IF 3.6 2区医学

STEM CELLS Pub Date : 2026-05-08 DOI: 10.1093/stmcls/sxag028

Anna Pulawska-Czub, Alicja Olczak-Cossu, Tomasz D Pieczonka, Krzysztof Kobielak

{"title":"Nail Proximal Fold Stem Cells (NPFSCs) Participate In Nail Growth, Orchestrating Enhanced Digit Regeneration via BMP Signaling Activation.","authors":"Anna Pulawska-Czub, Alicja Olczak-Cossu, Tomasz D Pieczonka, Krzysztof Kobielak","doi":"10.1093/stmcls/sxag028","DOIUrl":"https://doi.org/10.1093/stmcls/sxag028","url":null,"abstract":"Rodent and primate digit tips exhibit a remarkable regenerative capacity following amputation, driven by highly proliferative nail stem cells (NSCs) with active canonical Wnt signaling. Recently, a distinct, slow-cycling population of bi-functional nail proximal fold stem cells (NPFSCs) has been identified, contributing to both peri-nail epidermis and nail plate (NP). Here, we demonstrate that NPFSCs actively participate in nail growth, orchestrating digit regeneration, with BMP signaling serving as a key regulator. Inhibition of BMP resulted in an epidermalized NP-like structure with limited regeneration due to impaired Wnt pathway activation in the nail matrix cells. Conversely, BMP activation enhanced NPFSCs' involvement in the nail matrix and significantly promoted digit regeneration. We further revealed that enhanced BMP activity not only accelerated nail and bone regrowth but also extended the regenerative boundary proximally, enabling full regeneration after up to ∼60% removal of the distal phalanx (P3). Moreover, in BMP gain-of-function (GoF) models, extreme proximal amputation, removing the majority of the P3, still permitted partial NP regeneration despite the absence of bone reconstruction. Finally, we isolated and cultured lineage-traced NPFSCs and transplanted them into immunocompromised mice, where they integrated into the nail proximal fold and contributed to nail matrix progenitors during regeneration. Transplanted NPFSCs retained their regenerative capacity in vivo, highlighting their therapeutic potential. Collectively, our findings identify a pivotal role of BMP signaling in mediating NPFSC-driven digit regeneration, reveal BMP-Wnt cross-talk as essential to this process, and provide a framework for enhancing regenerative outcomes in previously non-regenerative contexts following traumatic amputation.","PeriodicalId":231,"journal":{"name":"STEM CELLS","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147855522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mesenchymal Stem Cell-Based Delivery Systems for the Treatment of Brain Tumors. 基于间充质干细胞的输送系统治疗脑肿瘤。

IF 3.6 2区医学

STEM CELLS Pub Date : 2026-05-07 DOI: 10.1093/stmcls/sxag024

Mohammad Amin Bakhshan, Leila Chodari, Sayyed Jafar Hasani, Mehran Molavand, Zahra Niknam

{"title":"Mesenchymal Stem Cell-Based Delivery Systems for the Treatment of Brain Tumors.","authors":"Mohammad Amin Bakhshan, Leila Chodari, Sayyed Jafar Hasani, Mehran Molavand, Zahra Niknam","doi":"10.1093/stmcls/sxag024","DOIUrl":"https://doi.org/10.1093/stmcls/sxag024","url":null,"abstract":"Brain tumors rank among the most lethal cancer types, accounting for high mortality rates in both pediatric and adult populations. They present formidable therapeutic obstacles owing to their intrinsically aggressive biology, the complexity of their surrounding microenvironment, and the relatively poor efficacy of existing standard treatment modalities. Mesenchymal stem cells (MSCs) have garnered significant interest in oncology research due to their inherent ability to migrate selectively toward tumor sites and their versatility as delivery vehicles for a wide range of therapeutic agents. This review highlights the diverse therapeutic roles of MSCs and their derivatives, such as exosomes and engineered vectors, in brain tumor management. It encompasses key therapeutic strategies leveraging MSCs, including MSC-mediated delivery of chemotherapeutic agents, oncolytic viruses, and therapeutic genes; exosome-based interventions; nanotechnology-enhanced targeting precision; and the modulation of tumor behavior by engineered MSC-derived cytokines. Despite their promise, concerns remain regarding the potential for MSCs to promote tumor growth and the need for precise delivery systems. This review highlights the crucial need for additional research to refine MSC-based strategies, particularly through the rational engineering of MSCs and their derivatives for multimodal therapeutic applications, with the goal of enhancing specificity, efficacy, and safety in the treatment of malignant brain tumors.","PeriodicalId":231,"journal":{"name":"STEM CELLS","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147831663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of hiPSCs for tissue modeling and therapy: are we on track? hipsc在组织建模和治疗中的应用：我们在轨道上吗？

IF 3.6 2区医学

STEM CELLS Pub Date : 2026-05-07 DOI: 10.1093/stmcls/sxag027

Mathew Nickel Maunu, Ingrid Meulenbelt, Yolande F M Ramos

{"title":"Application of hiPSCs for tissue modeling and therapy: are we on track?","authors":"Mathew Nickel Maunu, Ingrid Meulenbelt, Yolande F M Ramos","doi":"10.1093/stmcls/sxag027","DOIUrl":"https://doi.org/10.1093/stmcls/sxag027","url":null,"abstract":"Stem cells are key for development of disease modeling and therapies. While promising, however, current application of cutting-edge hiPSC technologies is, among others, confounded by cellular heterogeneity leading to concerns about their suitability for experimental and clinical applications. Variations across donors, tissue sources, methodologies, and analytical challenges, together contribute to the observed heterogeneity. Hence, increased understanding of heterogeneity in stem cell research is essential to advance development of reliable tissue models and effective therapies. In this review, we summarize current knowledge regarding the origins of cellular heterogeneity in hiPSC-derivatives. Differentiation protocols can be improved through the application of novel media morphogens, integration with new biomaterials and physical strategies (e.g. 3D culture, mechanical stimulation). Additionally, standardization of methods and regulations for generation and application of cell lines and neo-tissues, thorough characterization, central banking, and registration of cells will reduce variation and increase experimental reproducibility. As reliable reference datasets become more abundant the continuous development of analytical tools as well as advanced application of artificial intelligence to analyze -omics datasets will become more refined. This will aid identification of different cell types in heterogeneous cell populations and key factors driving off-target differentiation. We provide recommendations for best practices throughout the stem cell research pipeline and discuss opportunities to advance broad applicability of stem cells for disease modeling and beyond through concerted efforts to improve experimental robustness and analytical accuracy. Finally, we advocate that certain heterogeneity may be essential in development of laboratory models to faithfully mimic the in vivo situation.","PeriodicalId":231,"journal":{"name":"STEM CELLS","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-05-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147831691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Huntington's Disease Human Lateral Ganglionic Eminence Precursors Differentiate into Functionally Mature Medium Spiny Neurons Exhibiting Pathology. 亨廷顿氏病人类外侧神经节隆起前体细胞分化为功能成熟的中棘神经元，显示病理。

IF 3.6 2区医学

STEM CELLS Pub Date : 2026-05-06 DOI: 10.1093/stmcls/sxag025

Amy McCaughey-Chapman, Bronwen Connor

{"title":"Huntington's Disease Human Lateral Ganglionic Eminence Precursors Differentiate into Functionally Mature Medium Spiny Neurons Exhibiting Pathology.","authors":"Amy McCaughey-Chapman, Bronwen Connor","doi":"10.1093/stmcls/sxag025","DOIUrl":"https://doi.org/10.1093/stmcls/sxag025","url":null,"abstract":"Huntington's disease (HD) is an autosomal dominant neurodegenerative disease characterised by the loss of GABAergic medium spiny neurons (MSNs). Cellular models of HD are mainly derived from human embryonic stem cells or induced pluripotent stem cells. These models are limited by their DNA embryonic age, low neuronal yields and limited disease pathology. We propose direct reprogramming, which maintains the aging signature of the cells, to human induced lateral ganglionic eminence precursors (hiLGEP) results in the generation of high yields of functionally mature MSNs exhibiting pathological hallmarks of HD. hiLGEPs were derived from normal and HD fibroblasts by direct reprogramming and differentiated to MSNs. hiLGEP and MSN fate acquisition was compared between normal and HD through gene and protein expression. Known pathological hallmarks of HD were investigated within the hiLGEP-derived MSNs. The formation of functional synapses was investigated using live cell calcium imaging. We demonstrate that HD fibroblasts can be reprogrammed to hiLGEPs expressing key linage markers and displaying disease-related changes in expression of FOXP1 and FOXP2. HD hiLGEPs can be differentiated to high yields of MSNs co-expressing DARPP32, GABA, or GAD65/67, and SYN1 and PSD-95. HD MSNs show a reduced expression of BDNF, HAP1, TRKB, Rhes and PGC1α, exhibit MW8+ mHTT aggregates and display smaller cell somas, reduced total neurite length and reduced branched neurites when compared to normal MSNs. An administration of 100 µM dopamine was necessary to generate a calcium response in HD MSNs. This study establishes a directly reprogrammed hiLGEP-derived MSN model of HD which recapitulates pathological signatures.","PeriodicalId":231,"journal":{"name":"STEM CELLS","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147831739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transplantation of human stem cell-derived cone photoreceptors partially restores vision in aged rd1 mice with advanced retinal degeneration. 人类干细胞来源的视锥光感受器移植可部分恢复老年rd1小鼠的视力。

IF 3.6 2区医学

STEM CELLS Pub Date : 2026-05-02 DOI: 10.1093/stmcls/sxag023

Christopher A Procyk, Anna Melati, Menahil Tariq, Jingshu Liu, Matthew J Branch, Jamie D Delicata, Philippa Harding, Mahmoud Khazim, Majid Moshtagh Khorasani, Bryan Ladino, Emily P Lanning, Miriam Margari, Ifrax Mahamoud, Christi Mofidi, Krunal Narendra Kumar, Salome Van Heerden, Alexander J Smith, Emma L West, Robin R Ali, Rachael A Pearson

{"title":"Transplantation of human stem cell-derived cone photoreceptors partially restores vision in aged rd1 mice with advanced retinal degeneration.","authors":"Christopher A Procyk, Anna Melati, Menahil Tariq, Jingshu Liu, Matthew J Branch, Jamie D Delicata, Philippa Harding, Mahmoud Khazim, Majid Moshtagh Khorasani, Bryan Ladino, Emily P Lanning, Miriam Margari, Ifrax Mahamoud, Christi Mofidi, Krunal Narendra Kumar, Salome Van Heerden, Alexander J Smith, Emma L West, Robin R Ali, Rachael A Pearson","doi":"10.1093/stmcls/sxag023","DOIUrl":"https://doi.org/10.1093/stmcls/sxag023","url":null,"abstract":"Targeted photoreceptor replacement therapy is a promising, potentially disease-agnostic approach for reversing sight-loss associated with advanced retinal degenerations, including age-related macular degeneration. We have previously shown that transplantation of human stem cell-derived cone photoreceptors (hCones) into young adult (3-month-old) mouse models of advanced retinal degeneration can restore retinal function. However, substantial remodeling of the remaining inner retinal circuitry continues long after complete photoreceptor loss, raising the critical question of whether photoreceptor transplantation can effectively rescue function at very late-stage degeneration. rd1 mice received transplants at 12-15 months of age and were examined ∼3 months later. Transplanted hCones survived in large numbers, while host inner retinal neurons exhibited significant plasticity, extending dendrites to transplanted hCones, and making synapse-like contacts. Host Müller glia undergo notable remodeling, apparently incorporating the donor cells within the retinal structure. Multielectrode array recordings showed robust rescue of light-evoked activity across the normal photopic range intensities and evidence of inner retinal processing, while some treated mice showed improvements in visually-evoked optokinetic head tracking behavior. Together, these data indicate that effective rescue following photoreceptor replacement therapy is feasible long after complete photoreceptor loss and extensive inner retinal remodeling.","PeriodicalId":231,"journal":{"name":"STEM CELLS","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147831711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prostaglandin E2 receptor EP2 is indispensable for maintenance of skeletal muscle stem cells. 前列腺素E2受体EP2对骨骼肌干细胞的维持是不可缺少的。

IF 3.6 2区医学

STEM CELLS Pub Date : 2026-04-25 DOI: 10.1093/stmcls/sxag012

Yusuke Maruyama, Ken'ichiro Nogami, Norio Motohashi, Fusako Sakai-Takemura, Ahmed Elhussieny, Fumiaki Uchiumi, Yoshitsugu Aoki, Shin'ichi Takeda, Yuko Miyagoe-Suzuki

{"title":"Prostaglandin E2 receptor EP2 is indispensable for maintenance of skeletal muscle stem cells.","authors":"Yusuke Maruyama, Ken'ichiro Nogami, Norio Motohashi, Fusako Sakai-Takemura, Ahmed Elhussieny, Fumiaki Uchiumi, Yoshitsugu Aoki, Shin'ichi Takeda, Yuko Miyagoe-Suzuki","doi":"10.1093/stmcls/sxag012","DOIUrl":"10.1093/stmcls/sxag012","url":null,"abstract":"Muscle satellite cells are adult muscle stem cells indispensable for growth and regeneration of postnatal skeletal muscle. Notch plays a central role in maintenance of muscle satellite cells, but how Notch maintains the muscle stem cell pool is not fully understood. Previously, we reported that a prostaglandin E2 receptor, EP2, is upregulated by Notch signal and suppresses differentiation of human muscle progenitors. Here we examined the roles of EP2 in muscle satellite cells using a mouse Cre-LoxP conditional gene knockout system. Genetic inactivation of the EP2 gene (PTGER2) activated muscle satellite cells, caused their loss, and impaired muscle regeneration. These results indicate that EP2 is indispensable for maintenance of satellite cells. Ex vivo analysis using isolated myofibers showed that prostaglandin E2 (PGE2) delayed the activation of satellite cells via EP2. An extracellular signal-regulated kinase (ERK) 1/2 inhibitor blocked the activation of satellite cells on myofibers, and PGE2 attenuated the phosphorylation of ERK1/2 in muscle satellite cells. These results suggest that EP2 keeps the quiescence of satellite cells and maintains the satellite cell pool in part by inhibiting the ERK1/2 signaling pathway.","PeriodicalId":231,"journal":{"name":"STEM CELLS","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13110117/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147389022","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Highly efficient induction of Sox9+ scl-progenitors from embryonic stem cells for skeletal modeling. 从胚胎干细胞高效诱导Sox9+ scl祖细胞用于骨骼建模。

IF 3.6 2区医学

STEM CELLS Pub Date : 2026-04-25 DOI: 10.1093/stmcls/sxag007

Yanhui Deng, Jingfei Xiong, Hanyi Chen, Runxin Ma, Weidong Tian, Yike Yin, Zhonghan Li

{"title":"Highly efficient induction of Sox9+ scl-progenitors from embryonic stem cells for skeletal modeling.","authors":"Yanhui Deng, Jingfei Xiong, Hanyi Chen, Runxin Ma, Weidong Tian, Yike Yin, Zhonghan Li","doi":"10.1093/stmcls/sxag007","DOIUrl":"10.1093/stmcls/sxag007","url":null,"abstract":"Sclerotomal progenitors derived from pluripotent stem cells hold promises for modeling skeletal development and recapitulating the perinatal marrow niche that may provide insights into hematopoietic niche formation and immune regulation. Current strategy to derive mouse sclerotomal progenitors suffered from low differentiation efficiency and heterogeneous cell progenies. Here, we developed a fast and efficient strategy to generate sclerotomal progenitors by accelerated induction from primitive streak (PS) through modulating BMP and SHH signaling, achieving an 86.9% differentiation efficiency. Moreover, the resulting progenitors showed similar global gene expression profiles to those of the primary sclerotome, possessed strong osteochondral bipotential, and could recapitulate key features of endochondral ossification upon micromass-mediated osteogenic induction, including perinatal bone marrow (BM)-like niches regeneration. Our findings underscore accelerated sclerotomal induction from the primitive streak as an efficient strategy to derive sclerotomal progenitors for skeletal modeling and BM niche bioengineering.","PeriodicalId":231,"journal":{"name":"STEM CELLS","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146211774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Extracellular vesicle microRNAs from human bone marrow MSCs suppress fibrogenesis of hepatic stellate cells by downregulation of RhoA signaling. 人骨髓间充质干细胞细胞外囊泡microrna通过下调RhoA信号通路抑制肝星状细胞的纤维化。

IF 3.6 2区医学

STEM CELLS Pub Date : 2026-04-25 DOI: 10.1093/stmcls/sxag006

Daiki Kawamoto, Toshihiko Matsumoto, Tsuyoshi Fujioka, Shuhei Shinoda, Koichi Fujisawa, Tsuyoshi Ishikawa, Naoki Yamamoto, Taro Takami

{"title":"Extracellular vesicle microRNAs from human bone marrow MSCs suppress fibrogenesis of hepatic stellate cells by downregulation of RhoA signaling.","authors":"Daiki Kawamoto, Toshihiko Matsumoto, Tsuyoshi Fujioka, Shuhei Shinoda, Koichi Fujisawa, Tsuyoshi Ishikawa, Naoki Yamamoto, Taro Takami","doi":"10.1093/stmcls/sxag006","DOIUrl":"10.1093/stmcls/sxag006","url":null,"abstract":"We have developed a liver regeneration therapy for decompensated liver cirrhosis (DLC) using autologous bone marrow mesenchymal stem cells (MSCs), and it is currently under trial (jRCT2063200014). However, the mechanism underlying the anti-fibrotic action of MSCs remains unclear. Therefore, we aimed to investigate the anti-fibrotic effects of microRNAs (miRNAs) in extracellular vesicles (EVs) derived from human bone marrow MSCs. We performed a comprehensive microarray analysis of miRNA expression profiles of MSC-EVs from healthy individuals, patients with DLC, and hepatic stellate cells (HSCs). We identified 11 miRNAs that showed a normalized intensity > 1000 in MSC-EVs, which was 50-fold higher than that in HSCs. MiRNA mimics for the 11 miRNAs were transfected into HSCs, and five miRNAs (miR-204-3p, miR-7977, miR-1237-5p, miR-5787, and miR-6089) that suppressed the expression of fibrosis-related genes (α-SMA, collagen I, and elastin) were identified. Transfection of the five-miRNA cocktail into HSCs decreased the expression of all fibrosis-related genes. Subsequently, pathway analysis revealed the downregulation of the RhoA signaling pathway, a key pathway in HSC activation. RhoA activity and migration were suppressed in HSCs transfected with the five-miRNA cocktail. This study identified five anti-fibrotic miRNAs enriched in MSC-EVs and elucidated the underlying mechanism of the anti-fibrotic action of MSCs.","PeriodicalId":231,"journal":{"name":"STEM CELLS","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146211801","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Age-dependent role of histone deacetylase Sirt7 on haematopoiesis. 组蛋白去乙酰化酶Sirt7在造血中的年龄依赖性作用。

IF 3.6 2区医学

STEM CELLS Pub Date : 2026-04-25 DOI: 10.1093/stmcls/sxag005

Hannah Willems, Reinhard Bauer, Jörg P Müller

引用次数: 0

Mesenchymal stem cells derived EXO-miR3671 delivery promoted angiogenesis and accelerated wound healing of diabetes. 间充质干细胞来源的EXO-miR3671递送促进血管生成和加速糖尿病伤口愈合。

IF 3.6 2区医学

STEM CELLS Pub Date : 2026-04-25 DOI: 10.1093/stmcls/sxag008

Jiannan Li, Sibing Chen, Huannan Wang, Wan Wang, Yanxi Liu

{"title":"Mesenchymal stem cells derived EXO-miR3671 delivery promoted angiogenesis and accelerated wound healing of diabetes.","authors":"Jiannan Li, Sibing Chen, Huannan Wang, Wan Wang, Yanxi Liu","doi":"10.1093/stmcls/sxag008","DOIUrl":"10.1093/stmcls/sxag008","url":null,"abstract":"Objective: To explore the effect of exosomes-encapsulated miR3671 (EXO-miR3671) from mesenchymal stem cells (MSC) on diabetic wound healing and its mechanism.Methods: Bioinformatics analysis was conducted to identify gene and miRNA expression changes associated with diabetic foot ulcer (DFU). A diabetic mouse wound model was established, and dual-luciferase reporter assay was performed to verify the targeting relationship between miR3671 and QSOX1. For in vitro experiments, human umbilical vein endothelial cells (HUVECs) were cultured and transfected; the effects of EXO-miR3671 secreted by mesenchymal stem cells on HUVEC biological functions were detected via EdU proliferation assay, Transwell migration assay, and angiogenesis assay. For in vivo experiments, after dynamic measurement of wound areas in diabetic mice, HE staining, immunohistochemistry, and Western blot were employed to evaluate the regulatory effects of mesenchymal stem cell-derived EXO-miR3671 on wound healing process and related protein expression levels.Results: MiR3671 was downregulated in DFU tissues and diabetic mouse wounds. EXO-miR3671 promoted HUVEC proliferation, migration, and angiogenesis in vitro. In diabetic mice, it accelerated wound closure, reduced inflammation, increased Ki67+ cell proliferation, and upregulated pro-angiogenic growth factors.Conclusions: MSC-derived EXO-miR3671 could be a potential treatment for diabetic wounds, providing new insights for treatment.","PeriodicalId":231,"journal":{"name":"STEM CELLS","volume":" ","pages":""},"PeriodicalIF":3.6,"publicationDate":"2026-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146211799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

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