Turkish Journal of Biochemistry-turk Biyokimya Dergisi最新文献

{"title":"A Rare Hemoglobin Variant Which Interfered Hemoglobin A1C Result: Hemoglobin South Florida (β1(NA1)Val>Met, GTG>ATG; HBB: c.4G>A)","authors":"A. Celebiler, D. Güleç, N. Uzuncan","doi":"10.5505/TJB.2014.27247","DOIUrl":"https://doi.org/10.5505/TJB.2014.27247","url":null,"abstract":"Hb South Florida is a rare hemoglobin beta chain variant that is not associated with any clinical disorder. We report a heterozygous Hb South Florida (β1(NA1)Val>Met, GTG>ATG; HBB:c.4G>A) case determined during a premarital screening program. This hemoglobin variant can be identified with high performance liquid chromatography analysis confirmed with DNA sequencing. We emphasize in our study the importance of an interdisciplinary collaborative study at the provincial basis for the success of the hemoglobinopathy control program.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"16 1","pages":"226-230"},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87524889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Screening of phytate hydrolysis Bacillus sp. isolated from soil and optimization of the certain nutritional and physical parameters on the production of phytase","authors":"E. Demirkan, E. Baygin, A. Usta","doi":"10.5505/TJB.2014.26817","DOIUrl":"https://doi.org/10.5505/TJB.2014.26817","url":null,"abstract":"Objective: To isolate phytase producing Bacillus sp. from soil samples of Turkey, and opti- mize the growth conditions for maximum production of phytase. Material and Methods: The screening of isolates was performed on phytase screening me- dium. The best producer was selected. Phytase activity was determined by measur ing the amount of liberated inorganic phosphate. Optimal culture conditions and fermentation pa- rameters for phytase production were assessed. Results: 236 Bacillus sp. strains isolated. The best phytase producing strain showed higher enzyme yield in the presence of wheat bran and lactose as carbon source, meat extract as organic nitrogen source, CaCl 2 as metal source. 0.3% as phytate concentration was found to be best. In the physical parameters, the best results was obtained at 35°C, pH 7.5, 200 rpm as agitation rate, 2-4% as inoculum size and 48 h as inoculum age. A new medium was obtained by optimizing the incubation conditions of phytase production from Bacillus sp. EBD 9-1. In this medium, enzyme yield was enhanced 62% compared to basal medium. Conclusion: The present study suggests that the novel Bacillus sp. phytase enzyme may have wide industrial application, and can be used as an animal feed additive.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"47 1","pages":"206-214"},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90050693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Premarital Hemoglobinopathy Screening Study: Is Kadirli Prevalent Thalassemia Area Of Turkey?","authors":"K. Ulutas, F. Şahpaz, I. Sarici, Mahmut Uluganyan, Beril Akçimen, M. Celi̇k, Y. Can, İ. Kuru","doi":"10.5505/TJB.2014.90217","DOIUrl":"https://doi.org/10.5505/TJB.2014.90217","url":null,"abstract":"","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"33 1","pages":"357-361"},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84766150","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The evaluation of IMA as a cardiac ischemia marker in the cases of hypohemoglobinemia and hypoxemia due to blood loss","authors":"R. Şeker, A. Oğuz, S. Ozdemir, S. Demirtaş, M. Aylı, K. Mergen","doi":"10.5505/TJB.2014.04274","DOIUrl":"https://doi.org/10.5505/TJB.2014.04274","url":null,"abstract":"Objective: Although the mechanism is still not so clear, ischemia modified albumin is produced as a result of the occurrence of changes in metal binding regions on the albumin with the production of reactive oxygen species. But it is not known whether this change is the cause or the result of different states. This study is designed to investigate the correlation between hypohemoglobinemia, hypoxemia due to blood loss and IMA. Methods: The research is carried out with 83 patients (28 Male, 55 Female; mean age: 51±22 year) and 19 subject as control group (7Male, 12 Female; mean age 35±18 year). Patients were divided into three sub-groups according to their hemoglobin levels (<8, 8-10, 10-12 g/dL). Additionally, same patients were divided into second sub-group according to their follow-up status as outpatient (40 male, 15 female, mean age: 48 ± 20 year), postoperative (8 male, 11 female, mean age: 49 ± 24 year) and intensive care unit (6 male, 5 female; mean age: 64±20 year) patients. To determine the IMA levels the measurement method based on albumin cobalt binding assay was used. Results: In IMA levels, no significant difference is found between the mild (Hb: 10-12 g/dL) [(n=47; 0,61±0,23 ABSU)], moderate (Hb: 8-10 g/dL) [(n=26; 0.58±0.24 ABSU)] and severe (Hb: <8 g/dL) [(n=9; 0.62±0.12 ABSU)] anemia groups. However, because of acute blood loss related acute hypoxemia; IMA levels of the postoperative patients are statistically higher as compared to control group. As compared to control groups, no statistically significant difference is observed in the levels of the serum IMA in the outpatient group who had minimally invasive surgery and in the intensive care patient group. Conclusion: Our results suggest that; if mycardial oxygenization is questioned in cases of acute and heavy blood loss, ischemia modified albumin is not a valuable test for the indicator of the myocardial ischemia.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"60 1","pages":"221-225"},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85272667","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of resveratrol on Oxidant and Antioxidant Systems in Model of Rat Metabolic Syndrome","authors":"N. Ilhan","doi":"10.5505/TJB.2014.58569","DOIUrl":"https://doi.org/10.5505/TJB.2014.58569","url":null,"abstract":"Objective: In recent years, metabolic syndrome, which is a serious health problem, enhancing the effect of oxidative stress and lipid peroxidation, which is a natural antioxidant resveratrol, a polyphenol structure, the use of this area has brought. The present study aimed to evaluate the influence of resveratrol (RSV) treatment on heart, kidney and hepatic tissue malondialdehyde (MDA), nitric oxide (NO), superoxide dismutase (SOD) and catalase (CAT) levels in high fructose feeding rats which form an experimental metabolic syndrome model and compared to pre-treatment of metabolic syndrome group and the control group values. Methods: Wistar/albino rats (n=15) were used in the present study. Rats were divided equally into 3 groups as control, created group of metabolic syndrome and metabolic syndrome plus resveratrol treatment. At the end of the experimental period, tissue MDA, NO, SOD and CAT levels were studied manual spectrophotometric methods after homogenization of tissues. Results: The result of the study, a significant increase in heart and liver tissue MDA levels, and heart, kidney, liver tissue NO levels were observed in the metabolic syndrome group compared to the controls (p<0.05), following treatment with resveratrol significantly decreases were observed to be close to the control values. Kidney and liver tissue catalase activity statistically significant decrease in metabolic syndrome group compared to the controls, (p=0.019 and p=0.021, respectively), kidney and liver tissue catalase activity similar to control values after treatment with resveratrol, a significant increase was observed (p=0.011 and p=0.58), but changes in the activity of SOD has not been as significant as catalase (p>0.05). However, hepatic SOD activity of resveratrol treatment group compared with the control group, a significant increase was recorded in the treated group (p=0.011). Conclusion: These results suggest probably antioxidant effects of resveratrol to reduce oxidative stress in the liver and kidney tissue which is an important role in metabolism. Against tissue damage generated by exogenous fructose, resveratrol is effective in preventing tissue damage with a direct or indirect effect shows.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"62 3 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90695460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Adipose Tissue-Derived Inflammatory and Proangiogenic Cytokines on Proliferative Diabetic Retinopathy","authors":"Demet Yabanoğlu","doi":"10.5505/TJB.2014.38039","DOIUrl":"https://doi.org/10.5505/TJB.2014.38039","url":null,"abstract":"","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"41 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73523777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Comparative Assessment of α-Tocopherol Content and Antioxidant Activity of Some Fresh and Commercial Fruit Juices","authors":"T. Gönenç, H. Kayalar, T. Erdoğan, B. Kıvçak","doi":"10.5505/TJB.2014.36744","DOIUrl":"https://doi.org/10.5505/TJB.2014.36744","url":null,"abstract":"Aim: Fruits and vegetables are the main sources of antioxidant compounds and vitamins. In nature, α-tocopherol, is the most abundant and biologically active form of Vitamin E. The current study was aimed to investigate the antioxidant activity and α-tocopherol content of commercial and freshly squeezed fruit juices. Material and Methods: Apple, apricot and tomatoes fresh and commercial fruit juices were evaluated for their potential antioxidant activities using DPPH and ABTS + radical scavenging capacity assays. The total phenolic and flavonoid content of juices were determined by Folin- Ciocalteu and Aluminium chloride colorimetric methods respectively. The α-tocopherol con- tents of juices were investigated by HPLC-UV chromatographic method. Results: The highest α-tocopherol concentration was found in freshly squeezed tomatoes juice (0.0761±0.20mg/100g) the total phenolic content of freshly squeezed fruit juices mea- sured by Folin-Ciocalteu assay, ranged between 2.10-12.83mg gallic acid equivalents (mg GAE/100ml). The total flavonoid content of samples was expressed as quercetin equiva- lents (mg QE/100ml). The commercial tomatoes juice showed higher content of flavonoid (4.10±0.0126 mg QE/100ml), followed by freshly squeezed apple and apricot fruit juices. The fresh fruit juices of tomatoes showed the lowest content of flavonoid and highest content of total phenolic compounds. Freshly squeezed apple juice and apricot juice were found to exert higher antioxidant activities in ABTS+ and DPPH assays respectively. Conclusion: It was observed that α-tocopherol content and antioxidant capacities and total phenolic and flavonoid concentrations of freshly squeezed juices were higher than commer- cial ones.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"10 1","pages":"215-220"},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84342453","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prof. Dr. Mesude İşcan anısına","authors":"Nazmi Özer","doi":"10.5505/TJB.2014.25633","DOIUrl":"https://doi.org/10.5505/TJB.2014.25633","url":null,"abstract":"","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"80 1","pages":"244-244"},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77552751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Asymmetric dimethylarginine levels in Behçet's Disease","authors":"B. Ozturk, H. Vatansev, Alpaslan Taner, S. Yılmaz, M. Dağlı, A. Unlu","doi":"10.5505/TJB.2014.74436","DOIUrl":"https://doi.org/10.5505/TJB.2014.74436","url":null,"abstract":"Objective: Behcet's disease (BD) is a chronic, inflammatory, multisystem vasculitis and the etiology is not yet fully understood. Nitric Oxide (NO) is an important molecule for the vascular system which synthesised by the Nitric Oxide Synthase (NOS) enzyme. Asymmetric dimethylarginine (ADMA) is an endogenous inhibitor of NOS. Therefore, we aimed to investigate levels of the ADMA and its relation with exacerbations in BD patients. Methods: The subjects enrolled in this study were recruited from 34 patients with BD and 34 healthy controls. Serum levels of ADMA, arginine and citrulline were measured by HPLC with fluorescence detection. The ESR and CRP levels analyzed by routinely used methods. Results: In contrast to information in the literature, ADMA levels were found to be decreased in BD patients when compared to control. Inversely, Arginine and Citrulline levels were significantly increased in BD patients. Increased Arginine/ADMA ratios were found in the patient group. There was an inverse relation between ADMA and CRP levels. Arginine values were also correlated CRP and Citrulline levels. Conclusion: One of the reasons of high number of BD attacks in young age may be low levels of ADMA. Our study suggests that the reduced levels of ADMA in patients group may impose a possible preventive role to ADMA through decrease of NO-mediated inflammation and exacerbations of BD with aging.","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"40 1","pages":"362-367"},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76681079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determınatıon Of The Medıan Values Of Trıple Test Screening Parametres İn Yozgat Regıon","authors":"Gokhan Dogukan Akarsu","doi":"10.5505/TJB.2014.25338","DOIUrl":"https://doi.org/10.5505/TJB.2014.25338","url":null,"abstract":"","PeriodicalId":23355,"journal":{"name":"Turkish Journal of Biochemistry-turk Biyokimya Dergisi","volume":"20 1","pages":""},"PeriodicalIF":0.7,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85936231","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}