The Journal of applied bacteriology最新文献_第2页

Inhibitory effects of sucrose monolaurate, alone and in combination with organic acids, on Listeria monocytogenes and Staphylococcus aureus. 单月桂酸蔗糖单独和与有机酸联合对单核增生李斯特菌和金黄色葡萄球菌的抑制作用。

The Journal of applied bacteriology Pub Date : 1996-07-01 DOI: 10.1111/j.1365-2672.1996.tb03276.x

J D Monk, L R Beuchat, A K Hathcox

{"title":"Inhibitory effects of sucrose monolaurate, alone and in combination with organic acids, on Listeria monocytogenes and Staphylococcus aureus.","authors":"J D Monk, L R Beuchat, A K Hathcox","doi":"10.1111/j.1365-2672.1996.tb03276.x","DOIUrl":"https://doi.org/10.1111/j.1365-2672.1996.tb03276.x","url":null,"abstract":"The effects of sucrose esters of fatty acids, alone and in combination with ethylenediaminetetraacetic acid (EDTA), acetic acid, lactic acid and citric acid, on survival, growth and thermal inactivation of Listeria monocytogenes and Staphylococcus aureus were determined. The presence of sucrose monocaprate (400 micrograms ml-1) in tryptose phosphate broth (TPB) or tryptic soy broth (TSB) did not inhibit the growth of L. monocytogenes or Staph. aureus, respectively. However, significantly (P < or = 0.05) lower populations of L. monocytogenes were detected in TPB containing as little as 100 micrograms ml-1 sucrose monolaurate (SML) during the logarithmic growth phase compared to populations detected in TPB containing no SML. At 400 micrograms ml-1, SML was lethal to L. monocytogenes. Less marked inhibitory effects were observed with Staph. aureus. The addition of EDTA to broth containing SML had a synergistic effect on the inhibition of both organisms. The chelator alone had no effect at 100 micrograms ml-1 on either pathogen but was inhibitory at 200 micrograms ml-1. Inhibition of L. monocytogenes was more pronounced as the incubation temperature was decreased from 30 degrees C to 15 or 5 degrees C. The addition of 0.1% acetic or lactic acid to TPB minimized the inhibitory effect of 100 and 200 micrograms ml-1 SML during the first 32 h of incubation. Staphylococcus aureus behaved similarly, but not as dramatically, to L. monocytogenes when cultured in TSB supplemented with SML alone or SML and organic acids. A synergistic inhibitory effect of SML and EDTA on heat inactivation of L. monocytogenes was evident but the reverse phenomenon was observed with Staph. aureus. The effectiveness of SML in controlling the growth of L. monocytogenes and Staph. aureus in foods most likely to be contaminated with these pathogens should be further investigated.","PeriodicalId":22599,"journal":{"name":"The Journal of applied bacteriology","volume":"81 1","pages":"7-18"},"PeriodicalIF":0.0,"publicationDate":"1996-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1365-2672.1996.tb03276.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19651176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 49

Microbial biomass in a shallow, urban aquifer contaminated with aromatic hydrocarbons: analysis by phospholipid fatty acid content and composition. 被芳烃污染的浅层城市含水层微生物生物量:磷脂脂肪酸含量和组成分析。

The Journal of applied bacteriology Pub Date : 1996-06-01 DOI: 10.1111/j.1365-2672.1996.tb03266.x

P D Franzmann, B M Patterson, T R Power, P D Nichols, G B Davis

{"title":"Microbial biomass in a shallow, urban aquifer contaminated with aromatic hydrocarbons: analysis by phospholipid fatty acid content and composition.","authors":"P D Franzmann, B M Patterson, T R Power, P D Nichols, G B Davis","doi":"10.1111/j.1365-2672.1996.tb03266.x","DOIUrl":"https://doi.org/10.1111/j.1365-2672.1996.tb03266.x","url":null,"abstract":"The city of Perth contains a number of sites that have been contaminated with hydrocarbons due to leakage from petroleum underground storage tanks. Microbial biomass in groundwater and sediment cores from above and below the water table, and from within and outside a plume of hydrocarbon contamination, was examined using phospholipid fatty acid methyl ester analysis. Microbial numbers, calculated from the phospholipid content, ranged from 0.9 x 10(6) to 7.8 x 10(6) 'Escherichia coli equivalent cells' g-1 dry wt of sediment. Over 96% of the microbial biomass was attached to the sediment and the proportion of attached cells did not decrease within the plume of contaminants. The amount of biomass within aquifer samples seemed to be related more to the proximity of the rhizosphere to the shallow aquifer, and other unknown urban inputs, rather than to the effects of the plume of contaminants. Fatty acids common to many bacterial groups dominated within the plume, and as such the analyses gave limited insight into microbial community structure. For site assessment of intrinsic remediation of shallow aquifers in urban areas, estimates of microbial biomass may not provide information that is readily applicable to plume management.","PeriodicalId":22599,"journal":{"name":"The Journal of applied bacteriology","volume":"80 6","pages":"617-25"},"PeriodicalIF":0.0,"publicationDate":"1996-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1365-2672.1996.tb03266.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19672713","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 45

Pediocin 5 production and plasmid stability during continuous free and immobilized cell cultures of Pediococcus acidilactici UL5. 酸性化Pediococcus acilactii UL5连续游离和固定化细胞培养中Pediocin 5的产生和质粒稳定性

The Journal of applied bacteriology Pub Date : 1996-06-01 DOI: 10.1111/j.1365-2672.1996.tb03268.x

J Huang, C Lacroix, H Daba, R E Simard

{"title":"Pediocin 5 production and plasmid stability during continuous free and immobilized cell cultures of Pediococcus acidilactici UL5.","authors":"J Huang, C Lacroix, H Daba, R E Simard","doi":"10.1111/j.1365-2672.1996.tb03268.x","DOIUrl":"https://doi.org/10.1111/j.1365-2672.1996.tb03268.x","url":null,"abstract":"Continuous production of pediocin 5 from Pediococcus acidilactici UL5 was investigated in MRS medium at different pH and dilution rates during continuous free cell (FC) and immobilized cell (IC) fermentations. Pediocin 5 activity from FC operated at a dilution rate of 0.31 h-1 largely increased from 128 to 2048 AU mL-1 as pH decreased from 7.0 to 5.0. Pediocin 5 activity in IC at a dilution rate of 0.93 h-1 was much less affected by pH, varying from 256 AU mL-1 at pH 7.0 to 512 AU mL-1 at pH 5.0. At the optimum pH 5.0, the dilution rate greatly influenced pediocin 5 activity both in FC and IC. Pediocin 5 production during continuous FC culture decreased with time for all dilution rates tested except 0.31 h-1 and average activity over 144 h cultures reached a maximal value of 4915 AU mL-1 at a dilution rate of 0.26 h-1. For IC, pediocin 5 production was stable with time and increased with the dilution rate from 256 to 1024 AU mL-1 in the range of 0.47-2.28 h-1. Three Listeria strains were tested for their ability to screen low bacteriocin-producing variants (Bac+v) of Bac+ cells in FC and IC cultures by using a modified deferred antagonism method. Ten to 28% of Bac+v cells appeared after 144 h of FC cultures at dilution rates in the range 0.09-0.42 h-1 and pH control set points of 5.0-7.0 while almost no Bac+v cell was detected during 192 h IC culture in the same pH range and for dilution rates varying from 0.47 to 2.28 h-1. The Bac+v cells isolated produced eight- to 64-fold less pediocin 5 than the Bac+ cells. Although electrophoresis analysis showed no apparent difference in the plasmid profiles of Bac+v and Bac+ cells, the Bac- mutant obtained by acriflavine treatment had lost the pMJ5 plasmid encoding for bacteriocin production. The decreased quantity of plasmid DNA in Bac+v cells suggests that the decreased pediocin 5 activity of Bac+v cells resulted from a decrease in plasmid copy number.","PeriodicalId":22599,"journal":{"name":"The Journal of applied bacteriology","volume":"80 6","pages":"635-44"},"PeriodicalIF":0.0,"publicationDate":"1996-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1365-2672.1996.tb03268.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19672715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 43

Detection of Nitrosococcus oceanus in a Mediterranean lagoon by immunofluorescence. 免疫荧光法检测地中海泻湖中海洋亚硝基球菌。

The Journal of applied bacteriology Pub Date : 1996-06-01 DOI: 10.1111/j.1365-2672.1996.tb03265.x

R Zaccone, G Caruso, M Azzaro

引用次数: 16

Differentiation between Mycobacterium tuberculosis and Mycobacterium bovis by a multiplex-polymerase chain reaction. 多重聚合酶链反应对结核分枝杆菌和牛分枝杆菌的鉴别。

The Journal of applied bacteriology Pub Date : 1996-06-01 DOI: 10.1111/j.1365-2672.1996.tb03263.x

E A Herrera, O Pérez, M Segovia

引用次数: 20

Regulation of the nisin operons in Lactococcus lactis N8. 乳酸乳球菌N8中乳杆菌蛋白操纵子的调控。

The Journal of applied bacteriology Pub Date : 1996-06-01 DOI: 10.1111/j.1365-2672.1996.tb03267.x

M Qiao, S Ye, O Koponen, R Ra, M Usabiaga, T Immonen, P E Saris

{"title":"Regulation of the nisin operons in Lactococcus lactis N8.","authors":"M Qiao, S Ye, O Koponen, R Ra, M Usabiaga, T Immonen, P E Saris","doi":"10.1111/j.1365-2672.1996.tb03267.x","DOIUrl":"https://doi.org/10.1111/j.1365-2672.1996.tb03267.x","url":null,"abstract":"The antibiotic peptide nisin produced by Lactococcus lactis is used as a food preservative due to its activity against spores and vegetative cells of Gram-positive bacteria. The post-translational maturation of this secreted peptide includes dehydration of serine and threonine residues, lanthionine formation and a proteolytic processing of 23 amino acids from the N-terminus. Mutations in the nisZ, nisB and nisP genes of the biosynthetic nisZBTCIPRK nisin operon were made by gene replacement or integration of a plasmid. The mutations caused a drastic decrease of the transcription from the promoters upstream of the nisZBTCIPRK and nisFEG operons resulting in loss of nisin production and nisin immunity. The transcription of the nisin operons and nisin immunity could be partially restored by adding nisin to the growth medium of the cells. Nisin induction of the mutant strains also increased the level of the putative immunity NisI protein. These results showed that the nisZBTCIPRK operon is positively autoregulated and that the nisFEG operon is in the same regulon.","PeriodicalId":22599,"journal":{"name":"The Journal of applied bacteriology","volume":"80 6","pages":"626-34"},"PeriodicalIF":0.0,"publicationDate":"1996-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1365-2672.1996.tb03267.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19672714","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 58

The use of an automated assay to assess phage survival after a biocidal treatment. 使用一种自动测定法来评估杀菌剂处理后噬菌体的存活率。

The Journal of applied bacteriology Pub Date : 1996-06-01 DOI: 10.1111/j.1365-2672.1996.tb03264.x

J Y Maillard, T S Beggs, M J Day, R A Hudson, A D Russell

引用次数: 7

Quantification of Staphylococcus aureus and Escherichia coli in the liquid medium by fluorimetry and its use in phagocytosis assay. 液体培养基中金黄色葡萄球菌和大肠杆菌的荧光定量及其在吞噬试验中的应用。

The Journal of applied bacteriology Pub Date : 1996-06-01 DOI: 10.1111/j.1365-2672.1996.tb03260.x

W Fang

引用次数: 6

Interaction between fish spoilage bacteria Pseudomonas sp. and Shewanella putrefaciens in fish extracts and on fish tissue. 鱼类腐坏细菌假单胞菌和腐烂希瓦氏菌在鱼提取物和鱼组织中的相互作用。

The Journal of applied bacteriology Pub Date : 1996-06-01 DOI: 10.1111/j.1365-2672.1996.tb03262.x

L Gram, J Melchiorsen

{"title":"Interaction between fish spoilage bacteria Pseudomonas sp. and Shewanella putrefaciens in fish extracts and on fish tissue.","authors":"L Gram, J Melchiorsen","doi":"10.1111/j.1365-2672.1996.tb03262.x","DOIUrl":"https://doi.org/10.1111/j.1365-2672.1996.tb03262.x","url":null,"abstract":"The interaction between fish spoilage bacteria, Pseudomonas sp. and Shewanella putrefaciens, was investigated using fish extract and fish tissue as model systems. Isolates of Pseudomonas that produced iron chelators, siderophores, inhibited growth of S. putrefaciens in a fish-extract-agar diffusion assay but no, or only weak, antagonistic activity was seen when the medium was supplemented with iron. Sterile-filtered supernatant fluid from a siderophore-producing Pseudomonas grown in fish extract was inhibitory to S. putrefaciens if the number of Psudomonas was above 10(8) cfu ml-1. In contrast, supernatant fluids from siderophore-negative Pseudomonas isolates did not inhibit growth of S. putrefaciens. The inhibitory effect was, except for one strain of Pseudomonas, not seen in supernatant fluids from iron-enriched cultures of Pseudomonas sp. Finally, siderophore-producing Pseudomonas sp. lowered the maximum cell level of S. putrefaciens 1-2 log units from 10(9) to 10(10) cfu g-1 when the strains were grown on fish muscle blocks at 0 degrees C but the growth rate of S. putrefaciens was not affected.","PeriodicalId":22599,"journal":{"name":"The Journal of applied bacteriology","volume":"80 6","pages":"589-95"},"PeriodicalIF":0.0,"publicationDate":"1996-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1365-2672.1996.tb03262.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19672812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 163

A comparison of a new campylobacter selective medium (CAT) with membrane filtration for the isolation of thermophilic campylobacters including Campylobacter upsaliensis. 新型弯曲菌选择培养基(CAT)与膜过滤分离嗜热弯曲菌(包括上萨利弯曲菌)的比较。

The Journal of applied bacteriology Pub Date : 1996-06-01 DOI: 10.1111/j.1365-2672.1996.tb03269.x

S T Aspinall, D R Wareing, P G Hayward, D N Hutchinson

{"title":"A comparison of a new campylobacter selective medium (CAT) with membrane filtration for the isolation of thermophilic campylobacters including Campylobacter upsaliensis.","authors":"S T Aspinall, D R Wareing, P G Hayward, D N Hutchinson","doi":"10.1111/j.1365-2672.1996.tb03269.x","DOIUrl":"https://doi.org/10.1111/j.1365-2672.1996.tb03269.x","url":null,"abstract":"The newly developed CAT campylobacter selective medium employing the blood-free charcoal-based agar containing cefoperazone (8 mg l-1), amphotericin (10 mg l-1) and teicoplanin (4 mg l-1) was compared with the membrane filtration culture technique for isolation of Campylobacter spp. including Camp. upsaliensis. Nine hundred and fifty human, 275 dog and 65 cat faeces (in which modified CCDA medium was also compared) were tested. In addition, the recovery of Camp. upsaliensis from pure cultures and from spiked human faeces was examined after membrane filtration. A 50-fold reduction in recovery after filtration using the 0.65 micron filters and a 150-fold reduction using the 0.45 micron filters was found. Recovery of Camp. upsaliensis from spiked faeces was considerably improved using the CAT medium compared with filtration, especially with the lower concentration of organisms (approx. 10(4) cfu mL-1). Campylobacter upsaliensis was recovered from 91 specimens of animal faeces, with CCDA recovering 26 isolates (29%), CAT recovering 76 isolates (84%) and membrane filtration (0.65 microns) recovering 82 isolates (90%). CAT selective agar was found to be a suitable medium for the isolation of thermophilic campylobacters including Camp. upsaliensis from faecal samples.","PeriodicalId":22599,"journal":{"name":"The Journal of applied bacteriology","volume":"80 6","pages":"645-50"},"PeriodicalIF":0.0,"publicationDate":"1996-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1365-2672.1996.tb03269.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19672716","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 45