液体培养基中金黄色葡萄球菌和大肠杆菌的荧光定量及其在吞噬试验中的应用。

W Fang
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引用次数: 6

摘要

用荧光法和平板计数法对液体培养基中金黄色葡萄球菌和大肠杆菌的活菌量进行了比较。荧光法测定了细菌磷酸酶从4-甲基伞形草酰磷酸中释放的荧光性4-甲基伞形草酮(4-MU)。荧光的增加与接种菌的大小有关。将荧光阈值设置在对数生长期的中间,两种葡萄球菌的荧光与细菌数量呈良好的线性关系(r = 0.99)。金黄色葡萄球菌和大肠杆菌)。荧光法和平板计数法在定量生理盐水中活菌方面也有很好的相关性(r = 0.99)。进一步比较两种方法对吞噬后细胞外细菌的评价。一般来说,荧光技术对大肠杆菌的吞噬率比平板计数法高,具有统计学意义。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Quantification of Staphylococcus aureus and Escherichia coli in the liquid medium by fluorimetry and its use in phagocytosis assay.

A fluorimetric technique was compared with the plate counting method for quantification of viable cells of Staphylococcus aureus and Escherichia coli in the liquid medium. The fluorimetric assay measures the release of fluorogenic 4-methylumbelliferone (4-MU) from 4-methylumbelliferyl phosphate by the bacterial phosphatases. The increase in fluorescence was dependent on the size of bacterial inocula. Setting the fluorescence threshold at the middle of the logarithmic growth phase resulted in good linear relationship between bacterial counts and fluorescence (r = 0.99 for both Staph. aureus and E. coli). There was also an excellent correlation between the fluorimetric assay and the plate counting method in quantifying viable bacteria in saline (r = 0.99). Both methods were further compared for evaluation of extracellular bacteria following phagocytosis. The fluorimetric technique, in general, gave a higher percentage of phagocytosis than the plate counting method with statistical significance for E. coli.

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