Tissue engineering and regenerative medicine最新文献

{"title":"Corneal Endothelium Regeneration with Decellularized Porcine Corneal Extracellular Matrix Scaffolds.","authors":"Cha Yeon Kim, Cholong Jeong, Hun Lee, Changmo Hwang","doi":"10.1007/s13770-025-00734-9","DOIUrl":"10.1007/s13770-025-00734-9","url":null,"abstract":"<p><strong>Background: </strong>To evaluate the structural, biochemical, and functional performance of decellularized porcine corneal extracellular matrix (dECM) scaffolds for engineering human corneal endothelium.</p><p><strong>Methods: </strong>Porcine corneas were decellularized using either 0.3% sodium dodecyl sulfate (SDS) or 1.5 M sodium chloride (NaCl), followed by enzymatic nucleic acid digestion. Histological and biochemical analyses were performed to assess decellularization efficiency and extracellular matrix preservation. Human corneal endothelial cells (hCECs) were cultured on SDS-dECM scaffolds to evaluate cytocompatibility, morphology, and functional outcomes. Therapeutic efficacy was further assessed using a rabbit model of corneal endothelial dystrophy (CED).</p><p><strong>Results: </strong>SDS-treated corneas showed superior nuclear clearance (residual DNA: 123.60 ± 8.92 ng/mg) compared to NaCl (146.15 ± 5.49 ng/mg), with 95.2% retention of sulfated glycosaminoglycans (sGAGs) and moderate collagen loss (40% of native). In contrast, NaCl better preserved collagen (100% of native) but exhibited incomplete decellularization and lower sGAG retention (71.0%). In vitro, hCECs cultured on SDS-dECM exhibited progressive proliferation, with cell viability surpassing that of TCPS by day 14 (389.01 ± 5.68 vs. 359.65 ± 7.92, p < 0.05). Immunofluorescence confirmed polygonal morphology and ZO-1 expression, indicating intact barrier phenotype. Transparency of dECM scaffolds improved with hCEC culture, with light transmittance at 400 nm increasing from 65.82% (acellular) to 90.13% (double-sided culture). In vivo transplantation of hCEC-seeded SDS-dECM resulted in dose-dependent corneal clarity restoration, with the high-dose group achieving transparency and pachymetry comparable to normal corneas (thickness ~ 602 µm, grading score 0.00 ± 0.00) by 16 weeks.</p><p><strong>Conclusions: </strong>SDS-dECM scaffolds demonstrated excellent biocompatibility and functional support for human corneal endothelial cells, both in vitro and in vivo. These findings support their potential use as bioengineered alternatives to donor corneas for treating endothelial dysfunction.</p>","PeriodicalId":23126,"journal":{"name":"Tissue engineering and regenerative medicine","volume":" ","pages":"735-746"},"PeriodicalIF":4.4,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12209104/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144326879","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exosomes of Human Fetal Cartilage Progenitor Cells (hFCPCs) Inhibited Interleukin-1β (IL-1β)-Induced Osteoarthritis Phenotype via miR-125b-5p In Vitro.","authors":"JuHyeok Lee, Jiyoung Lee, Byung Hyune Choi","doi":"10.1007/s13770-025-00720-1","DOIUrl":"10.1007/s13770-025-00720-1","url":null,"abstract":"<p><strong>Background: </strong>This study investigated anti-inflammatory effects of exosomes derived from human fetal cartilage progenitor cells (hFCPC-Exo) and their microRNAs (miRNAs) on the osteoarthritis (OA) phenotype in vitro in comparison with exosomes from bone marrow mesenchymal stem cells (MSC-Exo).</p><p><strong>Methods: </strong>SW982 cells (synoviocytes) or hFCPCs (chondrocytes) were stimulated with 10 ng/mL IL-1β to mimic OA phenotypes. The effects of hFCPC-Exo and MSC-Exo were compared by measuring the expression of inflammatory cytokines and an anti-inflammatory protein. miRNA profiles of hFCPC-Exo and MSC-Exo were analyzed using a 2588 human miRNA dataset, and miRNAs potentially involved in the anti-inflammatory effect of hFCPC-Exo were selected. miRNA mimics and antisense inhibitors were used to investigate the role of selected miRNAs in the IL-1β signaling pathways.</p><p><strong>Results: </strong>Both hFCPC-Exo and MSC-Exo significantly decreased the expression of inflammatory cytokines (IL-1β, IL-6, and MCP-1), while slightly increased an anti-inflammatory protein (SOCS1) in IL-1β-treated SW982 cells. miRNA sequencing revealed anti-inflammatory miRNAs present in large amounts in both hFCPC-Exo and MSC-Exo. Among them, miR-125b-5p mimic significantly suppressed the expression of inflammatory cytokines induced by IL-1β, while anti-sense inhibitor of miR-125b-5p efficiently blocked anti-inflammatory effects of hFCPC-Exo. Both hFCPC-Exo and miR-125b-5p inhibited IκBα down-regulation and -NF-κB stabilization in IL-1β-treated SW982 cells. Additionally, hFCPC-Exo and miR-125b-5p showed similar effects on IL-1β-treated hFCPCs as an OA model in chondrocytes by down-regulating the expression of IL-1β, MMP13, and ADAMTS-5 and up-regulating the expression of aggrecan (ACAN) and type II collagen (COL2A1).</p><p><strong>Conclusion: </strong>This study demonstrated that hFCPC-Exo exhibits anti-inflammatory effects on IL-1β-treated synoviocytes and chondrocytes in vitro possibly by down-regulating the IL-1β-TRAF6-NF-κB pathway via anti-inflammatory miRNAs such as miR-125b-5p.</p>","PeriodicalId":23126,"journal":{"name":"Tissue engineering and regenerative medicine","volume":" ","pages":"691-703"},"PeriodicalIF":4.1,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12209160/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144080432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Construction of Chitosan Oligosaccharide-Coated Nanostructured Lipid Carriers for the Sustained Release of Strontium Ranelate.","authors":"Hayeon Lim, Yoseph Seo, Sung Jun Min, Daehyeon Yoo, Dong Nyoung Heo, Il Keun Kwon, Taek Lee","doi":"10.1007/s13770-025-00713-0","DOIUrl":"10.1007/s13770-025-00713-0","url":null,"abstract":"<p><strong>Background: </strong>Strontium ranelate (SR) is an effective bone regeneration drug; however, its low bioavailability and strong hydrophilicity cause a strong cytotoxicity, venous thrombosis, and allergic reactions when administered in its free form. This study aims to enhance the SR bioavailability by utilizing nanostructured lipid carriers (NLC) as a drug delivery system (DDS).</p><p><strong>Methods: </strong>To improve the drug delivery efficiency and sustained release of the NLC, their surfaces were coated with chitosan oligosaccharide (COS), a natural polymer. The synthesis of COS-NLC was confirmed by measuring particle size and zeta potential, while surface morphology was evaluated using atomic force microscopy (AFM). SR loading efficiencies and release profiles were analyzed via reversed-phase high-performance liquid chromatography (RP-HPLC), and cytotoxicity was evaluated in mouse fibroblast L929 cells.</p><p><strong>Results: </strong>Particle characterization indicated that the COS coating slightly increased the particle size (i.e., from 128.99 ± 2.77 to 131.46 ± 2.13 nm) and zeta potential (i.e., from - 13.94 ± 0.49 to - 6.58 ± 0.32 mV) of the NLC. The COS-NLC exhibited a high SR-loading efficiency of ~ 86.31 ± 3.28%. An in vitro release test demonstrated an improved sustained release tendency of SR from the COS-NLC compared to that from the uncoated NLC. In cytotoxicity assays using L929 cells, the COS coating reduced the cytotoxicity of the formulated DDS, and the SR-COS-NLC exhibited a 1.4-fold higher cell regeneration effect than SR alone.</p><p><strong>Conclusion: </strong>These findings suggest that the developed COS-NLC serve as an effective and biocompatible DDS platform for the delivery of poorly bioavailable drugs.</p>","PeriodicalId":23126,"journal":{"name":"Tissue engineering and regenerative medicine","volume":" ","pages":"425-440"},"PeriodicalIF":4.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12122978/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143617195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis of Autotaxin-Inhibiting Lipid Nanoparticles to Regulate Autophagy and Inflammatory Responses in Activated Macrophages.","authors":"So Won Jeon, Jun Kwon, Hee Gyeong Ko, Jong Sang Yoon, Yun A Kim, Ju-Ro Lee, Min-Ho Kang, Han Young Kim","doi":"10.1007/s13770-025-00705-0","DOIUrl":"10.1007/s13770-025-00705-0","url":null,"abstract":"<p><strong>Background: </strong>Autotaxin (ATX), an ENPP2 enzyme, regulates lipid signaling by converting lysophosphatidylcholine to lysophosphatidic acid (LPA). Dysregulation of the ATX/LPA axis promotes inflammation and disease progression. BMP-22, a lipid ATX inhibitor, effectively reduces LPA production. However, its clinical utility is hampered by limitations in solubility and pharmacokinetics. To overcome these limitations, we developed BMP-22-incorporated lipid nanoparticles (LNP-BMP) to improve utility while maintaining ATX inhibition efficacy.</p><p><strong>Methods: </strong>LNP-BMP was synthesized by incorporating DOTAP, DOPE, cholesterol, 18:0 PEG₂₀₀₀-PE, and together with BMP-22. The formulation of LNP-BMP was optimized and characterized by testing different molar ratios of BMP-22. The autophagy recovery and anti-inflammatory effects of LNP-BMP via ATX inhibition were evaluated in both macrophage cell line and mouse-derived primary macrophages.</p><p><strong>Results: </strong>LNP-BMP was shown to retain its functionality as an ATX inhibitor and maintain the physical characteristics upon BMP-22 integration. Synthesized LNP-BMP exerted superior ability to inhibit ATX activity. When applied to M1-induced macrophages, LNP-BMP exhibited substantial anti-inflammatory effects and successfully restored autophagy activity.</p><p><strong>Conclusion: </strong>The results demonstrate that LNP-BMP effectively inhibits ATX, achieving both anti-inflammatory effects and autophagy restoration, highlighting its potential as a standalone immunotherapeutic agent. Furthermore, the capacity to load therapeutic drugs into this formulation offers promising opportunities for further therapeutic strategies.</p>","PeriodicalId":23126,"journal":{"name":"Tissue engineering and regenerative medicine","volume":" ","pages":"397-408"},"PeriodicalIF":4.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12122970/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143493534","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Innovations in Vascular Repair from Mechanical Intervention to Regenerative Therapies.","authors":"Hye-Min Park, Chae-Lin Kim, Dasom Kong, Seon-Hee Heo, Hyun-Ji Park","doi":"10.1007/s13770-024-00700-x","DOIUrl":"10.1007/s13770-024-00700-x","url":null,"abstract":"<p><strong>Background: </strong>Vascular diseases, including atherosclerosis and thrombosis, are leading causes of morbidity and mortality worldwide, often resulting in vessel stenosis that impairs blood flow and leads to severe clinical outcomes. Traditional mechanical interventions, such as balloon angioplasty and bare-metal stents, provided initial solutions but were limited by restenosis and thrombosis. The advent of drug-eluting stents improved short-term outcomes by inhibiting vascular smooth muscle cell proliferation, however, they faced challenges including delayed reendothelialization and late-stage thrombosis.</p><p><strong>Methods: </strong>This review highlights the progression from mechanical to biological interventions in treating vascular stenosis and underscores the need for integrated approaches that combine mechanical precision with regenerative therapies.</p><p><strong>Results: </strong>To address long-term complications, bioresorbable stents were developed to provide temporary scaffolding that gradually dissolves, yet they still encounter challenges with mechanical integrity and optimal degradation rates. Consequently, emerging therapies now focus on biological approaches, such as gene therapy, extracellular vesicle treatments, and cell therapies, that aim to promote vascular repair at the cellular level. These strategies offer the potential for true vascular regeneration by enhancing endothelialization, modulating immune responses, and stimulating angiogenesis.</p><p><strong>Conclusion: </strong>Integrating mechanical precision with regenerative biological therapies offers a promising future for treating vascular stenosis. A comprehensive approach combining these modalities could achieve sustainable vascular health.</p>","PeriodicalId":23126,"journal":{"name":"Tissue engineering and regenerative medicine","volume":" ","pages":"551-567"},"PeriodicalIF":4.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12122965/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143374519","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antioxidant Peptide-Based Nanocarriers for Delivering Wound Healing Agents.","authors":"Inseo Lee, Woo Hyun Kwon, Joo-Young Kim, Ha Kyeong Kim, Ji-Eun Kim, Yong-Beom Lim, Woo-Jin Jeong, Jun Shik Choi","doi":"10.1007/s13770-025-00701-4","DOIUrl":"10.1007/s13770-025-00701-4","url":null,"abstract":"<p><strong>Background: </strong>Curcumin, a well-known wound healing agent, faces clinical limitations due to its poor water solubility, rapid degradation, and short plasma half-life. To address these challenges, we developed a self-assembling peptide incorporating an antioxidant sequence (YGDEY), which is capable of not only delivering curcumin but also exhibiting additional bioactivity to enhance wound healing.</p><p><strong>Methods: </strong>An antioxidant nanocarrier was developed via peptide self-assembly. To design an amphiphilic peptide for the nanocarrier assembly, antioxidant peptide sequence (YGDEY) as the hydrophilic segment and the hydrophobic block (WLWL) were incorporated to single peptide molecule. The peptide's self-assembly behavior and curcumin encapsulation were initially analyzed. Subsequent evaluations included cytocompatibility, cellular uptake, and antioxidant activity.</p><p><strong>Results: </strong>Driven by strong interactions among their hydrophobic blocks (WLWL), the peptides formed well-defined nanostructures exhibiting high thermal stability. Furthermore, the encapsulation of curcumin within the micelle significantly improved its cellular penetration efficiency. When applied to fibroblast cells, the peptide-curcumin nanocomplexes exhibited synergistically enhanced antioxidant activity, which notably outperformed free curcumin and free peptide in scavenging reactive oxygen species.</p><p><strong>Conclusion: </strong>These findings highlight the potential of the designed peptide-based nanocarrier to overcome intrinsic limitations of curcumin and enhance its therapeutic efficacy, providing a promising strategy for advanced wound healing applications.</p>","PeriodicalId":23126,"journal":{"name":"Tissue engineering and regenerative medicine","volume":" ","pages":"441-451"},"PeriodicalIF":4.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12122397/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143383361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polynucleotide and Hyaluronic Acid Mixture for Skin Wound Dressing for Accelerated Wound Healing.","authors":"Tae-Hyun Heo, Bon Kang Gu, Kyungeun Ohk, Jeong-Kee Yoon, Young Hoon Son, Heung Jae Chun, Dae-Hyeok Yang, Gun-Jae Jeong","doi":"10.1007/s13770-025-00712-1","DOIUrl":"10.1007/s13770-025-00712-1","url":null,"abstract":"<p><strong>Background: </strong>Skin wound healing is a complex process requiring coordinated cellular and molecular interactions. Polynucleotides (PN) and hyaluronic acid (HA) have emerged as promising agents in regenerative medicine due to their ability to enhance cellular proliferation, angiogenesis, and extracellular matrix (ECM) remodeling. Combining PN and HA offers potential synergistic effects, accelerating wound repair.</p><p><strong>Methods: </strong>PN and HA hydrogels were prepared and evaluated for viscosity and gel stability. Their effects on human dermal fibroblasts (HDF) and keratinocytes (HaCaT) were assessed using migration, proliferation assays, and gene expression analyses for vascular endothelial growth factor (VEGF), matrix metalloproteinase-9 (MMP-9), and matrix metalloproteinase-10 (MMP-10). In vivo studies were conducted using a mouse wound model to observe wound closure and tissue regeneration over 14 days.</p><p><strong>Results: </strong>The PN-HA mixture demonstrated superior mechanical stability compared to individual components. In vitro, PN-HA significantly enhanced HDF and HaCaT migration, proliferation, and upregulated VEGF, MMP-9, and MMP-10 expression. In vivo, PN-HA treatment accelerated wound closure, improved dermal thickness, and enhanced ECM remodeling, as evidenced by histological analyses.</p><p><strong>Conclusion: </strong>The PN-HA combination synergistically accelerates wound healing by promoting angiogenesis, cellular migration, and ECM remodeling. These findings highlight its potential as an advanced wound dressing for acute and chronic wound management.</p>","PeriodicalId":23126,"journal":{"name":"Tissue engineering and regenerative medicine","volume":" ","pages":"515-526"},"PeriodicalIF":4.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12122959/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143504324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Branched Polymer Architecture for Modulating Interactions in Material-Bio Interface.","authors":"Fahimeh Taghavimandi, Min Gyu Kim, Mingyu Lee, Kwangsoo Shin","doi":"10.1007/s13770-024-00699-1","DOIUrl":"10.1007/s13770-024-00699-1","url":null,"abstract":"<p><strong>Background: </strong>Branched polymers, including star, dendrimers, comb, and biomimetic polymers, are increasingly recognized for their potential in tissue engineering. Their unique architectures and functional properties contribute to enhanced mechanical strength, bioactivity, and adaptability of scaffolds and hydrogels.</p><p><strong>Objective: </strong>This review explores the diverse applications of branched polymers in tissue engineering and regenerative medicine, emphasizing their role in mimicking the extracellular matrix (ECM) and modulating interactions at the material-bio interface. The structural features of branched polymers, including branching density and functional group distribution, are highlighted for their influence on drug delivery, mechanical properties, and cellular interactions.</p><p><strong>Results: </strong>Branched polymers offer distinct advantages in tissue engineering: Star polymers: Provide tunable elasticity and facilitate long-range mechanical networking. Dendrimers: Enable precise functionalization for targeted drug delivery and cell signaling. Comb polymers: Enhance porosity and nutrient exchange in scaffolds. Biomimetic polymers: Mimic natural biological systems, promoting cellular adhesion, proliferation, and differentiation.</p><p><strong>Conclusion: </strong>Branched polymers represent a versatile and promising platform for tissue engineering and regenerative medicine. Their ability to modulate biological interactions and adapt to diverse functional requirements underscores their potential to advance the field.</p>","PeriodicalId":23126,"journal":{"name":"Tissue engineering and regenerative medicine","volume":" ","pages":"481-504"},"PeriodicalIF":4.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12122963/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143582344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunomodulation Effects of Porcine Cartilage Acellularized Matrix (pCAM) for Osteoarthritis Treatment.","authors":"Ji Seob Kim, Hyeon Jae Kwon, In Sun Hwang, Young Hwa Lee, Kyung-Noh Yoon, Hee-Woong Yun, Jae-Hyeok Jang, Seo Jeong Kim, Zhoodatova Aiana, Seungwoo Kim, Minhee Moon, Bongki Kim, Byoung Ju Kim, Byung-Hyun Cha","doi":"10.1007/s13770-024-00687-5","DOIUrl":"10.1007/s13770-024-00687-5","url":null,"abstract":"<p><strong>Background: </strong>Pain reduction, immunomodulation, and cartilage repair are key therapeutic goals in osteoarthritis (OA) treatment. In this study, we evaluated the therapeutic effects of porcine cartilage acellularized matrix (pCAM) derived from naive tissue and compared it with the synthetic material polynucleotides (PN) for OA treatment.</p><p><strong>Methods: </strong>pCAM was produced from porcine cartilage through physicochemical processing. LC-MS protein profiling identified the key proteins. In vitro experiments involved treating human synovial cell with pCAM and PN to assess cell viability and reductions in pro-inflammatory cytokines (IL-1β and IL-6). In vivo studies utilized a rat DMM-induced OA model. Pain was evaluated in weight-bearing tests, and inflammation reduction was confirmed using specific macrophage markers of CD68, CD86, and CD163 in immunohistochemical staining of synovial tissue. Cartilage regeneration was evaluated by histopathological analyses.</p><p><strong>Results: </strong>The major protein components of pCAM include factors integral to cartilage and ECM integrity. They also contain proteins that help reduce inflammation. In vitro studies revealed a decrease in pro-inflammatory cytokines and survival of synovial cells were observed. In vivo treatment with pCAM resulted in a reduction of pain and inflammation, while promoting cartilage regeneration, thereby accelerating the healing process in OA.</p><p><strong>Conclusion: </strong>Our findings suggest that pCAM may contribute to the treatment of OA by alleviating synovial inflammation and supporting cartilage regeneration, thereby addressing both the inflammatory and degenerative aspects of the disease.</p>","PeriodicalId":23126,"journal":{"name":"Tissue engineering and regenerative medicine","volume":" ","pages":"453-467"},"PeriodicalIF":4.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12123000/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142955535","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Injectable Endoplasmin-Loaded Lipid Nanoparticles-Hydrogel Composite for Cartilage Regeneration.","authors":"Sumi Choi, Hyeongrok Choi, Jin Woong Chung, Su-Hwan Kim","doi":"10.1007/s13770-024-00698-2","DOIUrl":"10.1007/s13770-024-00698-2","url":null,"abstract":"<p><strong>Background: </strong>Endoplasmin (ENPL), a heat shock protein 90 family member, promotes chondrogenic differentiation of stem cells by inhibiting ERK1/2 phosphorylation and inducing endoplasmic reticulum stress. However, its large size limits cellular uptake and therapeutic potential. To overcome this challenge, a cationic lipid nanoparticle (C_LNP) system was designed to deliver ENPL intracellularly, enhancing its effects on human tonsil-derived mesenchymal stem cells (hTMSCs).</p><p><strong>Methods: </strong>ENPL-loaded cationic lipid nanoparticles (ENPL_C_LNP) were synthesized to facilitate intracellular ENPL delivery. The delivery efficiency and cytotoxicity were assessed in vitro using hTMSCs. Additionally, ENPL_C_LNPs were incorporated into a hyaluronic acid and chondroitin sulfate-based injectable hydrogel and tested for chondrogenic differentiation potential in a mouse subcutaneous model.</p><p><strong>Results: </strong>ENPL_C_LNP achieved over 80% intracellular protein delivery efficiency with no cytotoxic effects. Co-cultured hTMSCs exhibited increased glycosaminoglycans (GAGs) and collagen expression over 21 days. In vivo, the hydrogel-embedded ENPL_C_LNP system enabled stable cartilage differentiation, evidenced by abundant cartilage-specific lacuna structures in regenerated tissue.</p><p><strong>Conclusion: </strong>Combining ENPL_C_LNP with an injectable hydrogel scaffold supports chondrogenic differentiation and cartilage regeneration, offering a promising strategy for cartilage tissue engineering.</p>","PeriodicalId":23126,"journal":{"name":"Tissue engineering and regenerative medicine","volume":" ","pages":"409-424"},"PeriodicalIF":4.4,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12122951/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143483979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}