Turkish Journal of Biochemistry-turk Biyokimya Dergisi最新文献_第8页

Changes in Gene Methylation Following Chemotherapy in Breast Cancer Cell Lines 乳腺癌细胞系化疗后基因甲基化的变化

IF 0.7 4区生物学

Turkish Journal of Biochemistry-turk Biyokimya Dergisi Pub Date : 2013-01-01 DOI: 10.5505/TJB.2013.46320

F. Ari, R. Napieralski, E. Ulukaya

{"title":"Changes in Gene Methylation Following Chemotherapy in Breast Cancer Cell Lines","authors":"F. Ari, R. Napieralski, E. Ulukaya","doi":"10.5505/TJB.2013.46320","DOIUrl":"https://doi.org/10.5505/TJB.2013.46320","url":null,"abstract":"Objective: Epigenetic modulation of gene expression by DNA promoter methylation may contribute to acquired resistance to chemotherapy in cancer cells. Decitabine (5-aza-2'- deoxycytidine), a demethylating agent, may act synergistically with standard chemotherapy regimens to activate epigenetically silenced genes. In the present in vitro study, it was investigated the effect of gene methylation level after treatment with decitabine and combination of decitabine with anthracycline-based therapeutics (5-fluorouracil plus epirubicine plus cyclophosphamide; FEC) on breast cancer cells (MCF-7 and MDA-MB-231). Methods: The effect of decitabine and its combination with FEC on different genes methylation level has been tested in MDA-MB-231 and MCF-7 human breast cancer cell lines. The effect of decitabine on the cell viability was assayed by MTT assay. Methylight real-time PCR and methylation specific PCR were carried out to determine the methylation status of certain genes: DAPK, TMS1, MGMT and the global methylation marker LINE-1. Results: The LINE-1 methylation status significantly decreased in both cell lines after treatment with the combination of decitabine with FEC. In MDA-MB-231 cells, methylation of the TMS1 and the MGMT gene promoter was significantly reduced by FEC plus decitabine while no effect was observed in MCF-7 cells. Conclusion: Anthracycline-based therapy regimens in combination with demethylating agents such as decitabine may affect chemotherapy outcome by modulation of apoptosis- relevant genes by methylation. More importantly, this modulation seems to be dependent on","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"152 1","pages":"154-162"},"PeriodicalIF":0.7,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73990593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

An Experimental Design Technique: Randomized Block Design 一种实验设计技术:随机区组设计

IF 0.7 4区生物学

Turkish Journal of Biochemistry-turk Biyokimya Dergisi Pub Date : 2013-01-01 DOI: 10.5505/TJB.2013.04796

E. Karaağaoğlu

引用次数: 2

Improved Performance of Pseudomonas fluorescens lipase by covalent immobilization onto Amberzyme 共价固定化荧光假单胞菌脂肪酶的研究

IF 0.7 4区生物学

Turkish Journal of Biochemistry-turk Biyokimya Dergisi Pub Date : 2013-01-01 DOI: 10.5505/TJB.2013.30085

Y. Aslan, N. Handayani, Erythrina Stavila, K. Loos

{"title":"Improved Performance of Pseudomonas fluorescens lipase by covalent immobilization onto Amberzyme","authors":"Y. Aslan, N. Handayani, Erythrina Stavila, K. Loos","doi":"10.5505/TJB.2013.30085","DOIUrl":"https://doi.org/10.5505/TJB.2013.30085","url":null,"abstract":"Objective: In this study, the conditions of covalent immobilization of Pseudomonas fluorescens lipase onto an oxirane-activated support (Amberzyme) were optimized to obtain a high activity yield. Furthermore, the operational and storage stabilities of immobilized lipase were tested. Methods: Optimum conditions for immobilization were determined by changing individually the conditions (pH from 5 to 9; buffer concentration from 0.025 to 2.5 M; amount of Amberzyme from 100 to 500 mg and duration of immobilization from 24 to 120 h). Amounts of protein and the activity of enzyme were determined by UV/Vis (PYE UNICAM SP8-200 UV/Vis spectrophotometer). Results: Immobilization conditions (pH and molar concentration of immobilization buffer, enzyme/support ratio and immobilization duration) significantly affected the immobilization efficiency. 100% immobilization yield and 145% activity yield were achieved by optimizing the immobilization conditions. Operational and storage stabilities of immobilized lipase were determined as well. The immobilized enzymes retained its activity for 20 consecutive batch reactions. Furthermore, the immobilized lipase showed a high storage stability as no decrease in its activity was observed for 20 days. Conclusion: Our results obtained in the present study are the best in the covalent immobilization of Pseudomonas fluorescens lipase in the literature. Therefore our future studies will focus on using the immobilized Pseudomonas fluorescens lipase for the production of biodiesel, hydrolysis of oils and various important esterification reactions.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"143 1","pages":"313-318"},"PeriodicalIF":0.7,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84197355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

In vitro antioxidant and antibacterial properties and total phenolic contents of essential oils from Thymus vulgaris, T. kotschyanus, Ziziphora tenuior and Z. clinopodioides 研究了百里草、山茱萸、青花草和山茱萸精油的体外抗氧化、抗菌性能及总酚含量

IF 0.7 4区生物学

Turkish Journal of Biochemistry-turk Biyokimya Dergisi Pub Date : 2013-01-01 DOI: 10.5505/TJB.2013.58070

J. Aliakbarlu, Farnaz Shameli

引用次数: 34

The Multidisciplinary Approach to Biochemistry Laboratory Education 生物化学实验教学的多学科方法

IF 0.7 4区生物学

Turkish Journal of Biochemistry-turk Biyokimya Dergisi Pub Date : 2013-01-01 DOI: 10.5505/TJB.2013.02360

Y. Ozkan, F. Erkoç, H. Çelik, A. Dinçel, B. Şimşek, L. Kayrın, Subhan Ekşioğlu, M. Yüksel, G. Haklar, Özlem Yavuz, S. Kurban, H. Uysal, U. Kisa, M. Konuk, E. Bodur, M. Selvi, G. Akça

引用次数: 2

Heteroplasmy and length variation in the tRNApro- Dloop regions of three sturgeon species (A. stellatus, A. gueldenstaedtii and H. huso) from the Turkish coast of the Black Sea 黑海土耳其海岸三种鲟鱼(A. stellatus, A. gueldenstaedtii和H. huso) tRNApro- Dloop区域的异质性和长度变化

IF 0.7 4区生物学

Turkish Journal of Biochemistry-turk Biyokimya Dergisi Pub Date : 2013-01-01 DOI: 10.5505/TJB.2013.70783

Y. Çi̇ftçi, O. Eroğlu, Ş. Firidin

{"title":"Heteroplasmy and length variation in the tRNApro- Dloop regions of three sturgeon species (A. stellatus, A. gueldenstaedtii and H. huso) from the Turkish coast of the Black Sea","authors":"Y. Çi̇ftçi, O. Eroğlu, Ş. Firidin","doi":"10.5505/TJB.2013.70783","DOIUrl":"https://doi.org/10.5505/TJB.2013.70783","url":null,"abstract":"Objective: To determine tandem repeat polymorphism and heteroplasmy in three sturgeon species (A. stellatus, A. gueldenstaedtii and H. huso) from the Turkish coast of Black Sea. Methods: tRNApro and D-loop segment of mtDNA from three sturgeon species were amplified via PCR and sequenced. For each species, homoplasmic individuals with different product lengths, repeat motifs and regions were determined and the repeat numbers and frequencies were calculated. The variation in mtDNA size present in the overall sample of sturgeon from Turkish waters was apportioned into hierarchical components. Also, the statistical approach described by other researchers was used for the calculation of inter- species and intra-species genetic variation. Results: The results showed that all three species reveal 2-6 copies of different mtDNA length variants attributable to varying copy numbers of an 82-84bp repeat sequences. A total of 9.9% of the sturgeons were heteroplasmic, bearing three to five repeat variants. The highest number of observed repeat units rate was 45.8% in 3 repeats morph in A. gueldenstaedtii. The mean genetic diversity within individuals (Kb) was higher in A. gueldenstaedtii and A. stellatus than in H. huso (0.625, 0.620, and 0.500, respectively). Conclusion: The repeat region, responsible for length variations and heteroplasmy, is located near the end of the D-loop and control region separated by only a few nucleotides from the tRNApro gene.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"9 1","pages":"250-257"},"PeriodicalIF":0.7,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88916482","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Medicine in philately, Karl Landsteiner: the father of blood grouping 医学上的集邮，卡尔·兰德斯坦纳:血型分类之父

IF 0.7 4区生物学

Turkish Journal of Biochemistry-turk Biyokimya Dergisi Pub Date : 2013-01-01 DOI: 10.5505/TJB.2013.73644

E. E. V. Lutz, A. Ataman

引用次数: 0

Optimization of dilute acid and alkaline peroxide pretreatment to enhance bioethanol production from wheat straw by co-fermentation 稀酸和碱性过氧化预处理提高麦秸共发酵生物乙醇产量的优化

IF 0.7 4区生物学

Turkish Journal of Biochemistry-turk Biyokimya Dergisi Pub Date : 2013-01-01 DOI: 10.5505/TJB.2013.57431

Pınar Karagöz, M. Özkan

引用次数: 2

Purification and characterization of a pH and heat stable esterase from Geobacillus sp. TF17 Geobacillus sp. TF17中pH和热稳定酯酶的纯化及特性研究

IF 0.7 4区生物学

Turkish Journal of Biochemistry-turk Biyokimya Dergisi Pub Date : 2013-01-01 DOI: 10.5505/TJB.2013.36035

A. Colak

{"title":"Purification and characterization of a pH and heat stable esterase from Geobacillus sp. TF17","authors":"A. Colak","doi":"10.5505/TJB.2013.36035","DOIUrl":"https://doi.org/10.5505/TJB.2013.36035","url":null,"abstract":"Objective: To purify and characterize an esterase from a thermophilic bacterium, Geobacillus sp. TF17. Methods: The crude esterase was purified by using acetone precipitation and ion exchange choromatography methods and characterized. Results: The optimum temperature and pH of the enzyme were found to be 50°C and 7.5, respectively. The purified enzyme was extremely stable in the range of pH 4.0-9.0 after 72 hour incubation at 4°C. The thermal stability profile shows that this enzyme is stable in the range of 30-50°C after 72 h incubation. The Km and Vmax values for this esterase in the presence of p-nitrophenyl butyrate (pNPB) as substrate were found as 0.056 mM and 19.38 U/mg protein, respectively. The enzyme activity was inhibited more than about 60% in the presence of some organic solvents such as isopropanol and acetonitrile. Additionally, it was detected that some metal ions affect the enzyme activity at different ratio. Conclusion: An esterase was purified and characterized from Geobacillus sp. TF17. The pH and thermal stability of purified enzyme are quite high. The data obtained from this study show that the purified esterase has advantages for industrial or biotechnological applications in terms of especially its high thermal- and pH-stability.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"212 1","pages":"329-336"},"PeriodicalIF":0.7,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77066596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

The relationship of antioxidants with aging 抗氧化剂与衰老的关系

IF 0.7 4区生物学

Turkish Journal of Biochemistry-turk Biyokimya Dergisi Pub Date : 2013-01-01 DOI: 10.5505/TJB.2013.21939

I. Geyikli, M. Akan, M. Tarakçıoğlu

{"title":"The relationship of antioxidants with aging","authors":"I. Geyikli, M. Akan, M. Tarakçıoğlu","doi":"10.5505/TJB.2013.21939","DOIUrl":"https://doi.org/10.5505/TJB.2013.21939","url":null,"abstract":"Objective: In this study, it was aimed to investigate the changing levels of antioxidants along with aging. Malondialdehyde (MDA), total antioxidant (TAS) levels, and paraoxanase (PON) and arylesterase enzyme activities were examined as well as Coenzyme Q10, that has an immense role in production of ATP and, in fact, is a potent antioxidant with a lipophillic structure Methods: Research included 160 men and women whose ages differ from 20 to 70 years old and also live in Gaziantep area. Research was done by dividing subjects into 3 age groups: 50 people belonged to 20–30, 75 people to 31–50 and 35 people to 51–70 age group. Groups were constituted from people who are non-obese and who do not have any physical complaints. In taken plasma samples coenzyme Q10, MDA and TAS levels, PON and arylesterase enzyme activities were measured. The measurements were performed as follows: MDA by using thiobarbituric acid method, TAS, PON and arylesterase with spectrophotometric methods, and coenzyme Q10 with HPLC method were measured. Results: A correlation was found between age and coenzyme Q10 in our study (p<0.001). Coenzyme Q10 was found to be increased with aging. Statistically significant and negative linear relationship was found between coenzyme Q10, MDA levels, and PON and arylesterase activities (p<0.01, p<0.001). Conclusion: Despite the aging, the antioxidant system in healthy people works to protect the body from the endogenous or exogenous oxidants. However, levels of antioxidants cannot compansate with the rising oxidant levels in case of an illness. Aging is not a disease itself, but the process of aging is accelerated by a disease.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"6 1","pages":"18-24"},"PeriodicalIF":0.7,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76048514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0