IF 3.6 3区生物学

Traffic Pub Date : 2023-12-01 Epub Date: 2023-09-02 DOI: 10.1111/tra.12918

Chenyao Wang, Nikhil Sharma, Patricia M Kessler, Ganes C Sen

{"title":"Interferon induction by STING requires its translocation to the late endosomes.","authors":"Chenyao Wang, Nikhil Sharma, Patricia M Kessler, Ganes C Sen","doi":"10.1111/tra.12918","DOIUrl":"10.1111/tra.12918","url":null,"abstract":"To combat microbial infections, mammalian cells use a variety of innate immune response pathways to induce synthesis of anti-microbial proteins. The cGAS/STING pathway recognizes cytoplasmic viral or cellular DNA to elicit signals that lead to type I interferon and other cytokine synthesis. cGAMP, synthesized by DNA-activated cGAS, activates the ER-associated protein, STING, which oligomerizes and translocates to other intracellular membrane compartments to trigger different branches of signaling. We have reported that, in the ER, EGFR-mediated phosphorylation of Tyr245 of STING is required for its transit to the late endosomes, where it recruits and activates the transcription factor IRF3 required for IFN induction. In the current study, we inquired whether STING Tyr245 phosphorylation per se or STING's location in the late endosomes was critical for its ability to recruit IRF3 and induce IFN. Using pharmacological inhibitors or genetic ablation of proteins that are essential for specific steps of STING trafficking, we demonstrated that the presence of STING in the late endosomal membranes, even without Tyr245 phosphorylation, was sufficient for IRF3-mediated IFN induction.","PeriodicalId":23207,"journal":{"name":"Traffic","volume":" ","pages":"576-586"},"PeriodicalIF":3.6,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10840695/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10201531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Statistical modeling of mRNP transport in dendrites: A comparative analysis of β-actin and Arc mRNP dynamics. 树突中mRNP转运的统计模型：β-肌动蛋白和Arc mRNP动力学的比较分析。

IF 4.5 3区生物学

Traffic Pub Date : 2023-11-01 Epub Date: 2023-08-06 DOI: 10.1111/tra.12913

Hyerim Ahn, Xavier Durang, Jae Youn Shim, Gaeun Park, Jae-Hyung Jeon, Hye Yoon Park

{"title":"Statistical modeling of mRNP transport in dendrites: A comparative analysis of β-actin and Arc mRNP dynamics.","authors":"Hyerim Ahn, Xavier Durang, Jae Youn Shim, Gaeun Park, Jae-Hyung Jeon, Hye Yoon Park","doi":"10.1111/tra.12913","DOIUrl":"10.1111/tra.12913","url":null,"abstract":"Localization of messenger RNA (mRNA) in dendrites is crucial for regulating gene expression during long-term memory formation. mRNA binds to RNA-binding proteins (RBPs) to form messenger ribonucleoprotein (mRNP) complexes that are transported by motor proteins along microtubules to their target synapses. However, the dynamics by which mRNPs find their target locations in the dendrite have not been well understood. Here, we investigated the motion of endogenous β-actin and Arc mRNPs in dissociated mouse hippocampal neurons using the MS2 and PP7 stem-loop systems, respectively. By evaluating the statistical properties of mRNP movement, we found that the aging Lévy walk model effectively describes both β-actin and Arc mRNP transport in proximal dendrites. A critical difference between β-actin and Arc mRNPs was the aging time, the time lag between transport initiation and measurement initiation. The longer mean aging time of β-actin mRNP (~100 s) compared with that of Arc mRNP (~30 s) reflects the longer half-life of constitutively expressed β-actin mRNP. Furthermore, our model also permitted us to estimate the ratio of newly generated and pre-existing β-actin mRNPs in the dendrites. This study offers a robust theoretical framework for mRNP transport, which provides insight into how mRNPs locate their targets in neurons.","PeriodicalId":23207,"journal":{"name":"Traffic","volume":" ","pages":"522-532"},"PeriodicalIF":4.5,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10946522/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10319298","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anterograde trafficking of Toll-like receptors requires the cargo sorting adaptors TMED-2 and 7. Toll样受体的反向贩运需要货物分拣适配器TMED-2和7。

IF 4.5 3区生物学

Traffic Pub Date : 2023-11-01 Epub Date: 2023-07-26 DOI: 10.1111/tra.12912

Julia E J Holm, Sandro G Soares, Martyn F Symmons, Afiqah Saleh Huddin, Martin C Moncrieffe, Nicholas J Gay

引用次数: 0

Combination of hydrophobicity and codon usage bias determines sorting of model K⁺ channel protein to either mitochondria or endoplasmic reticulum. 疏水性和密码子使用偏差的组合决定了模型K+通道蛋白向线粒体或内质网的分选。

IF 4.5 3区生物学

Traffic Pub Date : 2023-11-01 Epub Date: 2023-08-14 DOI: 10.1111/tra.12915

Anja J Engel, Steffen Paech, Markus Langhans, James L van Etten, Anna Moroni, Gerhard Thiel, Oliver Rauh

{"title":"Combination of hydrophobicity and codon usage bias determines sorting of model K+ channel protein to either mitochondria or endoplasmic reticulum.","authors":"Anja J Engel, Steffen Paech, Markus Langhans, James L van Etten, Anna Moroni, Gerhard Thiel, Oliver Rauh","doi":"10.1111/tra.12915","DOIUrl":"10.1111/tra.12915","url":null,"abstract":"When the K+ channel-like protein Kesv from Ectocarpus siliculosus virus 1 is heterologously expressed in mammalian cells, it is sorted to the mitochondria. This targeting can be redirected to the endoplasmic reticulum (ER) by altering the codon usage in distinct regions of the gene or by inserting a triplet of hydrophobic amino acids (AAs) into the protein's C-terminal transmembrane domain (ct-TMD). Systematic variations in the flavor of the inserted AAs and/or its codon usage show that a positive charge in the inserted AA triplet alone serves as strong signal for mitochondria sorting. In cases of neutral AA triplets, mitochondria sorting are favored by a combination of hydrophilic AAs and rarely used codons; sorting to the ER exhibits the inverse dependency. This propensity for ER sorting is particularly high when a common codon follows a rarer one in the AA triplet; mitochondria sorting in contrast is supported by codon uniformity. Since parameters like positive charge, hydrophobic AAs, and common codons are known to facilitate elongation of nascent proteins in the ribosome the data suggest a mechanism in which local changes in elongation velocity and co-translational folding in the ct-TMD influence intracellular protein sorting.","PeriodicalId":23207,"journal":{"name":"Traffic","volume":" ","pages":"533-545"},"PeriodicalIF":4.5,"publicationDate":"2023-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10362730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dynamic tandem proximity-based proteomics-Protein trafficking at the proteome-scale. 基于动态串联邻近度的蛋白质组学蛋白质组学规模的蛋白质运输。

IF 4.5 3区生物学

Traffic Pub Date : 2023-11-01 Epub Date: 2023-08-15 DOI: 10.1111/tra.12914

Eric Chevet, Maria Antonietta De Matteis, Eeva-Liisa Eskelinen, Hesso Farhan

引用次数: 0

The role of extracellular vesicles in iron homeostasis and ferroptosis: Focus on musculoskeletal diseases. 细胞外囊泡在铁稳态和铁下垂中的作用:聚焦于肌肉骨骼疾病。

IF 4.5 3区生物学

Traffic Pub Date : 2023-09-01 DOI: 10.1111/tra.12905

Zhiwei Liao, Bide Tong, Zixuan Ou, Junyu Wei, Ming Lei, Cao Yang

{"title":"The role of extracellular vesicles in iron homeostasis and ferroptosis: Focus on musculoskeletal diseases.","authors":"Zhiwei Liao, Bide Tong, Zixuan Ou, Junyu Wei, Ming Lei, Cao Yang","doi":"10.1111/tra.12905","DOIUrl":"https://doi.org/10.1111/tra.12905","url":null,"abstract":"Iron homeostasis is crucial for maintaining proper cellular function, and its disruption is considered one of the pathogenic mechanisms underlying musculoskeletal diseases. Under conditions of oxidative stress, the accumulation of cellular iron overload and lipid peroxidation can lead to ferroptosis. Extracellular vesicles (EVs), serving as mediators in the cell‐to‐cell communication, play an important role in regulating the outcome of cell ferroptosis. Growing evidence has proven that EV biogenesis and secretion are tightly associated with cellular iron export. Furthermore, different sources of EVs deliver diverse cargoes to bring about phenotypic changes in the recipient cells, either activating or inhibiting ferroptosis. Thus, delivering therapies targeting ferroptosis through EVs may hold significant potential for treating musculoskeletal diseases. This review aims to summarize current knowledge on the role of EVs in iron homeostasis and ferroptosis, as well as their therapeutic applications in musculoskeletal diseases, and thereby provide valuable insights for both research and clinical practice.","PeriodicalId":23207,"journal":{"name":"Traffic","volume":"24 9","pages":"384-396"},"PeriodicalIF":4.5,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9944277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Crucial roles of Rab22a in endosomal cargo recycling. Rab22a在内体货物循环中的关键作用。

IF 4.5 3区生物学

Traffic Pub Date : 2023-09-01 DOI: 10.1111/tra.12907

Lingjie Kong, Shenghao Huang, Yuxuan Bao, Yingtong Chen, Chunyan Hua, Sheng Gao

{"title":"Crucial roles of Rab22a in endosomal cargo recycling.","authors":"Lingjie Kong, Shenghao Huang, Yuxuan Bao, Yingtong Chen, Chunyan Hua, Sheng Gao","doi":"10.1111/tra.12907","DOIUrl":"https://doi.org/10.1111/tra.12907","url":null,"abstract":"Endosomal cargo recycling lies at the heart of subcellular trafficking processes under the management of several Ras-related GTP-binding proteins (Rabs) which are coordinated by their upstream regulators and require their downstream effectors to display their functions. In this regard, several Rabs have been well-reviewed except Rab22a. Rab22a is a crucial regulator of vesicle trafficking, early endosome and recycling endosome formation. Notably, recent studies demonstrated the immunological roles of Rab22a, which are closely associated with cancers, infection and autoimmune disorders. This review provides an overview of the regulators and effectors of Rab22a. Also, we highlight the current knowledge of the role of Rab22a in endosomal cargo recycling, including the biogenesis of recycling tubules with the help of a complex with Rab22a at its core, and how different internalized cargo chooses different recycling routes thanks to the cooperation of Rab22a, its effectors and its regulators. Of note, contradictions and speculation related to endosomal cargo recycling that Rab22a brings impacts on are also discussed. Finally, this review endeavors to briefly introduce the various events impacted by Rab22a, particularly focusing on the commandeered Rab22a-associated endosomal maturation and endosomal cargo recycling, in addition to the extensively investigated oncogenic role of Rab22a.","PeriodicalId":23207,"journal":{"name":"Traffic","volume":"24 9","pages":"397-412"},"PeriodicalIF":4.5,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9944280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Cholangiocytes express an isoform of soluble adenylyl cyclase that is N-linked glycosylated and secreted in extracellular vesicles. 胆管细胞表达一种可溶性腺苷酸环化酶的异构体，该酶被n链糖基化并在细胞外囊泡中分泌。

IF 4.5 3区生物学

Traffic Pub Date : 2023-09-01 DOI: 10.1111/tra.12904

Simei Go, Hang Lam Li, Jung-Chin Chang, Arthur J Verhoeven, Ronald P J Oude Elferink

{"title":"Cholangiocytes express an isoform of soluble adenylyl cyclase that is N-linked glycosylated and secreted in extracellular vesicles.","authors":"Simei Go, Hang Lam Li, Jung-Chin Chang, Arthur J Verhoeven, Ronald P J Oude Elferink","doi":"10.1111/tra.12904","DOIUrl":"https://doi.org/10.1111/tra.12904","url":null,"abstract":"Soluble adenylyl cyclase (sAC)-derived cAMP regulates various cellular processes; however, the regulatory landscape mediating sAC protein levels remains underexplored. We consistently observed a 85 kD (sAC85 ) or 75 kD (sAC75 ) sAC protein band under glucose-sufficient or glucose-deprived states, respectively, in H69 cholangiocytes by immunoblotting. Deglycosylation by PNGase-F demonstrated that both sAC75 and sAC85 are N-linked glycosylated proteins with the same polypeptide backbone. Deglycosylation with Endo-H further revealed that sAC75 and sAC85 carry distinct sugar chains. We observed release of N-linked glycosylated sAC (sACEV ) in extracellular vesicles under conditions that support intracellular sAC85 (glucose-sufficient) as opposed to sAC75 (glucose-deprived) conditions. Consistently, disrupting the vesicular machinery affects the maturation of intracellular sAC and inhibits the release of sACEV into extracellular vesicles. The intracellular turnover of sAC85 is extremely short (t1/2 ~30 min) and release of sACEV in the medium was detected within 3 h. Our observations support the maturation and trafficking in cholangiocytes of an N-linked glycosylated sAC isoform that is rapidly released into extracellular vesicles.","PeriodicalId":23207,"journal":{"name":"Traffic","volume":"24 9","pages":"413-430"},"PeriodicalIF":4.5,"publicationDate":"2023-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9944282","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Deacidification of endolysosomes by neuronal aging drives synapse loss. 神经元老化导致内溶酶体脱酸，导致突触丢失。

IF 4.5 3区生物学

Traffic Pub Date : 2023-08-01 DOI: 10.1111/tra.12889

Tatiana Burrinha, César Cunha, Michael J Hall, Mafalda Lopes-da-Silva, Miguel C Seabra, Cláudia Guimas Almeida

{"title":"Deacidification of endolysosomes by neuronal aging drives synapse loss.","authors":"Tatiana Burrinha, César Cunha, Michael J Hall, Mafalda Lopes-da-Silva, Miguel C Seabra, Cláudia Guimas Almeida","doi":"10.1111/tra.12889","DOIUrl":"https://doi.org/10.1111/tra.12889","url":null,"abstract":"Previously, we found that age-dependent accumulation of beta-amyloid is not sufficient to cause synaptic decline. Late-endocytic organelles (LEOs) may be driving synaptic decline as lysosomes (Lys) are a target of cellular aging and relevant for synapses. We found that LAMP1-positive LEOs increased in size and number and accumulated near synapses in aged neurons and brains. LEOs' distal accumulation might relate to the increased anterograde movement in aged neurons. Dissecting the LEOs, we found that late-endosomes accumulated while there are fewer terminal Lys in aged neurites, but not in the cell body. The most abundant LEOs were degradative Lys or endolysosomes (ELys), especially in neurites. ELys activity was reduced because of acidification defects, supported by the reduction in v-ATPase subunit V0a1 with aging. Increasing the acidification of aged ELys recovered degradation and reverted synaptic decline, while alkalinization or v-ATPase inhibition, mimicked age-dependent Lys and synapse dysfunction. We identify ELys deacidification as a neuronal mechanism of age-dependent synapse loss. Our findings suggest that future therapeutic strategies to address endolysosomal defects might be able to delay age-related synaptic decline.","PeriodicalId":23207,"journal":{"name":"Traffic","volume":"24 8","pages":"334-354"},"PeriodicalIF":4.5,"publicationDate":"2023-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10126393","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Genetic disruption of mammalian endoplasmic reticulum-associated protein degradation: Human phenotypes and animal and cellular disease models. 哺乳动物内质网相关蛋白降解的遗传破坏:人类表型和动物及细胞疾病模型。

IF 4.5 3区生物学

Traffic Pub Date : 2023-08-01 DOI: 10.1111/tra.12902

Sally Badawi, Feda E Mohamed, Divya Saro Varghese, Bassam R Ali

引用次数: 0

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