number of eggs sold. Egg quality is based on the characteristics of the eggs that affect its acceptability, and the egg components are of nutritional importance. Therefore, the study was carried out to determine the chemical and mineral compositions of ostrich eggs found in southern guinea savannah environ of Nigeria.\u0000Methods: Eighty freshly laid eggs were obtained from Ostrich birds in Old Oyo National Park and were used for the experiments. Data were obtained for chemical composition on moisture, carbohydrate, protein, lipid, cholesterol and ash components separately for both albumen and yolk of the eggs while mineral composition observed were Potassium, Calcium, Magnesium, Phosphorus, Iron, copper, Zinc and Manganese.\u0000Results: The findings on chemical composition revealed that the protein (17.35), moisture (85.59) and ash contents (1.90) were significantly (p<0.05) higher in albumen than in yolk. The carbohydrate (0.98), lipid (30.47) and cholesterol (13.40) contents mean percentages were higher in yolk than in albumen. The mineral compositions of albumen and yolk of ostrich eggs indicated that the yolk as a content had the higher values of 135.35, 380.40, 20.35, 10.38, 2.25, 1.85 and 2.50 ppms in calcium, phosphorus, magnesium, iron, copper, zinc and manganese respectively than these variables in albumen while albumen contents was only higher in potassium (160.25 ppm) than in the yolk.\u0000Conclusion: It can be concluded that the information provided in this study could be a template of criteria for comparison and improvement for Ostrich eggs in the environment.","PeriodicalId":234626,"journal":{"name":"Pan African Journal of Life Sciences","volume":"142 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2019-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"134138743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Healthcare associated infections caused by plasmid-encoded blaKPC and blaNDM strains of Klebsiella pneumoniae in Ibadan, Nigeria","authors":"Abigail T. Okunlola, O. Olowe, S. Taiwo","doi":"10.36108/pajols/8102/10(0180)","DOIUrl":"https://doi.org/10.36108/pajols/8102/10(0180)","url":null,"abstract":"Introduction: Carbapenemase producing Klebsiella pneumoniae (CPKP) has recently emerged as major cause of healthcare associated infections (HAIs). These strains are now classified by the US Center for Disease Control as superbugs of urgent concern. This study investigated the occurrence of CPKP in HAIs among hospitalized patients in University College Hospital (UCH), Ibadan.\u0000Methods: Non-duplicate specimens obtained from 250 patients with clinical HAIs were cultured by standard microbiological methods over a period of 6 months. K. pneumoniae was identified using GNB 24E Microbact kit and invitro susceptibility to selected antibiotics was performed by the disk diffusion test. Carbapenemase production was tested by Modified Hodge technique. K pneumoniae carbapenemase (blaKPC) and New Delhi Metallo-β-lactamase (blaNDM) genes were detected by conventional PCR assay. Data were analyzed for the 50 (20%) patients who were culture positive for K pneumoniae infections.\u0000Results: Isolates were resistant to ampicillin (100%), cefuroxime (96%), amoxicillin (92%), gentamicin (86%), nitrofurantoin (80%), ciprofloxacin (80%), ceftazidime (78%) and ofloxacin (72%) but 76% were sensitive to meropenem. No carbapenemase enzyme was detected but 8 of 12 iso-lates resistant to meropenem carried blaKPC while 2 carried both blaKPC and blaNDM genes. The study shows that blaKPC and blaNDM K pneumoniae are involved in clinical infections in Ibadan.\u0000Conclusion: This study reports the second case of K pneumoniae NDM clinical infection in Nigeria. There is need to strengthen the infection control programme of Nigerian hospitals to prevent nationwide spread of these organisms.","PeriodicalId":234626,"journal":{"name":"Pan African Journal of Life Sciences","volume":"601 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116318390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Overview of Molecular Diagnosis in Medical Microbiology","authors":"S. Taiwo","doi":"10.36108/pajols/8102/10(0150)","DOIUrl":"https://doi.org/10.36108/pajols/8102/10(0150)","url":null,"abstract":"Clinical microbiology diagnosis became objectively possible at the end of 19th century with the seminar contributions of researchers such as Louis Pasteur, Robert Koch and other colleagues. The concept of diagnosis then was the ‘culture and isolate’ paradigm, which accelerated to a large extent the growth of bacteriology, as a number of bacteria were able to fulfill the Koch’s postulates, but delayed the growth of other fields of microbiology most especially virology because viruses were not culturable outside of host cells. This paradigm, together with the application of micros-copy, serology and the use of animal models in research in the early 20th century, constituted the ‘first revolution’ in the field of microbiology that is referred to as the conventional microbiological diagnosis. Although this conventional diagnosis has remained valuable, assessments have shown that many of the conventional techniques are not demonstrably ‘fit for purpose’ in the 21st century. This has necessitated the consideration of complementary or alternative technologies, the molecular diagnosis, which has ushered in a ‘second revolution’ in microbiology that is as profound as the first in its impact on our understanding of the microbe-human interactions in health and disease. In this mini-review, an overview of the technologies underlying molecular diagnosis in microbiology is presented. The application of these molecular methods in clinical microbiology laboratory to ensure accurate, reliable and timely release of microbiological test results for better patients’ management and outcome is highlighted.","PeriodicalId":234626,"journal":{"name":"Pan African Journal of Life Sciences","volume":"456 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125798429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Strongyloides stercoralis infection among HIV infected individuals in Osogbo, Osun State, Nigeria","authors":"A. Akindele, S. Adegbola, J. A. Ojo, G. Odewale, O. Opaleye, V. O. Mabayoje, O. Ojurongbe","doi":"10.36108/pajols/8102/10(0110)","DOIUrl":"https://doi.org/10.36108/pajols/8102/10(0110)","url":null,"abstract":"Strongyloides stercoralis is endemic in tropical and subtropical regions and hyper-infection and dissemination could occur in immunocompromised patients. Regular parasitic assessment of HIV patients is needed to prevent disseminated S. stercoralis infection. This study therefore determined the prevalence of S. stercoralis among HIV individuals in Osogbo, Nigeria using stool microscopy and Enzyme-Linked Immunosorbent Assays (ELISA) .Stool and serum samples were collected from HIV individuals who were receiving antiretroviral treatment at Ladoke Akintola University of Technology (LAUTECH) Teaching Hospital Osogbo, Nigeria. Stool samples were examined for S. stercoralis by microscopy. Serology was performed using commercially available ELISA kit to detect parasite-specific immunoglobulin G (IgG).Out of 188 (55 males and 133 females) HIV-infected individuals recruited for the study, 8.5% were positive for S. stercoralis by microscopy while 16.0% were positive by ELISA and 39 (20.7%) HIV individuals were positive with any of the two methods. The highest detection rate (73.1%) was observed with ELISA while microscopy recorded a detection rate of 39%. HIV individuals with CD4 ≤200 had a significantly higher prevalence (29.5%) compared with those with CD4 ≥200 (22.4%) (p = 0.0064).The findings of this study show that the combination of both ELISA and microscopy may improve the problem associated with the diagnosis of S. stercoralis in Nigeria.","PeriodicalId":234626,"journal":{"name":"Pan African Journal of Life Sciences","volume":"15 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128688771","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biochemical Properties of Rhodanese from Almond (Prunus amygdalus) Nuts","authors":"A. Ehigie, M. Abdulrasak, O. L. Ehigie","doi":"10.36108/pajols/8102/10(0140)","DOIUrl":"https://doi.org/10.36108/pajols/8102/10(0140)","url":null,"abstract":"Study of the characteristic pattern of enzymes are useful in the understanding of certain physiological and biochemical process-es. Thiosulfate: cyanide sulfurtransferase (rhodanese) is a ubiquitous multifunctional enzyme, that is believed to function in cyanide detoxification. The present study was conducted to determine the activity of rhodanese in almonds (Prunus amygdalus) that belong to the rose family, rosaceae. Rhodanese from the almond nuts was purified by ammonium sulphate precipitation, ion exchange and affinity chromatography. The molecular weight of the enzyme was determined by sodium dodecyl sulphate poly-acrylamide gel electrophoresis. The purified rhodanese from the almond nuts had a specific activity of 5.09 RU/mg with yield of 0.06%. A Km value of 11.14 mM with Vmax 0.46 RU/ml/min were obtained from KCN while a Km value of 13.95 mM with Vmax of 0.48 RU/ml/min was obtained from Na2S2O3. The substrate specificity studied indicated that Mercapto-ethanol (MCPE), Ammonium per sulfate ((NH2)2S2O8, Ammonium sulfate ((NH2)2SO4, Sodium sulfate (Na2SO4) and Sodium metabi-sulfate (Na2S2O5) cannot be substituted for sodium thiosulphate (Na2S2O3) as sulphur donor for rhodanese catalytic reaction. The optimum activity of the enzyme was observed at 50oC and an optimum pH of 8. The effect of metals on rhodanese from Almond nut showed that at 1 mM concentration of the metals used did not pronouncedly affect the activity of the enzyme metals except that of HgCl2 and MnCl2. However, the divalent metals including MnCl2 HgCl2, CaCl2, and BaCl2 inhibited the enzyme at 10 mM concentration. The molecular weight obtained from sodium dodecyl sulphate polyacrylamide gel electrophoresis was estimated to be 35 kDa. The study validates the expression of rhodanese activity in almond nut. The characteristic property of rhodanese in the plant may be exploited for bioremediation of cyanide polluted soil.","PeriodicalId":234626,"journal":{"name":"Pan African Journal of Life Sciences","volume":"36 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"128125660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Interleukin -10 – 1082A/G single nucleotide polymorphism and risk of Type 2 Diabetes Mellitus in a Southwestern population of Nigeria","authors":"O. G. Ayelagbe, O. Daodu, O. Akanbi, A. Onuegbu, O. Ojurongbe","doi":"10.36108/pajols/8102/10(0190)","DOIUrl":"https://doi.org/10.36108/pajols/8102/10(0190)","url":null,"abstract":"Introduction: Type 2 diabetes mellitus (T2DM) is a metabolic, multifactorial disease caused by genetic, dietary and environmental interactions with alterations in the pattern of cytokine expression. Association between interleukin (IL)-10 polymorphism and T2DM is not known in Black Af-ricans. This study therefore investigates the relationship between IL-10 -1082A/G polymorphism and risk of T2DM in Southwest Nigeria.\u0000Methods: 100 patients with T2DM and 105 healthy controls were enrolled. Anthropomet-ric indices were measured and fasting blood sample was obtained from all subjects. Plasma glucose and lipid profile were estimated by standard laboratory procedures. IL-10 (-1082A/G) gene polymorphism was genotyped using Allele Refractory Mutation detection System-Polymerase Chain Reaction (ARMS-PCR) technique. Odds ratio analysis (95%CI) was used to determine association between genotype and diabetes mellitus in studied subjects.\u0000Results: T2DM patients had the highest frequency of IL 10 -1082G/G genotype (58.3%) while -1082AG frequency was highest (63.1%) in non-diabetics. Non- significant associations between IL 10 -1082 gene polymorphism and T2DM risk (for AG genotype: OR=0.95, 95%CI =0.19-4.2, p = 0.95; for GG genotype: OR=0.49, 95%CI = 0.10-2.41, p = 0.38) were recorded. Fasting plasma glucose, 2hours post-prandial, total cholesterol and triglyceride were all significantly elevated in IL 10 -1082G/G than AG and AA genotypes in T2DM subjects (p<0.001). Expression of A allele was higher in controls than DM patients .\u0000Conclusion: This case-control study did not find a significant association between IL 10-1082A/G polymorphism and T2DM; however further studies with larger sample size and IL-10 plasma level meas-urement will be recommended to validate this observation.","PeriodicalId":234626,"journal":{"name":"Pan African Journal of Life Sciences","volume":"5 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"117157203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Usefulness of Polymerase Chain Reaction in the diagnosis of asymptomatic malaria among school age children in Ilorin, Nigeria","authors":"Ayodele Adedoja, S. K. Babatunde, B. D. Tijani, A. Akanbi, O. Ojurongbe","doi":"10.36108/pajols/8102/10(0170)","DOIUrl":"https://doi.org/10.36108/pajols/8102/10(0170)","url":null,"abstract":"Introduction: In Nigeria, microscopy and Rapid Diagnostic Tests (RDTs) are majorly used routinely to diagnose malaria in clinical settings. In recent years, molecular diagnosis has emerged as the most sensitive method for malaria diagnosis. This study determined the usefulness of Polymerase Chain Reaction (PCR) in comparison to microscopy (Giemsa stained thick and thin smears) for the diagnosis of asymptomatic malaria in Ilorin, Nigeria.\u0000Methods: Th e study enrolled 310 school children aged 4–15 years with no sign or symptoms of malaria. Blood samples were collected for identification of Plasmodium species infection using light microscopy and conventional PCR.\u0000Results: The PCR method detected m o r e infection of P. falciparum 107 (34.5%) than slide microscopy 81 (26.1%) in the study. Only P. falciparum was detected by microscopy while PCR detected mono infection of P. malariae (3.2%) and P. ovale (0.6%) and mixed infection of P. falciparum and P. malariae (3.2%). Overall the PCR method detected more malaria parasite compared to microscopy.\u0000Conclusion: The PCR technique, although more laborious and expensive than microscopy, have better diagnostic accuracy and are highly useful for the detection of P. falciparum and other malaria species in asymptomatic and low parasitaemia cases.","PeriodicalId":234626,"journal":{"name":"Pan African Journal of Life Sciences","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2018-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"114118279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}