Transfusion最新文献

{"title":"Coagulation factors in spray-dried plasma: A systematic review and meta-analysis.","authors":"Biswadev Mitra, Patrick J C Biggins, Denese C Marks, Michael C Reade","doi":"10.1111/trf.18255","DOIUrl":"10.1111/trf.18255","url":null,"abstract":"","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":" ","pages":"1012-1020"},"PeriodicalIF":2.5,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12088305/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144033851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of five novel ABO blood group alleles.","authors":"Rahel Kräuchi, Christine Henny, Bernd Schimanski, Christoph Niederhauser, Sofia Lejon Crottet","doi":"10.1111/trf.18247","DOIUrl":"10.1111/trf.18247","url":null,"abstract":"<p><strong>Background: </strong>Today we know more than 200 ABO variant alleles. Most ABO subtypes are caused by single nucleotide substitutions in exons 6 or 7, which encode for the catalytic domain of ABO glycosyltransferases. Understanding the molecular basis of these variants provides insights into the mechanisms responsible for the different phenotypes. We investigated the molecular background causing inconclusive ABO serology of five samples referred to our laboratory.</p><p><strong>Methods: </strong>The ABO phenotypes were determined by standard gel column agglutination and tube technique as well as flow cytometry. Molecular investigation included sequence-specific primer (SSP)-PCR and sequencing of all seven ABO exons containing the adjacent flanking intron regions (including the ABO regulatory regions) using published and in-house primers.</p><p><strong>Results: </strong>Serological and molecular analysis of five probands revealed diverse ABO blood group variations. Four probands showed weak A antigen expression and one proband weak B antigen expression. Proband 1 exhibited a c.952G>A (p.Val318Met) variant linked to the ABO*A1.01 background, while proband 2 carried a c.973T>C (p.Trp325Arg) and proband 3 a c.28+5G>A splice site variant associated with the same allele. Proband 4 revealed the c.407C>A (p.Thr136Lys) variant on an ABO*A2.01 background, and proband 5 had the c.860C>T (p.Ala287Val) variant associated with the ABO*B.01 background.</p><p><strong>Discussion: </strong>Here we show the serological and molecular analysis of five samples with serologic ABO blood grouping attenuations and/or discrepancies revealing novel A and B subgroup alleles. We report five novel variant alleles: ABO*A1.952A, ABO*A1.973C, ABO*A1.28+5A, ABO*A2.01.407A, and ABO*B1.860T.</p>","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":" ","pages":"858-862"},"PeriodicalIF":2.5,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144050293","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of cold-stored platelets using Golden Hour transport boxes: Function and quality.","authors":"Jamie Nash, Christine Saunders, Nicola Pearce, Michael Cahillane, Edward J Sayers, Victoria Stokes, David Rawlinson, Christopher Hingston, Tom Scorer, David Lockey, Chloe George","doi":"10.1111/trf.18197","DOIUrl":"10.1111/trf.18197","url":null,"abstract":"<p><strong>Background: </strong>The Emergency Medical Retrieval and Transfer Service in Wales provides prehospital critical care, including the transfusion of red blood cells and plasma. However, the logistical challenges of storing platelet concentrates (PCs) at 22°C with constant agitation limit their prehospital use. Cold-stored platelets (CSP) at 4°C without agitation offer a potential solution, demonstrating superior hemostatic capabilities in vitro and longer storage potential. This study investigated the viability of storing CSP in Golden Hour boxes for up to 96 h, followed by refrigeration, to enhance prehospital damage control resuscitation.</p><p><strong>Methods: </strong>Two buffy-coat-derived PCs were combined and split into two: one PC was refrigerated at 4°C ± 2°C without agitation (CSP) for 15 days, and the other was stored in a Golden Hour cold box from days 2 to 6 (GH-CSP) before being rotated back into refrigeration. In vitro assessments included aggregometry, thrombin generation, thromboelastography, and platelet activation via P-selectin and annexin V binding.</p><p><strong>Results: </strong>Temperature data demonstrated that a Golden Hour box can maintain a temperature of 2-6°C for up to 84 h with a CSP and two red cell concentrates. Platelet function was not significantly different between the two storage conditions. GH-CSP displayed increased annexin V binding on day 8 compared with CSP (32.31 ± 3.27% vs 26.36 ± 2.17%, p = .0026) and day 15 (41.76 ± 6.13% vs 38.41 ± 3.99%, p = .0199).</p><p><strong>Conclusion: </strong>CSP stored in a Golden Hour box was comparable with conventional CSP, suggesting this method may be viable for prehospital use.</p>","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":" ","pages":"S265-S275"},"PeriodicalIF":2.5,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12035987/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143693374","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Extended storage of leukoreduced whole blood for transfusion stored in CPD from 21 to 35 days to improve prehospital blood supply logistics in rural areas.","authors":"Hanne Braathen, Turid Helen Felli Lunde, Geir Strandenes, Torunn Oveland Apelseth","doi":"10.1111/trf.18170","DOIUrl":"10.1111/trf.18170","url":null,"abstract":"<p><strong>Background: </strong>Rural blood services with limited access to blood donors face challenges in supplying helicopter emergency medical services and local hospitals with leukoreduced whole blood. Extending the shelf life of whole blood can improve emergency preparedness during crises and conflicts and address critical transfusion needs and blood supply challenges. This study investigated whether the in vitro quality of stored leukoreduced whole blood in citrate-phosphate-dextrose (CPD) declined with extended storage for up to 35 days.</p><p><strong>Study design and methods: </strong>Twenty units of whole blood were collected in CPD (Imuflex, BB*LGQ456E6, Terumo BCT), leukoreduced with a platelet-sparing filter, and stored (2-6°C) for 35 days. The units were sampled on days 1, 21, 28, and 35, and hematology parameters, hemolysis, blood gas, glucose, coagulation, and thromboelastography were analyzed. Results were compared to historical controls (n = 26).</p><p><strong>Results: </strong>All units complied with European requirements throughout storage for 35 days, except one, which showed hemolysis of 0.9% on day 35. There was a decline in platelet count, hemostatic function, and plasma quality during storage. Comparisons with historical controls indicated few in vitro quality differences between CPD and CPDA-1 whole blood. Leukoreduction had a favorable effect on factor VIII concentration during storage.</p><p><strong>Conclusion: </strong>When comparing to European requirements, our results indicate that the shelf life of whole blood in CPD can be extended beyond 21 days. A risk-benefit analysis should be done to evaluate if shelf life can be extended to improve the availability of blood products for patients with severe bleeding in remote regions, crises, and war.</p>","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":" ","pages":"S204-S211"},"PeriodicalIF":2.5,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143484040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A new look at an old warning: Transfusing ABO group-specific red blood cells after large-volume low-titer group O whole blood in trauma resuscitation.","authors":"Mark H Yazer, Philip C Spinella, Emily Mayhew, John R Hess, Jennifer Gurney, J Michael Van Gent, Andrew P Cap","doi":"10.1111/trf.18189","DOIUrl":"10.1111/trf.18189","url":null,"abstract":"","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":" ","pages":"S331-S332"},"PeriodicalIF":2.5,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143568299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Finger thoracostomy: Significant risks and unproven benefits in prehospital settings.","authors":"Yael Mozer-Glassberg, Irina Radomislensky, Avi Benov, Ofer Almog","doi":"10.1111/trf.18198","DOIUrl":"10.1111/trf.18198","url":null,"abstract":"<p><strong>Background: </strong>Trauma is a leading cause of preventable death, with a significant portion of trauma deaths occurring in the prehospital setting. Interventions such as chest drainage may play a critical role in managing life-threatening conditions but face challenges due to poorly defined indications and reliance on anecdotal evidence rather than rigorous studies. Among chest drainage techniques, finger thoracostomy (FT) is a well-described, but controversial, method for decompressing the pleural cavity in emergencies like tension pneumothorax or hemothorax. Despite its simplicity and minimal equipment requirements, FT carries risks, including bleeding, infection, organ injury, temporary effects, and procedural failure.</p><p><strong>Study design and methods: </strong>This study examines eight FT procedures performed by Israel Defense Forces providers during the 2023-2024 \"Swords of Iron\" War in Gaza.</p><p><strong>Results: </strong>All patients sustained severe penetrating injuries, with mixed outcomes. One case highlighted severe complications, including infection and empyema weeks later. Additionally, challenges in maintaining up-to-date knowledge and adherence to protocols among reservists led to unauthorized FT procedures, emphasizing the dangers of improvisation without evidence.</p><p><strong>Discussion: </strong>Our findings, coupled with limited evidence for FT's effectiveness in prehospital settings, raise questions about its appropriateness in trauma care. These concerns highlight the critical importance of adhering to validated and evidence-based protocols in all aspects of medical practice. Deviating from such protocols not only introduces unnecessary risks but also undermines the standardization essential for optimal patient care. Further research is needed to clarify the role, if any, of FT in prehospital trauma management.</p>","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":" ","pages":"S98-S102"},"PeriodicalIF":2.5,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12035982/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143711401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hemolysis or vitamin B12? Unveiling the impostor.","authors":"Luz Y Sullivan, Joshua R Miller, Kenneth C Larson, Manish J Gandhi","doi":"10.1111/trf.18219","DOIUrl":"10.1111/trf.18219","url":null,"abstract":"","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":" ","pages":"797-798"},"PeriodicalIF":2.5,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143658646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detecting red blood cell protein antigens by tandem mass spectrometry.","authors":"Sandip Chavan, Ramanath Majumdar, Neha Joshi, Benjamin Madden, Jane Peterson, Craig T Tauscher, Jennifer E Schieber, Andrew P Norgan, Eapen K Jacob, Akhilesh Pandey, Justin E Juskewitch","doi":"10.1111/trf.18252","DOIUrl":"10.1111/trf.18252","url":null,"abstract":"<p><strong>Background: </strong>Tandem mass spectrometry (MS/MS) has become a common clinical laboratory testing modality has demonstrated success in distinguishing between small protein variations in transthyretin amyloidosis. Since many common clinically significant RBC antigens are also small protein variations, this study aimed to determine if MS/MS could correctly detect common RBC antigens within the Rh, Kell, Duffy, MNS, Kidd, Diego, and Lutheran blood group systems.</p><p><strong>Study design and methods: </strong>Residual samples from serotyped/genotyped blood donors at a hospital-based blood donation center from February to August 2021 were analyzed. RBC membrane protein preparations underwent protease digestion prior to MS/MS analysis for RhD, RhCE, Kell, atypical chemokine receptor 1 (Duffy), glycophorin A (MNS), glycophorin B (MNS), urea transporter 1 (Kidd), band 3 anion transport protein (Diego), and basal cell adhesion molecule (Lutheran). Untargeted liquid chromatography (LC)-MS/MS detected protein-specific peptides, while targeted LC-selected reaction monitoring (LC-SRM) detected RBC antigen-containing peptides.</p><p><strong>Results: </strong>Through the use of multiple proteases, untargeted LC-MS/MS detected protein-specific peptides in all but glycophorin B, with band 3 anion transport protein, basal cell adhesion molecule, Kell, and glycophorin A having the greatest protein sequence coverage. Targeted LC-SRM detected antigen-specific peptides for C^w, Di^a/Di^b, Kp^b, and Wr^a/Wr^b, which agreed with the donors' previous typing results.</p><p><strong>Discussion: </strong>MS/MS can successfully detect peptides from several blood group systems but only detected a subset of their common RBC antigens. Further sample enrichment and MS/MS detection improvements will need to occur before MS/MS could be considered a clinical RBC phenotyping modality.</p>","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":" ","pages":"968-977"},"PeriodicalIF":2.5,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12088880/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144011816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An assessment of laboratory changes during autologous whole blood transfusion training: A prospective, observational study.","authors":"Steven G Schauer, Juliette M Conte, Ian L Hudson, Jessica Mendez, Dayana Sifuentes, Fabiola Mancha, Melody A Martinez, Rocio J Huaman, Allyson A Arana, Jason B Corley, Andrew D Fisher, Michael A Meledeo, Brian J Kirkwood, Michael D April","doi":"10.1111/trf.18178","DOIUrl":"10.1111/trf.18178","url":null,"abstract":"<p><strong>Introduction: </strong>Hemorrhage is the leading cause of death after trauma. Blood transfusions are used to restore physiology but are stored in citrate preservative which can bind electrolytes, particularly calcium, leading to hypocalcemia. Few data exist on the changes that occur in humans because of whole blood donation/transfusion. We sought to determine the electrolyte changes that occur during whole blood donation/reinfusion.</p><p><strong>Methods: </strong>We conducted a prospective observational study of military personnel that served as donor/recipient for personnel performing autologous whole blood transfusion training. Trained research staff analyzed whole blood samples collected pre-donation, post-donation, and post-reinfusion of one autologous unit of whole blood. We used the i-STAT laboratory analyzer. Laboratory data are reported using means and standard deviations.</p><p><strong>Results: </strong>We prospectively enrolled 40 participants. The median age was 22 years (interquartile range [IQR] 20-26), and 95% were male. The median body mass index was 25.3 (IQR 23.0-29.1). There were four participants that reported use of dietary supplements: three reported taking creatine, and the other one reported taking fish oil, magnesium, multivitamin, creatine, caffeine, and beetroot powder. Mean iCa was 1.25 mmol/L (standard deviation [SD] 0.04) pre-donation, 1.26 (SD 0.04) post-donation, and 1.12 (SD 0.14) post-reinfusion. Comparatively, the mean potassium values were 3.90 mEq/L (SD 0.36), 4.10 (SD 0.62), and 4.09 (SD 0.72). Hemoglobin levels decreased by 0.34 g/dL (p < .001) from post-donation to post-reinfusion. Other labs had no significant changes.</p><p><strong>Conclusions: </strong>We noted a decrease in ionized calcium measurements post-reinfusion. Future studies should assess changes after larger volume transfusions and assess repletion methods.</p>","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":" ","pages":"S57-S62"},"PeriodicalIF":2.5,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143531893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of red blood cells storage duration on the development of acute kidney injury: A secondary analysis of the TRANSFUSE multicenter randomized controlled trial.","authors":"Cécile Aubron, Elizabeth M Moore, Bridget Ady, Eldho Paul, Maija Kaukonen, Lynne Murray, Jonathan Barrett, Matthew Bailey, Timothy Bowles, Sean Kelly, Claire Cattigan, David Cooper, David Ernest, David Evans, Jason Fletcher, Craig French, David Gattas, Dhaval Ghelani, Seton Henderson, Alex Kazemi, Bruce King, Peter Kruger, Janet Liang, Christopher MacIsaac, Colin McArthur, Alistair Nichol, Sandra Peake, Michael C Reade, Brent Richards, John Santamaria, Paul Young, Michael Bailey, Rinaldo Bellomo, D James Cooper, Zoe K McQuilten","doi":"10.1111/trf.18230","DOIUrl":"10.1111/trf.18230","url":null,"abstract":"<p><strong>Background: </strong>Red blood cell (RBC) transfusion is associated with an increased risk of acute kidney injury (AKI). The extent to which RBC storage affects this association is unclear. We aimed to evaluate the association between storage duration and the occurrence or worsening of any degree of AKI in critically ill patients.</p><p><strong>Study design and methods: </strong>In this pre-planned sub-study of the Standard Issue Transfusion versus Fresher Red-Cell Use in Intensive Care (TRANSFUSE) trial, which compared mortality of critically ill patients receiving either the freshest available allogenic RBC unit or standard availability RBC, patients hospitalized in one of the 31 participating sites and who did not have Stage 3 AKI according to the Kidney Disease Improving Global Outcomes (KDIGO) classification were eligible. The primary outcome was the cumulative proportion of patients who developed any degree of new AKI.</p><p><strong>Results: </strong>A total of 899 patients were included. The mean (SD) RBC storage duration was 22.4 (7.4) versus 11.9 (5.4) days in the standard issue RBC and short-storage RBC groups, respectively (p < 0.01). The percentage of patients who developed any stage of new AKI was similar between groups (24.8% in the standard issue RBC group versus 26.1% in the short-storage RBC group; p = 0.66) (Relative Risk 0.95, [95% confidence intervals 0.76-1.19]). There was no difference in secondary outcomes.</p><p><strong>Discussion: </strong>In this pre-planned sub-study of the TRANSFUSE trial, compared with using standard issue RBC, the transfusion of the freshest available RBC was not associated with a decrease in AKI.</p>","PeriodicalId":23266,"journal":{"name":"Transfusion","volume":" ","pages":"876-885"},"PeriodicalIF":2.5,"publicationDate":"2025-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143743124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}