使用一种自动测定法来评估杀菌剂处理后噬菌体的存活率。

J Y Maillard, T S Beggs, M J Day, R A Hudson, A D Russell
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引用次数: 7

摘要

噬菌体K1-5已被用于自动检测各种消毒剂的杀病毒活性。这种基于宿主细胞(大肠杆菌D837)裂解的分光光度读数的间接测定产生了令人鼓舞的结果,并且比依赖斑块形成的覆盖计数法(先前研究过)更快。但两种方法对某些消毒剂的敏感性存在差异。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The use of an automated assay to assess phage survival after a biocidal treatment.

The coliphage K1-5 has been used in an automated assay to monitor the viricidal activity of various disinfectants. This indirect assay based on the spectrophotometric reading of the lysis of the host cell (Escherichia coli D837) produced encouraging results and was faster than the overlay counting method (previously studied) which relies on plaque formation. However, differences in sensitivity towards some disinfectants were observed between the two methods.

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