The Journal of applied bacteriology最新文献_第3页

Biological indicators for low temperature steam and formaldehyde sterilization: investigation of the effect of change in temperature and formaldehyde concentration on spores of Bacillus stearothermophilus NCIMB 8224. 低温蒸汽甲醛灭菌生物学指标:温度和甲醛浓度变化对嗜热脂肪芽孢杆菌NCIMB 8224孢子影响的研究

The Journal of applied bacteriology Pub Date : 1996-03-01 DOI: 10.1111/j.1365-2672.1996.tb03218.x

A M Wright, E V Hoxey, C J Soper, D J Davies

引用次数: 0

Impedance microbiology--a rapid change for microbiologists. 阻抗微生物学——微生物学家的一个快速变化。

The Journal of applied bacteriology Pub Date : 1996-03-01 DOI: 10.1111/j.1365-2672.1996.tb03215.x

P Silley, S Forsythe

引用次数: 190

Pulsed-field gel electrophoresis as an epidemiological tool for clonal identification of Aeromonas hydrophila. 脉冲场凝胶电泳技术在嗜水气单胞菌克隆鉴定中的应用。

The Journal of applied bacteriology Pub Date : 1996-03-01 DOI: 10.1111/j.1365-2672.1996.tb03220.x

D Talon, M J Dupont, J Lesne, M Thouverez, Y Michel-Briand

{"title":"Pulsed-field gel electrophoresis as an epidemiological tool for clonal identification of Aeromonas hydrophila.","authors":"D Talon, M J Dupont, J Lesne, M Thouverez, Y Michel-Briand","doi":"10.1111/j.1365-2672.1996.tb03220.x","DOIUrl":"https://doi.org/10.1111/j.1365-2672.1996.tb03220.x","url":null,"abstract":"Pulsed-field gel electrophoresis (PFGE) was used to characterize Aeromonas hydrophila strains isolated from a cluster of hospital-acquired infections that occurred over approximately 1 month in a French hospital. Five isolates from patients and 10 isolates from the water supply were characterized by biotyping and antibiotic susceptibility patterns and compared with 10 epidemiologically unrelated strains isolated from patients and rivers, by PFGE of digests of chromosomal DNA. Five environmental and four clinical isolates belonged to the same biotype and antibiotic susceptibility pattern type. The endonucleases XbaI, SpeI and SwaI gave satisfactory profiles whereas DraI did not. The profiles were stable, reproducible and discriminatory. The 10 epidemiologically unrelated strains exhibited 10 different patterns after digestion with XbaI, the least expensive, suitable endonuclease. PFGE is a rapid and discriminatory technique for the typing of Aeromonas hydrophila where a common origin of infection is suspected.","PeriodicalId":22599,"journal":{"name":"The Journal of applied bacteriology","volume":"80 3","pages":"277-82"},"PeriodicalIF":0.0,"publicationDate":"1996-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1365-2672.1996.tb03220.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19819578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 59

The application of a log-logistic model to describe the thermal inactivation of Clostridium botulinum 213B at temperatures below 121.1 degrees C. 应用logistic模型描述肉毒杆菌213B在温度低于121.1℃时的热失活。

The Journal of applied bacteriology Pub Date : 1996-03-01 DOI: 10.1111/j.1365-2672.1996.tb03221.x

W A Anderson, P J McClure, A C Baird-Parker, M B Cole

引用次数: 110

Alkaline phosphatase activity of Escherichia coli starved in sterile lake water microcosms. 无菌湖水中大肠杆菌碱性磷酸酶活性的研究。

The Journal of applied bacteriology Pub Date : 1996-03-01 DOI: 10.1111/j.1365-2672.1996.tb03217.x

R Ozkanca, K P Flint

引用次数: 12

Survival potential of Aeromonas hydrophila in freshwaters and nutrient-poor waters in comparison with other bacteria. 与其他细菌相比，嗜水气单胞菌在淡水和贫营养水中的生存潜力。

The Journal of applied bacteriology Pub Date : 1996-03-01 DOI: 10.1111/j.1365-2672.1996.tb03219.x

I Kersters, G Huys, H Van Duffel, M Vancanneyt, K Kersters, W Verstraete

{"title":"Survival potential of Aeromonas hydrophila in freshwaters and nutrient-poor waters in comparison with other bacteria.","authors":"I Kersters, G Huys, H Van Duffel, M Vancanneyt, K Kersters, W Verstraete","doi":"10.1111/j.1365-2672.1996.tb03219.x","DOIUrl":"https://doi.org/10.1111/j.1365-2672.1996.tb03219.x","url":null,"abstract":"The survival of a genetically-marked Aeromonas hydrophila strain was studied in water microcosms using viable counts. Aeromonas hydrophila AWWX1 was shown to survive without decline in viable counts for at least 10 d in three of four filtered-autoclaved freshwaters (surface water and groundwater) and in all examined filtered-autoclaved nutrient-poor waters (bottled spring water, Milli-Q and tap water). However, in the unfiltered waters, a rapid decrease in viable counts of Aer. hydrophila AWWX1 was observed after 1-5 d. The survival of Aer. hydrophila AWWX1 in nutrient-poor waters was compared with that of Pseudomonas fluorescens P17 and Spirillum strain NOX. Survival characteristics were organism- and water-dependent. In the filtered-autoclaved waters, viable counts of Spirillum strain NOX were ca 1 log-unit higher than for Aer. hydrophila AWWX1 and Ps. fluorescens P17. The tested strains Aer. hydrophila AWWX1 and Ps. fluorescens P17 survived 3 to 20, respectively 2 to 4 times better in the filtered-autoclaved waters compared to the unfiltered waters. Apparently, any inherent capability of these micro-organisms to adapt to low-nutrient environments was undone by the presence of the autochthonous microbiota. The present findings that Aer. hydrophila survives very poorly in several drinking waters is of utmost importance towards public health and arises questions about the mechanisms involved.","PeriodicalId":22599,"journal":{"name":"The Journal of applied bacteriology","volume":"80 3","pages":"266-76"},"PeriodicalIF":0.0,"publicationDate":"1996-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1365-2672.1996.tb03219.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19819577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 65

Detection of Salmonella typhi by polymerase chain reaction. 聚合酶链反应检测伤寒沙门菌。

The Journal of applied bacteriology Pub Date : 1996-03-01 DOI: 10.1111/j.1365-2672.1996.tb03216.x

Q Zhu, C K Lim, Y N Chan

{"title":"Detection of Salmonella typhi by polymerase chain reaction.","authors":"Q Zhu, C K Lim, Y N Chan","doi":"10.1111/j.1365-2672.1996.tb03216.x","DOIUrl":"https://doi.org/10.1111/j.1365-2672.1996.tb03216.x","url":null,"abstract":"A rapid and sensitive method for detection of Salmonella typhi would help in preventing the spread of outbreaks and in clinical diagnosis. In order to develop unique PCR primers to detect Salm. typhi, ribosomal RNA genes from Salm. typhi (Rawlings) were cloned in pUC18. The resulting clone was confirmed by sequencing. The cloned DNA fragment contained the 5S, part of the 23S rRNA genes and the 5S-23S spacer region (EMBL/GenBank accession No. U04734). It was expected that the 5S-23S spacer region is divergent unlike the highly conserved 23S + 5S genes. This was confirmed by comparison with the rRNA gene sequences in the EMBL/GenBank database. A pair of PCR primers specific for Salm. typhi was obtained, based on this spacer region sequence. The specificity of this pair of primers was tested with 54 Salm. typhi strains (of 27 different phage types). All these Salm. typhi strains showed the positive 300 bp PCR product with this pair of primers. Six other Salmonella species as well as six other non-Salmonella bacteria were tested and none showed the 300 bp PCR product. The sensitivity of the detection level was 0.1 pg of pure Salm. typhi genomic DNA, or approximately 40 Salm. typhi cells in a spiked food sample. This pair of primers therefore has the potential for development into a diagnostic tool for the rapid diagnosis of typhoid fever.","PeriodicalId":22599,"journal":{"name":"The Journal of applied bacteriology","volume":"80 3","pages":"244-51"},"PeriodicalIF":0.0,"publicationDate":"1996-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1365-2672.1996.tb03216.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19819677","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 81

65th Annual meeting of the Society for Applied Bacteriology summer conference. 15-18 July 1996, United Kingdom. Abstracts. 第65届应用细菌学学会夏季年会。1996年7月15日至18日，联合王国。摘要。

The Journal of applied bacteriology Pub Date : 1996-01-01

引用次数: 0

Random amplified polymorphic DNA and restriction enzyme analysis of PCR amplified rDNA in taxonomy: two identification techniques for food-borne yeasts. 随机扩增多态性DNA和PCR扩增rDNA的限制性内切酶分析:食源性酵母的两种鉴定技术。

The Journal of applied bacteriology Pub Date : 1995-11-01 DOI: 10.1111/j.1365-2672.1995.tb03173.x

M M Baleiras Couto, J T Vogels, H Hofstra, J H Huis in't Veld, J M van der Vossen

{"title":"Random amplified polymorphic DNA and restriction enzyme analysis of PCR amplified rDNA in taxonomy: two identification techniques for food-borne yeasts.","authors":"M M Baleiras Couto, J T Vogels, H Hofstra, J H Huis in't Veld, J M van der Vossen","doi":"10.1111/j.1365-2672.1995.tb03173.x","DOIUrl":"https://doi.org/10.1111/j.1365-2672.1995.tb03173.x","url":null,"abstract":"The random amplified polymorphic DNA (RAPD) assay and the restriction enzyme analysis of PCR amplified rDNA are compared for the identification of the common spoilage yeasts Zygosaccharomyces bailii, Z. rouxii, Saccharomyces cerevisiae, Candida valida and C. lipolytica. Both techniques proved to be adequate tools for yeast identification. Since the RAPD does provide less stable patterns than restriction enzyme analysis of PCR amplified rDNA, and a large amount of data had to be compared without data reduction, Principal Component Analysis (PCA) was applied successfully for clustering the RAPD patterns. The success of PCA is highly influenced by the primer used in RAPD and the amount of reference samples. A large amount of reference samples improves the performance of clustering in PCA. The primer of choice was shown to be important with respect to the discriminatory power of the RAPD method. Some primers used enabled discrimination on the subspecies level. The results collected with both typing methods justify the conclusion that the present typing system can be applied for taxonomical purposes.","PeriodicalId":22599,"journal":{"name":"The Journal of applied bacteriology","volume":"79 5","pages":"525-35"},"PeriodicalIF":0.0,"publicationDate":"1995-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1365-2672.1995.tb03173.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19547544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 69

Assessment of biological activity and fate of organic compounds in a reactor for the measurement of biodegradable organic carbon in water. 测定水中可生物降解有机碳的反应器中有机化合物的生物活性和归宿的评价。

The Journal of applied bacteriology Pub Date : 1995-11-01 DOI: 10.1111/j.1365-2672.1995.tb03177.x

F Ribas, J Frías, F Ventura, L Mohedano, F Lucena

{"title":"Assessment of biological activity and fate of organic compounds in a reactor for the measurement of biodegradable organic carbon in water.","authors":"F Ribas, J Frías, F Ventura, L Mohedano, F Lucena","doi":"10.1111/j.1365-2672.1995.tb03177.x","DOIUrl":"https://doi.org/10.1111/j.1365-2672.1995.tb03177.x","url":null,"abstract":"A new, rapid method for the determination of biodegradable dissolved organic carbon (BDOC), especially suited to water industry needs, was recently proposed by the authors. This dynamic method measured the BDOC of circulating water continuously pumped over a biofilm attached to a special support (sinterized porous glass) that fills a system of two glass columns. The BDOC value corresponds to the difference in dissolved organic carbon (DOC) between inflow and outflow water samples. The analytical results are not significantly different from those of other bioassays that use indigenous bacteria, and the total duration of the analysis is less than 3 h. However, a problem common to all the BDOC methods based on attached bacteria is the extent to which the decrease in DOC during the BDOC analysis is due to true biodegradation or to adsorption of organic matter to the reactor. In the present study, a reasonable support is provided for the hypothesis that this decrease, at least in the dynamic method, is predominantly due to microbiological activity. After comparing the support (sinterized porous glass) with a good physical adsorbent (granular activated carbon), the influence of temperature, residual chlorine and sodium azide on the reactor performance was tested, and a sensitivity only attributable to biological activity was observed. Another set of experiments were performed to assess the fate and specific elimination of different organic substances, explicable assuming that biodegradation processes were involved.","PeriodicalId":22599,"journal":{"name":"The Journal of applied bacteriology","volume":"79 5","pages":"558-68"},"PeriodicalIF":0.0,"publicationDate":"1995-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1111/j.1365-2672.1995.tb03177.x","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19547546","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11