STEM CELLS最新文献_第10页

Brain pericytes derived from human pluripotent stem cells retain vascular and phagocytic functions under hypoxia. 由人多能干细胞衍生的脑周细胞在缺氧条件下仍能保持血管功能和吞噬功能。

IF 4.8 2区医学

STEM CELLS Pub Date : 2025-10-16 DOI: 10.1093/stmcls/sxaf055

Mingzi Zhang, Youbin Kim, Allison Bosworth, Julia Tcw, Lina R Nih, Kassandra Kisler, Abhay P Sagare, Ruslan Rust

{"title":"Brain pericytes derived from human pluripotent stem cells retain vascular and phagocytic functions under hypoxia.","authors":"Mingzi Zhang, Youbin Kim, Allison Bosworth, Julia Tcw, Lina R Nih, Kassandra Kisler, Abhay P Sagare, Ruslan Rust","doi":"10.1093/stmcls/sxaf055","DOIUrl":"10.1093/stmcls/sxaf055","url":null,"abstract":"Background: The integrity and function of the blood‑brain barrier (BBB) are largely regulated by pericytes. Pericyte deficiency leads to BBB breakdown and neurological dysfunction in major neurological disorders including stroke and Alzheimer's disease (AD). Transplantation of pericytes derived from induced pluripotent stem cells (iPSC‑PC) has been shown to restore the BBB and improve functional recovery in mouse models of stroke and pericyte deficiency. However, the molecular profile and functional properties of iPSC‑PC under hypoxic conditions, similar to those found in ischemic and neurodegenerative diseases remain largely unexplored.Methods: We examined iPSC‑PC under hypoxia to assess molecular marker expression, proliferation, ability to home to brain vessels, and uptake of amyloid beta (Aβ).Results: iPSC‑PC under severe hypoxia retain essential functional properties, including key molecular markers, proliferation rates, and the ability to migrate to host brain vessels via function‑associated PDGFRB‑PDGF‑BB signaling. Additionally, we show that iPSC‑PC exhibit similar clearance of Aβ neurotoxins from AD mouse brain sections under both normoxic and hypoxic conditions.Conclusions: These findings suggest that iPSC‑PC functions are largely resilient to hypoxia, highlighting their potential as a promising cell source for treating ischemic and neurodegenerative disorders.","PeriodicalId":231,"journal":{"name":"STEM CELLS","volume":" ","pages":""},"PeriodicalIF":4.8,"publicationDate":"2025-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12573254/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144751874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Resistance to naive and formative pluripotency conversion in RSeT human embryonic stem cells. RSeT人胚胎干细胞对初始和形成性多能转化的抗性。

IF 3.6 2区医学

STEM CELLS Pub Date : 2025-09-17 DOI: 10.1093/stmcls/sxaf056

Kevin G Chen, Kory R Johnson, Kyeyoon Park, Dragan Maric, Forest Yang, Wenfang Liu, Yang C Fann, Barbara S Mallon, Pamela G Robey

{"title":"Resistance to naive and formative pluripotency conversion in RSeT human embryonic stem cells.","authors":"Kevin G Chen, Kory R Johnson, Kyeyoon Park, Dragan Maric, Forest Yang, Wenfang Liu, Yang C Fann, Barbara S Mallon, Pamela G Robey","doi":"10.1093/stmcls/sxaf056","DOIUrl":"10.1093/stmcls/sxaf056","url":null,"abstract":"One of the most important properties of human embryonic stem cells (hESCs) is their ability to exist in primed and naive pluripotent states. Our previous meta-analysis indicated the existence of heterogeneous pluripotent states derived from diverse naive protocols. In this study, we characterized a commercial, RSeT-based pluripotent state under various growth conditions. Notably, RSeT hESCs can circumvent the hypoxic growth conditions required by naive hESCs, although some RSeT cells (eg, H1 cells) exhibit much lower single-cell plating efficiency and display altered or significantly retarded cell growth under both normoxia and hypoxia. Importantly, RSeT hPSCs lack many transcriptomic hallmarks of naive and formative pluripotency (the phase between naive and primed states). Integrative transcriptome analysis suggests that our primed and RSeT hESCs are similar to the early stage of post-implantation embryos, in line with previously reported primary hESCs and early hESC cultures. Moreover, RSeT hESCs do not express naive surface markers such as SUSD2 and CD75 at significant levels. At the biochemical level, RSeT hESCs show differential dependence on FGF2 and co-independency on both Janus kinase (JAK) and TGFβ signaling in a cell line-specific manner. Thus, RSeT hESCs represent a previously unrecognized pluripotent state downstream of naive pluripotency. Our data suggest that human naive pluripotent potentials may be restricted in RSeT medium, which sustains FGF2 activity. Hence, this study provides new insights into pluripotent state transitions in vitro.","PeriodicalId":231,"journal":{"name":"STEM CELLS","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12471354/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144937402","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In reply to the letter to the editor from Jin et al: critical insights into the role of miR-290 and miR-302 clusters in iPSC reprogramming. 在回复Jin等人给编辑的信中：对miR-290和miR-302集群在iPSC重编程中的作用的关键见解。

IF 4.8 2区医学

STEM CELLS Pub Date : 2025-09-17 DOI: 10.1093/stmcls/sxaf046

Robert Blelloch

引用次数: 0

15-PGDH inhibition enhances hematopoietic regeneration during aging. 15-PGDH抑制可促进衰老过程中的造血再生。

IF 4.8 2区医学

STEM CELLS Pub Date : 2025-09-17 DOI: 10.1093/stmcls/sxaf047

Rahul Chaudhary, Brittany A Cordova, Marcus Hong, Bailey R Klein, Lyannah A Contreras, Ritisha Rashmil, Filip Goshevski, Julianne N P Smith, Derek J Taylor, Andrew A Pieper, Sanford Markowitz, Amar B Desai

{"title":"15-PGDH inhibition enhances hematopoietic regeneration during aging.","authors":"Rahul Chaudhary, Brittany A Cordova, Marcus Hong, Bailey R Klein, Lyannah A Contreras, Ritisha Rashmil, Filip Goshevski, Julianne N P Smith, Derek J Taylor, Andrew A Pieper, Sanford Markowitz, Amar B Desai","doi":"10.1093/stmcls/sxaf047","DOIUrl":"10.1093/stmcls/sxaf047","url":null,"abstract":"Hematopoietic aging is characterized by diminished stem cell regenerative capacity and an increased risk of hematologic dysfunction. We previously identified that the prostaglandin-degrading enzyme 15-hydroxyprostaglandin dehydrogenase (15-PGDH) regulates hematopoietic stem cell (HSC) activity. Here, we expand on this work and demonstrate that in aged mice: (1) 15-PGDH expression and activity remain conserved in the bone marrow (BM) and spleen, suggesting that it remains a viable therapeutic target in aging; (2) prolonged PGDH inhibition (PGDHi) significantly increases the frequency and number of phenotypic hematopoietic stem and progenitor cells across multiple compartments, with transcriptional changes indicative of enhanced function; (3) PGDHi-treated BM enhances short-term hematopoietic recovery following transplantation, leading to improved peripheral blood output and accelerated multilineage reconstitution; and (4) PGDHi confers a competitive advantage in primary hematopoietic transplantation while mitigating age-associated myeloid bias in secondary transplants. Notably, these effects occur without perturbing steady-state blood production, suggesting that PGDHi enhances hematopoiesis under regenerative conditions while maintaining homeostasis. Our work identifies PGDHi as a translatable intervention to rejuvenate aged HSCs and mitigate hematopoietic decline.","PeriodicalId":231,"journal":{"name":"STEM CELLS","volume":" ","pages":""},"PeriodicalIF":4.8,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12449622/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144558663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical evidence and critical review of mesenchymal stromal cells for corneal and ocular surface diseases. 间充质间质细胞治疗角膜和眼表疾病的临床证据和关键综述

IF 4.8 2区医学

STEM CELLS Pub Date : 2025-09-17 DOI: 10.1093/stmcls/sxaf048

Grace C Tu, Seyyedehfatemeh Ghalibafan, Farshad Abedi, Charlotte E Joslin, Reza Dana, Peiman Hematti, Ali R Djalilian

{"title":"Clinical evidence and critical review of mesenchymal stromal cells for corneal and ocular surface diseases.","authors":"Grace C Tu, Seyyedehfatemeh Ghalibafan, Farshad Abedi, Charlotte E Joslin, Reza Dana, Peiman Hematti, Ali R Djalilian","doi":"10.1093/stmcls/sxaf048","DOIUrl":"10.1093/stmcls/sxaf048","url":null,"abstract":"Mesenchymal stromal cells (MSCs), owing to their regenerative and immunomodulatory properties, have emerged as a potential therapeutic option for disorders affecting the cornea and ocular surface. Early-phase clinical studies have begun to demonstrate the safety and, to some extent, efficacy of MSC-based therapies in conditions such as dry eye disease, persistent corneal epithelial defects, ocular chemical injuries, corneal scarring, keratoconus, and limbal stem cell deficiency. However, evidence from some studies suggests that MSC-related improvements may be short-lived. Currently, the appropriate clinical indications, delivery methods, and long-term outcomes remain unclear, necessitating further laboratory and clinical investigations. In this review, we summarize published and ongoing clinical studies on the therapeutic applications of MSCs for ocular surface diseases, including our own group's experience. We critically evaluate the strengths and limitations of existing studies and highlight gaps and opportunities in this evolving field.","PeriodicalId":231,"journal":{"name":"STEM CELLS","volume":" ","pages":""},"PeriodicalIF":4.8,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12471346/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144599019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Critical insights into the role of miR-290 and miR-302 clusters in iPSC reprogramming. 对miR-290和miR-302集群在iPSC重编程中的作用的关键见解。

IF 3.6 2区医学

STEM CELLS Pub Date : 2025-09-17 DOI: 10.1093/stmcls/sxaf045

Shan Tao, Xianghui Zhang, Chengqiang Jin

引用次数: 0

Consistent self-organized emergence of hyaline cartilage in human induced pluripotent stem cell-derived multi-tissue organoids. 在hipsc衍生的多组织类器官中一致的自组织出现透明软骨。

IF 4.8 2区医学

STEM CELLS Pub Date : 2025-09-17 DOI: 10.1093/stmcls/sxaf043

Huzefa I Husain, Manci Li, Juan E Abrahante, Natalia Calixto Mancipe, Amanda Vegoe, Yi Wen Chai, Beth Lindborg, Marc Tompkins, Brenda Ogle, Peter A Larsen, Timothy D O'Brien, Ferenc Tóth

{"title":"Consistent self-organized emergence of hyaline cartilage in human induced pluripotent stem cell-derived multi-tissue organoids.","authors":"Huzefa I Husain, Manci Li, Juan E Abrahante, Natalia Calixto Mancipe, Amanda Vegoe, Yi Wen Chai, Beth Lindborg, Marc Tompkins, Brenda Ogle, Peter A Larsen, Timothy D O'Brien, Ferenc Tóth","doi":"10.1093/stmcls/sxaf043","DOIUrl":"10.1093/stmcls/sxaf043","url":null,"abstract":"Existing protocols for in vitro hyaline cartilage production utilizing human induced pluripotent stem cells (hiPSCs) have several challenges including a complex culturing process that uses undefined culture media, phenotypic instability, and batch-to-batch variability of the cell product. Here, our primary objective is to describe a simple, xeno- and feeder-free protocol for the generation of hyaline cartilage utilizing multi-tissue organoids (MTOs). We investigated gene regulatory networks during hiPSC-MTO differentiation using RNA sequencing and bioinformatics analyses, as well as histological and immunohistochemical methods. Interplays between bone morphogenetic protein (BMP) and neural fibroblast growth factor (FGF) pathways associated with the phenotypic transition of MTOs are described. Comparisons across transcriptomes revealed that the expression of chondrocyte-specific genes in MTOs correlates strongly with fetal lower limb chondrocytes. Single-cell RNA sequencing findings confirmed that the majority of cells belonged to the chondrogenic lineage and that they were similar across MTO batches, suggesting uniformity of the culture process. Collectively, these findings demonstrate the consistent emergence of hyaline cartilage in MTOs and the molecular pathways that govern this process, thereby establishing an accessible source of functional chondrocytes for future therapeutic evaluations.","PeriodicalId":231,"journal":{"name":"STEM CELLS","volume":" ","pages":""},"PeriodicalIF":4.8,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12463443/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144511284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential effects of various cytokine priming on T-cell suppression and migration in canine mesenchymal stem cells. 不同细胞因子启动对犬间充质干细胞T细胞抑制和迁移的差异影响。

IF 3.6 2区医学

STEM CELLS Pub Date : 2025-09-17 DOI: 10.1093/stmcls/sxaf044

Chan-Hee Jo, Sang-Yun Lee, Young-Bum Son, Won-Jae Lee, Hyeon-Jeong Lee, Seong-Ju Oh, Chae-Yeon Hong, Yong-Ho Choe, Sung-Lim Lee

{"title":"Differential effects of various cytokine priming on T-cell suppression and migration in canine mesenchymal stem cells.","authors":"Chan-Hee Jo, Sang-Yun Lee, Young-Bum Son, Won-Jae Lee, Hyeon-Jeong Lee, Seong-Ju Oh, Chae-Yeon Hong, Yong-Ho Choe, Sung-Lim Lee","doi":"10.1093/stmcls/sxaf044","DOIUrl":"10.1093/stmcls/sxaf044","url":null,"abstract":"Mesenchymal stem cells (MSCs) have been widely studied for treating immune-mediated diseases due to their immunomodulatory abilities. Recent studies have shown that priming MSCs with inflammatory cytokines can enhance these functions, yet the optimal priming conditions for canine MSCs remain poorly defined. In this study, we investigated the effects of priming canine adipose tissue-derived MSCs (cAMSCs) with inflammatory cytokines IFN-γ, TNF-α, and IL-17 at various concentrations (10, 20, and 50 ng/mL) to evaluate their immunomodulatory and migratory capacities. Of the 3 cytokines evaluated, only IFN-γ priming significantly enhanced the expression of immunosuppressive genes IDO and PD-L1, and robustly suppressed T-cell proliferation across all concentrations compared to naïve cAMSCs in both direct co-culture and indirect (conditioned medium) assays. TNF-α priming significantly increased HGF expression and promoted cell cycle progression, while IL-17 priming upregulated COX2 and TGF-β expression; however, both exhibited limited immunomodulatory effects compared to IFN-γ. In addition, IFN-γ induced strong expression of adhesion and migration-related genes, including E-CADHERIN, ICAM1, and VCAM1, and promoted cAMSCs migration in a wound healing assay. Despite increasing MHC II, IFN-γ did not induce CD80, preserving the low immunogenic profile of cAMSCs. These findings support IFN-γ priming as the most effective strategy to enhance the immunomodulatory and migratory functions of cAMSCs, offering substantial potential for MSC-based therapies in veterinary medicine.","PeriodicalId":231,"journal":{"name":"STEM CELLS","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144590125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single-cell analysis of heterogeneity and molecular changes in cultured corneal epithelial stem cells during serial passage. 培养角膜上皮干细胞序列传代过程中异质性和分子变化的单细胞分析。

IF 3.6 2区医学

STEM CELLS Pub Date : 2025-09-17 DOI: 10.1093/stmcls/sxaf041

Usanee Reinprayoon, Supaporn Khramchantuk, Natthida Kittimawikrom, Praewphan Ingrungruanglert, Sarinya Phodang, Thanakorn Jaemthaworn, Sira Sriswasdi, Nipan Israsena

{"title":"Single-cell analysis of heterogeneity and molecular changes in cultured corneal epithelial stem cells during serial passage.","authors":"Usanee Reinprayoon, Supaporn Khramchantuk, Natthida Kittimawikrom, Praewphan Ingrungruanglert, Sarinya Phodang, Thanakorn Jaemthaworn, Sira Sriswasdi, Nipan Israsena","doi":"10.1093/stmcls/sxaf041","DOIUrl":"10.1093/stmcls/sxaf041","url":null,"abstract":"The maintenance of corneal epithelial homeostasis relies on limbal stem cells (LSCs) located at the limbus. Although short-term cultured LSC transplantation effectively treats LSC deficiency, prolonged culture leads to stemness loss and abortive colony formation, and the mechanisms remain elusive. In this study, we employed single-cell transcriptomics to investigate LSC population dynamics and changes in gene expression during extended serial culture. Transcriptomic data from 22 708 cells revealed 19 clusters, identifying 3 distinct limbal progenitor populations (Progenitors 1-3) with unique transcriptional profiles and cell division kinetics. All progenitor subgroups expressed stemness-related genes such as ANLN, AURKB, and HMGB2 and were detected at all stages of the cell cycle. Notably, Progenitor3 exhibited the highest levels of genes associated with stemness and the G2/M checkpoint, including ANLN, PLK1, AURKA, HMGB2, and TOP2A, and had the largest proportion of cells in G2/M. Progenitor2 was marked by histone H1 expression, while Progenitor1 displayed distinctive cell cycle kinetics. Despite stable proportions of the three progenitor populations throughout prolonged passaging, mitochondrial gene downregulation, and ribosomal gene upregulation were observed. Treatment with the small molecule RepSox partially preserved LSC maintenance in long-term culture by inhibiting the epithelial-mesenchymal transition program and modulating energy and metabolic pathways. These findings provide insights for optimizing in vitro LSC expansion for cell-based therapies.","PeriodicalId":231,"journal":{"name":"STEM CELLS","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12463447/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144504333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Erythropoietin-dependent Acquisition of CD71hiCD105hi Phenotype within CD235a- Early Erythroid Progenitors. CD235a-早期红系祖细胞中CD71hiCD105hi表型的促红细胞生成素依赖性获得

IF 3.6 2区医学

STEM CELLS Pub Date : 2025-09-16 DOI: 10.1093/stmcls/sxaf061

Natascha Schippel, Mrinalini Kala, Shalini Sharma

{"title":"Erythropoietin-dependent Acquisition of CD71hiCD105hi Phenotype within CD235a- Early Erythroid Progenitors.","authors":"Natascha Schippel, Mrinalini Kala, Shalini Sharma","doi":"10.1093/stmcls/sxaf061","DOIUrl":"10.1093/stmcls/sxaf061","url":null,"abstract":"The development of committed erythroid progenitors and their continued maturation into erythrocytes requires the cytokine erythropoietin (Epo). Here, we describe the immunophenotypic identification of a CD34- colony-forming unit-erythroid (CFU-E) progenitor subtype, termed late CFU-E (lateC), that arises in an Epo-dependent manner during human early erythropoiesis (EE). LateC cells lack CD235a (glycophorin A) but have high levels of CD71 and CD105, characterized as Lin-CD123-CD235a-CD49d+CD117+CD34-CD71hiCD105hi. Analysis of ex vivo cultures of bone marrow (BM) CD34+ cells showed that acquisition of the CD71hiCD105hi phenotype in lateC occurs through the formation of four other EE subtypes. Of these, two are CD34+ burst-forming unit-erythroid (BFU-E) cells, distinguishable as CD71loCD105lo early BFU-E (earlyB) and CD71hiCD105lo late BFU-E (lateB), and two are CD34- CFU-E, also distinguishable as CD71loCD105lo early CFU-E (earlyC) and CD71hiCD105lo mid CFU-E (midC). The EE transitions are accompanied by a rise in CD36 expression, such that all lateC cells are immunophenotypically CD36+. Patterns of CD34, CD36, and CD71 indicate two differentiation routes-in one earlyB lose CD34 to form earlyC, and in another, earlyB gain CD36 and CD71hi expression prior to losing CD34 to form midC, bypassing the earlyC stage. Regardless of the route, the transition from midC to lateC requires Epo. All five EE subtypes could be prospectively detected in human BM cells and, upon isolation and reculture, exhibited the potential to continue differentiating along the erythroid trajectory. Finally, we find that all five EE populations can also be detected in cultures of cord blood-derived CD34+ cells at levels similar to those observed in BM CD34+ cell cultures.","PeriodicalId":231,"journal":{"name":"STEM CELLS","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2025-09-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12622993/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145090813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0