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The Italian journal of biochemistry最新文献

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Curcumin ameliorates oxidative stress during nicotine-induced lung toxicity in Wistar rats. 姜黄素改善尼古丁诱导的Wistar大鼠肺毒性期间的氧化应激。
The Italian journal of biochemistry Pub Date : 2004-07-01
Chandran Kalpana, Venugopal Padmanabhan Menon
{"title":"Curcumin ameliorates oxidative stress during nicotine-induced lung toxicity in Wistar rats.","authors":"Chandran Kalpana,&nbsp;Venugopal Padmanabhan Menon","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Nicotine, a major toxic component of cigarette smoke has been identified as a major risk factor for lung related diseases. In the present study, we evaluated the protective effects of curcumin on lipid peroxidation and antioxidants status in bronchoalveolar lavage fluid (BALF) and bronchoalveolar lavage (BAL) of nicotine treated Wistar rats. Lung toxicity was induced by subcutaneous injection of nicotine at a dose of 2.5 mg/kg body weight (5 days a week, for 22 weeks) and curcumin (80 mg/kg body weight) was given simultaneously by intragastric intubation for 22 weeks. Measurement of biochemical marker enzymes: alkaline phosphatase, lactate dehydrogenase, lipid peroxidation and antioxidants were used to monitor the antiperoxidative effects of curcumin. The increased biochemical marker enzymes as well as lipid peroxides in BALF and BAL of nicotine treated rats was accompanied by a significant decrease in the levels of glutathione, glutathione peroxidase, superoxide dismutase and catalase. Administration of curcumin significantly lowered the biochemical marker enzymes, lipid peroxidation and enhanced the antioxidant status. The results of the present study suggest that curcumin exert its protective effect against nicotine-induced lung toxicity by modulating the biochemical marker enzymes, lipid peroxidation and augmenting antioxidant defense system.</p>","PeriodicalId":22527,"journal":{"name":"The Italian journal of biochemistry","volume":"53 2","pages":"82-6"},"PeriodicalIF":0.0,"publicationDate":"2004-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25069903","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The effect of hydrocarbon addition on the deamidation rate in immune whey proteins. 添加碳氢化合物对免疫乳清蛋白脱酰胺率的影响。
The Italian journal of biochemistry Pub Date : 2004-07-01
Michael Y Bibov, Irina A Sorokina, Alexander I Lukash
{"title":"The effect of hydrocarbon addition on the deamidation rate in immune whey proteins.","authors":"Michael Y Bibov,&nbsp;Irina A Sorokina,&nbsp;Alexander I Lukash","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The influence of some hydrocarbons that are often used at different stages of immunobiological preparation's production as stabilizers of biological activity on the dynamics of nonenzymatic deamidation in proteins of immune whey against conditionally pathogenic microorganisms obtained by means of membrane ultrafiltration technology is investigated. Preparations of whey were incubated in 10 per cent solutions of glucose, fructose and sorbitol at the conditions similar to physiological ones (0.9% NaCl, pH 5.5) and temperature of about +4 degrees C and +35 degrees C for 7, 14 and 28 days. A sample dissolved in 0.9% NaCl (pH 5.5) without addition of hydrocarbons was used as a \"control preparate\". All explored substances brought about the suppressive effect on deamidation rate of asparaginyl residues whereas that of glutaminyl residues, on the contrary, was obviously increased. The possible reasons for these observations are discussed.</p>","PeriodicalId":22527,"journal":{"name":"The Italian journal of biochemistry","volume":"53 2","pages":"87-91"},"PeriodicalIF":0.0,"publicationDate":"2004-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25069904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Expression and characterization of human tumor necrosis factor-related apoptosis inducing ligand in methylotrophic yeast Pichia pastoris. 人肿瘤坏死因子相关凋亡诱导配体在甲基营养酵母毕赤酵母中的表达与表征。
The Italian journal of biochemistry Pub Date : 2004-07-01
Jinzhi Wang, Juxing Chen, Mian Wu
{"title":"Expression and characterization of human tumor necrosis factor-related apoptosis inducing ligand in methylotrophic yeast Pichia pastoris.","authors":"Jinzhi Wang,&nbsp;Juxing Chen,&nbsp;Mian Wu","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Recombinant human TRAIL was successfully expressed in a secreted form in methyltrophic yeast Pichia pastoris induced by methanol. The expressive product was immunoreactive to TRAIL antibody. The addition of the expressive product into cultured human lung cancer cell A549 showed moderate cell death, typical morphological characterization of apoptotic bodies, and DNA fragmentation. This result provided a new method to produce recombinant human TRAIL as a cancer therapeutic drug.</p>","PeriodicalId":22527,"journal":{"name":"The Italian journal of biochemistry","volume":"53 2","pages":"67-72"},"PeriodicalIF":0.0,"publicationDate":"2004-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25069901","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Increased serum levels of interleukin-18 in patients with diabetic nephropathy. 糖尿病肾病患者血清白细胞介素-18水平升高。
The Italian journal of biochemistry Pub Date : 2004-07-01
Randa Abdel Kader Mahmoud, Sahar Ahmed Abou el-Ezz, Adalat Said Hegazy
{"title":"Increased serum levels of interleukin-18 in patients with diabetic nephropathy.","authors":"Randa Abdel Kader Mahmoud,&nbsp;Sahar Ahmed Abou el-Ezz,&nbsp;Adalat Said Hegazy","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In the present study we measured interleukin-18 (IL-18) and tumour necrosis factor-alpha (TNF-alpha) levels by enzyme linked immunosorbent assay (ELISA) in sera from 65 diabetic [30 with type 1 insulin dependent diabetes mellitus (IDDM) and 35 with type 2 non-insulin dependent diabetes mellitus (NIDDM)] patients and 15 healthy volunteers, to investigate their associations with metabolic parameters and to elucidate their roles in the pathogenesis of diabetic complications especially diabetic nephropathy. Levels of IL-18 and TNF-alpha were significantly higher in both IDDM and NIDDM individuals as compared to the control group. Similarly, their levels in patients with diabetic nephropathy increased gradually according to the clinical stage of the disease, being highest in macroalbuminuric stage. Correlation analyses showed that the serum IL-18 and TNF-alpha concentration were positively correlated with each other and positively with fasting plasma glucose (FPG), 2h postprandial glucose, glycosylated hemoglobin (HbA1c), triglyceride, and urinary albumin levels and negative correlation between TNF-alpha and high density lipoprotein cholesterol (HDL-C) were also found in diabetic subjects. High serum levels of IL-18 and TNF-alpha suggested that they might play a role in the pathogenesis of DM and in the development of nephropathy in diabetic patients whether of type 1 or 2.</p>","PeriodicalId":22527,"journal":{"name":"The Italian journal of biochemistry","volume":"53 2","pages":"73-81"},"PeriodicalIF":0.0,"publicationDate":"2004-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25069902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
GPI-anchored proteins and lipid rafts. gpi锚定蛋白和脂筏。
The Italian journal of biochemistry Pub Date : 2004-07-01
Vanessa Sangiorgio, Marina Pitto, Paola Palestini, Massimo Masserini
{"title":"GPI-anchored proteins and lipid rafts.","authors":"Vanessa Sangiorgio,&nbsp;Marina Pitto,&nbsp;Paola Palestini,&nbsp;Massimo Masserini","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Several proteins are anchored to membranes via a post-translational lipid modification, the glycosylphosphatidylinositol (GPI) anchor. In mammals and other vertebrates, GPI-anchored proteins have been found in almost all tissues and cells examined. Several studies have provided significant insight into the functions of this ubiquitous modification. An intriguing relevant feature of GPI-anchored proteins is their association with lipid rafts, specialized regions of elevated cholesterol and sphingolipid content, that are present within most cell membranes. In addition to the structure and biosynthesis of the GPI-anchor, recent researches have focused on its molecular interaction with lipid rafts and the biological meaning of such interaction. The aim of this review is to examine the emerging evidences of association between lipid rafts and GPI-anchored proteins, and their relationship with the modulation of important cellular functions such as protein/lipid sorting, signaling mechanisms and with human disease.</p>","PeriodicalId":22527,"journal":{"name":"The Italian journal of biochemistry","volume":"53 2","pages":"98-111"},"PeriodicalIF":0.0,"publicationDate":"2004-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25069906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Ionic regulation of proteins. 蛋白质的离子调控。
The Italian journal of biochemistry Pub Date : 2004-07-01
Enrico Di Stasio
{"title":"Ionic regulation of proteins.","authors":"Enrico Di Stasio","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Ion binding modulates the structural and the functional properties of several proteins. The molecular bases of such interactions depend on the charge density of ions, in turn influencing their ability to bind water molecules. The broad range of proteins submitted to ionic control, makes it interesting a general evaluation of the role played by ions in the homeostasis of intra and extracellular compartments and in a number of physio-pathological conditions, with special attention to the coagulation cascade and cryoglobulin aggregation.</p>","PeriodicalId":22527,"journal":{"name":"The Italian journal of biochemistry","volume":"53 2","pages":"112-9"},"PeriodicalIF":0.0,"publicationDate":"2004-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25070385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Structure and function of the plant toxin ricin, an N-glycosidase enzyme. 植物毒素蓖麻毒素n -糖苷酶的结构和功能。
The Italian journal of biochemistry Pub Date : 2004-07-01
Rodolfo Ippoliti
{"title":"Structure and function of the plant toxin ricin, an N-glycosidase enzyme.","authors":"Rodolfo Ippoliti","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":22527,"journal":{"name":"The Italian journal of biochemistry","volume":"53 2","pages":"92-7"},"PeriodicalIF":0.0,"publicationDate":"2004-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25069905","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Curcumin influences hepatic expression patterns of matrix metalloproteinases in liver toxicity. 姜黄素影响肝毒性中基质金属蛋白酶的肝脏表达模式。
The Italian journal of biochemistry Pub Date : 2004-07-01
Rajagopalan Rukkumani, Kode Aruna, Penumathsa Suresh Varma, Venugopal Padmanabhan Menon
{"title":"Curcumin influences hepatic expression patterns of matrix metalloproteinases in liver toxicity.","authors":"Rajagopalan Rukkumani,&nbsp;Kode Aruna,&nbsp;Penumathsa Suresh Varma,&nbsp;Venugopal Padmanabhan Menon","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Hepatic fibrosis is a result of an imbalance between enhanced matrix synthesis and diminished breakdown of connective tissue proteins, the net result of which is increased deposition of Extra Cellular Matrix. In this concept Matrix Metalloproteinases play an important role because their activity is largely responsible for extra cellular matrix breakdown. In the present study we have tested the influence of curcumin, the active principle of turmeric, on matrix metalloproteinase expression during alcohol and thermally oxidised sunflower oil induced liver toxicity. Male albino Wistar rats were used for the study. The matrix metalloproteinase expressions were found to be increased significantly in alcohol as well as thermally oxidised sunflower oil groups and on treatment with curcumin there was a significant decrease. In alcohol + thermally oxidised sunflower oil group, we found a significant decrease in matrix metalloproteinase activities. Administration of curcumin significantly improved their activities. From the results obtained, we could conclude that curcumin influences the hepatic matrix metalloproteinases and effectively protects liver against alcohol and delta PUFA induced toxicity.</p>","PeriodicalId":22527,"journal":{"name":"The Italian journal of biochemistry","volume":"53 2","pages":"61-6"},"PeriodicalIF":0.0,"publicationDate":"2004-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25069900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
4-hydroxy-2,3 trans-nonenal nuclear concentration inversely correlates with the growth rate of T leukemia cells. 4-羟基-2,3反式壬烯醛核浓度与T白血病细胞生长速率呈负相关。
The Italian journal of biochemistry Pub Date : 2004-03-01
Natalia Calonghi, Concettina Cappadone, Eleonora Pagnotta, Carla Boga, Carlo Bertucci, Jessica Fiori, Pietro Traldi, Lanfranco Masotti
{"title":"4-hydroxy-2,3 trans-nonenal nuclear concentration inversely correlates with the growth rate of T leukemia cells.","authors":"Natalia Calonghi,&nbsp;Concettina Cappadone,&nbsp;Eleonora Pagnotta,&nbsp;Carla Boga,&nbsp;Carlo Bertucci,&nbsp;Jessica Fiori,&nbsp;Pietro Traldi,&nbsp;Lanfranco Masotti","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>4-hydroxy-2,3 trans-nonenal is the major diffusible product generated by linoleic and arachidonic acids peroxidation. Its endogenous content is inversely related to the rate of cell proliferation and directly related to the level of cell differentiation. As previously reported, the nuclear localization of the aldehyde has been observed by means of a fluorescent antibody and confocal microscopy, and its concentration measured by electrospray/mass spectrometry, a sensitive and selective method for 4-hydroxy-2,3 trans-nonenal determination, on nuclear extracts of leukemic cells. With the aim to establish a possible correlation between the peroxidation product nuclear concentration and cell growth rate, Jurkat 6 leukemic cells have been used and the aldehyde measured by electrospray/mass spectrometry. The cells arrested in G1 show a content of 4-hydroxy-2,3 trans-nonenal significantly increased with respect to control ones.</p>","PeriodicalId":22527,"journal":{"name":"The Italian journal of biochemistry","volume":"53 1","pages":"28-33"},"PeriodicalIF":0.0,"publicationDate":"2004-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24672869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Genesis and evolution of the first biochemistry. 第一个生物化学的发生与演化。
The Italian journal of biochemistry Pub Date : 2004-03-01
Francesco Ranaldi, Raimonda Ottaviani, Duccio Vanni, Elizabeth Guerin, Eugenio Giachetti, Paolo Vanni
{"title":"Genesis and evolution of the first biochemistry.","authors":"Francesco Ranaldi,&nbsp;Raimonda Ottaviani,&nbsp;Duccio Vanni,&nbsp;Elizabeth Guerin,&nbsp;Eugenio Giachetti,&nbsp;Paolo Vanni","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":22527,"journal":{"name":"The Italian journal of biochemistry","volume":"53 1","pages":"1-7"},"PeriodicalIF":0.0,"publicationDate":"2004-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24673524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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