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The International journal of biochemistry最新文献

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Purification and characterization of alpha 1-thiol proteinase inhibitor and its identity with kinin- and fragment 1.2-free high molecular weight kininogen. α - 1-巯基蛋白酶抑制剂的纯化、鉴定及其与激肽和无片段1.2高分子量激肽原的同源性。
The International journal of biochemistry Pub Date : 1988-01-01 DOI: 10.1016/0020-711x(88)90348-5
I Ohkubo, C Namikawa, S Higashiyama, M Sasaki, O Minowa, Y Mizuno, H Shiokawa
{"title":"Purification and characterization of alpha 1-thiol proteinase inhibitor and its identity with kinin- and fragment 1.2-free high molecular weight kininogen.","authors":"I Ohkubo,&nbsp;C Namikawa,&nbsp;S Higashiyama,&nbsp;M Sasaki,&nbsp;O Minowa,&nbsp;Y Mizuno,&nbsp;H Shiokawa","doi":"10.1016/0020-711x(88)90348-5","DOIUrl":"https://doi.org/10.1016/0020-711x(88)90348-5","url":null,"abstract":"<p><p>1. alpha 1-Thiol proteinase inhibitor (alpha 1 TPI) purified from outdated human plasma was a glycoprotein with Mr 83,000 and was composed of heavy and light chains held together with a disulfide bond. 2. The data on amino acid composition, amino terminal sequence of the light chain and carboxyl terminal sequences of the heavy and light chains indicate that alpha 1 TPI is identical with kinin- and fragment 1.2-free HMW kininogen. 3. Purified human plasmin generated a derivative having the same molecular weight (Mr 83,000), same subunit structure (heavy and light chains) and same inhibitory capacity as alpha 1 TPI from HMW kininogen and kinin-free HMW kininogen. This indicated the possibility that alpha 1 TPI is derived from HMW kininogen by plasmin.</p>","PeriodicalId":22539,"journal":{"name":"The International journal of biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0020-711x(88)90348-5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14482758","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 19
The search for the DNA of the chick embryo fibroblast cytosolic complex. 鸡胚成纤维细胞胞质复合体DNA的寻找。
The International journal of biochemistry Pub Date : 1988-01-01 DOI: 10.1016/0020-711x(88)90350-3
C Challen, D H Adams
{"title":"The search for the DNA of the chick embryo fibroblast cytosolic complex.","authors":"C Challen,&nbsp;D H Adams","doi":"10.1016/0020-711x(88)90350-3","DOIUrl":"https://doi.org/10.1016/0020-711x(88)90350-3","url":null,"abstract":"<p><p>1. Conventional DNA extraction procedures have failed to release free DNA from the chick embryo fibroblast cytosolic DNA-RNA complexes. 2. Free DNA has been released only from the smallest cell cytosol DNA fraction, which is not in the native state associated with RNA: it is very small (of the order of 100 bases) and single stranded. 3. However, phenol extraction does separate complex DNA-associated material from the RNA which has invariably been found to accompany it in all but the smallest fraction (see 2 above). 4. The principal factor preventing DNA release appears to be a massive aggregation of partially purified DNA-associated material.</p>","PeriodicalId":22539,"journal":{"name":"The International journal of biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0020-711x(88)90350-3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13597311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 3
Characterization of rat parotid protein kinase C. 大鼠腮腺蛋白激酶C的特性。
The International journal of biochemistry Pub Date : 1988-01-01 DOI: 10.1016/0020-711x(88)90355-2
M T Hincke
{"title":"Characterization of rat parotid protein kinase C.","authors":"M T Hincke","doi":"10.1016/0020-711x(88)90355-2","DOIUrl":"https://doi.org/10.1016/0020-711x(88)90355-2","url":null,"abstract":"<p><p>1. Protein kinase C (PK-C) from the rat parotid gland has been partially purified and characterized for the first time. During its purification, this enzyme exhibited the same chromatographic behavior as the rat brain enzyme. 2. Affinities for phosphatidylserine (3 micrograms/ml), ATP (8 microM) and calcium (8 microM) were determined kinetically and found to be similar for the enzymes from each tissue. 3. Experiments designed to detect agonist-stimulated translocation of PK-C activity during phosphatidylinositol turnover found no change in levels of soluble PK-C, suggesting that PK-C translocation may not be an obligatory correlate of its activation. The implications of this result are discussed.</p>","PeriodicalId":22539,"journal":{"name":"The International journal of biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0020-711x(88)90355-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14481970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
Properties of sulfanilazo-haptoglobin. 磺胺偶氮-触珠蛋白的性质。
The International journal of biochemistry Pub Date : 1988-01-01 DOI: 10.1016/0020-711x(88)90358-8
W Dobryszycka, T Guszczyński
{"title":"Properties of sulfanilazo-haptoglobin.","authors":"W Dobryszycka,&nbsp;T Guszczyński","doi":"10.1016/0020-711x(88)90358-8","DOIUrl":"https://doi.org/10.1016/0020-711x(88)90358-8","url":null,"abstract":"<p><p>1. Tyrosine and two structural isomers of histidine residues in human haptoglobin were modified with diazotized sulfanilic acid. Sulfanilazo-derivatives of haptoglobin obtained by increasing the reagent/protein molar ration showed gradual decrease of peroxidase activity when complexed with hemoglobin. 2. Formation of haptoglobin derivatives with ten mono(sulfanilazo)-tyrosines and two mono (sulfanilazo)histidines resulted in the blockage of one out of six antigenic determinants, whereas immunoreactivity of the derivative with fourteen azotyrosines, one C-4, and two C-2 azohistidines was decreased by half. 3. Removal of sialic acid from oligosaccharide chains of haptoglobin made the molecule more accessible to diazotized sulfanilic acid. 4. Sulfanilazo-modification of tyrosine and histidine residues was practically of no effect in the reaction of haptoglobin with plant lectin, concanavalin A.</p>","PeriodicalId":22539,"journal":{"name":"The International journal of biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0020-711x(88)90358-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14481974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Polysaccharide and glycolipid composition in Tritrichomonas foetus. 毛滴虫胎儿的多糖和糖脂组成。
The International journal of biochemistry Pub Date : 1988-01-01 DOI: 10.1016/0020-711x(88)90360-6
B P Dias Filho, C S Alviano, W de Souza, J Angluster
{"title":"Polysaccharide and glycolipid composition in Tritrichomonas foetus.","authors":"B P Dias Filho,&nbsp;C S Alviano,&nbsp;W de Souza,&nbsp;J Angluster","doi":"10.1016/0020-711x(88)90360-6","DOIUrl":"https://doi.org/10.1016/0020-711x(88)90360-6","url":null,"abstract":"<p><p>1. The polysaccharide and glycolipid composition in Tritrichomonas foetus was studied by paper, thin-layer and gas-liquid chromatographic analysis. 2. The carbohydrate components of the polysaccharide were glucose (47%), galactose (34%) and mannose (19%). N-acetylneuraminic acid was the sialic acid derivative characterized in the flagellate whole cells. 3. The sialic acid density was estimated as 2.7 x 10(7) residues/cell. 4. The long-chain base dihydrosphingosine, the carbohydrates galactose (67%), glucose (21%) and mannose (12%) as well as the fatty acids myristic (48%) and palmitic (52%) acids were characterized as components of the total glycolipids of T. foetus. 5. Total glycolipids were fractionated: a galactocerebroside and a ganglioside were identified.</p>","PeriodicalId":22539,"journal":{"name":"The International journal of biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0020-711x(88)90360-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14481976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Polyamines and enzymes of polyamines metabolism in the cartilage during embryonic development. 胚胎发育过程中软骨中的多胺和多胺代谢酶。
The International journal of biochemistry Pub Date : 1988-01-01 DOI: 10.1016/0020-711x(88)90357-6
N Bargoni, O Tazartes
{"title":"Polyamines and enzymes of polyamines metabolism in the cartilage during embryonic development.","authors":"N Bargoni,&nbsp;O Tazartes","doi":"10.1016/0020-711x(88)90357-6","DOIUrl":"https://doi.org/10.1016/0020-711x(88)90357-6","url":null,"abstract":"<p><p>1. In chicken embryo cartilage putrescine levels, maximal at day 8, fall by day 16 to a four-fold lower value, which remains unchanged through hatching and in the 12-day-old chick. 2. Spermine and spermidine, initially higher than putrescine, are almost halved between days 8 and 11, and remain constant afterwards. 3. Ornithine decarboxylase is down to 20% of the day 8 value by day 16, and is further reduced in the newly hatched chick. 4. S-Adenosyl-methionine decarboxylase activity shows a 50% reduction between days 8 and 11, and no further changes. 5. Spermidine acetyltransferase activity at day 11 is 30% lower than at day 8, goes back up to the initial level by day 16, and progressively decreases through hatching and the first 12 days of life.</p>","PeriodicalId":22539,"journal":{"name":"The International journal of biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0020-711x(88)90357-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14481973","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 6
Snake venom toxins--II. The primary structures of cytotoxin homologues S3C2 and S4C8 from Aspidelaps scutatus (shield or shield-nose snake) venom. 蛇毒毒素——二。盾鼻蛇毒液中细胞毒素同源物S3C2和S4C8的初级结构。
The International journal of biochemistry Pub Date : 1988-01-01 DOI: 10.1016/0020-711x(88)90361-8
F J Joubert
{"title":"Snake venom toxins--II. The primary structures of cytotoxin homologues S3C2 and S4C8 from Aspidelaps scutatus (shield or shield-nose snake) venom.","authors":"F J Joubert","doi":"10.1016/0020-711x(88)90361-8","DOIUrl":"https://doi.org/10.1016/0020-711x(88)90361-8","url":null,"abstract":"<p><p>1. Cytotoxin homologues S3C2 and S4C8 from Aspidelaps scutatus were purified by gel filtration and ion exchange chromatography. 2. They consist of 63 amino acids including eight half-cystines. The toxicities of S3C2 and S4C8 were determined and LD50 values of 6.6 and 9.4 micrograms/g mouse were, respectively, found. 3. The complete primary structures of toxins S3C2 and S4C8 have been determined. The two toxins resemble the cytotoxin type toxins and in the cytotoxin homologues the ten structurally invariant amino acids of the neurotoxins and the cytotoxins are conserved.</p>","PeriodicalId":22539,"journal":{"name":"The International journal of biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0020-711x(88)90361-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14481977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 4
Effects of fasting and training on pyruvate dehydrogenase activation during exercise. 禁食和训练对运动中丙酮酸脱氢酶激活的影响。
The International journal of biochemistry Pub Date : 1988-01-01 DOI: 10.1016/0020-711x(88)90354-0
J T Brozinick, V K Patel, G L Dohm
{"title":"Effects of fasting and training on pyruvate dehydrogenase activation during exercise.","authors":"J T Brozinick,&nbsp;V K Patel,&nbsp;G L Dohm","doi":"10.1016/0020-711x(88)90354-0","DOIUrl":"https://doi.org/10.1016/0020-711x(88)90354-0","url":null,"abstract":"<p><p>1. The effect of exercise (2 hr treadmill running at 28 m/min) on PDHa (the activity of the active form of pyruvate dehydrogenase) in untrained rats, trained rats (2 hr/d at 25 m/min for 4 wk), and in 24 hr fasted rats was determined. 2. Exercise increased PDHa activity approximately 2 fold in fed-untrained rats. 3. Fasting decreased PDHa activity in sedentary rats to approximately half the activity in fed rats. 4. The increase in PDHa activity during exercise was less in fasted than fed rats. 5. Training did not change the total activity of PDH (phosphorylated plus nonphosphorylated forms) but the percent of PDH in the active form was increased in muscle of trained-rested rats. 6. PDHa activity was unchanged by acute exercise (2.5 hr at 40 m/min) in the trained rats.</p>","PeriodicalId":22539,"journal":{"name":"The International journal of biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0020-711x(88)90354-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14482761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 8
Comparison of adrenergic agonist and insulin effects on 3-O-methyl-D-glucose efflux and sarcolemmal cytochalasin B binding by perfused rat heart. 肾上腺素能激动剂与胰岛素对灌注大鼠心脏3- o -甲基- d -葡萄糖外排及肌层细胞松弛素B结合影响的比较。
The International journal of biochemistry Pub Date : 1988-01-01 DOI: 10.1016/0020-711x(88)90353-9
K C Abel, S Rattigan, M G Clark
{"title":"Comparison of adrenergic agonist and insulin effects on 3-O-methyl-D-glucose efflux and sarcolemmal cytochalasin B binding by perfused rat heart.","authors":"K C Abel,&nbsp;S Rattigan,&nbsp;M G Clark","doi":"10.1016/0020-711x(88)90353-9","DOIUrl":"https://doi.org/10.1016/0020-711x(88)90353-9","url":null,"abstract":"<p><p>1. alpha- and beta-Adrenergic agonists as well as insulin stimulate 3-O-methyl-D-glucose efflux by the perfused rat heart and increase D-glucose inhibitable cytochalasin B binding by isolated sarcolemma. 2. alpha- and beta-Agonists like insulin increase Vmax for 3-O-methyl-D-glucose efflux and increase Bmax for cytochalasin B binding. 3. The effects of alpha- and beta-agonists are totally Ca2+-dependent whilst those of insulin appear to be only partly Ca2+-dependent.</p>","PeriodicalId":22539,"journal":{"name":"The International journal of biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0020-711x(88)90353-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"14030514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 2
Altered Na+-K+-ATPase, cell Na+ and lipid profiles in canine arterial wall with chronic cigarette smoking. 慢性吸烟对犬动脉壁Na+-K+- atp酶、细胞Na+和脂质谱的影响。
The International journal of biochemistry Pub Date : 1988-01-01 DOI: 10.1016/0020-711x(88)90352-7
T N Tulenko, J L Rabinowitz, R H Cox, W P Santamore
{"title":"Altered Na+-K+-ATPase, cell Na+ and lipid profiles in canine arterial wall with chronic cigarette smoking.","authors":"T N Tulenko,&nbsp;J L Rabinowitz,&nbsp;R H Cox,&nbsp;W P Santamore","doi":"10.1016/0020-711x(88)90352-7","DOIUrl":"https://doi.org/10.1016/0020-711x(88)90352-7","url":null,"abstract":"<p><p>1. We evaluated the influence of cigarette smoking on arterial wall membranes, using Na+-K+-ATPase activity, free cholesterol (FC) and phospholipid (PL) contents as indices of membrane structural and functional integrity. 2. Segments of aorta, carotid and femoral arteries were obtained from normal dogs (controls) and dogs subjected to chronic cigarette smoking for 2 yr (12 cigarettes a day). 3. Na+-K+-ATPase activity was assessed in segments of carotid and femoral arteries using a ouabain-sensitive 86Rb uptake procedure for intact tissues. 4. Free cholesterol and phospholipids were separated, identified, and quantitated from extracts of aortic samples by means of two dimensional thin-layer chromatography. 5. Na+-K+-ATPase activity was reduced in the smoker group in both carotid and femoral arteries. This reduced enzyme activity was accompanied by a rise in cell Na+ levels at both arterial sites. 6. Aortic FC was elevated and the PL profile was altered in the smoker group; as a result, phosphatidylcholine was reduced, whereas lysophosphatidylcholine, phosphatidic acid, and cardiolipin were elevated. 7. Phosphatidylethanolamine, phosphatidylinositol, phosphatidylserine and sphingolipid levels were unchanged. In addition, the FC/PL ratio was increased in the smokers. 8. Taken together, the changes in Na+-K+-ATPase activity, FC/PL ratio and phospholipid profiles observed are consistent with the hypothesis that chronic cigarette smoking causes a reorganization of the phospholipid bilayer in the smooth-muscle cell membrane of the arterial wall.</p>","PeriodicalId":22539,"journal":{"name":"The International journal of biochemistry","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"1988-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/0020-711x(88)90352-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"13971073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 13
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