The Indonesian Biomedical Journal最新文献_第8页

Dioscorea alata L. Tubers Improve Diabetes through Anti-hyperglycemia, Anti-inflammation, Ameliorate Insulin Resistance and Mitochondrial Dysfunction 薯蓣块茎通过抗高血糖、抗炎症、改善胰岛素抵抗和线粒体功能障碍改善糖尿病

The Indonesian Biomedical Journal Pub Date : 2022-12-01 DOI: 10.18585/inabj.v14i4.1966

Sri Nabawiyati Nurul Makiyah, M. Kita, I. Setyawati, Sri Tasminatun

{"title":"Dioscorea alata L. Tubers Improve Diabetes through Anti-hyperglycemia, Anti-inflammation, Ameliorate Insulin Resistance and Mitochondrial Dysfunction","authors":"Sri Nabawiyati Nurul Makiyah, M. Kita, I. Setyawati, Sri Tasminatun","doi":"10.18585/inabj.v14i4.1966","DOIUrl":"https://doi.org/10.18585/inabj.v14i4.1966","url":null,"abstract":"BACKGROUND: Dioscorea alata L. tubers (DA) are suspected to prevent diabetes mellitus (DM) because they have a low glycemic index. However, only a few reports about the anti-diabetic effect of DA were reported up to date. This study aims to analyze the effect of DA consumption on several diabetic biomarkers through in vitro, in vivo, and in silico analysis.METHODS: In vitro experiments were conducted by observing the anti-inflammatory activity of DA extract, steroidal saponins (SDA) isolated from DA, and diosgenin in lymphocyte cell cultures. The tumor necrosis factor (TNF)-α and interferon (IFN)-γ percentages were analyzed by flow cytometry. In vivo study involved 24 healthy adolescents that were given a boiled DA 10 hours post-prandial. The blood sugar levels were measured at 0, 30, 60, and 120 min after treatment. Furthermore, the in silico study was carried out by analyzing the active compounds and predicting the biological activity, the target proteins, and interactions of target proteins with compounds contained in DA .RESULTS: DA extract, SDA isolated from DA, and diosgenin at 50 µg/mL significantly reduced pro-inflammatory cytokines TNF-α and IFN-γ in lymphocyte cell culture. The blood glucose levels in the DA group were lower at 30 and 60 min after treatment. Based on the in silico study, the anti-diabetic activity of DA was speculated to be attributed to the mechanisms of anti-hyperglycemia, prevention of mitochondrial dysfunction, anti-inflammation, and treated insulin resistance. Several proteins included in the DM pathway became the protein target of compounds contained in DA.CONCLUSION: DA potentially have an anti-diabetic activity through several mechanisms.KEYWORDS: hyperglycemia, inflammation, insulin resistance, yam","PeriodicalId":22516,"journal":{"name":"The Indonesian Biomedical Journal","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82464357","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of Estrogen Receptor Alpha rs3798577 Polymorphism in Breast Carcinoma Risk Determination 雌激素受体α rs3798577多态性在乳腺癌风险测定中的作用

The Indonesian Biomedical Journal Pub Date : 2022-12-01 DOI: 10.18585/inabj.v14i4.2002

P. Kumaladewi, W. Harahap, Bastian Nova, I. Widodo, Ramadhan Karsono, F. Sandra, B. Hernowo

{"title":"Role of Estrogen Receptor Alpha rs3798577 Polymorphism in Breast Carcinoma Risk Determination","authors":"P. Kumaladewi, W. Harahap, Bastian Nova, I. Widodo, Ramadhan Karsono, F. Sandra, B. Hernowo","doi":"10.18585/inabj.v14i4.2002","DOIUrl":"https://doi.org/10.18585/inabj.v14i4.2002","url":null,"abstract":"BACKGROUND: Interaction between estrogen and estrogen receptor (ER) takes part in the regulation and differentiation of breast tumorigenesis. Some ERα polymorphisms, including ERα rs3798577, are reported to be associated with the risk and aggressiveness of breast carcinoma since the site was reported to be targeted by microRNA, which can further modulate the ERα expression. Hence, this study was conducted to disclose the possible role of ERα SNP rs3798577 on breast carcinoma patients.METHODS: Samples were taken from the post-mastectomy breast carcinoma tissues of female patients and screened based on the completeness of medical and histopathological records. DNA isolation was proceeded using real time-polymerase chain reaction (RT-PCR) then analyzed for high resolution melting (HRM). The nucleotide base sequence was then analyzed based on rs3798577 ERα polymorphism. ER immunohistochemistry test was carried out and counted quantitatively based on the staining intensity and the percentage of the stained cells.RESULTS: Out of 65 samples, there were 33 samples as wild type and 32 samples as variant type. Most variant and wild type had >80% ERα percentage. Most variant type had middle ERα intensity, while wild type had strong ERα intensity. Higher percentage of variant type (52.2%) was found with weak ERα histoscore, meanwhile higher percentage of wild type (52.4%) was found with strong ERα histoscore, but not significant (p=0.725).CONCLUSION: ERα rs3798577 variant type had a lower ERα intensity and weaker ERα histoscore compared to the wild type, suggesting that ERα rs3798577 polymorphism might play a role in breast carcinoma risk determination.KEYWORDS: breast cancer, ERα, rs3798577, polymorphism, immunoexpression","PeriodicalId":22516,"journal":{"name":"The Indonesian Biomedical Journal","volume":"54 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87073045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Probiotic Lactobacillus acidophilus FNCC 0051 Improves Pancreatic Histopathology in Streptozotocin-induced Type-1 Diabetes Mellitus Rats 益生菌嗜酸乳杆菌FNCC 0051改善链脲佐菌素诱导的1型糖尿病大鼠胰腺组织病理学

The Indonesian Biomedical Journal Pub Date : 2022-12-01 DOI: 10.18585/inabj.v14i4.2047

M. Sariyanti, Tiara Ayoe Andita, Noor Diah Erlinawati, Elvira Yunita, A. A. Nasution, Kartika Sari, Nikki Aldi Massardi, Sylvia Rianissa Putri

{"title":"Probiotic Lactobacillus acidophilus FNCC 0051 Improves Pancreatic Histopathology in Streptozotocin-induced Type-1 Diabetes Mellitus Rats","authors":"M. Sariyanti, Tiara Ayoe Andita, Noor Diah Erlinawati, Elvira Yunita, A. A. Nasution, Kartika Sari, Nikki Aldi Massardi, Sylvia Rianissa Putri","doi":"10.18585/inabj.v14i4.2047","DOIUrl":"https://doi.org/10.18585/inabj.v14i4.2047","url":null,"abstract":"BACKGROUND: Intestinal microbial dysbiosis and its metabolites can affect the immune activity of intestinal mucosal cells, causing insulitis and pancreatic β-cell death. Probiotic Lactobacillus acidophilus plays an important role in reducing inflammatory cytokines, hence improves oxidative stress that affects pancreatic β-cell apoptosis. Current study examined the feature of pancreatic histopathology affected by the administration of probiotic L. acidophilus in rats with type-1 diabetes mellitus (DM) induced by streptozotocin (STZ).METHODS: Twelve rats were induced by STZ at double dose of 50 mg/kgBB before administered with probiotic L. acidophilus at a dose of 1.5x10 8 or 1.5x10 9 CFU/mL/day, while other 4 rats were used as control. After 21 days of the L. acidophilus treatment, the average of fasting blood glucose (FBG) levels of rats were measured, then the pancreatic histopathology was assessed to evaluate the degree of insulitis in islet of Langerhans.RESULTS: The induction of STZ had been succeeded to increase blood glucose levels, which indicate DM condition. The highest FBG level after 21 days of treatment was found in DM group with glucose level of 512±81.51 mg/dL. The administration of probiotic L. acidophilus during 21 days treatment at both dose 1.5x10 8 and 1.5x10 9 CFU/mL/day significantly improved pancreatic histopathology (p=0.04 and p=0.034, respectively), with significant decrease on insulitis scores compared to DM group.CONCLUSION: The administration of L. acidophilus at both dose of 1.5x10 8 and 1.5x10 9 CFU/mL/day for 21 days can improve pancreatic histopathology of type-1 DM rats induced by STZ, therefore probiotic L. acidophilus may be potential as supplementation treatment for type-1 DM.KEYWORDS: Lactobacillus acidophilus, pancreatic histopathology, streptozotocin, type-1 diabetes mellitus","PeriodicalId":22516,"journal":{"name":"The Indonesian Biomedical Journal","volume":"48 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79754734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Caffeic Acid Inhibits Tumour Mass Formation in MG-63 Cells-induced Nude Mice 咖啡酸抑制MG-63细胞诱导的裸鼠肿瘤团块形成

The Indonesian Biomedical Journal Pub Date : 2022-12-01 DOI: 10.18585/inabj.v14i4.2078

F. Sandra, Dewi Ranggaini, Laifa Annisa Hendamin, Nurrani Mustika Dewi, M. Djamil

{"title":"Caffeic Acid Inhibits Tumour Mass Formation in MG-63 Cells-induced Nude Mice","authors":"F. Sandra, Dewi Ranggaini, Laifa Annisa Hendamin, Nurrani Mustika Dewi, M. Djamil","doi":"10.18585/inabj.v14i4.2078","DOIUrl":"https://doi.org/10.18585/inabj.v14i4.2078","url":null,"abstract":"BACKGROUND: Formation of tumour mass is one symptom of osteosarcoma development. Caffeic acid has been known to provide effective treatment but has less side effect for some cancer therapy. Studies reported that caffeic acid might promote apoptosis in MG-63 osteosarcoma cells, however, the effect of caffeic acid treatment in preventing tumour mass formation has not been well elucidated, especially in MG-63 cells-induced nude mice in vivo.METHODS: MG-63 cells were pre-treated with 0, 1, or 10 µg/mL caffeic acid, and 6 hours after pre-treatment, MG-63 cells were injected into subcutaneous space of mice to induce osteosarcoma. Another model was also created by subcutaneously injecting MG-63 cells to the back of mice, and after 48 days, the visible tumour mass was injected intra-tumour with 0 or 10 µg/mL caffeic acid every 7 days for 6 times. After 90 days, mice were anaesthetised, and the nodule pictures were taken for observation and measurement. RESULTS: In pre-treated MG-63 cells-induced mice, volumes of the mass decreased in reverse with the dose of caffeic acid given. Ten µg/mL caffeic acid pre-treatment was able to significantly lower the mass volume compared to the untreated (p<0.05). Meanwhile, the intra-tumour treatment of 10 µg/mL caffeic acid, even though not significant, was able to inhibit tumour mass formation.CONCLUSION: Results of caffeic acid pre-treatment and caffeic acid treatment in tumour mass of mice show that caffeic acid is able to inhibit the MG-63 cells formation. This suggests that caffeic acid can be a potential anti-cancer agent.KEYWORDS: caffeic acid, osteosarcoma, MG-63 cells, tumour mass","PeriodicalId":22516,"journal":{"name":"The Indonesian Biomedical Journal","volume":"122 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86571675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

OXTR Gene mRNA Expression is Correlated to Prosocial Behavior of Children in the Golden Generation Program of Nusa Tenggara Barat OXTR基因mRNA表达与努沙登加拉高原黄金一代计划儿童亲社会行为相关

The Indonesian Biomedical Journal Pub Date : 2022-12-01 DOI: 10.18585/inabj.v14i4.2021

Wilya Isnaeni, S. As'ad, M. Hatta, S. Syamsuddin, F. R. Andiwijaya, H. Kadriyan

{"title":"OXTR Gene mRNA Expression is Correlated to Prosocial Behavior of Children in the Golden Generation Program of Nusa Tenggara Barat","authors":"Wilya Isnaeni, S. As'ad, M. Hatta, S. Syamsuddin, F. R. Andiwijaya, H. Kadriyan","doi":"10.18585/inabj.v14i4.2021","DOIUrl":"https://doi.org/10.18585/inabj.v14i4.2021","url":null,"abstract":"BACKGROUND: Cases of undernutrition, such as stunting and wasting, in Nusa Tenggara Barat (NTB), Indonesia, was found to be relatively high. Therefore, local government launched a golden generation program called GEN NTB to improve the quality of human resources by achieving a healthy, intelligent, devout, and productive generation in 2045. One of the genes known to be related with prosocial behavior is the oxytocin transferase (OXTR) gene. This study was conducted to determine the association between OXTR gene mRNA expression and prosocial behavior of the GEN NTB children.METHODS: This was an analytical observational case-control study involving 25 children as GEN NTB samples and 26 children as controls. Blood samples were tested for OXTR protein level with enzyme-linked immunosorbent assay (ELISA), and OXTR mRNA expression with real time polymerase chain reaction (RT-PCR). Prosocial behavior was characterized and determined by using a rating method, which valued from 1 to 4 for poor to very good behavior.RESULTS: The average OXTR protein levels of the GEN NTB group was 88.28 ng/mL, which were higher than the average OXTR protein levels of control group (2.41 ng/mL). According to fold change analysis, the OXTR mRNA expression in GEN NTB group was also higher than the control group (10.91 vs. 6.40). Interestingly, observations on the prosocial behavior of the GEN NTB group showed significantly higher rate values compared to the control group (17.3 vs. 8.0, p=0.034). Hence, these findings showed that the OXTR protein level and OXTR mRNA expression was correlated with the better prosocial behavior.CONCLUSION: Higher rating of prosocial behavior of the GEN NTB children is related to the higher OXTR mRNA expression levels. This might be attributed to the interventions of GEN NTB program that may elevate children's quality of life since early childhood.KEYWORDS: GEN NTB, OXTR protein, mRNA expression, prosocial behavior, children","PeriodicalId":22516,"journal":{"name":"The Indonesian Biomedical Journal","volume":"28 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"81910570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

3D Biosilica Scaffolds from Melophlus sarasinorum and Xestospongia testudinaria Indonesian Sponges are Biocompatible for Cell Growth and Differentiation of Human Wharton’s Jelly Mesenchymal Stem Cell in Bone Tissue Engineering 三维生物硅支架在骨组织工程中对人华顿水母间充质干细胞的生长和分化具有生物相容性

The Indonesian Biomedical Journal Pub Date : 2022-12-01 DOI: 10.18585/inabj.v14i4.1895

Soraya Rahmanisa, E. Prajatelistia, I. Wibowo, A. Barlian

{"title":"3D Biosilica Scaffolds from Melophlus sarasinorum and Xestospongia testudinaria Indonesian Sponges are Biocompatible for Cell Growth and Differentiation of Human Wharton’s Jelly Mesenchymal Stem Cell in Bone Tissue Engineering","authors":"Soraya Rahmanisa, E. Prajatelistia, I. Wibowo, A. Barlian","doi":"10.18585/inabj.v14i4.1895","DOIUrl":"https://doi.org/10.18585/inabj.v14i4.1895","url":null,"abstract":"BACKGROUND: Biosilica derived from Indonesian marine sponge Melophlus sarasinorum and Xestospongia testudinaria is one of the biomaterials that can be developed together with synthetic polymer as a composite. Poly E-caprolactone (PCL) used as a composite role as an osteoconductive material together with biosilica and also tailored the slow rate of degradation in the body. This study aimed to create a biocompatible biosilica-based scaffold and supports osteogenic differentiation of human Wharton's Jelly mesenchymal stem cell (hWJ-MSCs).METHODS: Biosilica was extracted from M. sarasinorum and X. testudinaria with the acid digestion method. Scaffold was prepared using the salt leaching method. The composite scaffolds were made from seven different biosilica extract and PCL. All of the scaffolds were tested for the cell morphology, Fourier-transform infrared spectroscopy (FTIR), immunocytochemistry, and cytotoxicity.RESULTS: Composite scaffolds of 50% M. sarasinorum and X. testudinaria increased the cell viability and supported the cell growth within 14 days, whereas the osteogenic differentiation can be seen by the presence of collagen type 1 in day 12 based on immunocytochemistry result.CONCLUSION: The biosilica scaffolds from PCL+50% M. sarasinorum and PCL+50% X. testudinaria were promising 3D scaffolds for potential application in bone tissue engineering. In conclusion, this study shows evidence for the osteogenic differentiation of hWJ-MSC, which might be developed for bone tissue engineering.KEYWORDS: sponge, biosilica, scaffold, osteogenesis, stem cell","PeriodicalId":22516,"journal":{"name":"The Indonesian Biomedical Journal","volume":"162 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73745038","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Caffeic Acid Induces Apoptosis in MG-63 Osteosarcoma Cells via Protein Kinase C Delta (PKCδ) Translocation and Mitochondrial Membrane Potential Reduction 咖啡酸通过PKCδ易位和线粒体膜电位降低诱导MG-63骨肉瘤细胞凋亡

The Indonesian Biomedical Journal Pub Date : 2022-12-01 DOI: 10.18585/inabj.v14i4.2089

F. Sandra, M. Rizal, Caecilia Caroline Aliwarga, Jenifer Christy Hadimartana, M. Celinna

{"title":"Caffeic Acid Induces Apoptosis in MG-63 Osteosarcoma Cells via Protein Kinase C Delta (PKCδ) Translocation and Mitochondrial Membrane Potential Reduction","authors":"F. Sandra, M. Rizal, Caecilia Caroline Aliwarga, Jenifer Christy Hadimartana, M. Celinna","doi":"10.18585/inabj.v14i4.2089","DOIUrl":"https://doi.org/10.18585/inabj.v14i4.2089","url":null,"abstract":"BACKGROUND: Caffeic acid has been reported to activate caspases in MG-63 osteosarcoma cells, which can lead to apoptosis via both extrinsic and intrinsic apoptotic pathways. Translocation of protein kinase C delta (PKCδ), which reduces mitochondrial membrane potential (ΔΨm), is involved in apoptosis. The role of PKCδ translocation and ΔΨm alteration in caffeic acid-induced MG-63 cell apoptosis are largely unknown. Present study investigated the effect of caffeic acid on PKCδ translocation and ΔΨm in MG-63 cells.METHODS: MG-63 cells were cultured and starved, followed by pretreatment with or without Z-VAD-FMK and treatment with or without 10 μg/mL caffeic acid. MG-63 cells were collected, lysed, and processed to obtain cytosolic and mitochondrial fractions. Each fraction was subjected to immunoblotting analysis by using anti-PKCδ antibody. Mitochondrial membrane potential (ΔΨm) was measured using flow cytometry.RESULTS: Cytosolic PKCδ levels were higher than mitochondrial PKCδ levels in untreated and 1 h caffeic acid treatment groups. Inversely, cytosolic PKCδ levels were lower than the mitochondrial PKCδ levels after 6 and 12 h caffeic acid treatment. By Z-VAD-FMK pretreatment, cytosolic PKCδ levels were higher than mitochondrial PKCδ after 6 and 12 h caffeic acid treatment. After 6 h treatment with caffeic acid, ΔΨm was slightly shifted. More shifting occurred in MG-63 cells treated with caffeic acid for 12 h. The ΔΨm shifting was inhibited by Z-VAD-FMK pretreatment.CONCLUSION: Caffeic acid could trigger apoptosis of MG-63 osteosarcoma cells by inducing PKCδ translocation to mitochondria and reducing ΔΨm, which might cause MMP.KEYWORDS: caffeic acid, MG-63, osteosarcoma, PKCδ, mitochondrial membrane potential, mitochondrial membrane permeabilization, Z-VAD-FMK","PeriodicalId":22516,"journal":{"name":"The Indonesian Biomedical Journal","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79938322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Targeting Metastatic Cancer: Disseminated Tumor Cells and Premetastatic Niches 靶向转移性癌症:播散性肿瘤细胞和转移前龛

The Indonesian Biomedical Journal Pub Date : 2022-12-01 DOI: 10.18585/inabj.v14i4.2035

A. Meiliana, Nurrani Mustika Dewi, A. Wijaya

{"title":"Targeting Metastatic Cancer: Disseminated Tumor Cells and Premetastatic Niches","authors":"A. Meiliana, Nurrani Mustika Dewi, A. Wijaya","doi":"10.18585/inabj.v14i4.2035","DOIUrl":"https://doi.org/10.18585/inabj.v14i4.2035","url":null,"abstract":"BACKGROUND: Metastases are simply known as cancers spread to another part of the body, and often be responsible for the severity of cancer prognosis. Somehow, the complex mechanisms of metastases are not fully understood yet.CONTENT: The characteristic of cancer is akin to a never-healing wound. Cancer cells are plastic and dynamic as they build their niches and developed into metastases, even when they seem dormant. Therefore, cancer cells can survive the immune system. Recent research has shown the distinct biology of metastasis-initiating cell, which leads to tumor development in distant organs, immune surveillance evasion, and co-option of metastatic micro-environments. Effective cancer therapies must consider the regenerative states of metastatic malignancies and have careful observation of patient phenotypes.SUMMARY: This review aimed to provide an insight on genesis and characteristics of metastases, starting from its seeding and dormancy, until the advance phase. Thus, developing therapy for cancer metastases should not start as it grows, but even as earlier strategies since the primary tumor was detected.KEYWORDS: cancer metastasis, DTC, CTC, CSC, dormancy, pre-metastatic niche, plasticity","PeriodicalId":22516,"journal":{"name":"The Indonesian Biomedical Journal","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80261053","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Combination of Ursodeoxycholic Acid and Glutathione Improves Intestinal Morphology in Cholestasis by Downregulating TNF-α Expression 熊去氧胆酸和谷胱甘肽联用通过下调TNF-α表达改善胆汁淤积症患者肠道形态

The Indonesian Biomedical Journal Pub Date : 2022-12-01 DOI: 10.18585/inabj.v14i4.2044

J. A. N. Halim, E. Lestari, Sigit Adi Prasetyo, M. Muniroh, Agung Prasetyo

{"title":"Combination of Ursodeoxycholic Acid and Glutathione Improves Intestinal Morphology in Cholestasis by Downregulating TNF-α Expression","authors":"J. A. N. Halim, E. Lestari, Sigit Adi Prasetyo, M. Muniroh, Agung Prasetyo","doi":"10.18585/inabj.v14i4.2044","DOIUrl":"https://doi.org/10.18585/inabj.v14i4.2044","url":null,"abstract":"BACKGROUND: Cholestasis caused by obstruction of the common bile duct and may developed gut-derived sepsis due to reactive oxygen species (ROS) accumulation. Ursodeoxycholic acid (UDCA) and glutathione are widely known for their antioxidant properties, that might be beneficial against ROS. However, the effects of UDCA-glutathione combination against ROS have not been well elucidated in previous studies. Thus, this study was conducted to evaluate tumor necrosis factor (TNF)-α level and height of terminal ileal mucosal villus after UDCA-glutathione administration in cholestasis rat model.METHODS: Twenty-eight male Sprague Dawley rats were randomly grouped into four treatment groups, each group consisted of seven rats that had previously undergone bile duct ligation. Three groups received treatment of UDCA-glutathione combination on stratified dose, while the other one only received UDCA. Each treatment was given for 21 days. Ileal samples were collected from the rats and stained with mouse anti TNF-a antibody and hematoxylin-eosin (HE). Immunohistochemistry and histopathological examination were done using microscope and then calculated with ImageJ.RESULTS: The combination of UDCA and glutathione treatment decreased the TNF-α expression (p<0.05) compared to UDCA only group, particularly in group that received 20 mg UDCA and 15 mg glutathione supplementation (p<0.05) and group that received 30 mg UDCA and 20 mg glutathione supplementation (p<0.05). The height of the mucosa villous was higher in the UDCA-glutathione combination groups for all the three dosage variations given (p<0.05) compared to UDCA only group.CONCLUSION: UDCA-glutathione combination downregulates TNF-α expression and improves ileum mucosal villus height in cholestasis.KEYWORDS: cholestasis, glutathione, intestinal villus height, TNF-α, UDCA ","PeriodicalId":22516,"journal":{"name":"The Indonesian Biomedical Journal","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87132826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High Keratin Secretion of T47D Cell under Hypoxic Condition 缺氧条件下T47D细胞高角蛋白分泌的研究

The Indonesian Biomedical Journal Pub Date : 2022-12-01 DOI: 10.18585/inabj.v14i4.1999

A. Annisa, E. Herningtyas, D. Purnomosari

{"title":"High Keratin Secretion of T47D Cell under Hypoxic Condition","authors":"A. Annisa, E. Herningtyas, D. Purnomosari","doi":"10.18585/inabj.v14i4.1999","DOIUrl":"https://doi.org/10.18585/inabj.v14i4.1999","url":null,"abstract":"BACKGROUND: Hypoxia adaptation in cancer cells is mediated by hypoxia inducible factor (HIF)-1 that induces the expression of various proteins. Up to date, the analysis of T47D breast cancer cells-secreted protein is still limited. The aim of this study was to compare the protein profile secreted by T47D breast cancer cells under hypoxic and to normoxic conditions and identify the proteins as candidate for hypoxia marker proteins in T47D breast cancer cells.METHODS: T47D breast cancer cells were cultured under standard conditions. Cells were subcultured in normoxic and hypoxic conditions. The normoxic group was incubated with 20% oxygen and the hypoxic groups were incubated in a hypoxic chamber with 0.5% and 5% oxygen for 6, 24, and 48 hours in serum free medium. Proteins in the culture media were isolated and precipitated. Protein concentrations released by the cells were then measured by bicinchoninic assay (BCA). Protein bands were visualized by the sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) method. The bands that had differences between the hypoxic and normoxic groups were further analyzed by liquid chromatography-mass spectrophotometer (LC-MS).RESULTS: Hypoxic groups showed higher secretory protein than normoxic group. Protein bands were found in the 0.5% hypoxic group with a size of 50-75 kDa. Secretory proteins identified by LC-MS were keratin 1, 2, 9, and 10.CONCLUSION: The T47D cell line under 0.5% hypoxic treatment showed higher secretory proteins that identified as keratin 1, 2, 9, and 10.KEYWORDS: hypoxia, secretory protein, liquid chromatography-mass spectrophotometer","PeriodicalId":22516,"journal":{"name":"The Indonesian Biomedical Journal","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80946896","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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