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The Italian journal of biochemistry最新文献

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Suicidal dephosphorylation of thiamine pyrophosphate coupled with pyruvate dehydrogenase complex. 与丙酮酸脱氢酶复合物偶联的自杀性去磷酸化硫胺素焦磷酸。
The Italian journal of biochemistry Pub Date : 2004-12-01
Slawomir Strumilo, Pawel Dobrzyn, Jan Czerniecki, Adam Tylicki
{"title":"Suicidal dephosphorylation of thiamine pyrophosphate coupled with pyruvate dehydrogenase complex.","authors":"Slawomir Strumilo,&nbsp;Pawel Dobrzyn,&nbsp;Jan Czerniecki,&nbsp;Adam Tylicki","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Earlier it was noted that purified pyruvate dehydrogenase complex (PDC) produced by \"Sigma\" usually contains almost saturating amounts of thiamine pyrophosphate (ThPP). In this communication we present the observation that the endogenous ThPP coupled to PDC is dephosphorylated while staying at -10 degrees C, because in the enzyme preparation thiamine monophosphate and un-phosphorylated thiamine appear (HPLC determination). Under the same conditions exogenous ThPP is not dephosphorylated despite contact with the PDC preparation. This may suggest that interactions of some active groups of the enzyme with molecules of endogenous ThPP leads to break-up of the phosphoesters bonds, and destruction of the coenzyme. Decrease of PDC activity during storage is not in proportion with the degree of ThPP dephosphorylation. However the observed instability of PDC activity may be a consequence of the spontaneous process of its coenzyme autodestruction.</p>","PeriodicalId":22527,"journal":{"name":"The Italian journal of biochemistry","volume":"53 4","pages":"131-4"},"PeriodicalIF":0.0,"publicationDate":"2004-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24881863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Adenosine: old passion, new perspectives. 腺苷:旧的激情，新的视角。
The Italian journal of biochemistry Pub Date : 2004-12-01
Lucacchini Antonio, M Letizia Trincavelli
{"title":"Adenosine: old passion, new perspectives.","authors":"Lucacchini Antonio,&nbsp;M Letizia Trincavelli","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In this review is summarized my research in the adenosine field from the beginning of my carrier to day. My research began preparing the degree thesis with Prof. Carlo Alfonso Rossi, about the purification and characterization of adenosine deaminase. My scientific interest for adenosine has spread during the years and I have been interested in the study of ARs and their related transduction and in the study of molecular mechanisms involved in homologous and heterologous AR receptor regulation.</p>","PeriodicalId":22527,"journal":{"name":"The Italian journal of biochemistry","volume":"53 4","pages":"141-7"},"PeriodicalIF":0.0,"publicationDate":"2004-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24881867","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Evaluation of the lipid composition of human healthy and neoplastic renal tissues. 人类健康和肿瘤肾组织脂质组成的评价。
The Italian journal of biochemistry Pub Date : 2004-12-01
Vitaliano Tugnoli, Andrea Trinchero, Maria Raffaella Tosi
{"title":"Evaluation of the lipid composition of human healthy and neoplastic renal tissues.","authors":"Vitaliano Tugnoli,&nbsp;Andrea Trinchero,&nbsp;Maria Raffaella Tosi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The assumption that kidney tumors are characterized by high lipid content has directed our research towards the evaluation and comparison of lipids in healthy and neoplastic human renal tissues. This research appears to have marked importance owing to the role of lipids in cancer cell biochemistry. Nuclear Magnetic Resonance spectroscopy and chromatographic methods allow the full description of the lipidic profile typical of intact medulla, intact cortex, clear-cell renal carcinomas, chromophobe-cell renal carcinomas, oncocytomas and two samples of medulla infiltrated by a nephrocarcinoma. Significant differences among healthy, benign and malignant tissues were shown, and the results were discussed in relation to the different roles of each lipidic component and compared with the literature data.</p>","PeriodicalId":22527,"journal":{"name":"The Italian journal of biochemistry","volume":"53 4","pages":"169-82"},"PeriodicalIF":0.0,"publicationDate":"2004-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24881872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Should we teach homeopathy to our medical students? 我们应该教医学生顺势疗法吗?
The Italian journal of biochemistry Pub Date : 2004-12-01
Andrea Bellelli
{"title":"Should we teach homeopathy to our medical students?","authors":"Andrea Bellelli","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":22527,"journal":{"name":"The Italian journal of biochemistry","volume":"53 4","pages":"125-9"},"PeriodicalIF":0.0,"publicationDate":"2004-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24881860","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Guanidine-induced dissociation of mitochondrial F1-ATPase. 胍诱导线粒体 F1-ATP 酶解离。
The Italian journal of biochemistry Pub Date : 2004-12-01
Stavros Papageorgiou, Giancarlo Solaini
{"title":"Guanidine-induced dissociation of mitochondrial F1-ATPase.","authors":"Stavros Papageorgiou, Giancarlo Solaini","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The effect of guanidine hydrochloride on ATPase activity, gel filtration, turbidity, and the fluorescence emission intensity of mitochondrial F1-ATPase was examined. Purified F1 from bovine heart mitochondria was slowly inactivated at low denaturant concentration, and inactivation was associated with delta and epsilon subunit dissociation. delta and epsilon subunits were bound together to form a stable and soluble heterodimer. In parallel, appearance of turbidity was observed. This was caused by the formation of alpha3beta3gamma non-covalent aggregates, as analyzed by SDS-PAGE. Short periods of exposition of the F1 complex to high concentrations of guanidine hydrochloride (0.8-3 M) again induced deltaepsilon dissociation as a heterodimer and the formation of an inactive alpha3beta3gamma subcomplex. This eventually dissociated progressively into single subunits caused by partial unfolding, as evidenced through changes of the protein intrinsic fluorescence emission. Our results suggest that the delta and epsilon subunits are loosely bound to alpha3beta3gamma , and play an important role in determining structural stability to isolated mitochondrial F1-ATPase.</p>","PeriodicalId":22527,"journal":{"name":"The Italian journal of biochemistry","volume":"53 4","pages":"148-56"},"PeriodicalIF":0.0,"publicationDate":"2004-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24881869","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Nutritional interventions to counteract oxidative stress in cardiac cells. 营养干预对抗心脏细胞氧化应激。
The Italian journal of biochemistry Pub Date : 2004-12-01
Silvana Hrelia, Alessandra Bordoni, Cristina Angeloni, Emanuela Leoncini, PierLuigi Biagi
{"title":"Nutritional interventions to counteract oxidative stress in cardiac cells.","authors":"Silvana Hrelia,&nbsp;Alessandra Bordoni,&nbsp;Cristina Angeloni,&nbsp;Emanuela Leoncini,&nbsp;PierLuigi Biagi","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Preventing oxidative damage in the heart is subject of considerable investigation and studies developing nutritional intervention methods to attenuate or prevent the resulting pathological state of free radical damage are now emerging. In this light, a dietary intervention directed to increase the daily intake of antioxidant molecules represents a fundamental step to achieve a beneficial result. In this minireview the attention is focused on the damage induced in cultured cardiac cells by the antitumoral doxorubicin, known for its cardiotoxicity, and by hypoxia/reoxygenation that occur in a wide variety of important clinical conditions. The identification of antioxidant molecules having specific effectiveness in a particular cell type may be useful for the development of a prevention strategy specific for free radical induced-diseases related to that cell type. Although the connection between consumption of foods rich in polyphenolic compounds and the decreased risk of cardiovascular disease has been reported, the role of different antioxidant molecules contained in foods is still to be elucidated. The protective effect of the polyphenolic components of green tea in the prevention/counteraction of cell damage induced in the heart by doxorubicin or hypoxia/reoxygenation has been discussed.</p>","PeriodicalId":22527,"journal":{"name":"The Italian journal of biochemistry","volume":"53 4","pages":"157-63"},"PeriodicalIF":0.0,"publicationDate":"2004-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24881870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Special issue in honour of the memory of professor Carlo Alfonso Rossi. 纪念卡洛·阿方索·罗西教授的特刊。
The Italian journal of biochemistry Pub Date : 2004-12-01
{"title":"Special issue in honour of the memory of professor Carlo Alfonso Rossi.","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":22527,"journal":{"name":"The Italian journal of biochemistry","volume":"53 4","pages":"130-83"},"PeriodicalIF":0.0,"publicationDate":"2004-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25280296","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Twenty years of hunting for proteins with Carlo Alfonso Rossi. 和卡洛·阿方索·罗西一起寻找蛋白质20年。
The Italian journal of biochemistry Pub Date : 2004-12-01
Fiorenzo Stirpe
{"title":"Twenty years of hunting for proteins with Carlo Alfonso Rossi.","authors":"Fiorenzo Stirpe","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The research accomplished in more thanr twenty years of collaboration with Carlo Alfonso Rossi is reviewed. Several lectins and toxic and non-toxic ribosome-inactivating proteins were identified, purified and characterized. The general properties of the proteins are described.</p>","PeriodicalId":22527,"journal":{"name":"The Italian journal of biochemistry","volume":"53 4","pages":"164-8"},"PeriodicalIF":0.0,"publicationDate":"2004-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24881871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Low molecular weight protein tyrosine phosphatase: catalytic mechanism and physiologic function. 低分子量蛋白酪氨酸磷酸酶的催化机制和生理功能。
The Italian journal of biochemistry Pub Date : 2004-12-01
Giampietro Ramponi
{"title":"Low molecular weight protein tyrosine phosphatase: catalytic mechanism and physiologic function.","authors":"Giampietro Ramponi","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":22527,"journal":{"name":"The Italian journal of biochemistry","volume":"53 4","pages":"183"},"PeriodicalIF":0.0,"publicationDate":"2004-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"24881873","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Abstracts of the 49th Meeting of the Italian Society for Biochemistry and Molecular Biology. Riccione, Italy, September 28-October 1, 2004. 意大利生物化学和分子生物学学会第49届会议摘要。里奇奥内，意大利，2004年9月28日至10月1日。
The Italian journal of biochemistry Pub Date : 2004-09-01
{"title":"Abstracts of the 49th Meeting of the Italian Society for Biochemistry and Molecular Biology. Riccione, Italy, September 28-October 1, 2004.","authors":"","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":22527,"journal":{"name":"The Italian journal of biochemistry","volume":"53 3","pages":"1-363"},"PeriodicalIF":0.0,"publicationDate":"2004-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25106565","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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