Small Methods最新文献_第3页

Label-Free and Microplate-Based Dissection of Glycan-Virus Interactions Using Polymer-Tethered Glyconanoparticles. 无标记和基于微孔板的糖聚糖-病毒相互作用的聚合物连接糖聚糖颗粒解剖。

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-07-02 DOI: 10.1002/smtd.202500214

Sarah-Jane Richards, Simona Chessa, Lloyd Sayer, Irina Ivanova, Sanaz Ahmadipour, Alexander N Baker, Marc Walker, Simone Dedola, Katherine A Scott, Oliver Dibben, Robert A Field, Matthew I Gibson

{"title":"Label-Free and Microplate-Based Dissection of Glycan-Virus Interactions Using Polymer-Tethered Glyconanoparticles.","authors":"Sarah-Jane Richards, Simona Chessa, Lloyd Sayer, Irina Ivanova, Sanaz Ahmadipour, Alexander N Baker, Marc Walker, Simone Dedola, Katherine A Scott, Oliver Dibben, Robert A Field, Matthew I Gibson","doi":"10.1002/smtd.202500214","DOIUrl":"https://doi.org/10.1002/smtd.202500214","url":null,"abstract":"Influenza viruses use haemagglutinins (HA) to target host sialic acids in the respiratory tract as do other pathogens, including coronaviruses, which engage using spike protein. The host adaptation of the HA protein, which leads to the accumulation of mutations, is a key descriptor of individual influenza strains, which aids zoonosis and is crucial in vaccine development. How each strain targets glycans is crucial to understanding function, designing new therapies, and optimizing candidates for vaccine development. Here, it is demonstrated that polymer-tethered plasmonic (gold) glyconanoparticles can be deployed for rapid evaluation of whole influenza virus binding, readable by simple UV-vis within a microwell plate as a low-tech alternative to printed microarrays. It is also demonstrated that the synthetic methodology is compatible with large branched glycans from chemoenzymatic synthesis, allowing a wider range of glycan structures to be probed. Particles are obtained by a modular capture and immobilisation process and used to interrogate the binding of five influenza strains as proof of concept. These results show that glycosylated nanoparticle probes are suitable for the rapid interrogation of live virus to map how glycan structure impacts binding and can enable at-bench, rapid virus/glycan binding readouts and aid the development of interventions for influenza and other viruses.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2500214"},"PeriodicalIF":10.7,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144551544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

3D-Printed Copper-Based Gas Diffusion Electrodes with a Tunable Bilayer Architecture for Controlled CO₂ Electroreduction Selectivity. 3d打印铜基气体扩散电极可调双层结构控制CO2电还原选择性。

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-07-02 DOI: 10.1002/smtd.202500648

Leying Huang, Meng Zhang, Xiuping Zhu

{"title":"3D-Printed Copper-Based Gas Diffusion Electrodes with a Tunable Bilayer Architecture for Controlled CO2 Electroreduction Selectivity.","authors":"Leying Huang, Meng Zhang, Xiuping Zhu","doi":"10.1002/smtd.202500648","DOIUrl":"https://doi.org/10.1002/smtd.202500648","url":null,"abstract":"Electrochemical CO2 reduction reaction (CO2RR) offers a promising pathway for converting CO2 into value-added chemicals, contributing to climate change mitigation and the advancement of sustainable chemical production. In CO2RR flow cell systems, developing gas diffusion electrodes (GDEs) with enhanced electrochemical performance is crucial for enabling commercial applications. However, the exploration of copper-based self-supported GDEs is constrained by a narrow selection of substrates. 3D printing technology offers an approach to creating intricate structural components, but its integration with GDE fabrication remains unexplored. Here, a 3D-printed copper-based GDE, fabricated via fused deposition modeling (FDM) and featuring a tunable porous structure, is successfully applied in a CO2RR flow cell. Under specified operating conditions, adjusting the thickness of the electrode's solid layer influenced H2 selectivity, while modifying the thickness or density of the grid-like infill layer allowed for tuning C2H4 and CO selectivity. Notably, the S2I2 electrode configuration, with 0.2 mm-thick solid and infill layers, achieves 47% C2+ product selectivity at a current density of 200 mA cm-2. This work demonstrates the potential of 3D printing for designing architecture-tailored GDEs and tuning CO2 electroreduction product distributions.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2500648"},"PeriodicalIF":10.7,"publicationDate":"2025-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144537601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Interface Engineering Modulation of 3D Layered Ti₃C₂T_x/MoS_1.5Se_0.5 Composite Aerogel for Advanced Anode in Sodium-Ion Batteries. 钠离子电池高级负极用三维层状Ti3C2Tx/MoS1.5Se0.5复合气凝胶的界面工程调制

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-07-02 DOI: 10.1002/smtd.202500985

Xinhui Jin, Dejie Mo, Xikun Zhang, Lirong Feng, Baolian Su, Xiaohui Guo

引用次数: 0

Modulating Phase Separation via Multiple Hydrogen Bonding in Polyurethane-Based Gel Polymer Electrolytes for All-Solid-State Supercapacitors. 在全固态超级电容器用聚氨酯基凝胶聚合物电解质中通过多重氢键调制相分离。

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-07-01 DOI: 10.1002/smtd.202500881

Puji Lestari Handayani, U Hyeok Choi

{"title":"Modulating Phase Separation via Multiple Hydrogen Bonding in Polyurethane-Based Gel Polymer Electrolytes for All-Solid-State Supercapacitors.","authors":"Puji Lestari Handayani, U Hyeok Choi","doi":"10.1002/smtd.202500881","DOIUrl":"https://doi.org/10.1002/smtd.202500881","url":null,"abstract":"Hybrid gel polymer electrolytes (GPEs) based on thermoplastic polyurethane (PU) and ionic liquid (IL) are successfully synthesized by incorporating silica nanoparticles through a one-pot in situ sol-gel process. The ionic liquid, 1-butyl-3-methylimidazolium bis(trifluoromethylsulfonyl)imide [(BMIM)+(TFSI)-], acts as a miscible solvent for both the hard and soft segments of PU, simultaneously serving as an ionic charge carrier and plasticizer. Fourier transform infrared (FTIR) Spectroscopy and differential scanning calorimetry (DSC) analyses reveal that increasing the IL concentration weakens the hydrogen bonding interactions between hard-hard and hard-soft segments, leading to a reduction in glass transition temperature and suppression of phase separation. Furthermore, incorporating silica nanoparticles as phase-modulators by creating hydrogen bonding between -Si-OH with TFSI- anion, inducing phase-separation, thus enhancing the room temperature ionic conductivity of the GPE, and at the same time, the mechanical strength of the GPE also improves upon the addition of 0.02 wt.% SN. The all-solid-state supercapacitors using hybrid GPE delivered a high energy density (ED = 183 Wh kg-1) and power density (PD = 7 kW kg-1) within an extended voltage window of up to 3.5 V, along with excellent cycling stability, retaining ≈98% of their initial capacitance after 12, 000 cycles. These results indicate that the GPEs can be promising candidates for future all-solid-state energy storage devices.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2500881"},"PeriodicalIF":10.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144525755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reinforced Capacity and Cycling Stability of CoTe Nanoparticles Anchored on Ti₃C₂ MXene for Anode Material. ti3c2mxene固载CoTe纳米颗粒负极材料的增强性能和循环稳定性。

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-07-01 DOI: 10.1002/smtd.202500725

Ramesh Subramani, Su-Yang Hsu, Wei-Hsiang Huang, Zhiwei Hu, Kueih-Tzu Lu, Jin-Ming Chen

{"title":"Reinforced Capacity and Cycling Stability of CoTe Nanoparticles Anchored on Ti3C2 MXene for Anode Material.","authors":"Ramesh Subramani, Su-Yang Hsu, Wei-Hsiang Huang, Zhiwei Hu, Kueih-Tzu Lu, Jin-Ming Chen","doi":"10.1002/smtd.202500725","DOIUrl":"https://doi.org/10.1002/smtd.202500725","url":null,"abstract":"Developing high-performance anode materials is critical for lithium-ion batteries (LIBs) to meet consumers' demands. Cobalt tellurides (CoTe) exhibit promising electrochemical properties due to their higher theoretical capacity compared to commonly used graphite anodes. However, their practical application is hindered by poor electrical conductivity, agglomeration of nanoparticles, and significant volume changes during charge-discharge cycling. To overcome these challenges, CoTe nanoparticles are synthesized and anchored on Ti3C2 MXene (CoTe@Ti3C2) via a facile hydrothermal approach. The integration of CoTe nanoparticles with Ti3C2 nanosheets leverages their synergistic advantages: Ti3C2 MXene serves as a conductive substrate, improving electrical conductivity, reducing CoTe agglomeration, and accommodating volume changes, while CoTe nanoparticles prevent Ti3C2 nanosheet restacking. As a result, compared to the CoTe electrode, the CoTe@Ti3C2 anode exhibits an exceptional capacity increase, exceeding tenfold and reaching 698 mAh g-1 after 1000 cycles at 0.1 A g-1. Additionally, the CoTe@Ti3C2 anode demonstrates long-term cycling stability over 1300 cycles at 1 A g-1. In situ synchrotron X-ray diffraction and in situ X-ray absorption spectroscopy elucidate the insights into the charge storage mechanisms. The superior electrochemical performance of CoTe@Ti3C2 highlights its potential as a high-performance anode material for next-generation LIBs.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2500725"},"PeriodicalIF":10.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144525758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Near-Infrared-Triggered Nanodroplets for Imaging-Guided Macrophage-Mediated Cancer Immunotherapy. 近红外触发纳米液滴成像引导巨噬细胞介导的癌症免疫治疗。

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-07-01 DOI: 10.1002/smtd.202401679

Junxi Yi, Meenakshi Chauhan, Yang Zhao, Yun-Sheng Chen

{"title":"Near-Infrared-Triggered Nanodroplets for Imaging-Guided Macrophage-Mediated Cancer Immunotherapy.","authors":"Junxi Yi, Meenakshi Chauhan, Yang Zhao, Yun-Sheng Chen","doi":"10.1002/smtd.202401679","DOIUrl":"https://doi.org/10.1002/smtd.202401679","url":null,"abstract":"Immunotherapy has transformed cancer treatment; however, solid tumors often evade checkpoint blockade by co-opting tumor-associated macrophages (TAMs) and creating a hypoxic, immunosuppressive tumor microenvironment. Here, we report a laser-triggered theranostic nanodroplet capable of simultaneously remodeling the tumor microenvironment and visualizing therapeutic responses in real-time. These double-emulsion perfluorocarbon droplets encapsulate clinically translatable components: a toll-like receptor-7/8 agonist and near-infrared indocyanine green dye. Upon activation by a near-infrared laser pulse, the nanodroplets transform into echogenic microbubbles, facilitating imaging and targeted therapeutic release. In a mouse model of human triple-negative breast cancer, a single nanodroplet injection followed by periodic illumination repolarized M2-like TAMs into an M1 phenotype, alleviated intratumoral hypoxia, and synergistically anti-programmed-cell-death-protein-1 therapy, resulting in a six-fold reduction in tumor size compared to checkpoint blockade alone. Concurrent non-invasive dual-modal ultrasound and multispectral photoacoustic imaging tracked nanodroplet biodistribution, macrophage recruitment, and oxygen saturation longitudinally, enabling real-time guidance of dosing schedules without biopsy. The nanodroplets demonstrated greater than 60% loading efficiency, minimal hemolysis, and high biocompatibility in vitro. By coupling spatiotemporally controlled immunomodulation with quantitative imaging, this platform addresses critical challenges in treating refractory solid tumors and provides a roadmap for adaptive, image-guided combination immunotherapy.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2401679"},"PeriodicalIF":10.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144525756","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Transformation of 1D/2D High-Surface-Area Hierarchical Titanium Sulfate Structures to Stable, Morphology-Preserving Titania with Tailored Properties. 一维/二维高表面积分层硫酸钛结构转化为具有定制性能的稳定、保持形态的二氧化钛。

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-07-01 DOI: 10.1002/smtd.202500168

Katelyn Sowards, J Reveles, Hector Medina

{"title":"Transformation of 1D/2D High-Surface-Area Hierarchical Titanium Sulfate Structures to Stable, Morphology-Preserving Titania with Tailored Properties.","authors":"Katelyn Sowards, J Reveles, Hector Medina","doi":"10.1002/smtd.202500168","DOIUrl":"https://doi.org/10.1002/smtd.202500168","url":null,"abstract":"This report outlines a novel, facile process for the transformation of hierarchical enhanced surface area structures (HESAS) of titanium sulfate into titania. The transformation process preserves the HESAS morphology while providing tunable enhanced properties, based on the phase and degree of transformation. To demonstrate our process, a controlled thermo-chemical transformation strategy is implemented using four maximum temperatures (650, 750, 850, and 950 °C) in natural air or argon-rich environments, under various heating rates, and for two types of precursor HESAS. The resulting titania HESAS are characterized using scanning electron microscopy (SEM), energy dispersive spectroscopy (EDS), and thin-film X-ray diffraction (XRD). Furthermore, both ab initio and semi-empirical quantum mechanics computational studies are conducted to provide insights into the diffusion mechanisms involved and the associated energetics. The transformed materials exhibit retention of the hierarchical features from the precursor HESAS. Furthermore, the degree of anatase or rutile formed is controlled based on the thermal kinetics of the process. Computational studies show that SO3 release is the main mechanism underlying the transformation, with the removal energy barrier increasing with the number of SO3 released. This work reveals a pathway for a scalable, low-cost manufacturing process for the design and fabrication of advanced titania-based photocatalytic materials with tailored properties.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2500168"},"PeriodicalIF":10.7,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144525759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Performance Regulation of Sulfonated Poly(ether ether ketone) for Preparing Multi-Adaptive Electrolyte and Battery Separator of Wide-Temperature Flexible Zn-Air Batteries. 磺化聚醚醚酮制备宽温柔性锌空气电池多自适应电解液和电池隔膜的性能调控

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-06-30 DOI: 10.1002/smtd.202500884

Zhenhua Ren, Jiaqi Niu, Xiujuan Zhang, Xiaoqiang Liu, Jadranka Travas-Sejdic

{"title":"Performance Regulation of Sulfonated Poly(ether ether ketone) for Preparing Multi-Adaptive Electrolyte and Battery Separator of Wide-Temperature Flexible Zn-Air Batteries.","authors":"Zhenhua Ren, Jiaqi Niu, Xiujuan Zhang, Xiaoqiang Liu, Jadranka Travas-Sejdic","doi":"10.1002/smtd.202500884","DOIUrl":"https://doi.org/10.1002/smtd.202500884","url":null,"abstract":"Herein, sulfonated poly(ether ether ketone) (SPEEK) is employed to fabricate both the separator and gel electrolyte for solid zinc-air batteries (ZABs), leveraging its rigid backbone and abundant sulfonic acid groups in side chains of SPEEK. Incorporating glycerol into the separator (making SPEEKG-Zn2+) enhances its low-temperature performance. In the SPEEKG-Zn2+ separator, the sulfonic acid groups of SPEEKG-Zn2+ facilitate uniform deposition of Zn2+ on Zn anode and suppress OH- transfer through interacting with Zn2+. This dual functionality effectively minimizes dendrite growth and chemical corrosion, as confirmed by experiments and theoretical calculation. Additionally, the gel electrolyte is prepared with SPEEK membrane and crosslinked chitosan-glycerol gel. The electrolyte exhibits excellent mechanical properties, thermal stability and high ionic conductivity (51.04 ± 0.3 mS cm-1). ZABs with the electrolyte and SPEEKG-Zn2+ separator shows stable cyclic performance up to 56 h at 40 °C, high power density of 171.6 mW cm-1, specific capacity of 896.1 mAh g-1 at 60 °C, and wide temperature adaptability in -40 to 60 °C, which are superior to those of the recently reported ZABs. These properties are highly desirable for flexible ZABs functioning effectively under extreme temperature conditions.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2500884"},"PeriodicalIF":10.7,"publicationDate":"2025-06-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144525757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-Performance Flexible 2D Tellurium Semiconductor Grown by Isolated Plasma Soft Deposition for Wearable and Flexible Temperature Sensors. 可穿戴柔性温度传感器用隔离等离子体软沉积制备高性能柔性二维碲半导体。

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-06-29 DOI: 10.1002/smtd.202500379

Tae-Yang Choi, Jun-Hyeok Kang, Jong-Hyun Jang, Han-Ki Kim

{"title":"High-Performance Flexible 2D Tellurium Semiconductor Grown by Isolated Plasma Soft Deposition for Wearable and Flexible Temperature Sensors.","authors":"Tae-Yang Choi, Jun-Hyeok Kang, Jong-Hyun Jang, Han-Ki Kim","doi":"10.1002/smtd.202500379","DOIUrl":"https://doi.org/10.1002/smtd.202500379","url":null,"abstract":"High-quality flexible 2D tellurium (Te) semiconductors on a six-inch Si wafer and polyethylene terephthalate substrate using the isolated plasma soft deposition (IPSD) technique are successfully fabricated. Unlike conventional sputtering systems, the IPSD process minimizes direct plasma irradiation and plasma damage, thereby preserving the unique helical chain structure of the 2D Te layer. The integration of oxygen plasma treatment and in situ substrate heating significantly enhanced both the adhesion and crystallinity of the 2D Te layer. The optimized 2D Te layer exhibited exceptional properties, including a high carrier mobility of 103 cm2 V-1 s-1, a smooth surface roughness of 0.778 nm, and a critical bending radius of 12 mm. When integrated into temperature sensors, the 2D Te/PET demonstrated high sensitivity, exhibiting a negative temperature coefficient response across the 20-40 °C range. Moreover, the IPSD-grown 2D Te layer demonstrated outstanding mechanical flexibility, with minimal resistance changes (<4%) during both bending and rolling tests. Long-term stability assessments conducted over 100 days revealed resistance variations of less than 1%, highlighting the material's robust reliability. These findings position the IPSD process as a promising physical vapor deposition technique for scalable fabrication of large-area 2D Te layers, enabling their integration into wearable and flexible electronic devices.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2500379"},"PeriodicalIF":10.7,"publicationDate":"2025-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144525753","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comprehensive Osteosarcoma Treatment with Multifunctional Composite Hydrogels Enabling Combined Photothermal Cancer Ablation and Osteoinductive Tissue Regeneration. 多功能复合水凝胶联合光热癌消融和骨诱导组织再生治疗骨肉瘤。

IF 10.7 2区材料科学

Small Methods Pub Date : 2025-06-29 DOI: 10.1002/smtd.202500617

Hayeon Byun, Taeyeon Hwang, Hyoryong Lee, Yun-Jung Choi, Dong-Jae Kim, Eunji Park, Eunhyung Kim, Sukho Park, Heungsoo Shin

{"title":"Comprehensive Osteosarcoma Treatment with Multifunctional Composite Hydrogels Enabling Combined Photothermal Cancer Ablation and Osteoinductive Tissue Regeneration.","authors":"Hayeon Byun, Taeyeon Hwang, Hyoryong Lee, Yun-Jung Choi, Dong-Jae Kim, Eunji Park, Eunhyung Kim, Sukho Park, Heungsoo Shin","doi":"10.1002/smtd.202500617","DOIUrl":"https://doi.org/10.1002/smtd.202500617","url":null,"abstract":"Osteosarcoma treatment can lead to considerable loss of bone tissue, creating a challenging microenvironment for recovery. Here, a novel biomaterial is described for tumor treatment via photothermal therapy and bone-tissue regeneration. Multifunctional composite hydrogels can be fabricated by incorporating mineralized magnetic fibers (G-mMFs) into a gelatin-genipin hydrogel. The G-mMFs exhibit notable temperature increases in response to near-infrared irradiation, and superior disruption of tumor tissue follows hyperthermia therapy in a tumor-bearing mouse model. G-mMFs protect stem cells from the oxidative stress anticipated after tumor ablation, following significant increases in catalase and anti-apoptotic gene expression. G-mMFs demonstrate enhanced osteoinductivity, with nearly 90% of human adipose-derived stem cells exhibiting osteogenic markers. Adenosine signaling-mediated osteogenesis and restoration of osteogenesis under oxidative stress can be demonstrated through stem-cell differentiation in the presence of H2O2. In vivo, regeneration of bone tissue can be assessed using a calvarial bone-defect mouse model, with nearly twice the amount of bone formation in the G-mMF group compared with mice without implantation, along with a more mature bone-tissue structure. Collectively, these study results present G-mMFs as a multifunctional biomaterial that simultaneously addresses tumor ablation and bone regeneration, offering a promising strategy for the comprehensive treatment of osteosarcoma.","PeriodicalId":229,"journal":{"name":"Small Methods","volume":" ","pages":"e2500617"},"PeriodicalIF":10.7,"publicationDate":"2025-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144525751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0