A comparison of a new campylobacter selective medium (CAT) with membrane filtration for the isolation of thermophilic campylobacters including Campylobacter upsaliensis.

S T Aspinall, D R Wareing, P G Hayward, D N Hutchinson
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引用次数: 45

Abstract

The newly developed CAT campylobacter selective medium employing the blood-free charcoal-based agar containing cefoperazone (8 mg l-1), amphotericin (10 mg l-1) and teicoplanin (4 mg l-1) was compared with the membrane filtration culture technique for isolation of Campylobacter spp. including Camp. upsaliensis. Nine hundred and fifty human, 275 dog and 65 cat faeces (in which modified CCDA medium was also compared) were tested. In addition, the recovery of Camp. upsaliensis from pure cultures and from spiked human faeces was examined after membrane filtration. A 50-fold reduction in recovery after filtration using the 0.65 micron filters and a 150-fold reduction using the 0.45 micron filters was found. Recovery of Camp. upsaliensis from spiked faeces was considerably improved using the CAT medium compared with filtration, especially with the lower concentration of organisms (approx. 10(4) cfu mL-1). Campylobacter upsaliensis was recovered from 91 specimens of animal faeces, with CCDA recovering 26 isolates (29%), CAT recovering 76 isolates (84%) and membrane filtration (0.65 microns) recovering 82 isolates (90%). CAT selective agar was found to be a suitable medium for the isolation of thermophilic campylobacters including Camp. upsaliensis from faecal samples.

新型弯曲菌选择培养基(CAT)与膜过滤分离嗜热弯曲菌(包括上萨利弯曲菌)的比较。
采用含头孢哌酮(8 mg l-1)、两性霉素(10 mg l-1)和替柯planin (4 mg l-1)的无血炭基琼脂制备的CAT弯曲杆菌选择性培养基,与膜过滤培养技术分离Camp等弯曲杆菌进行了比较。海欧。测试了950份人类粪便、275份狗粪便和65份猫粪便(其中也比较了改良的CCDA培养基)。此外,还恢复了营地。用膜过滤法检测纯培养物和人粪便中的Upsaliensis。使用0.65微米过滤器过滤后,回收率降低了50倍,使用0.45微米过滤器过滤后回收率降低了150倍。营地的恢复。与过滤相比,使用CAT培养基可显著改善加刺粪便中的上saliensis,特别是在微生物浓度较低的情况下。10(4) cfu mL-1)。从91份动物粪便中检出上saliensis弯曲杆菌,其中CCDA检出26株(29%),CAT检出76株(84%),膜过滤(0.65微米)检出82株(90%)。CAT选择琼脂是一种适合分离包括Camp在内的嗜热弯曲杆菌的培养基。从粪便样本中发现Upsaliensis。
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