Altered Na+-K+-ATPase, cell Na+ and lipid profiles in canine arterial wall with chronic cigarette smoking.

T N Tulenko, J L Rabinowitz, R H Cox, W P Santamore
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引用次数: 13

Abstract

1. We evaluated the influence of cigarette smoking on arterial wall membranes, using Na+-K+-ATPase activity, free cholesterol (FC) and phospholipid (PL) contents as indices of membrane structural and functional integrity. 2. Segments of aorta, carotid and femoral arteries were obtained from normal dogs (controls) and dogs subjected to chronic cigarette smoking for 2 yr (12 cigarettes a day). 3. Na+-K+-ATPase activity was assessed in segments of carotid and femoral arteries using a ouabain-sensitive 86Rb uptake procedure for intact tissues. 4. Free cholesterol and phospholipids were separated, identified, and quantitated from extracts of aortic samples by means of two dimensional thin-layer chromatography. 5. Na+-K+-ATPase activity was reduced in the smoker group in both carotid and femoral arteries. This reduced enzyme activity was accompanied by a rise in cell Na+ levels at both arterial sites. 6. Aortic FC was elevated and the PL profile was altered in the smoker group; as a result, phosphatidylcholine was reduced, whereas lysophosphatidylcholine, phosphatidic acid, and cardiolipin were elevated. 7. Phosphatidylethanolamine, phosphatidylinositol, phosphatidylserine and sphingolipid levels were unchanged. In addition, the FC/PL ratio was increased in the smokers. 8. Taken together, the changes in Na+-K+-ATPase activity, FC/PL ratio and phospholipid profiles observed are consistent with the hypothesis that chronic cigarette smoking causes a reorganization of the phospholipid bilayer in the smooth-muscle cell membrane of the arterial wall.

慢性吸烟对犬动脉壁Na+-K+- atp酶、细胞Na+和脂质谱的影响。
1. 我们以Na+-K+- atp酶活性、游离胆固醇(FC)和磷脂(PL)含量作为膜结构和功能完整性的指标,评估了吸烟对动脉壁膜的影响。2. 从正常狗(对照)和长期吸烟2年(每天12支)的狗身上获得主动脉、颈动脉和股动脉段。3.Na+-K+- atp酶活性在颈动脉和股动脉段进行评估,使用对完整组织的瓦阿巴因敏感的86Rb摄取程序。4. 采用二维薄层色谱法对主动脉样品提取物中的游离胆固醇和磷脂进行了分离、鉴定和定量分析。5. 吸烟组颈动脉和股动脉Na+-K+- atp酶活性降低。酶活性的降低伴随着两个动脉部位细胞Na+水平的升高。6. 吸烟组主动脉FC升高,PL谱改变;结果,磷脂酰胆碱降低,而溶血磷脂酰胆碱、磷脂酸和心磷脂升高。7. 磷脂酰乙醇胺、磷脂酰肌醇、磷脂酰丝氨酸和鞘脂水平不变。此外,吸烟者的FC/PL比值升高。8. 综上所述,观察到的Na+-K+- atp酶活性、FC/PL比值和磷脂谱的变化与长期吸烟导致动脉壁平滑肌细胞膜磷脂双分子层重组的假设一致。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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