EFFECT OF DIETARY SUPPLEMENTATION WITH SEAWEED AND POLYPHENOLS MIXTURE ON ANTIOXIDANT STATUS, CONCENTRATION AND MOTILITY OF RABBIT SPERMATOZOA

N. Knížatová, M. Massányi, K. Tokárová, F. Vizzarri, P. Massányi, Ľ. Ondruška, N. Lukáč
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引用次数: 1

Abstract

In recent years, many studies have been focused on natural substances that could have effect on health of animals. We investigated effect of extract consisting mainly of polyphenols, brown algae and plant polysaccharides on the reproduction potential of male rabbits (New Zealand white breed) during 60 days long dietary experiment. The rabbits were divided into three groups. Control was fed a basal diet, whereas the second and third group were supplemented with seaweed and polyphenols mixture: T1 = 0.3% and T2 = 0.6% respectively. We observed that sperm concentration in both experimental groups increased in comparison to the control group. Results of the CASA analysis showed enhanced motility (C = 85.09±7.53%, T1 = 87.21±8.25%, T2 = 89.38±8.02%) and progressive motility (C = 74.28±12.6%, T1 = 79.07±13.89%, T2 = 81.28±11.37%) in experimental groups supplemented with combination of algae and polyphenols in comparison with the control group. While monitoring ferric reducing ability of plasma (FRAP), we found the highest value in T1 group, changes were insignificat. An increase in GPx activity was measured in experimental groups in comparison with the control group with major difference in T1 group. In experimental groups, we determined an increase in activity of superoxide dismutase (SOD) in comparison with control group, the difference was significant in T2 group (C = 0.26±0.11 U/mg TP; T2 = 0.31±0.09 U/mg TP). In conclusion, our studies suggest that dietary supplementation with brown seaweed and plant polyphenols mixture may be potentially useful for enhancement of sperm motility and protection against oxidative stress.
饲粮中添加海藻和多酚混合物对兔精子抗氧化状态、浓度和活力的影响
近年来,许多研究都集中在可能影响动物健康的天然物质上。在为期60 d的饲粮试验中,研究了以多酚、褐藻和植物多糖为主要成分的提取物对新西兰白兔繁殖能力的影响。兔子被分成三组。对照组饲喂基础饲粮,第二组和第三组在基础饲粮中添加海藻和多酚混合物,T1 = 0.3%, T2 = 0.6%。我们观察到,与对照组相比,两个实验组的精子浓度都有所增加。CASA分析结果显示,与对照组相比,藻多酚联合添加组小鼠运动能力增强(C = 85.09±7.53%,T1 = 87.21±8.25%,T2 = 89.38±8.02%),运动能力渐进式增强(C = 74.28±12.6%,T1 = 79.07±13.89%,T2 = 81.28±11.37%)。在监测血浆铁还原能力(FRAP)时,我们发现T1组最高,变化不显著。实验组GPx活性均较对照组升高,T1组差异较大。各实验组超氧化物歧化酶(SOD)活性较对照组升高,T2组差异有统计学意义(C = 0.26±0.11 U/mg TP;T2 = 0.31±0.09 U/mg TP)。总之,我们的研究表明,膳食中添加褐藻和植物多酚混合物可能有助于增强精子活力和抗氧化应激。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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