Transfusion最新文献

{"title":"Transfusion-transmitted babesiosis in the era of regional screening: Where are cases still emerging?","authors":"Rebecca Townsend, Paul Losco, Laura Tonnetti","doi":"10.1111/trf.70133","DOIUrl":"10.1111/trf.70133","url":null,"abstract":"<p><strong>Background: </strong>To reduce the risk of transfusion-transmitted babesiosis (TTB), the Food and Drug Administration (FDA) released guidance in 2019 requiring Babesia screening of all donations collected in 14 states on the East Coast and Upper Midwest (Connecticut, Delaware, Massachusetts, Maryland, Maine, New Hampshire, New Jersey, New York, Pennsylvania, Rhode Island, Virginia, and Vermont Minnesota and Wisconsin), plus Washington, DC. The American Red Cross (ARC) implemented Babesia blood donation screening in those areas in May 2020. Screening uses a transcription-mediated amplification (TMA) nucleic acid test (NAT) on pools of 16 whole-blood samples. NAT-reactive samples are tested for B. microti antibody by immunofluorescence assay (IFA).</p><p><strong>Case reports: </strong>From October 2021 to December 2025, the ARC has identified positive donors in four TTB cases. In all instances, the implicated donation was a Babesia untested red blood cell (RBC) unit collected in a non-endemic state where screening is not performed (West Virginia, Oregon, Georgia, and Michigan). All cases were travel-related: three donors reported travel to endemic states, while the fourth was a resident of an endemic state who donated during a vacation in a non-endemic state.</p><p><strong>Conclusion: </strong>The regional approach of Babesia blood testing has dramatically reduced the risk of TTB. However, the cases described in this report highlight the ongoing vulnerability posed by donor travel and underscore the need to continue monitoring the current geographic screening strategy.</p>","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":" ","pages":"871-875"},"PeriodicalIF":2.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146214222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel RHD allele caused by the RHD IVS4 + 2delT mutation identified in a Chinese Han male donor with a D-negative phenotype.","authors":"Xiao Hao, Ruirui Li, Lu Zhang, Jie Li","doi":"10.1111/trf.70136","DOIUrl":"10.1111/trf.70136","url":null,"abstract":"","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":" ","pages":"887-889"},"PeriodicalIF":2.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147373108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Building evidence for transfusion practice: A pilot study on bacterial adhesion and growth in red blood cell infusion sets.","authors":"Zoya Versey, Yuntong Kou, Paula van Vliet, Donna L Ledingham, Sandra Ramirez-Arcos","doi":"10.1111/trf.70104","DOIUrl":"10.1111/trf.70104","url":null,"abstract":"<p><strong>Background: </strong>Blood administration sets are changed after a prescribed time interval with variations in guidelines. In Canada, sets are changed every 4 h based on the theory of potential bacterial attachment. This study aimed to investigate whether bacteria adhere to the inner surfaces of blood administration sets during mimicked transfusions of red blood cell concentrates (RBCCs).</p><p><strong>Study design and methods: </strong>Serratia marcescens was used to test bacterial adhesion on a Primary PLUM Y-type Blood Set. Study phases (N ≥ 3): (i) RBCCs spiked with 10 CFU/mL and bacterial recovery after 4 h; (ii) and (iii) RBCCs spiked with 1 CFU/mL and bacterial recovery after 2 h (ii) and after 2 h and 24 h (iii); (iv) RBCCs inoculated with 0.1 CFU/mL and bacterial detection at 2-24 h. Bacterial adhesion was verified with dislodging, cultures, and scanning electron microscopy after completion of mimicked RBCC transfusions.</p><p><strong>Results: </strong>At 10 CFU/mL, S. marcescens was recovered in one of three assays; however, at 1 CFU/mL, bacterial recovery after 2 h was minimal (one of six assays), although proliferation at 24 h was significant. With initial loads of 0.1 CFU/mL, bacteria were only detected at 8 and 24 h, and in the filter, in one of five trials.</p><p><strong>Conclusion: </strong>This pilot report provides evidence of bacterial growth in transfusion sets. Data obtained with S. marcescens indicate that the use of blood administration sets could be extended from 4 to ≥6 h after the infusion of the first RBCC with no impact on patient safety. These findings align with existing guidelines endorsing a longer interval between changes of blood product administration sets.</p>","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":" ","pages":"689-699"},"PeriodicalIF":2.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13049246/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146158322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Steady-state mobilization with on-demand plerixafor after CD38 antibody-based induction in multiple myeloma patients.","authors":"Maximilian Alexander Röhnert, Anna Seifert, Karolin Trautmann-Grill, Christoph Röllig, Kristin Zimmer, Katharina Egger-Heidrich, Marius Bill, Lisa Heberling, Freya Schulze, Matthias Blechschmidt, Malte von Bonin, Martin Bornhäuser, Kristina Hölig, Raphael Teipel","doi":"10.1111/trf.70165","DOIUrl":"10.1111/trf.70165","url":null,"abstract":"<p><strong>Background: </strong>High-dose chemotherapy followed by autologous stem cell transplantation (ASCT) remains the standard of care for fit patients with newly diagnosed multiple myeloma (MM). The increasing use of CD38 antibody-based quadruplet induction regimens such as daratumumab-VTd (Dara-VTd) has raised concerns regarding impaired stem cell mobilization.</p><p><strong>Study design and methods: </strong>We conducted a retrospective single-center analysis comparing steady-state stem cell mobilization after Dara-VTd versus bortezomib-cyclophosphamide-dexamethasone (VCd) induction. Mobilization kinetics, plerixafor use, and CD34⁺ collection outcomes were evaluated. CD34⁺ counts prior to first apheresis were adjusted for plerixafor use (adjCD34⁺). Multivariate logistic regression was performed to identify predictors of mobilization success.</p><p><strong>Results: </strong>Among 153 patients, 85 received Dara-VTd and 68 received VCd. Despite significantly deeper responses after Dara-VTd (≥VGPR 81% vs. 42%; p < .01), these patients showed lower adjCD34⁺ counts prior to first apheresis (16 vs. 50/μL; p < .01) and required plerixafor more frequently (64% vs. 15%; p < .01). Nevertheless, cumulative CD34⁺ yields were comparable between Dara-VTd and VCd (6.4 vs. 6.0 × 10⁶ CD34⁺ cells/kg; p = .15), and target yields were achieved in the majority of patients proceeding to apheresis (90% vs. 94%). Dara-VTd induction, prior radiation, and high tumor burden were identified as independent negative predictors of mobilization success.</p><p><strong>Discussion: </strong>Although Dara-VTd induction is associated with impaired mobilization kinetics, successful steady-state mobilization remains feasible. On-demand plerixafor use overcomes mobilization deficits, supporting this approach in patients receiving CD38-based quadruplet induction therapy. Furthermore, follow-up analysis of stem cell graft utilization demonstrates a high proportion of collected but unused stem cell grafts in both cohorts.</p>","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":" ","pages":"751-762"},"PeriodicalIF":2.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13049232/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147487287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improved platelet recovery in cryopreserved platelets reconstituted in freeze-dried plasma.","authors":"Kristina Ehn, Per Sandgren, Klara Asplund Högelin, Agneta Wikman","doi":"10.1111/trf.70129","DOIUrl":"10.1111/trf.70129","url":null,"abstract":"<p><strong>Background: </strong>Cryopreserved platelets can support availability in settings where fresh platelets are inaccessible. Freeze-dried plasma (FDP) may serve as an alternative reconstitution medium to fresh frozen plasma (FFP) when plasma thawing is impractical. The effects of FDP on cryopreserved platelet quality remain underexplored. Thus, we aimed to evaluate its suitability.</p><p><strong>Study design and methods: </strong>Cryopreserved platelets were prepared from double-dose buffy-coat concentrates, split, prewashed, and frozen using dimethyl sulfoxide (DMSO) (5-6%). After thawing, paired units were resuspended in FFP (AB = 8) or FDP (OctaplasLG Powder, AB = 8). Platelet count and mean platelet volume were analyzed alongside blood gas parameters. Additionally, extracellular lactate dehydrogenase (LDH), sP-selectin, and VEGF were measured in the supernatant. Platelet markers (aggregation, adhesion, and activation) and microparticles were analyzed by flow cytometry. Clotting ability was evaluated using ROTEM.</p><p><strong>Results: </strong>After thawing, FDP units contained significantly more platelets than FFP units 206 ± 27 versus 176 ± 18 ×10⁹/units (p = .0006). LDH activity was lower (p = .040), whereas VEGF levels were higher (p = .0003) in the FDP group. Oxygen and carbon dioxide pressure differed significantly, yet pH was normal. Phenotypic expression and microparticle content demonstrated no significant differences. FDP units showed a shorter clotting time in ROTEM EXTEM (50 ± 5 vs. 58 ± 9 s, p = .035), although clot strength was similar.</p><p><strong>Discussion: </strong>FDP-reconstituted platelets were functionally comparable to those reconstituted with FFP, while demonstrating improved recovery and enhanced clot initiation, potentially due to differences in plasma composition. Given its logistical advantages, particularly in resource-limited settings, FDP represents a promising reconstitution medium for cryopreserved platelets.</p>","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":" ","pages":"803-813"},"PeriodicalIF":2.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13049259/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146202786","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neutrophil-reactive autoantibodies in blood donors: Can we expect TRALI?","authors":"Stefanie Jehle, Darvin Preuss, Yannick Waxmann, Anja Spies-Naumann, Silke Schmidt, Rick Kapur, Ulrich J Sachs, Behnaz Bayat","doi":"10.1111/trf.70125","DOIUrl":"10.1111/trf.70125","url":null,"abstract":"<p><strong>Background: </strong>Transfusion-related acute lung injury (TRALI) is a clinical condition characterized by acute non-cardiogenic pulmonary edema during or after transfusion. Despite several mitigation strategies, TRALI remains a leading cause of transfusion-related deaths. Tests on blood donors involved in TRALI, apart from leukocyte/endothelial reactive alloantibodies, can also reveal the presence of neutrophil autoantibodies. Here, we ask the question whether these autoantibodies could play a role in the development of TRALI.</p><p><strong>Materials and methods: </strong>Sera (n = 15) from donors involved in TRALI and not containing alloantibodies against human neutrophil antigens (HNAs) and human leukocyte antigens (HLAs) were collected if their serological characterization was suggestive for the presence of neutrophil-reactive autoantibodies. Sera (n = 12) were suitable for the study. Their ability to bind complement, to activate neutrophils, and to disrupt the endothelial barrier using albumin influx through an endothelial monolayer in a transwell chamber in the presence and absence of neutrophils was investigated.</p><p><strong>Results: </strong>None of the AIN sera, but 2/12 TRALI sera, induced reactive oxygen species (ROS) in neutrophils. Both of these TRALI sera induced endothelial barrier permeability in the presence, but not in the absence, of neutrophils. These two sera did not activate complement.</p><p><strong>Conclusion: </strong>Autoantibodies against neutrophils present in transfused blood components appear to be capable of contributing to TRALI in selected but not in all investigated cases, based on their ability to activate neutrophil ROS and induce endothelial cell permeability in vitro. Further analysis is required to understand the potential functional effects of neutrophil autoantibodies in TRALI.</p>","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":" ","pages":"794-802"},"PeriodicalIF":2.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13049269/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146132665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of the novel c.149-8C>G splicing variant in RHCE*02 that weakens C antigen expression.","authors":"Christophe Tournamille, Yann Fichou, Caroline Benech, Gabriel Neto Braga, Laurent Devaux, Raynald Flahaut, Aline Floch","doi":"10.1111/trf.70180","DOIUrl":"10.1111/trf.70180","url":null,"abstract":"","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":" ","pages":"890-892"},"PeriodicalIF":2.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13049271/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147475169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of RHD variant alleles in serologically D-negative Thai patients: Prevalence and novel discoveries.","authors":"Thunnakhon Sinwatcharaphirom, Pattarin Tangtanatakul, Tuangrat Kumar, Patrawadee Pitakpolrat, Suwanna Mekprasan, Manon Boonbangyang, Chureerat Phokaew, Phandee Watanaboonyongchareon, Ponlapat Rojnuckarin","doi":"10.1111/trf.70090","DOIUrl":"10.1111/trf.70090","url":null,"abstract":"<p><strong>Background: </strong>D-negative blood donors are rare (0.3%) in Thailand. Patients with Asian DEL are D-seronegative but can receive D-positive blood without anti-D alloimmunization. To improve blood management, this study aimed to determine screening methods for detecting RHD variant alleles in serologic D-negative Thai patients.</p><p><strong>Study design and methods: </strong>Serologic D-negative blood samples were subjected to adsorption/elution for the DEL phenotype. The Hybrid Rhesus box, RHD exon 4, and RHD1227A were analyzed using polymerase chain reaction (PCR) and Sanger sequencing for RHD1227A. For inconclusive results, whole genome sequencing (WGS) was conducted. Genetic variants on RHD and RHCE genes were confirmed using deletion-spanning PCR and Sanger sequencing.</p><p><strong>Results: </strong>Among 80 patients, 57 (71.3%) cases of total RHD gene deletion, 20 (25.0%) of Asian DEL hemizygosity, two (2.5%) of novel genetic variants, and one (1.2%) with an inconclusive result were identified. Two patients had a novel RHD exon 3 frameshift variant, c.441delG p.V147fs, producing a truncated protein. Serology of all patients with novel variants showed D-negative. The adsorption/elution testing showed 34.8% false positive and 9.4% false negative rates for Asian DEL.</p><p><strong>Discussion: </strong>Our study suggests that PCR and Sanger sequencing for Asian DEL is helpful for serologic D-negative Thai patients, while adsorption/elution is unreliable. WGS, if available, is useful to identify rare and new variants, whereas its cost is not worth for routine testing in the Asian population.</p>","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":" ","pages":"828-839"},"PeriodicalIF":2.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146053899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Delayed hemolytic transfusion reaction due to anti-Jk^a with agglutination on blood smear.","authors":"Elizabeth Cappello, Lerene Archer, Tarun Aurora, James Sikora, Jeanne E Hendrickson","doi":"10.1111/trf.70128","DOIUrl":"10.1111/trf.70128","url":null,"abstract":"","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":" ","pages":"648-650"},"PeriodicalIF":2.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146150623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A novel GCNT2 allele, c.869C>A (p.Ser290Tyr), identified in Chinese individuals.","authors":"Yanlian Liang, Qunjuan Zeng, Siqi Cai","doi":"10.1111/trf.70143","DOIUrl":"10.1111/trf.70143","url":null,"abstract":"","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":" ","pages":"882-884"},"PeriodicalIF":2.0,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147318349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}