The Journal of Microbiology, Biotechnology and Food Sciences最新文献_第4页

CONJUGATED LINOLEIC ACID-ENRICHED DAIRY PRODUCTS: A REVIEW 富含共轭亚油酸的乳制品:综述

The Journal of Microbiology, Biotechnology and Food Sciences Pub Date : 2021-04-01 DOI: 10.15414/JMBFS.3609

M. Ivanova

{"title":"CONJUGATED LINOLEIC ACID-ENRICHED DAIRY PRODUCTS: A REVIEW","authors":"M. Ivanova","doi":"10.15414/JMBFS.3609","DOIUrl":"https://doi.org/10.15414/JMBFS.3609","url":null,"abstract":"Conjugated linoleic acid (CLA) is a family of more than 28 isomers of linoleic acid wherein the isomers cis-9, trans-11 (rumenic acid) and trans-10, cis-12 are the most abundant. It is associated with a number of potential health benefits for human organism. Many foods are a good source of it but it is mosty found in meat and dairy products, derived from ruminants. Dairy products contain CLA in different amounts. The enrichment of these products with CLA is appropriate given the lower CLA content in these products in comparison with the recommended health intake. Modification of CLA concentration can be done by specific animal feeding and diet modification, by direct CLA supplementation in the form of oils or by addition of specific starter culture. The influence of technological treatments on the stability of the final product during storage and maturation is still not completely elucidated. There is a need for further studies on the physiological effects of CLA isomers on humans. The purpose of this review is to summarize the possibilities for increasing CLA content in milk and dairy products and to determine the possible effects of this enrichment on product stability – sensory, chemical, microbiological profile, shelf life and potential health effects of the obtained products.","PeriodicalId":22746,"journal":{"name":"The Journal of Microbiology, Biotechnology and Food Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79134387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

BIOLUMINOMETRIC ASSAY OF CELL ATP DETECTION UNDER STRESS CONDITIONS AND IN THE ASSESSMENT OF CLEANNESS OF THE FOOD PRODUCING COMPANY 胁迫条件下细胞atp检测及食品生产企业清洁度评价的生物荧光分析

The Journal of Microbiology, Biotechnology and Food Sciences Pub Date : 2021-04-01 DOI: 10.15414/JMBFS.2584

M. Požgajová, H. Ďúranová

{"title":"BIOLUMINOMETRIC ASSAY OF CELL ATP DETECTION UNDER STRESS CONDITIONS AND IN THE ASSESSMENT OF CLEANNESS OF THE FOOD PRODUCING COMPANY","authors":"M. Požgajová, H. Ďúranová","doi":"10.15414/JMBFS.2584","DOIUrl":"https://doi.org/10.15414/JMBFS.2584","url":null,"abstract":"Detection and tracking of microorganism’s behavior in natural environments and under stressed conditions have been nowadays accelerated by the requirement for the risk assessment associated with environmental contamination. Luminescence-based techniques, for they cost efficiency, fastness, reliability and reproducibility are particularly appropriate for such studies. We used bioluminescence sensing assay to determine ATP levels in the yeast Schizosaccharomyce pombe. Determination of adenosine concentration per cell was assessed in the yeast cells counted prior to the experiment. To test the methodology, model organism was exposed to life-threatening concentration of heavy metals cadmium and nickel for three hours. Afterwards, changes in the ATP levels were detected and compared to the control. Strikingly, our results revealed that such conditions lead to considerable increase in the production of ATP by mitochondria compared to untreated cells. Normal level of ATP in the control increased from the basal 9.92x10-16 M to 2.90x10-15 M after Cd exposure and to 7.58x10-15 M upon Ni treatment. The methodology was subsequently used for assessment of cleanness of margarine producing company devices after regular sanitation procedure. Detection of only negligible amount of the ATP signal confirmed good cleaning strategy in the tested food processing company.","PeriodicalId":22746,"journal":{"name":"The Journal of Microbiology, Biotechnology and Food Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78603161","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Substantial improvement of protease production using statistical optimization and Downstream approach from the strain Bacillus toyonensis VKB5 sp., isolated from the host Actinidia delicosa 利用统计优化和下游方法对从宿主猕猴桃中分离的toyonensis VKB5 sp.菌株蛋白酶产量的实质性提高

The Journal of Microbiology, Biotechnology and Food Sciences Pub Date : 2021-04-01 DOI: 10.15414/JMBFS.3721

Venkataraman Kumar

引用次数: 2

VALIDATION OF THE ENZYME-LINKED IMMUNOSORBENT ASSAY FOR EMIQUANTITATIVE DETERMINATION OF IgM ANTIBODIES AGAINST CHLAMYDIA TRACHOMATIS 用于沙眼衣原体IgM抗体准定量测定的酶联免疫吸附法的验证

The Journal of Microbiology, Biotechnology and Food Sciences Pub Date : 2021-04-01 DOI: 10.15414/JMBFS.2313

A. Besarab, V. Motronenko, E. Bespalova, I. Nastenko

{"title":"VALIDATION OF THE ENZYME-LINKED IMMUNOSORBENT ASSAY FOR EMIQUANTITATIVE DETERMINATION OF IgM ANTIBODIES AGAINST CHLAMYDIA TRACHOMATIS","authors":"A. Besarab, V. Motronenko, E. Bespalova, I. Nastenko","doi":"10.15414/JMBFS.2313","DOIUrl":"https://doi.org/10.15414/JMBFS.2313","url":null,"abstract":"The object of the following study is representation of the scientific and methodical foundation of the procedure of validation of means of serum in vitro diagnosis basing on the sample of ELISA for semiquantification of specific Chlamydia trachomatis IgM-antibodies. Validation characteristics (precision, diagnostic and analytic specificity, diagnostic sensitivity, relative linearity) were defined both at the beginning of the release of diagnostic set and the expiration date (as the element of stability study). The values of diagnostic sensitivity and specificity, defined via the in-process set of serums (20 positive and 50 negative) comprised 100%. The presence of other classes of Chlamydia antibodies in samples didn’t influence the results of ELISA for specific IgM antibodies. Linearity of the method was sufficient. The intra-assay variation of results is between 3.1% to 6.4%, and intra-assay precision was from 1.5% to 8.4%, staying acceptable (≤ 10%) both at the moment of release and during expiration date. The ELISA method is validated, and the diagnostic kit is regarded as stable during 1 year.","PeriodicalId":22746,"journal":{"name":"The Journal of Microbiology, Biotechnology and Food Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72790390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

STUDYING THE EFFECT OF CHITOSAN ON BACILLUS CEREUS PRODUCING CEREULIDE TOXIN IN MILK AND SOME DAIRY DESSERTS 研究壳聚糖对乳及某些乳制甜点中蜡样芽孢杆菌产生蜡样毒素的影响

The Journal of Microbiology, Biotechnology and Food Sciences Pub Date : 2021-04-01 DOI: 10.15414/JMBFS.3417

N. A. Elnisr, W. Elsherif, A. H. E. Hendy, N. M. Wahba

{"title":"STUDYING THE EFFECT OF CHITOSAN ON BACILLUS CEREUS PRODUCING CEREULIDE TOXIN IN MILK AND SOME DAIRY DESSERTS","authors":"N. A. Elnisr, W. Elsherif, A. H. E. Hendy, N. M. Wahba","doi":"10.15414/JMBFS.3417","DOIUrl":"https://doi.org/10.15414/JMBFS.3417","url":null,"abstract":"A total of 150 samples of raw milk and two types of refrigerated processed dairy desserts dishes (cooked rice with milk and Mehalabia dishes) (50 for each) were collected from dairy shops and local restaurants in Assuit City, Egypt. ISO7932 method was used for enumeration of B. cereus, afterwards a PCR was performed to confirm the presence of cereulide toxin (ces) gene. In addition, the effect of different concentrations of chitosan and chitosan nanoparticles (CNPs) (0.05, 1%) on B. cereus was studied in pasteurized milk.The shelf life was evaluated by clot on boiling test and Ph value. Their pathological effect was detected by orally administration to experimental rats to investigate their adverse effect on rats liver and intestine using histopathological examination. The most prominent potent bactericidal effect was observed inCNPs 1%, B. cereus count reached to undetectable level at 5th day of refrigerated storage. The pasteurized milk inoculated with B. cereus, chitosan and CNPs showed some pathological lesions in rats treated with B. cereus,while the chitosan 0.5% had antibacterial activities without cytotoxic effect on rat tissues, alternatively to CNPs 1%, which exist edematous tips of intestinal villi and mild kupffer cells activation in liver.Therefore, our results contribute data that are primary to indicate the risk of food poisoning due to B. cereus and trials to control that in food by careful using of nanotechnology. However, the additional researches are needed to safe using of this technology even on natural nano-materials as it at nano-size gain new qualities.","PeriodicalId":22746,"journal":{"name":"The Journal of Microbiology, Biotechnology and Food Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87570019","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

BIO-PROFILING OF A POTENTIAL ANTIMYCOBACTERIAL BACTERIOCIN PRODUCED BY BACILLUS SUBTILIS (MK733983) OF ETHNOMEDICINAL ORIGIN 民族医药来源的枯草芽孢杆菌(mk733983)产生的潜在抗细菌细菌素的生物特性分析

The Journal of Microbiology, Biotechnology and Food Sciences Pub Date : 2021-04-01 DOI: 10.15414/JMBFS.3259

Shanti

引用次数: 2

EFFECT OF DIETARY SUPPLEMENTATION WITH SEAWEED AND POLYPHENOLS MIXTURE ON ANTIOXIDANT STATUS, CONCENTRATION AND MOTILITY OF RABBIT SPERMATOZOA 饲粮中添加海藻和多酚混合物对兔精子抗氧化状态、浓度和活力的影响

The Journal of Microbiology, Biotechnology and Food Sciences Pub Date : 2021-04-01 DOI: 10.15414/JMBFS.2179

N. Knížatová, M. Massányi, K. Tokárová, F. Vizzarri, P. Massányi, Ľ. Ondruška, N. Lukáč

{"title":"EFFECT OF DIETARY SUPPLEMENTATION WITH SEAWEED AND POLYPHENOLS MIXTURE ON ANTIOXIDANT STATUS, CONCENTRATION AND MOTILITY OF RABBIT SPERMATOZOA","authors":"N. Knížatová, M. Massányi, K. Tokárová, F. Vizzarri, P. Massányi, Ľ. Ondruška, N. Lukáč","doi":"10.15414/JMBFS.2179","DOIUrl":"https://doi.org/10.15414/JMBFS.2179","url":null,"abstract":"In recent years, many studies have been focused on natural substances that could have effect on health of animals. We investigated effect of extract consisting mainly of polyphenols, brown algae and plant polysaccharides on the reproduction potential of male rabbits (New Zealand white breed) during 60 days long dietary experiment. The rabbits were divided into three groups. Control was fed a basal diet, whereas the second and third group were supplemented with seaweed and polyphenols mixture: T1 = 0.3% and T2 = 0.6% respectively. We observed that sperm concentration in both experimental groups increased in comparison to the control group. Results of the CASA analysis showed enhanced motility (C = 85.09±7.53%, T1 = 87.21±8.25%, T2 = 89.38±8.02%) and progressive motility (C = 74.28±12.6%, T1 = 79.07±13.89%, T2 = 81.28±11.37%) in experimental groups supplemented with combination of algae and polyphenols in comparison with the control group. While monitoring ferric reducing ability of plasma (FRAP), we found the highest value in T1 group, changes were insignificat. An increase in GPx activity was measured in experimental groups in comparison with the control group with major difference in T1 group. In experimental groups, we determined an increase in activity of superoxide dismutase (SOD) in comparison with control group, the difference was significant in T2 group (C = 0.26±0.11 U/mg TP; T2 = 0.31±0.09 U/mg TP). In conclusion, our studies suggest that dietary supplementation with brown seaweed and plant polyphenols mixture may be potentially useful for enhancement of sperm motility and protection against oxidative stress.","PeriodicalId":22746,"journal":{"name":"The Journal of Microbiology, Biotechnology and Food Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76809774","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

BIOCONVERSION OF WINE POMACE BY LENTINUS EDODES IN A SOLID-STATE SYSTEM BIOCONVERTION OF WINE POMACE 固态系统中香菇对酒渣的生物转化

The Journal of Microbiology, Biotechnology and Food Sciences Pub Date : 2021-04-01 DOI: 10.15414/JMBFS.3006

H. Yıldırım

引用次数: 0

DRYING KINETICS, FOURIER-TRANSFORM INFRARED SPECTROSCOPY ANALYSIS AND SENSORY EVALUATION OF SUN, HOT-AIR, MICROWAVE AND FREEZE DRIED MANGO LEATHER 晒干、热空气干燥、微波干燥和冻干芒果皮的干燥动力学、傅里叶变换红外光谱分析和感官评价

The Journal of Microbiology, Biotechnology and Food Sciences Pub Date : 2021-03-23 DOI: 10.15414/JMBFS.3313

Tanmay Sarkar

引用次数: 12

MICROARRAY-BASED ANALYSIS OF STAPHYLOCOCCUS AUREUS ISOLATES FROM NON-CLINICAL SOURCE 非临床来源金黄色葡萄球菌分离株的微阵列分析

The Journal of Microbiology, Biotechnology and Food Sciences Pub Date : 2021-03-23 DOI: 10.15414/JMBFS.3194

A. H. Momoh-Zekeri, J. Kwaga, M. Bello, A. Sackey, A. Larsen

{"title":"MICROARRAY-BASED ANALYSIS OF STAPHYLOCOCCUS AUREUS ISOLATES FROM NON-CLINICAL SOURCE","authors":"A. H. Momoh-Zekeri, J. Kwaga, M. Bello, A. Sackey, A. Larsen","doi":"10.15414/JMBFS.3194","DOIUrl":"https://doi.org/10.15414/JMBFS.3194","url":null,"abstract":"This study was aimed to genotyped virulence, toxin and resistance genes of 30 S. aureus isolates from pigs using DNA microarray. The 30 isolates analyzed in this study all belonged to clonal complexes (CC) CC1 (3; 10%), CC5 (3; 10%), CC15 (16; 53.33%) and CC152 (8; 26.66%), respectively. Of antibiotic resistance-associated genes reported; Twenty out of 30 isolates (66.66%) carried the beta-lactamase operon (blaZ/I/R) and the tetracycline resistance gene (tetK), respectively. Also, the macrolide resistance gene (ermA/msrA) were detected in ten isolates (33.33%), the multidrug efflux pump resistance gene (sdrM) was detected in seven isolates (23.33%) and one isolates (3.33%) harboured the chloramphenicol resistance gene (cat). None of the isolates harboured genes conferring methicillin resistance. In terms of genes encoding enterotoxins; seb was detected among all isolates of CC15 while the enterotoxin gene cluster egc and a distinct variant of the enterotoxin A (sea-N315) were detected among isolates of CC5 and CC152, respectively. Two CC152 isolate was positive for genes encoding the Panton-Valentine leukocidin (lukF-PV+lukS-PV). In addition, all the isolates harboured the genes encoding for intracellular adhesion proteins (icaA/C/D) and the biofilm-associated genes (clfA and clfB) except for CC1 isolates. The capsular genes cap5 and cap8 were detected in isolates of CC5 and CC15 while only cap5 was present in CC152 isolates. The study provides detailed genotyping data on the population structure, presence of toxins and antibiotic resistance markers of S. aureus isolates and indicates the importance of the microarray technique in analyzing multiple gene contents of bacteria.","PeriodicalId":22746,"journal":{"name":"The Journal of Microbiology, Biotechnology and Food Sciences","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2021-03-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86649251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0