The Indonesian Biomedical Journal最新文献_第9页

Paneth Cell Hyperplasia and Metaplasia in Hirschsprung-associated Enterocolitis in An Aganglionosis Rat Model 腺瘤病大鼠巨结肠相关性小肠结肠炎的Paneth细胞增生和化生

The Indonesian Biomedical Journal Pub Date : 2022-12-01 DOI: 10.18585/inabj.v14i4.2007

I. R. Budianto, A. Firmansyah, Y. Moenadjat, A. A. Jusuf, V. Soetikno

{"title":"Paneth Cell Hyperplasia and Metaplasia in Hirschsprung-associated Enterocolitis in An Aganglionosis Rat Model","authors":"I. R. Budianto, A. Firmansyah, Y. Moenadjat, A. A. Jusuf, V. Soetikno","doi":"10.18585/inabj.v14i4.2007","DOIUrl":"https://doi.org/10.18585/inabj.v14i4.2007","url":null,"abstract":"BACKGROUND: Many hypotheses regarding the pathophysiology of enterocolitis in aganglionic megacolon or Hirschsprung disease (HSCR) has been proposed. Paneth cells are columnar intestinal epithelial cells that have an important role in maintaining of intestinal homeostasis as a bactericide. Since enterocolitis in HSCR may have association with Paneth cells metaplasia and hyperplasia, current study investigated Paneth cells metaplasia and hyperplasia in the sigmoid colon of HSCR rat model and its products, namely a-defensins and IL-1b, in the sigmoid colon tissues.METHODS: Aganglionosis-induced and control Sprague-Dawley rats were euthanized on Day (D)-7, -14, -17, -19, -21, -23, -25, and -28. Sigmoid colon tissue was isolated at each time point, and degree of enterocolitis as well as Paneth cells metaplasia and hyperplasia were analyzed by Hematoxylin-eosin staining, then protein levels of a-defensins and interleukin (IL)-1b were determined by enzyme-linked immunosorbent assay (ELISA).RESULTS: Enterocolitis scores increased with time. The Paneth cells metaplasia and hyperplasia were observed on D14 until D28 (p<0.01 vs. control group) followed by an increased in the levels of IL-1b. The levels of a-defensins protein expression were initially increased (D7-D14; p<0.01 vs. control group) but then undergo reciprocal changes on D19 until D28 (p<0.01 vs. D7 and D14). Positive correlations between the degree of enterocolitis and Paneth cells number were detected in the sigmoid colon (r=0.42).CONCLUSION: Paneth cells underwent metaplasia and hyperplasia in the sigmoid colon of HSCR rats corresponding to an increase in the degree of enterocolitis, but not followed by an increase in the level of a-defensins as well as IL-1b, suggesting that there is an involvement of Paneth cells in the pathophysiology of enterocolitis due to HSCR.KEYWORDS: Hirschsprung, enterocolitis, defensins; metaplasia, Paneth cell, animal model","PeriodicalId":22516,"journal":{"name":"The Indonesian Biomedical Journal","volume":"41 2","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72576881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Rosemary and CoQ10 Alleviated the Detrimental Effects of Concomitant Administration of Acetaminophen and Carbamazepine by Accelerating Their Metabolism and Elimination 迷迭香和辅酶q10通过加速对乙酰氨基酚和卡马西平的代谢和消除，减轻了对乙酰氨基酚和卡马西平同时服用的有害影响

The Indonesian Biomedical Journal Pub Date : 2022-12-01 DOI: 10.18585/inabj.v14i4.1984

Marwa Magdy Hamido, N. Zaki, S. Nasr, W. El-Sayed

{"title":"Rosemary and CoQ10 Alleviated the Detrimental Effects of Concomitant Administration of Acetaminophen and Carbamazepine by Accelerating Their Metabolism and Elimination","authors":"Marwa Magdy Hamido, N. Zaki, S. Nasr, W. El-Sayed","doi":"10.18585/inabj.v14i4.1984","DOIUrl":"https://doi.org/10.18585/inabj.v14i4.1984","url":null,"abstract":"BACKGROUND: The interaction of carbamazepine (CBZ) and acetaminophen (APAP) could result in hepatic failure and mortality. This study was conducted to analyzed the potential of rosemary ethanol extract (REE) or coenzyme Q10 (CoQ10) to alleviate the interactions between CBZ and APAP.METHODS: Fourty-eight adult male rats were treated differently based on the assigned groups. Oxidative stress parameters, including malondialdehyde (MDA), glutathione (GSH), glutathione peroxidase (GPx), superoxide dismutase (SOD), catalase, and glutathione S-transferase (GST), and the expression levels of CYP3A4, CYP2E1, IL-6, TNF-α, and IL-1B in the liver were estimated. In addition, the histopathology of liver was examined and the plasma clearance rate of CBZ and APAP was estimated.RESULTS: Combination of CBZ and APAP significantly elevated alanine aminotransferase (ALT) activity and hepatic MDA, and reduced the activities of GPx, GST, and GSH level in liver. The gene expression of CYP3A4 and CYP2E1 was upregulated by CBZ and CoQ10, respectively. The expression of IL-6 has decreased in the groups treated with CBZ alone or in combination with APAP. TNF-α expression was significantly downregulated in the groups treated with CBZ, APAP, REE, CoQ10, or combination CBZ and APAP. The liver from CBZ and APAP combination group showed centrolobular degeneration and necrosis. REE and CoQ10 were able to alleviate most of these detrimental effects. The combined administration of CBZ and APAP extended the plasma clearance time of APAP and CBZ from 6 to 24 and from 9 to 24 hours, respectively.CONCLUSION: REE and CoQ10 alleviated the detrimental effects of the combination of CBZ and APAP through enhancing the cellular antioxidant milieu, induction of metabolizing enzymes, reduction of the plasma half-life of APAP and CBZ preventing their accumulation and potential interaction.KEYWORDS: acetaminophen, antioxidants, carbamazepine, CoQ10, CYP3A4, CYP2E1, glutathione, lipid peroxidation, rosemary","PeriodicalId":22516,"journal":{"name":"The Indonesian Biomedical Journal","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91115548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Anti-proliferative and Apoptotic Activities of Kasturi Tobacco (Nicotiana tabacum L.) Leaf Resinoid on Cervical Cancer Cell 卡斯图里烟草的抗增殖和细胞凋亡活性子宫颈癌细胞的叶树脂样物质

The Indonesian Biomedical Journal Pub Date : 2022-12-01 DOI: 10.18585/inabj.v14i4.2027

B. Kusumawardani, Larissa Tania, A. S. Nugraha

{"title":"Anti-proliferative and Apoptotic Activities of Kasturi Tobacco (Nicotiana tabacum L.) Leaf Resinoid on Cervical Cancer Cell","authors":"B. Kusumawardani, Larissa Tania, A. S. Nugraha","doi":"10.18585/inabj.v14i4.2027","DOIUrl":"https://doi.org/10.18585/inabj.v14i4.2027","url":null,"abstract":"BACKGROUND: Cervical cancer has a high rate of morbidity and mortality in women with cancer. Recent studies have found that tobacco (Nicotiana tabacum L.) is a potential source of anti-cancer agents. Hence, this study was conducted to determine the potential of Kasturi tobacco leaf resinoids as apoptotic agents against cervical cell malignancies, since it has not been fully elucidated before.METHODS: The phytochemical diversity of Kasturi tobacco resinoids was generated by gas chromatography-mass spectrometry (GC-MS) analysis followed by spectral similarity to National Institute of Standards and Technology (NIST) database. Cytotoxicity and proliferative activity of HeLa cells treated with Kasturi tobacco resinoids at various concentrations were evaluated by MTT assay. The expression of Caspase-3, cyclooxygenase-2 (COX-2) and heat shock protein 90 (HSP-90) in HeLa cells was analyzed by immunocytochemistry. Next, the migration ability of HeLa cells was observed by the scratch method.RESULTS: Kasturi tobacco resin contains 4,8,13-cyclotetradecatriene-1,3-diol, 1,5,9-trim with α-2,7,11-cembratriene-4,6-diol (α-CBD) structure in the form of a diterpenoid compound with the chemical formula C20H34O2 and a molecular weight of 306 Da. Kasturi tobacco resinoid with IC50 value of 2500 μg/mL inhibited proliferative activity during 72 hours. At a concentration of 1¼ IC50 and incubation for 48 hours, Caspase-3 expression increased by 74.1%, while COX-2 and HSP-90 expression decreased by 28.3% and 26.1%, respectively. HeLa cell migration was inhibited by Kasturi tobacco resinoid at 24 hours incubation.CONCLUSION: Kasturi tobacco resinoids with a concentration of 1¼ IC50 have potential as cervical anti-cancer agents by increasing Caspase-3 expression and decreasing COX-2 and HSP-90 expression within 48 hours.KEYWORDS: Kasturi tobacco resinoids, cervical cancer, anti-cancer agent, proliferative activity ","PeriodicalId":22516,"journal":{"name":"The Indonesian Biomedical Journal","volume":"54 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80174968","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Caffeic Acid Induces Intrinsic Apoptotic Pathway in MG-63 Osteosarcoma Cells Through Bid Truncation and Cytochrome c Release 咖啡酸诱导MG-63骨肉瘤细胞内生性凋亡通路及细胞色素c释放

The Indonesian Biomedical Journal Pub Date : 2022-09-08 DOI: 10.18585/inabj.v14i3.2032

F. Sandra, M. Rizal, Ayasha Hajjar Audreyandra Wahid, Monica Andajana, M. Celinna

{"title":"Caffeic Acid Induces Intrinsic Apoptotic Pathway in MG-63 Osteosarcoma Cells Through Bid Truncation and Cytochrome c Release","authors":"F. Sandra, M. Rizal, Ayasha Hajjar Audreyandra Wahid, Monica Andajana, M. Celinna","doi":"10.18585/inabj.v14i3.2032","DOIUrl":"https://doi.org/10.18585/inabj.v14i3.2032","url":null,"abstract":"BACKGROUND: Caffeic acid has been reported to induce apoptosis in MG-63 osteosarcoma cells via caspases activation. However, apoptotic pathway that is involved in the caffeic acid-induced apoptosis is still unclear. Present study aimed to investigate the role of cytochrome c (Cyt c) release and BH3-interacting death (Bid) activation in caffeic acid-induced apoptosis in MG-63 osteosarcoma cells.METHODS: MG-63 cells were cultured, pretreated with/without Z-VAD FMK and treated with/without 10 μg/mL caffeic acid. Treated MG-63 cells were then lysed, homogenized, and processed further to prepare cell lysate and mitochondrial fraction. Immunoblotting method was used to measure the amount of Bid and truncated Bid (t-Bid) as well as mitochondrial and cytosolic Cyt c.RESULTS: The amount of Bid and mitochondrial Cyt c in MG-63 cells decreased in a time-dependent manner, while the amount of t-Bid and cytosolic Cyt c increased in a time-dependent manner. By pretreatment of 100 μM Z-VAD-FMK for 2 h, the amount of Bid and mitochondrial Cyt c was significantly higher, while the amount of t-Bid and cytosolic Cyt c was significantly lower after caffeic acid treatment for 6 and 12 h compared to MG-63 cells that were not pretreated.CONCLUSION: Caffeic acid could induce Cyt c release through the activation of Bid in MG-63 osteosarcoma cells.KEYWORDS: caffeic acid, osteosarcoma, MG-63 cells, Bid, t-Bid, cytochrome c, Z-VAD-FMK","PeriodicalId":22516,"journal":{"name":"The Indonesian Biomedical Journal","volume":"37 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74653225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Evaluations of Antibacterial Properties of Zingiber purpureum Essential Oil Against 13 Different Gram-positive and Gram-negative Bacteria 紫姜精油对13种革兰氏阳性菌和革兰氏阴性菌抑菌性能的评价

The Indonesian Biomedical Journal Pub Date : 2022-09-08 DOI: 10.18585/inabj.v14i3.1967

N. Tandirogang, S. Anitasari, E. Arung, S. Paramita, Yu Shen

{"title":"Evaluations of Antibacterial Properties of Zingiber purpureum Essential Oil Against 13 Different Gram-positive and Gram-negative Bacteria","authors":"N. Tandirogang, S. Anitasari, E. Arung, S. Paramita, Yu Shen","doi":"10.18585/inabj.v14i3.1967","DOIUrl":"https://doi.org/10.18585/inabj.v14i3.1967","url":null,"abstract":"BACKGROUND: Indonesia's tropical forest is home to around 80% of the world's medicinal plants. One of these is Zingiber purpureum, which have traditionally been used to treat joint discomfort, the common cold, and jaundice. The rhizomes of this plant have been suggested to possess antibacterial action in the treatment of infections. In this study, Z. purpureum was screened for antibacterial activity against 13 bacteria (Gram-positive and Gram-negative).METHODS: Z. purpureum rhizomes were obtained and the distillated extracts were made to generate essential oil. The minimum inhibitory concentration (MIC) and Kirby Bauer disk diffusion methods were used to determine the antibacterial activity.RESULTS: All bacteria activity were inhibited by the essential oil of Z. purpureum at concentrations ranging from 2.5 vol% to 10 vol%. However, several bacterias (Escherichia coli, Pseudomonas aeruginosa, and Enterobacter cloacae) were inhibited at the lowest concentration (0.63 vol %), with the inhibition zones ranging from 6.7 mm to 8.0 mm. Meanwhile, the widest inhibition zone (13.3 mm) was reported on E. cloacae at 10 vol% concentration.CONCLUSION: A 10 vol% Z. purpureum rhizome extract inhibits Gram-positive and Gram-negative bacteria, particularly those that are resistant to a variety of antibiotics.KEYWORDS: Zingiber purpureum, antibacterial agents, susceptibility test, infection","PeriodicalId":22516,"journal":{"name":"The Indonesian Biomedical Journal","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89826575","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

High NF-κB and RAGE Expression in Fetal Membrane of Premature Rupture of Membrane (PROM) Subject 膜早破(PROM)患者胎膜中NF-κB和RAGE的高表达

The Indonesian Biomedical Journal Pub Date : 2022-09-08 DOI: 10.18585/inabj.v14i3.1859

I. K. Sudiarta, Monicha Zalzabilla Aldinasyah, Cindy Jennilyn Candra, S. Supriyono, A. Rasyida

{"title":"High NF-κB and RAGE Expression in Fetal Membrane of Premature Rupture of Membrane (PROM) Subject","authors":"I. K. Sudiarta, Monicha Zalzabilla Aldinasyah, Cindy Jennilyn Candra, S. Supriyono, A. Rasyida","doi":"10.18585/inabj.v14i3.1859","DOIUrl":"https://doi.org/10.18585/inabj.v14i3.1859","url":null,"abstract":"BACKGROUND: Premature Rupture of Membranes (PROM) is significantly linked to the infections-related maternal deaths. In the inflammatory process, the influencing stressor will stimulate the activation of Nuclear Factor Kappa B (NF-κB) and Receptor of Advanced Gyclation End product (RAGE). Yet up to date, the expression of NF-κB and RAGE in pregnant women with PROM are still rarely studied. Therefore, this study aimed to observe the differences of NF-κB and RAGE expression from PROM and non-PROM subjects.METHODS: This was a cross-sectional study involving 20 PROM subjects and 20 non-PROM subjects with infections and complications. Samples from the fetal membrane tissue of subjects were obtained and put into paraffin block preparation for the determination of NF-κB and RAGE expression. The detection of NF-κB and RAGE expression was conducted using immunohistochemical staining and observed under an upright light microscope. The expressions were later calculated using ImageJ software. RESULTS: Both NF-κB and RAGE expression were found to be higher in PROM subjects compare to the non-PROM subjects. The median of NF-κB in PROM and non-PROM subjects were 32.47±1.22 and 5.59±1.09, respectively (p=0.000). While the median of RAGE in PROM subjects was 53.58±3.46, and in non-PROM subjects was 11.64±2.49 (p=0.013).CONCLUSION: There is significant difference between NF-κB and RAGE expression in fetal membranes of PROM and non-PROM subjects. Therefore, the increased of NF-κB and RAGE expression can be used as a potential marker to detect complication of PROM.KEYWORDS: premature ruptures of membrane, non-premature ruptures of membrane, expression of NF-κB ","PeriodicalId":22516,"journal":{"name":"The Indonesian Biomedical Journal","volume":"70 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85146813","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Role of Klotho G395A Gene Polymorphism in Atherosclerotic Cardiovascular Disease and Mortality Risk Scores in Non-dialysis Chronic Kidney Disease Klotho G395A基因多态性在非透析慢性肾病患者动脉粥样硬化性心血管疾病和死亡风险评分中的作用

The Indonesian Biomedical Journal Pub Date : 2022-09-08 DOI: 10.18585/inabj.v14i3.1975

H. Susilo, B. Pikir, M. Thaha, M. Y. Alsagaff, S. D. Suryantoro, C. Wungu, D. S. Budi, Laurentius Andre, Cennikon Pakpahan

{"title":"The Role of Klotho G395A Gene Polymorphism in Atherosclerotic Cardiovascular Disease and Mortality Risk Scores in Non-dialysis Chronic Kidney Disease","authors":"H. Susilo, B. Pikir, M. Thaha, M. Y. Alsagaff, S. D. Suryantoro, C. Wungu, D. S. Budi, Laurentius Andre, Cennikon Pakpahan","doi":"10.18585/inabj.v14i3.1975","DOIUrl":"https://doi.org/10.18585/inabj.v14i3.1975","url":null,"abstract":"BACKGROUND: Cardiovascular disease (CVD) is the leading cause of death in patients with chronic kidney disease (CKD). Klotho expression was reduced in patients with CKD, leading to vascular calcification, endothelial dysfunction, and atherosclerosis. We investigated the role of the klotho G395A gene polymorphism and plasma klotho level in the ten-year risk of atherosclerotic cardiovascular disease (ASCVD) and CVD mortality in CKD patients.METHODS: We used the PCR-CTPP assay method to genotype klotho G395A single nucleotide polymorphism (SNP) in 72 non-dialysis CKD patients. The klotho level was determined using the enzyme-linked immunoassay (ELISA) method. Path analysis was used to determine the relationship between the klotho G395A SNP, plasma klotho level, ASCVD risk score, and CVD mortality risk score.RESULTS: Our results showed that the GA genotype had lower plasma klotho levels than the GG genotype (path coefficient=-0.185, p=0.000). There was a significant negative correlation between plasma klotho level and the ASCVD risk score (r=-0.243, p=0.040), but no significant correlation was found between plasma klotho level and the CVD mortality risk score (r=-0.145, p=0.225). Path analysis showed that plasma klotho level had a significant negative direct effect on ASCVD risk score (path coefficient=-0.272, p=0.000) and an indirect effect on CVD mortality risk score (path coefficient=0.187, p=0.005).CONCLUSION: Klotho G395A SNP might reduce lower plasma klotho levels, which increased ASCVD and CVD mortality risk scores in non-dialysis CKD patients. However, other risk factors such as age, CKD stages, hypertension, and smoking should be taken into consideration. Therefore, large-scale genetic association studies with adjusted variables could be conducted in various ethnic groups for a more robust result.KEYWORDS: klotho, single nucleotide polymorphism, cardiovascular disease, chronic kidney disease","PeriodicalId":22516,"journal":{"name":"The Indonesian Biomedical Journal","volume":"183 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80464126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Insulin Resistance and Other Adipokines as Clinical Predictors of Gestational Diabetes Mellitus among Pregnant Women 胰岛素抵抗和其他脂肪因子作为妊娠期糖尿病的临床预测因子

The Indonesian Biomedical Journal Pub Date : 2022-09-08 DOI: 10.18585/inabj.v14i3.1934

Raghda Abdulsamad Abdualhay, A. J. Al-Fartosy

{"title":"Insulin Resistance and Other Adipokines as Clinical Predictors of Gestational Diabetes Mellitus among Pregnant Women","authors":"Raghda Abdulsamad Abdualhay, A. J. Al-Fartosy","doi":"10.18585/inabj.v14i3.1934","DOIUrl":"https://doi.org/10.18585/inabj.v14i3.1934","url":null,"abstract":"BACKGROUND: Gestational diabetes mellitus (GDM) has a strong relationship with an increased risk of maternal and perinatal complications. However, in Basrah, Iraq, studies regarding GDM are still limited. In current study, we aimed to investigate the association between insulin resistance and some clinical predictors of GDM among pregnant women in 1st and 3rd trimesters of gestation.METHODS: This case-control study was conducted on 44 pregnant women with GDM and 45 without GDM aged 20 to 40 years who applied for GDM screening during the first (9-13 week) and third trimester (24-28 week) of pregnancy. Demographics, blood glucose, HbA1c, insulin, homeostatic model assessment for insulin resistance (HOMA-IR), spexin, nesfatin-1, orexin-A, vaspin and lipid profile levels were compared between groups.RESULTS: Subjects with GDM showed a higher level of glucose, insulin HOMA-IR, HbA1c, spexin, vaspin in the first and third trimesters of pregnancy (p<0.01) compared to the healthy subjects. Meanwhile in the first and third trimester, subjects with GDM showed significantly lower level of nesfatin-1 and orexin-A compare to the control. In third trimester, oral glucose tolerance test (OGTT) outcomes for fasting glucose at 1 hour, 2 hours, and 3 hours after glucose load were significantly higher (p<0.01). According to the area under the receiver operating characteristics (ROC) curve (AUC) findings, HOMA-IR, spexin, and vaspin may be more effective predictors biomarkers for GDM in pregnant subjects, while orexin-A and nesfatin-1 were ineffective.CONCLUSION: The correlation of insulin resistance and adipokines in the first and thrid trimester was not significantly different, which may cast new light on the possible role as an etiological cause of GDM and might be a better monitoring parameter in women with GDM. KEYWORDS: gestational diabetes mellitus, insulin resistance, vaspin, spexin, orexin-A, nesfatin-1","PeriodicalId":22516,"journal":{"name":"The Indonesian Biomedical Journal","volume":"70 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87840874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Thymoquinone Modulates Local MMP-9, IL-10, and IgG in Sciatic Nerve Crush Injury Animal Model 百里醌对坐骨神经挤压损伤动物模型局部MMP-9、IL-10和IgG的调节作用

The Indonesian Biomedical Journal Pub Date : 2022-09-08 DOI: 10.18585/inabj.v14i3.2011

V. Besin, A. Bajamal, I. Sudiana, M. Agil, J. Nugraha, M. H. Machfoed, P. B. Notopuro, Naesilla Naesilla

{"title":"Thymoquinone Modulates Local MMP-9, IL-10, and IgG in Sciatic Nerve Crush Injury Animal Model","authors":"V. Besin, A. Bajamal, I. Sudiana, M. Agil, J. Nugraha, M. H. Machfoed, P. B. Notopuro, Naesilla Naesilla","doi":"10.18585/inabj.v14i3.2011","DOIUrl":"https://doi.org/10.18585/inabj.v14i3.2011","url":null,"abstract":"BACKGROUND: Interleukin (IL)-10 is involved in Wallerian degeneration after peripheral nerve crush injury. Oral thymoquinone was previously observed to decrease local immunoglobulin-G (IgG) in a crush-injured rat model. No study has evaluated the pathway of various thymoquinone dosages on local IgG and IL-10 in this injury.METHODS: This experimental study used 126 Rattus norvegicus Wistar rats that were divided into 18 groups: six groups received a placebo, the other six groups received thymoquinone at 100 mg/kg/day and the last six groups received thymoquinone at 250 mg/kg/day, respectively. Rats were sacrificed at 12, 18, 24, 5x24, 6x24, and 7x24 hours. Matrix metalloproteinase-9 (MMP-9), IL-10, and local IgG levels were assessed by Enzyme-Linked Immunosorbent Assay (ELISA). The nuclear factor KappaB (NF-κB) expressions on Schwann cells were examined by flow cytometry. Path analysis was performed using SmartPLS.RESULTS: The path analysis showed that 100mg/kg/day of thymoquinone significantly decreased NF-κB expression. However, NF-κB did not affect local MMP-9, and MMP-9 had no significant relationship with local IL-10 and IgG. Thymoquinone 250 mg/kg/day also significantly inhibited NF-kB expression, decreased local MMP-9, and, in turn, decreased local IL-10 and IgG.CONCLUSION: Administration of oral thymoquinone 250 mg/kg/day decreases local IgG and IL-10 levels via suppressing NF-κB expression and MMP-9 leveKEYWORDS: thymoquinone, crush injury, IgG, IL-10, MMP-9, NF-κB","PeriodicalId":22516,"journal":{"name":"The Indonesian Biomedical Journal","volume":"99 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80696470","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Increased Platelet-derived Microparticles Counts is Correlated with Elevated Blood LDL Cholesterol in Acute Myocardial Infarction 急性心肌梗死患者血小板源性微粒计数增加与血液LDL胆固醇升高相关

The Indonesian Biomedical Journal Pub Date : 2022-09-08 DOI: 10.18585/inabj.v14i3.1892

Kelvin Supriami, Ira Puspitawati, Dyah Samti Mayasari, A. B. Hartopo

{"title":"Increased Platelet-derived Microparticles Counts is Correlated with Elevated Blood LDL Cholesterol in Acute Myocardial Infarction","authors":"Kelvin Supriami, Ira Puspitawati, Dyah Samti Mayasari, A. B. Hartopo","doi":"10.18585/inabj.v14i3.1892","DOIUrl":"https://doi.org/10.18585/inabj.v14i3.1892","url":null,"abstract":"BACKGROUND: Platelet-derived microparticles (PDMPs) and low-density lipoprotein (LDL) cholesterol are contributing factors to acute myocardial infarction (AMI). However, the association between LDL cholesterol and PDMPs in AMI has not fully discovered. This study assessed the correlation between these two parameters in patients diagnosed with AMI.METHODS: This was an observational cross-sectional study involving 95 subjects with AMI. The blood measurement of PDMPs counts and LDL cholesterol levels were conducted concomitantly within 24 hours of admission. PDMPs count was analyzed by flow-cytometry method, meanwhile the LDL cholesterol was measured with enzymatic and colorimetric methods. For further analysis, subjects were further divided into LDL cholesterol level ≥130 mg/dL and <130 mg/dL. A statistical test was conducted for a correlative and comparative analyses.RESULTS: A correlative analysis to assess the association between PDMPs counts and LDL cholesterol level depicted a low but significant positive correlation (r=0.231, p=0.024). Furthermore, mean PDMPs counts was significantly higher in subjects with LDL cholesterol level ≥130 mg/dL compared to LDL cholesterol level <130 mg/dL (12,499.59 (95% CI: 8,507.44-16,491.74) counts/μL vs. 9,267.23 (95% CI: 4,445.45-14,089.01) counts/μL; p=0.039).CONCLUSION: There was a significant correlation between PDMPs counts and LDL cholesterol levels in AMI. A significantly increased PDMPs counts were found in subjects with LDL cholesterol level ≥130 mg/dL. Therefore, it is recommended to measure PDMPs in patients with high LDL cholesterol levels as both might be significant AMI biomarkers.KEYWORDS: acute myocardial infarction, LDL-cholesterol, platelet microparticles, platelet activation","PeriodicalId":22516,"journal":{"name":"The Indonesian Biomedical Journal","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"90251412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1