Turkish Journal of Biochemistry-turk Biyokimya Dergisi最新文献_第7页

Major fatty acids composition of 32 almond (Prunus dulcis (Mill.) D.A. Webb) genotypes distributed in East and Southeast of Anatolia 32种杏仁(Prunus dulcis)的主要脂肪酸组成d.a b Webb)基因型分布在安纳托利亚东部和东南部

IF 0.7 4区生物学

Turkish Journal of Biochemistry-turk Biyokimya Dergisi Pub Date : 2014-01-01 DOI: 10.5505/TJB.2014.55477

H. Karatay, A. Aslan

{"title":"Major fatty acids composition of 32 almond (Prunus dulcis (Mill.) D.A. Webb) genotypes distributed in East and Southeast of Anatolia","authors":"H. Karatay, A. Aslan","doi":"10.5505/TJB.2014.55477","DOIUrl":"https://doi.org/10.5505/TJB.2014.55477","url":null,"abstract":"Objective: In this study, the fatty acid compositions of 32 different almond (Prunus dulcis (Mill.) D.A. Webb) genotypes seeds that collected from South and South East Anatolia regions in Turkey were studied. Methods: For lipid extraction of almond genotypes Hara and Radin (1978) method were used. Fatty acids content were determined using gas chromatographic (GC) analysis. The datas were evalulated with SPSS 17.0 statistical program. Results: In the gas chromatographic analysis, palmitic acid (16:0), palmitoleic acid (16:1), stearic acid (18:0), oleic acid (18:1), linoleic acid (18:2), and linolenic acid (18:3) were determined to be 5.34%, 0.70%, 0.85%, 74.46%, 17.89%, and 0.75% respectively. Saturated fatty acids (SFA) 6.19%, unsaturated fatty acids (USFA) 93.81% and a rate of USFA/SFA of 15.40, monounsaturated fatty acids (MUFA) 75.16%, polyunsaturated fatty acids (PUFA) 18.65% and a MUFA/PUFA ratio of 4.32 were found. In the correlation analysis, the highest correlation coefficient (r=-0.988) was detected between oleic acid and linoleic acid. Relationships among almond samples were partially identified with the cluster analysis. Conclusion: In this study, the variations were found between almond genotypes collected from different locations in terms of fatty acids compositions. Besides, the almond genotypes that have high quality unsaturates fatty acids such as high oleic acids and low linoleik acids content","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"14 1","pages":"307-316"},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84917798","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18

The diagnostic value of SCUBE1 levels in acute ischemic stroke SCUBE1水平对急性缺血性脑卒中的诊断价值

IF 0.7 4区生物学

Turkish Journal of Biochemistry-turk Biyokimya Dergisi Pub Date : 2014-01-01 DOI: 10.5505/TJB.2014.43534

M. Gunaydin, S. Turkmen, A. Şahin, A. Sumer, A. Menteşe, S. Turedi, A. Gunduz, S. Karahan

{"title":"The diagnostic value of SCUBE1 levels in acute ischemic stroke","authors":"M. Gunaydin, S. Turkmen, A. Şahin, A. Sumer, A. Menteşe, S. Turedi, A. Gunduz, S. Karahan","doi":"10.5505/TJB.2014.43534","DOIUrl":"https://doi.org/10.5505/TJB.2014.43534","url":null,"abstract":"Aim: Stroke is the third most important cause of death after coronary artery disease and cancer, and the most important among those diseases leading to disability. Recent studies have shown that early diagnosis and treatment of patients presenting to the emergency department with stroke can reduce the effect of the disease on mortality and morbidity. The purpose of this study was to determine the diagnostic value of plasma SCUBE1, a novel biochemical marker thought to be capable of use in ischemic conditions, values in the diagnosis of acute ischemic stroke in the emergency department. Materials and Methods: Thirty patients diagnosed with acute ischemic stroke at the Karadeniz Technical University Faculty of Medicine Emergency Department, Turkey, between May and October, 2011, and a control group of 30 healthy volunteers were included. An enzyme-linked immunosorbent assay kit was used to determine SCUBE-1 levels. Patient and control group plasma SCUBE1 values were compared. Results: Mean age in the patient group was 74.50 ± 10.50, and 59.93 ± 12.63 in the control group. Mean 6th hour SCUBE1 value in the patient group was 25.104 ± 15.837 ng/ml, and the mean 12th hour SCUBE1 value was 27.395 ± 14.146 ng/ml. Mean control group SCUBE1 value was 35.019 ± 22.310 ng/ml. Control group SCUBE1 values were higher than those of the patient group. Sixth hour SCUBE value was statistically significant when the patient and control groups were compared with age-adjusted values (p = 0.626). No statistically significant difference was determined between 6th and 12th hour SCUBE1 values (p = 0.334). Conclusion Plasma SCUBE1 values in acute ischemic stroke patients did not rise at significant levels compared to the control group, and are therefore not useful in the early diagnosis of acute ischemic stroke.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"3 1","pages":"107-112"},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78968539","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 6

Production of an extracellular lipase by Candida utilis NRRL-Y-900 using agro-industrial by-products 念珠菌利用农工副产品NRRL-Y-900生产胞外脂肪酶

IF 0.7 4区生物学

Turkish Journal of Biochemistry-turk Biyokimya Dergisi Pub Date : 2014-01-01 DOI: 10.5505/TJB.2014.96977

A. Rehman, Sunniya Rasool, H. Mukhtar, I. Haq

{"title":"Production of an extracellular lipase by Candida utilis NRRL-Y-900 using agro-industrial by-products","authors":"A. Rehman, Sunniya Rasool, H. Mukhtar, I. Haq","doi":"10.5505/TJB.2014.96977","DOIUrl":"https://doi.org/10.5505/TJB.2014.96977","url":null,"abstract":"Aim:To screen various yeast cultures and agro-industrial by-products and optimization of fermentation conditions for the microbial production of extracellular lipase under solid state fermentation technique. Material and Methods: Various yeast cultures including Candida lipolytica NRRL-Y-1095, Candida utilis NRRL-Y-900, Candida tropicalis NRRL-Y-1552, Saccharomyces cerevisiae IIB-1 were screened by culturing on agro-industrial by-products under batch culture using solid state fermentation in 250 mL Erlenmeyer flasks. The medium was supplemented with various nitrogen sources (both organic and inorganic) and metal ions. Results: Candida utilis NRRL-Y-900 showed the highest enzyme production on soybean meal. Various particle sizes of substrate and moistening agents were also optimized for the maximum lipase synthesis. The optimum temperature and pH for the accumulation of enz- yme by Candida utilis NRRL-Y-900 was 30oC and 6.5, respectively. The fermentation time of 60h was suitable for the maximum enzyme production by using 7.5% inoculum of 24h old yeast culture. The optimal medium composition consisted of 2% (w/v) meat extract, 0.4% (w/v) ammonium sulphate and 5mM Fe+2. The maximum extracellular lipase production was 3.96±0.09 U. Conclusion: The results obtained during the study are significant for, to our knowledge, it is the first report regarding the utilization of soybean meal by C. utilis NRRL-Y-900 to accu- mulate lipase under optimized conditions and solid state fermentation.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"8 1","pages":"140-149"},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88248277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

An assessment of phytochemical constituents and antioxidant potential of Delphinium malabaricum (Huth) Munz malabaricum (Huth) Munz的植物化学成分和抗氧化潜力的评价

IF 0.7 4区生物学

Turkish Journal of Biochemistry-turk Biyokimya Dergisi Pub Date : 2014-01-01 DOI: 10.5505/TJB.2014.47965

R. Firdose, R. Swaroopa, V. Vinayak, B. D. Ghansham

{"title":"An assessment of phytochemical constituents and antioxidant potential of Delphinium malabaricum (Huth) Munz","authors":"R. Firdose, R. Swaroopa, V. Vinayak, B. D. Ghansham","doi":"10.5505/TJB.2014.47965","DOIUrl":"https://doi.org/10.5505/TJB.2014.47965","url":null,"abstract":"Objective: The aim of the study was to examine the efficiency of different extraction methods for the determination of total phenolics, flavonoids and alkaloid contents contributing to antioxidant capacity of Delphinium malabaricum. Methods: The extracts of different plant parts (roots, stems and leaves) of Delphinium malabaricum were prepared in aqueous and various organic solvents and the extracts were evaluated for phenolics, flavonoids and alkaloid contents as the equivalents of gallic acid, rutin, and colchicine; respectively. The antioxidant capacity of the extracts was also assessed by 1,1-diphenyl-2-picrylhydrazyl free radical scavenging activity and ferric reducing antioxidant power assays in both fresh and dry plant tissues and the difference in fresh and dry extracts on phytochemical constituents and antioxidant activities were compared. Results: The aqueous extracts of roots exhibited the highest total phenolic (4.94 mg gallic acid/g fresh weight, 13.4 mg gallic acid/g dry weight) and total alkaloid content (8.05 mg colchicine/g fresh weight, 20.4 mg colchicine/g dry weight) as compared to stem, leaves and other solvent extracts. Whereas, flavonoid contents were found to be highest in the leaf extracts (5.36 mg rutin/g fresh weight, 7.88 mg rutin/g dry weight). Interestingly the aqueous extracts of all the plant parts exhibited highest yield of phenolic, flavonoids and alkaloids as compare to the other solvents used for the extraction. Antioxidant activity assays exhibited considerable antioxidant potential and showed expected significant positive correlation with the phytochemical compounds. Conclusion: The study specified that aqueous extracts are more effective to extract phenols, flavonoids, alkaloids and antioxidants from Delphinium malabaricum than organic extracts and roots have higher level and the alkaloids were found to be higher comparing to that of phenolics and flavonoids content per gram dry weight of plant tissue.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"53 5","pages":"277-284"},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72559547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Effect of econazole on Ca2+ signaling in human colorectal adenocarcinoma cells 益康唑对人结直肠癌细胞Ca2+信号传导的影响

IF 0.7 4区生物学

Turkish Journal of Biochemistry-turk Biyokimya Dergisi Pub Date : 2013-06-01 DOI: 10.5505/TJB.2013.08370

Celia Carrillo, M. M. Cavia, S. Alonso-Torre

引用次数: 1

Structural stability of commercial ficin under different denaturing conditions 商品纤维在不同变性条件下的结构稳定性

IF 0.7 4区生物学

Turkish Journal of Biochemistry-turk Biyokimya Dergisi Pub Date : 2013-04-01 DOI: 10.5505/TJB.2013.33043

Nabil Abdul, Aleem Sidek, Adyani Azizah, A. Halim, H. Kadir, Saad Tayyab

引用次数: 2

Is procalcitonin a more sensitive parameter than other acute phase reactants for early infection in arthroplasty 降钙素原是关节置换术早期感染的一个比其他急性期反应物更敏感的参数吗

IF 0.7 4区生物学

Turkish Journal of Biochemistry-turk Biyokimya Dergisi Pub Date : 2013-01-01 DOI: 10.5505/TJB.2013.83584

A. Özmeriç, H. Burç, Nevres Hürriyet, Y. Baykal, T. Atay

{"title":"Is procalcitonin a more sensitive parameter than other acute phase reactants for early infection in arthroplasty","authors":"A. Özmeriç, H. Burç, Nevres Hürriyet, Y. Baykal, T. Atay","doi":"10.5505/TJB.2013.83584","DOIUrl":"https://doi.org/10.5505/TJB.2013.83584","url":null,"abstract":"Objectives: The routine laboratory parameters for detection of early infection could be increased after surgical trauma in endoprosthesis surgery. The aim of this study was to compare the early infective complication marker Procalcitonin with routine markers. Methods: Twenty patients with primary total hip prosthesis and 30 knee prosthesis were enrolled. The changes in procalcitonin, C-reactive protein levels, white blood cell count, and erythrocyte sedimentation rate were evaluated preoperatively, at postoperative first day, postoperative fifth day and on the day of discharge. Results: Procalcitonin values of patients who developed superficial infection were statistically high in comparison with uncomplicated patients at post-op Day 1 and Day 5 (p < 0.05). The level of C-reactive protein, white blood cell count, and erythrocyte sedimentation rate peaked on postoperative Day 1. These levels decreased by postoperative Day 5 and on the day of discharge but did not reach preoperative mean values. Conclusion: Procalcitonin is a more selective parameter to predict early infection status following total endoprosthetic surgery. When factors that cause an inflammatory response were eradicated, procalcitonin levels dropped more rapidly and followed a standard postoperative kinetic pathway.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"27 1","pages":"337-344"},"PeriodicalIF":0.7,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73885484","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differentiation of hematopoietic stem cells isolated from peripheral blood to megakaryocyte 外周血分离的造血干细胞向巨核细胞的分化

IF 0.7 4区生物学

Turkish Journal of Biochemistry-turk Biyokimya Dergisi Pub Date : 2013-01-01 DOI: 10.5505/TJB.2013.52714

O. Ozakpinar, A. Maurer, C. Adiguzel, O. T. Cilingir, M. Demi̇r, F. Uras

{"title":"Differentiation of hematopoietic stem cells isolated from peripheral blood to megakaryocyte","authors":"O. Ozakpinar, A. Maurer, C. Adiguzel, O. T. Cilingir, M. Demi̇r, F. Uras","doi":"10.5505/TJB.2013.52714","DOIUrl":"https://doi.org/10.5505/TJB.2013.52714","url":null,"abstract":"Objective: Thrombocytopenia remains a serious problem in patients treated with highdose chemotherapy and bone marrow transplantation. In recent years, infusion of ex vivo expanded megakaryocytes (Mk) progenitors into patients has been proposed as a strategy for shortening the time of platelet engraftment. The development of in vitro culture methods to obtain sufficient numbers of Mks from haematopoietic stem cells (HSC) is an important target in basic and clinical research projects. The aim of this study was to develop a two-step ex vivo expansion culture system of Mk progenitors from peripheral blood stem cells (PBSC). Methods: PBSC were harvested from three healthy adult donors. CD34+ cells were isolated and cultured in serum free media supplemented with thrombopoietin (TPO) (50 ng/ml), Interleukin 3 (IL-3) (20 ng/ml) and Interleukin 6 (IL-6) (20 ng/ml) for 12 days followed by an incubation with IL-6 (20 ng/ml) and TPO (50 ng/ml) for another 9 days. The differentiation of Mks was monitored by flow cytometry (% of CD34+/41+ cells). The morphology of the cells was studied by light, electron and fluorescence microscopy. Results: Morphological analysis of cells generated after 7 days of culture showed typical aspects of developing Mks. The percentage of CD41+ cells was higher than 70 on day 21. Conclusion: The results obtained in this study demonstrated that this two-step culture system is an effective method to obtain high rates of megakaryocytes. It is obvious that this promising method needs further development for clinical applications.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"11 1","pages":"243-249"},"PeriodicalIF":0.7,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.5505/TJB.2013.52714","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72508886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Relationship Between Glycemic Control And Asymmetrical Dimethylarginine Levels 血糖控制与不对称二甲基精氨酸水平的关系

IF 0.7 4区生物学

Turkish Journal of Biochemistry-turk Biyokimya Dergisi Pub Date : 2013-01-01 DOI: 10.5505/TJB.2013.43153

O. Ç. Madenci, N. Yucel, Z. Arıkan, M. Sargin, Derya Akbaba, O. Kaya, A. Kaptanagasi

引用次数: 2

The Effects of Topoisomerase Inhibition on Dna Repair and Apoptosis in L929 Fibroblasts 拓扑异构酶抑制对L929成纤维细胞Dna修复和细胞凋亡的影响

IF 0.7 4区生物学

Turkish Journal of Biochemistry-turk Biyokimya Dergisi Pub Date : 2013-01-01 DOI: 10.5505/TJB.2013.32032

Irem Dogan, A. S. Yar, Volkan Ergin, S. Menevşe, A. Menevşe, A. Ekmekçi

{"title":"The Effects of Topoisomerase Inhibition on Dna Repair and Apoptosis in L929 Fibroblasts","authors":"Irem Dogan, A. S. Yar, Volkan Ergin, S. Menevşe, A. Menevşe, A. Ekmekçi","doi":"10.5505/TJB.2013.32032","DOIUrl":"https://doi.org/10.5505/TJB.2013.32032","url":null,"abstract":"Objective: DNA repair pathways in cells are essential for the maintenance of genome integrity, and for countering the induction of tumorigenesis. Topoisomerase II is a nuclear enzyme that functions during both DNA replication and transcription. The topoisomerase II inhibitor etoposide is an antineoplastic drug that has been used to generate DNA damage and maintain apoptosis. Etoposide blocks cell division by interfering with the topoisomerase II and generates double strand breaks. Application of topoisomerase II inhibitors leads to the formation of double strand breaks that are rapidly repaired following removal of the drug. In the present study, we searched the apoptotic events and early double strand DNA repair process that prevent the apoptotic cell death of L929 fibroblasts in response to treatment with etoposide. Methods: Cytotoxicty of etoposide on L929 cells was determined in a time and dose dependent manner with MTT assay. The double strand DNA breaks were determined with comet assay. Acridin orange/Ethidium bromide fluorescence staining and Caspase 3/7 activity assays were performed in determined etoposide concentrations at 24 hour. Quantitative mRNA expressions of DNA repair genes (Ku70, Ku80, BRCA2, Rad51, XRCC4) were determined after etoposide treatment. Results: The levels of apoptotic cell markers and DNA double strand breaks were elevated in the increasing doses and time. The relative expression levels of Rad51, XRCC4 and BRCA2 were unstable in a time and dose dependent manner. Ku80 levels were generally decreased in etoposide treated groups when compared with controls. However, Ku70 was highly expressed at 9 and 12 hour with the increasing doses. Conclusion: According to the results of this study, etoposide activates apoptotic events. Also, low expression levels of DNA repair enzymes prevent cell survival from DNA damage.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"11 1","pages":"229-237"},"PeriodicalIF":0.7,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81999784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0