The Ukrainian Biochemical Journal最新文献

{"title":"Diet-induced and age-related changes in rats: the impact of N-stearoylethanolamine intake on plasma lipoproteins, adiponectin, and adipocyte cholesterol-phospholipid composition","authors":"O. S. Tkachenko, H. Kosiakova","doi":"10.15407/ubj96.02.084","DOIUrl":"https://doi.org/10.15407/ubj96.02.084","url":null,"abstract":"Adiponectin is secreted by adipose tissue, associated with lipoprotein (LP) metabolism, down-regulated­ in insulin resistance states, and reduced in individuals suffering from obesity and cardiovascular diseases. Phospholipids and cholesterol are the main components of cell membranes and play a critical role in storage and secretory adipocyte functions. N-stearoylethanolamine (NSE) is a minor lipid affecting cell membrane lipids’ composition. Our study aimed to investigate plasma levels of adiponectin and cholesterol of low- and high-density LP (LDL and HDL) and adipocyte cholesterol-phospholipid (Chol-PL) composition of different age rats with high-fat diet (HFD)-induced obesity and insulin resistance and their changes under NSE administration. Our study demonstrated that chronic dietary fat overloading leads to obesity accompanied by impairment of glucose tolerance, a manifestation of dyslipidemia, and changes in plasma adiponectin levels in rats from two age groups (10-month-old and and 24-month-old). Prolonged HFD led to a reduction in plasma adiponectin levels and the growth of adipocyte cholesterol content in rats of different ages. A significant increase in plasma LDL-Chol level and main adipocyte PLs (phosphatidylcholine, phosphatidylethanolamine, sphingomyelin, and lysophosphatidylcholine) was observed in younger rats, whereas not detected in elder animals after dietary fats overloading. The decrease in the content of anionic phospholipids (phosphatidylinositol + phosphatidylserine) was also detected in 10-month-old HFD rats compared to the control animals. NSE administration positively affected the normalization of adiponectin levels in both age HFD groups. It significantly impacted the reduction of LDL-Chol levels and the growth of HDL-Chol concentration in the blood plasma of 10-month-old rats as well as PL-composition of young HFD rats and anionic PL restoring in 24-month-old rats. The positive effect on investigated parameters makes NSE a prospective agent for treating diet-induced and age-related metabolic disorders threatening cardiovascular diseases. Keywords: adiponectin, adipose tissue, aging, cholesterol, dyslipidemia, lipoproteins, N-stearoylethanolamine, phospholipids","PeriodicalId":23007,"journal":{"name":"The Ukrainian Biochemical Journal","volume":"9 15","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140658633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antioxidants as supplements during drug-induced thrombocytopenia: a comparative analysis of Vanillic acid, L-carnitine and Caripill™","authors":"M. Mithun, V. Rajashekaraiah","doi":"10.15407/ubj96.01.049","DOIUrl":"https://doi.org/10.15407/ubj96.01.049","url":null,"abstract":"Drug-induced thrombocytopenia (DIT) is a disorder where platelet count declines as an adverse effect of therapeutic drugs. Plant extract of C. papaya Caripill™ is known to elevate platelet count under thrombocytopenic conditions. To evaluate the contribution of supplements with antioxidant potential to treat DIT, the comparative study of Caripill™, vanillic acid L-carnitine effect on platelet count and indices of oxidative stress in a model of rat thrombocytopenia induced through oral administration of hydroxyurea was performed. Wistar rats were grouped into four categories with five animals in each group: control (without any treatment); control + antioxidants; thrombocytopenia; thrombocytopenia + antioxidants. The above-mentioned antioxidants were supplemented orally at 50 mg/kg for 7 days. The level of lipid peroxidation products­, superoxides, protein carbonyls and sulfhydryls, SOD and CAT activity in isolated platelets as oxidative stress markers, and indices of platelets aggregation and ATP secretion as functional markers were used. Vanillic acid was shown to be beneficial, similar to Caripill™, during hydroxyurea-induced thrombocytopenia by maintaining platelet functions, enhancing both the antioxidant capacity of platelets and its number. L-carnitine efficiently up-regulated the enzymatic antioxidants, maintained platelet functions and protected lipids and proteins from oxidation in thrombocytopenic rats, however, it could not improve the platelet count. These findings open new avenues for employing the studied antioxidants as supplements for therapeutic purposes.","PeriodicalId":23007,"journal":{"name":"The Ukrainian Biochemical Journal","volume":"22 17","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140436284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phosphatidic acid formation and signaling in plant cells","authors":"Y. Kolesnikov, S. Kretynin, V. S. Kravets, Y. K. Bukhonska","doi":"10.15407/ubj96.01.005","DOIUrl":"https://doi.org/10.15407/ubj96.01.005","url":null,"abstract":"This review conteins updated information on the structure, localization and regulation of phosphatidic acid (PA)-producing enzymes phospholipase D, phosphoinositide-specific and non-specific phospholipases C and diacylglycerol kinases is analyzed. The specific role of PA and PA-producing enzymes in plant stress signaling is discussed.","PeriodicalId":23007,"journal":{"name":"The Ukrainian Biochemical Journal","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140437038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In the footsteps of a biochemical luminary: the scientific legacy of Yakub Parnas","authors":"L. Kobylinska","doi":"10.15407/ubj96.01.103","DOIUrl":"https://doi.org/10.15407/ubj96.01.103","url":null,"abstract":"The article is dedicated to commemorating Yakub Karol Parnas, an eminent figure in biochemistry and a professor in the Department of Physiological (Biological) Chemistry at Lviv Medical University. It underscores the profound impact of Yakub Parnas on Ukrainian biochemistry, shedding light on intriguing facets­ of his personality during his highly productive two decades at Lviv University. His research significantly contributed to the unveiling of anaerobic glucose metabolism, later acknowledged as the Embden-Meyerhoff-Parnas pathway. Professor Yakub Parnas and his Lviv school conducted pivotal studies on enzymatic transformations associated with anaerobic muscle function and alcoholic fermentation, glycogen phosphorolysis, revelation of ATP synthesis within glycolysis, pioneering use of radioactively labeled phosphorus in biological experiments. Keywords: Biochemistry, Embden-Meyerhoff-Parnas pathway, Jakub Parnas, Lviv","PeriodicalId":23007,"journal":{"name":"The Ukrainian Biochemical Journal","volume":"46 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140437437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Мixed-ligand complexes of germanium – 3d-metal with 1-hydroxyethane-1,1-diphosphonic acid аnd 2,2′-bipyridine as modulators of Bacillus sp. IMV B-7883 elastase and fibrinogenase activity","authors":"O. Gudzenko, L. Varbanets, I. Seifullina, О. E. Martsynko, K. K. Tsymbalyuk","doi":"10.15407/ubj96.01.096","DOIUrl":"https://doi.org/10.15407/ubj96.01.096","url":null,"abstract":"We have previously shown that Bacillus sp. IMV B-7883 exhibits both elastase and fibrinogenolytic activity. One of the approaches to enhance enzymatic activity is the use of coordination compounds capable to affect enzyme’s activity or synthesis. The purpose of this work was to study the effect of mixed-ligand complexes of Ge(IV) – Co(II) (Ni(II), Cu(II)) with 1-hydroxyethane-1,1-diphosphonic acid аnd 2,2′-bipyridine on the activity of elastase and fibrinogenase purified from Bacillus sp. IMV B-7883. Previously synthesized and characterized mixed-ligand complexes and enzymes purified from the supernatant of the bacterial culture liquid were used in the study. Elastase activity was determined colorimetrically with the use of Congo red, fibrinogenase activity was estimated by fibrinogen hydrolysis measured by absorption at 275 nm. It was shown that complexes 1 (C132H164Co4Ge6N24O68P12) and 2 (C132H148Ge6N24Ni4O60P12) inhibited activity of Bacillus sp. IMV B-7883 elastase by 54 and 71% respectively, while complex 3 (C92H128Cu4Ge6N16O63P12) enhanced it by 30%. Stimulating effect of all three complexes on fibrinogenase activity was revealed. Thus, complex 1 and 2 activated the enzyme by more than 50% and complex 1 – by 19%. The data obtained indicate a complex mechanism of the studied complexes influence on enzymatic activity depending on both their composition and structure. Keywords: 1-hydroxyethane-1‚1-diphosphonic acid, 2‚2′-bipyridine, Bacillus sp. IMV B-7883, elastase, fibrinogenase, germanium – 3d-metal, mixed-ligand complexes","PeriodicalId":23007,"journal":{"name":"The Ukrainian Biochemical Journal","volume":"55 10","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140436774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multiple effects of angiostatins in injured cornea","authors":"V. Bilous, A. O. Tykhomyrov","doi":"10.15407/ubj96.01.037","DOIUrl":"https://doi.org/10.15407/ubj96.01.037","url":null,"abstract":"Prolonged inflammation and excessive neovascularization of the cornea due to severe injury can impair optical clarity and lead to vision impairment. Plasminogen kringle (K) fragments, known as angiostatins (AS), play a well-established role as inhibitors of neovascularization by suppressing pro-angiogenic signaling­. However, AS effects in the cornea, beyond inhibiting the angiogenesis, are still unexplored. In this study, we estimate the protective effect of two AS variants (K1-3 and K5) against alkali burn injury induced in rabbit and rat corneas. AS K1-3 in the single doses of 0.075 or 0.75 μg (0.1 or 1.0 μM, respectively) or 0.3 μg of AS K5 (1.0 μM) were applied locally as eye drops daily for 14 days after the injury. A significant regression of corneal vessels in-growth in injured eyes treated with AS was revealed. Western blot analysis of corneal tissue lysates revealed that injury-induced overexpression of protein markers­ of hypoxia (HIF-1α), angiogenesis (VEGF), tissue remodeling and fibrosis (MMP-9), autophagy (beclin-1) and endoplasmic reticulum stress (GRP-78) was significantly reduced under AS treatment. Besides, the level of tight junctions protein ZO-1 was shown to be up-regulated after the treatment of the damaged cornea with AS K1-3. Summarizing, our study uncovered novel biological functions of the kringle-containing plasminogen fragments indicating its beneficial effects during corneal healing in the experimental model of alkali burn. The data obtained can be helpful for the development of novel efficient formulations to manage complications of ocular surface injuries.","PeriodicalId":23007,"journal":{"name":"The Ukrainian Biochemical Journal","volume":"33 3","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140437216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Kinetic regularities of thiacalix[4]arene C-1087 inhibitory effect on the activity of Mg(2+)-dependent Ca(2+)-transporting ATP hydrolase in the plasma membrane of smooth muscle cells","authors":"Т. О. Veklich, О. V. Bevza, О. V. Maliuk, S. Kosterin, R. Rodik, S. Vyshnevskyi, V. І. Kalchenko","doi":"10.15407/ubj96.01.022","DOIUrl":"https://doi.org/10.15407/ubj96.01.022","url":null,"abstract":"The experiments with the suspension of plasma membranes of myometrium cells, treated with 0.1% digitonin solution, were used to study kinetic regularities of the inhibitory effect of tetra-N-phenylsulfonyl trifluoroacetamidine-thiacalixarene (С-1087) on the activity of Са2+,Mg2+-ATPase. The studies demonstrated the impact of C-1087 on the cumulative effect and the maximal velocity of ATP hydrolysis. No effect of С-1087 on the affinity between Са2+,Mg2+-ATPase, and АТР, affinity and cumulative effect of Ca ions and activation coefficient for Mg ions was revealed. A considerable decrease in the maximal velocity of ATP hydrolysis evidenced a complete non-competitive mechanism of inhibiting Са2+,Mg2+-АТРase activity with thiacalix[4]arene С-1087. Computer simulation demonstrated that thiacalix[4]arene С-1087 inhibiting effect on Са2+,Mg2+-ATPase may be conditioned by the cumulative effect of four spatially oriented N-sulfonylamidine groups on the upper rim of its macrocyclic platform.","PeriodicalId":23007,"journal":{"name":"The Ukrainian Biochemical Journal","volume":"23 8","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140437139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Free radical processes in the liver mitochondria of rats exposed to diethyl phthalate","authors":"O. Ketsa, A. P. Husliakova, M. Marchenko","doi":"10.15407/ubj96.01.073","DOIUrl":"https://doi.org/10.15407/ubj96.01.073","url":null,"abstract":"Diethyl phthalate (DEF) is a synthetic chemical widely used as plasticizer and additive in personal care and pharmaceutical products. Low-dose exposure to this xenobiotic over a long period contributes to its intake into the human body in a high doses. To date, studies of DEF influence on free radical processes in liver cells, in which it is not only metabolized but can also have a prooxidant effect, remain limited. The aim of our research was to determine ROS formation, the intensity of oxidative modification of proteins (OMP) and proteolytic activity in the mitochondrial fraction of liver tissue of rats exposed to diethyl phtalate. The experimental white outbred rats were divided into three groups: I – intact animals (control), II and III – rats administered DEF orally for three weeks at a doses that reflect the dose levels received by humans, namely 2.5 and 5.4 mg/kg b.w. respectively. The animals were euthanized on the 14th and 21st day after xenobiotic administration. The mitochondrial fraction from the rat liver was isolated and the level of superoxide and hydroxyl radicals, protein carbonyl derivatives, SH-groups and Schiff bases was determined. Proteolytic activity was assessed in the test of hemoglobin cleavage. It was shown that administration of DEF in a dose of 2.5 mg/kg initiated ROS generation and OMP intensification in the rat liver mitochondria only with prolonged administration for 21 days, whereas its administration in a dose of 5.4 mg/kg led to intensification of these processes already on day 14th followed by further amplification on day 21st. The activity of proteolytic enzymes in the mitochondrial fraction was found to be depended on the degree of DEF–induced OMP and was increased with minor OMP intensification but decreased with significant intensification of proteins free radical oxidation. Keywords: carbonyl derivatives, diethyl phthalate, liver, mitochondrial fraction, proteolytic enzymes, ROS, SH-groups","PeriodicalId":23007,"journal":{"name":"The Ukrainian Biochemical Journal","volume":"115 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-02-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140438190","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plasma amino acids pecularities and cardiometabolic risk factors in patients with coronary artery disease and atrial fibrillation","authors":"I. Melnychuk, M. Sharayeva, ,. O. V. Dolynna, O. V. Savchenko, V. Kramarova, V. Lyzogub","doi":"10.15407/ubj95.06.030","DOIUrl":"https://doi.org/10.15407/ubj95.06.030","url":null,"abstract":"Studies targeting small molecule metabolites (amino acids, sugars, nucleotides, lipids) in connections with gut microbiota metabolites that impact the host metabolome give a possibility to define a special metabolic signature of different diseases. Plasma amino acids (AA) profile is known to be a new promising biomarker for the screening of coronary artery disease (CAD) pathogenesis connected with gut dysbiosis. The aim of our study was to estimate the spectrum of plasma amino acids in CAD patients with atrial fibrillation (AF) and to check their connections with the gut microbiota metabolites. 300 patients were divided into three groups: CAD – 149 patients with CAD but without arrhythmias, CAD+AF – 123 patients with CAD and AF paroxysm and control group– 28 patients without CAD and arrhythmias. Plasma AA level was detected by ion exchange liquid column chromatography. Significant changes in the content of plasma Glutamate, Glutamine, Glycine, Alanine, Valine and Tyrosine and combinations Isoleucine+Leucine/Valine, Glycine+Serine, Glycine/Methionine, Phenylalanine/Tyrosine, Glutamine/Glutamate in CAD+AF patients were detected. A strong reliable connection between plasma AA spectrum and gut microbiota metabolites trimethylamine, trimethylamine-N-oxide and total amount of fecal short chain fatty acids was determined. The highly validated plasma AA combinations Isoleucine – Glycine (area under ROC-curve 0.8122) and Phenylalanine – Glycine (area under ROC-curve 0.8061) that can be used as the early markers of AF paroxysm in CAD patients were proposed. Keywords: atrial fibrillation, cardiometabolic risk factors, coronary artery disease, gut microbiota metabolites, plasma amino acids","PeriodicalId":23007,"journal":{"name":"The Ukrainian Biochemical Journal","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"138971046","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exometabolites of streptomycetes isolated from the Odesa bay exhibit a toxic effect against human cancer cell lines","authors":"V. Ivanytsia, T. V. Gudzenko, O. H. Gorshkova, G. V. Lisyutin, M. D. Shtenikov, N. Korotaeva, ,. K. S. Potapenko, I. V. Strashnova, T. V. Ivanytsia","doi":"10.15407/ubj95.06.097","DOIUrl":"https://doi.org/10.15407/ubj95.06.097","url":null,"abstract":"The aim of the work was to determine the cytotoxic activity of marine actinobacteria exometabolites on the human larynx adenocarcinoma Hep-2 and rhabdomyosarcoma RD cell lines. Exometabolites of 6 strains of Streptomyces sp. were extracted with ethyl acetate and their composition was determined with UltiMate™ 3000 BioRS UPLC System coupled to mass spectrometer. Cytotoxic activity of exometabolites against cancer cells was determined after 24 hours of incubation by microscopic examination of cell morphological changes and the degree of cell monolayer degeneration. Exometabolites from Streptomyces sp. Myt7b strain showed the highest cytotoxic activity and at concentration of 500.0 μg/ml caused up to 90.0% death of RD and Hep-2 cells. The presence of 53 identified compounds with potential cytotoxic activity, including enterocin, 6-prenyltryptophol, medelamine A and turbinaric acid was detected in Streptomyces sp. Myt7b exometabolome. It is concluded that this strain is a promising producer of compounds with anticancer activity. Keywords: cytotoxic activity, exometabolites, marine streptomycetes, RD and Hep-2 cancer cells","PeriodicalId":23007,"journal":{"name":"The Ukrainian Biochemical Journal","volume":"27 5","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139002807","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}