Tropical Medicine and Infectious Disease最新文献

{"title":"The Diagnostic Utility of PCR in FFPE Skin Biopsies with Inconclusive Histopathology for Suspected Cutaneous Leishmaniasis: A Pilot Study from Colombia.","authors":"Julio César Mantilla, Nathalia Andrea Bueno, Juan Pablo Alvarez, Maria Paula López, Martha Lucía Díaz","doi":"10.3390/tropicalmed10080232","DOIUrl":"10.3390/tropicalmed10080232","url":null,"abstract":"<p><p>Cutaneous Leishmaniasis (CL) is a tropical disease endemic in many regions of Latin America. Its clinical diagnosis is often supported by histopathological analysis of skin biopsies; however, parasite detection by microscopy can be challenging, particularly in chronic or pauciparasitic lesions, leading to inconclusive results.</p><p><strong>Objective: </strong>This study aimed to evaluate the utility of polymerase chain reaction (PCR) as a confirmatory diagnostic tool for CL in formalin-fixed, paraffin-embedded (FFPE) biopsies with inconclusive histopathology.</p><p><strong>Methods: </strong>We analyzed 16 FFPE skin biopsy samples from patients with clinical suspicion of CL. All cases underwent routine histopathological evaluation using hematoxylin and eosin staining and were classified as inconclusive. DNA was extracted and PCR was performed, targeting the ITS1 and miniexon regions of <i>Leishmania</i> spp.</p><p><strong>Results: </strong>PCR successfully amplified <i>Leishmania</i> DNA in 8 (50%) out of 16 samples when both targets were utilized, confirming infection. These findings highlight the additional benefits of molecular tools in cases with inconclusive histopathology, thereby enhancing diagnostic accuracy and enabling species-level identification in certain instances.</p><p><strong>Conclusions: </strong>PCR proved to be a valuable diagnostic complement to histopathology in clinically and histologically suspected cases of CL without visible parasites. Its implementation may improve diagnostic accuracy and timely treatment in endemic areas.</p>","PeriodicalId":23330,"journal":{"name":"Tropical Medicine and Infectious Disease","volume":"10 8","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12390503/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144970556","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prognostic Indicators of Severe Dengue Infection in Adult Patients in Thailand.","authors":"Patcharin Khamnuan, Surangrat Pongpan, Pantitcha Thanatrakolsri, Supa Vittaporn, Punnaphat Daraswang, Sirawan Samsee","doi":"10.3390/tropicalmed10080233","DOIUrl":"10.3390/tropicalmed10080233","url":null,"abstract":"<p><strong>Background: </strong>Dengue infection is a spreading vector borne disease with most severe infection-related fatalities occurring in adults. This study was conducted to explore prognostic indicators of dengue infection severity.</p><p><strong>Methods: </strong>This study included patients aged over 15 years who were diagnosed with dengue viral infection. Data were collected from nine hospitals across all regions of Thailand between January 2019 and December 2022. Diagnosis of dengue infection was confirmed by a positive result for the NS-1 antigen via RT-PCR, IgM antibody, or IgG antibody tests. Data including gender, age, BMI, underlying disease, clinical characteristics and laboratory findings were collected. Multivariable logistic regression with backward elimination was used to identify a set of prognostic factors.</p><p><strong>Results: </strong>The prognostic indicators of severe dengue were age < 55 years (OR = 6.13, <i>p</i> = 0.054), severe bleeding (bleeding from the gastrointestinal tract, hematemesis, melena, menorrhagia, or hematuria) (OR = 20.75, <i>p</i> < 0.001), pleural effusion (OR = 10.23, <i>p</i> < 0.001), and platelet ≤ 100,000 (/µL) (OR = 3.62, <i>p</i> = 0.035). These predictors were able to accurately estimate the severity of dengue infection with an area under the receiver operating curve (AuROC) of 0.836.</p><p><strong>Conclusions: </strong>The proposed four prognostic factors can be applied to predict severe dengue infections. These findings may inform the development of a risk scoring system to forecast severe dengue infection, early detection, and appropriate treatment during sickness.</p>","PeriodicalId":23330,"journal":{"name":"Tropical Medicine and Infectious Disease","volume":"10 8","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12390324/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144970623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Socioeconomic, Behavioural, and Protective Factors Influences on the Combined Prevention of HIV Infection Among Brazilian Amazon Men Who Have Sex with Men: A Cross-Sectional Study.","authors":"Thiago Vilhena Silva, Iaron Leal Seabra, Glenda Roberta Oliveira Naiff Ferreira, João Gabriel Alves da Luz, Cecília Conceição Viana, Lucas Barros de Paiva, Glauber Weder Dos Santos Silva, Caio Lacerda Dos Santos, Luiz Fernando Almeida Machado, Eliã Pinheiro Botelho","doi":"10.3390/tropicalmed10080231","DOIUrl":"10.3390/tropicalmed10080231","url":null,"abstract":"<p><p>We analysed the socioeconomic, behavioural, and protection factors (PFs) influences on the HIV combined prevention (CP) strategy among Brazilian Amazonian men who have sex with men (MSMs). PFs are resources that reduce the effect of adversity and help people maintain their well-being.</p><p><strong>Methods: </strong>Cross-sectional study employing a convenient sample of MSMs living in the metropolitan region of Belém. A questionnaire containing socioeconomic, behavioural, PFs, and behaviour/knowledge concerning CP questions was used. \"Behaviour/knowledge concerning CP\" was defined as a dependent variable and received a maximum score of 16 points. The Mann-Whitney and Kruskal-Wallis tests and multiple linear regression were employed.</p><p><strong>Results: </strong>Our sample comprised 384 MSMs scoring an average of 7.83 points (±1.9). Contributing to lower scores were \"not talking about sex life with confidants\", \"not talking with work colleagues about personal life and sexually transmissible infections\", and \"not participating in non-governmental organisations.\" On the other hand, \"not being happy in the neighbourhood of residency\" contributed to higher scores.</p><p><strong>Conclusion: </strong>Peer support and social inclusion are essential for increasing MSMs' access to CP.</p>","PeriodicalId":23330,"journal":{"name":"Tropical Medicine and Infectious Disease","volume":"10 8","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-08-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12389913/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144970561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Barcoding Quantitative PCR Assay to Distinguish Between <i>Aedes aegypti</i> and <i>Aedes sierrensis</i>.","authors":"Miguel Barretto, Annika Olson, Dereje Alemayehu, Ryan Clausnitzer, Eric J Haas-Stapleton","doi":"10.3390/tropicalmed10080230","DOIUrl":"10.3390/tropicalmed10080230","url":null,"abstract":"<p><p>The accurate identification of mosquito species is critical for effective mosquito surveillance and control, especially when presented with morphologically similar species like <i>Aedes aegypti</i> and <i>Aedes sierrensis</i>. Damaged specimens and morphologically similar life stages such as eggs and larvae make it difficult to distinguish <i>Aedes aegypti</i> from <i>Aedes sierrensis</i> using microscopy and taxonomic keys. To address this, the <i>AegySierr.</i>ID-qPCR assay, a multiplex quantitative PCR assay that utilizes single-nucleotide polymorphisms within the <i>mitochondrial cytochrome oxidase subunit I</i> gene, was developed to distinguish between these two species. The assay was tested on DNA extracted from the eggs, larvae, and adults of both species, as well as from environmental DNA (eDNA) collected from natural mosquito reproduction sites. It demonstrated a high diagnostic accuracy across multiple life stages, with a sensitivity exceeding 95% for most groups and specificity exceeding 90%, except for field-collected adult <i>Ae. sierrensis</i> (75%). For eDNA samples, the assay achieved 100% sensitivity and 94% specificity for samples classified as <i>Ae. sierrensis</i> and 91% sensitivity and 86% specificity for <i>Ae. aegypti</i>. A two-graph receiver operating characteristic analysis was also used as an alternate method with which to establish Ct thresholds for interpreting results from unknown samples. The <i>AegySierr.</i>ID-qPCR assay enables the rapid and sensitive identification of <i>Ae. aegypti</i> and <i>Ae. sierrensis</i> from specimens and eDNA, and may be of use in mosquito surveillance programs.</p>","PeriodicalId":23330,"journal":{"name":"Tropical Medicine and Infectious Disease","volume":"10 8","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12390609/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144970702","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seroprevalence of West Nile Virus in Blood Donors in Mainland Portugal.","authors":"Rafael Rocha, Elif Kurum, Rémi Charrel, Nazli Ayhan, Carla Maia","doi":"10.3390/tropicalmed10080229","DOIUrl":"10.3390/tropicalmed10080229","url":null,"abstract":"<p><p>The genus Orthoflavivirus includes several mosquito-borne pathogenic viruses, notably West Nile virus (WNV), which is endemic to the Mediterranean region. In Portugal, WNV circulation has been documented in equines, birds and mosquitoes, however human cases remain rare and no recent human seroprevalence studies have been conducted. This study aimed to estimate the national and regional seroprevalence of WNV among blood donors in mainland Portugal and explore associations with sociodemographic factors. A cross-sectional study conducted in 2022 included 3593 blood donors from across mainland Portugal. Serum samples were tested for WNV immunoglobulin G (IgG) by enzyme-linked immunosorbent assay (ELISA) and positive and borderline samples were confirmed using a virus neutralization test. Sociodemographic data were collected through a structured paper questionnaire. Statistical analyses, including multivariate logistic regression, identified factors associated with seropositivity. A total of 55 samples (1.5%) tested positive, and 21 samples (0.6%) were classified as borderline for WNV antibodies by ELISA. Of these, 47 were confirmed by viral neutralization, giving an estimated national seroprevalence of 1.4%. Significant regional variation was noted, with higher seroprevalence observed in the Beira Baixa, Grande Lisboa and Médio Tejo regions. Some seropositive individuals were identified in northern coastal regions such as Ave, Cávado and Área Metropolitana do Porto. In multivariate analysis, geographical area of residence was the only factor associated with seropositivity. This study highlights regions at potential higher risk for human WNV exposure, primarily in the southern half of Portugal. Continued and integrated surveillance is crucial to inform public health strategies to mitigate WNV transmission risks in these regions, as well as in other regions where WNV may be emerging as a relevant One Health concern. Implementing preventive measures for both animals and humans is critical to minimizing exposure and infection, and further confirmatory studies using virus neutralization tests will be important for refining these estimates.</p>","PeriodicalId":23330,"journal":{"name":"Tropical Medicine and Infectious Disease","volume":"10 8","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12390625/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144970600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Family Cluster of Imported Human <i>Brucella melitensis</i> Infection with Probable Breast Milk Transmission: A Case Series.","authors":"Christopher Loftus, Jessica Jervis, Victoria Owen, Tom Wingfield, Robert Ball, Waison Wong, Ceri Evans, Christopher Darlow, Francesca Liuzzi, Susan Batley, Rashika Fernando, Alessandro Gerada, Stephen D Woolley","doi":"10.3390/tropicalmed10080227","DOIUrl":"10.3390/tropicalmed10080227","url":null,"abstract":"<p><p>Human brucellosis is a zoonotic, bacterial infection caused by the intracellular, Gram-negative <i>Brucella</i> spp., which is common globally but rare in the United Kingdom, with approximately 20 imported cases per annum following travel to countries with high endemicity. Transmission typically occurs via the ingestion of infected animal products, including unpasteurised dairy products. Human-to-human transmission is rare, and routes include postpartum vertical transmission through breastfeeding. We report here on a familial cluster of three cases within a single UK-based Kurdish household of four, including a 11-month-old infant infected through the consumption of breast milk. Four months prior to presentation, the family had travelled together to northern Iraq for a 5-week holiday and all consumed local dairy products except for the children, including the 11-month-old, who was exclusively breastfed at the time. All three patients, including one adult male with complicated brucellosis, had a favourable outcome with medical therapy.: Brucellosis is an important differential diagnosis in returning travellers and specialist advice should be obtained early to prevent sequelae. It is also important for active case-finding, especially in family units with shared exposure. Paediatricians and adult physicians who may manage brucellosis should consider the possibility of vertical transmission in breastfeeding mothers.</p>","PeriodicalId":23330,"journal":{"name":"Tropical Medicine and Infectious Disease","volume":"10 8","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12390129/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144970733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mapping Evidence on the Regulations Affecting the Accessibility, Availability, and Management of Snake Antivenom Globally: A Scoping Review.","authors":"Ramsha Majeed, Janette Bester, Kabelo Kgarosi, Morné Strydom","doi":"10.3390/tropicalmed10080228","DOIUrl":"10.3390/tropicalmed10080228","url":null,"abstract":"<p><p>The World Health Organization (WHO) declared snakebite envenoming (SBE) as a neglected tropical disease in 2017. Antivenom is the gold standard of treatment, but many healthcare barriers exist, and hence, affected populations are often unable to access it. The challenge is further perpetuated by the lack of attention from national health authorities, poor regulatory systems and policies, and mismanagement of antivenom. This study aims to map the evidence regarding snake antivenom regulations globally and identify gaps in the literature to inform future research and policy. This review was conducted using the original Arksey and O'Malley framework by three independent reviewers, and the results were reported using the Preferred Reporting Items for Systematic reviews and Meta-Analysis Extension for Scoping Reviews (PRISMA-ScR). A search strategy was developed with assistance from a librarian, and six databases were searched: PubMed, SCOPUS, ProQuest Central, Africa Wide Web, Academic Search Output, and Web of Science. Screening was conducted independently by the reviewers, using Rayyan, and conflicts were resolved with discussions. A total of 84 articles were included for data extraction. The major themes that emerged from the included studies were regarding antivenom availability, accessibility, manufacturing, and regulations. The study revealed massive gaps in terms of policies governing antivenom management, especially in Asia and Africa. The literature does not offer sufficient evidence on management guidelines for antivenom in the endemic regions, despite identifying the challenges in supply. However, significant information from Latin America revealed self-sufficient production, involvement of national health bodies in establishing efficient regulations, effective distribution nationally and regionally, and technology sharing to reduce SBE-related mortality.</p>","PeriodicalId":23330,"journal":{"name":"Tropical Medicine and Infectious Disease","volume":"10 8","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12389931/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144970544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unmasking the Determinants of Loss to Follow-Up in Pulmonary Tuberculosis: A Study in Selangor, Malaysia.","authors":"Sumarni Mohd Ghazali, Kee Chee Cheong, Mohamad Nadzmi Md Nadzri, Nur'Ain Mohd Ghazali, Lim Mei Cheng, Lonny Chen Rong Qi Ahmad, Mohd Kamarulariffin Kamarudin, Nur Ar Rabiah Ahmad, Asrul Anuar Zulkifli, Cheong Yoon Ling, Qistina Ruslan, Sarbhan Singh, Balvinder Singh Gill, Asmah Razali, Nuur Hafizah Md Iderus","doi":"10.3390/tropicalmed10080226","DOIUrl":"10.3390/tropicalmed10080226","url":null,"abstract":"<p><p>Adherence to the 6-month tuberculosis (TB) treatment regimen is challenging due to its duration and side effects, with various factors influencing patient compliance. A retrospective cross-sectional study was conducted among newly diagnosed pulmonary TB (pTB) patients in Selangor, Malaysia, undergoing treatment in government primary care clinics and hospitals. Patients who were lost to follow-up (LTFU) within the first six months were determined by reviewing patient records and the national TB registry. Logistic regression analysis identified sociodemographic and clinical factors associated with LTFU. Of the 699 pTB patients, 55 (7.9%) were lost to follow-up. Factors significantly associated with LTFU included age (higher in 25-44-year-olds, adjusted odds ratio (aOR): 2.83), unmarried status (aOR: 2.17), lower education level (aOR: 6.13), being a smoker (aOR: 2.65), and unawareness of TB diagnosis (aOR: 38.14). A significant interaction was found between education level and awareness of diagnosis, with unawareness having a stronger association with LTFU among higher-educated patients. Young adults, those with a lower education level, unmarried individuals, smokers, and those unaware of their TB diagnosis are at higher risk of LTFU. These factors can be used for rapid risk assessment. Intensive counselling and health education at treatment initiation, particularly for at-risk patients, are crucial for preventing LTFU.</p>","PeriodicalId":23330,"journal":{"name":"Tropical Medicine and Infectious Disease","volume":"10 8","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12390508/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144970683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Snakebites in the Central American Region: More Government Attention Required.","authors":"Eduardo Alberto Fernandez, Ivan Santiago Fernandez Funez","doi":"10.3390/tropicalmed10080225","DOIUrl":"10.3390/tropicalmed10080225","url":null,"abstract":"<p><p>A review was conducted on snakebites in Central America. Information was extracted using the databases of PubMed, SciELO, and LILACS. Information included retrospective studies, case reports, and case series; in this way, valuable information was retrieved from limited sources. The identified studies comprised those discussing envenoming snakebites. Several species were identified, but three of them had major epidemiological features impacting envenoming by snakebites: <i>Bothrops asper</i>, <i>Crotalus simus</i>, and <i>Micrurus</i> sp. Adolescents and young adult males living in rural areas and engaged in agricultural activities were identified as the main victims of snakebites by clinical records. Symptoms of local damage in the bite sites included edema and skin and muscle necrosis. In addition, the cardiovascular system was affected, with symptoms like hypotension, bleeding, and coagulation disorders. Neurotoxicity causing sensitivity and motricity problems was also reported. For El Salvador, accidents caused by <i>Crotalus simus</i> and <i>Micrurus</i> spp. were given more attention due to their greater relevance. The role of <i>Bothrops</i> species was more relevant in the envenoming reported by other countries. Treatment was found to be provided based on antivenoms produced in Costa Rica, and the recovery of the patients depended on the time elapsed between the accident and the initial treatment in the healthcare system.</p>","PeriodicalId":23330,"journal":{"name":"Tropical Medicine and Infectious Disease","volume":"10 8","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-08-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12390078/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144970646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Contribution of Respiratory Syncytial Virus to Burden of Lower Respiratory Tract Infections: A Global Analysis of 204 Countries and Territories, 1990-2021.","authors":"Zhiwei Chen, Qiu Zhang, Junrong Li, Naihong Xie, Qingmei Zheng, Youzhen Lai, Xiaoyang Zhang","doi":"10.3390/tropicalmed10080223","DOIUrl":"10.3390/tropicalmed10080223","url":null,"abstract":"<p><p>Respiratory syncytial virus (RSV) is a major cause of morbidity and mortality from lower respiratory infections (LRIs) worldwide. This study analyzes trends in age-standardized death rates (ASDRs) and disability-adjusted life years (DALYs) due to RSV-induced LRIs from 1990 to 2019, using data from the Global Burden of Disease Study 2021 (GBD 2021). The findings show a gradual decline in deaths, ASDR, and DALYs throughout this period. However, these indicators were higher in men than in women, as well as more pronounced in sub-Saharan West Africa. Developed countries exhibited higher ASDR and DALY values than developing countries, with the highest burden observed among children and the elderly in low Socio-demographic Index (SDI) regions. Globally, RSV-induced LRIs have shown a significant reduction in burden, but interventions are still urgently needed-particularly in low SDI areas-to reduce the impact on vulnerable populations. Public health policies focusing on these high-risk groups are essential for addressing the remaining disparities in RSV-related morbidity and mortality.</p>","PeriodicalId":23330,"journal":{"name":"Tropical Medicine and Infectious Disease","volume":"10 8","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-08-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12390104/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144970399","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}