Exometabolites of streptomycetes isolated from the Odesa bay exhibit a toxic effect against human cancer cell lines

The Ukrainian Biochemical Journal Pub Date : 2023-12-14 DOI:10.15407/ubj95.06.097

V. Ivanytsia, T. V. Gudzenko, O. H. Gorshkova, G. V. Lisyutin, M. D. Shtenikov, N. Korotaeva, ,. K. S. Potapenko, I. V. Strashnova, T. V. Ivanytsia

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Abstract

The aim of the work was to determine the cytotoxic activity of marine actinobacteria exometabolites on the human larynx adenocarcinoma Hep-2 and rhabdomyosarcoma RD cell lines. Exometabolites of 6 strains of Streptomyces sp. were extracted with ethyl acetate and their composition was determined with UltiMate™ 3000 BioRS UPLC System coupled to mass spectrometer. Cytotoxic activity of exometabolites against cancer cells was determined after 24 hours of incubation by microscopic examination of cell morphological changes and the degree of cell monolayer degeneration. Exometabolites from Streptomyces sp. Myt7b strain showed the highest cytotoxic activity and at concentration of 500.0 μg/ml caused up to 90.0% death of RD and Hep-2 cells. The presence of 53 identified compounds with potential cytotoxic activity, including enterocin, 6-prenyltryptophol, medelamine A and turbinaric acid was detected in Streptomyces sp. Myt7b exometabolome. It is concluded that this strain is a promising producer of compounds with anticancer activity. Keywords: cytotoxic activity, exometabolites, marine streptomycetes, RD and Hep-2 cancer cells

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从奥德萨海湾分离出的链霉菌外代谢产物对人类癌细胞系具有毒性作用

这项研究旨在确定海洋放线菌外代谢产物对人类喉腺癌 Hep-2 和横纹肌肉瘤 RD 细胞系的细胞毒活性。用乙酸乙酯提取 6 株链霉菌的外代谢产物，并用 UltiMate™ 3000 BioRS UPLC 系统和质谱仪测定其成分。培养 24 小时后，通过显微镜检查细胞形态变化和细胞单层变性程度，确定外代谢产物对癌细胞的细胞毒活性。来自链霉菌 Myt7b 菌株的外代谢产物显示出最高的细胞毒性活性，在浓度为 500.0 μg/ml 时，可导致 RD 和 Hep-2 细胞 90.0% 的死亡。在链霉菌 Myt7b 的外代谢组中发现了 53 种具有潜在细胞毒性活性的化合物，包括肠毒素、6-prenyltryptophol、medelamine A 和 turbinaric acid。结论是该菌株是一种具有抗癌活性的化合物生产者。关键词：细胞毒性活性、外代谢产物、海洋链霉菌、RD 和 Hep-2 癌细胞

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The Ukrainian Biochemical Journal

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