Anti-proliferative and Apoptotic Activities of Kasturi Tobacco (Nicotiana tabacum L.) Leaf Resinoid on Cervical Cancer Cell

The Indonesian Biomedical Journal Pub Date : 2022-12-01 DOI:10.18585/inabj.v14i4.2027

B. Kusumawardani, Larissa Tania, A. S. Nugraha

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Abstract

BACKGROUND: Cervical cancer has a high rate of morbidity and mortality in women with cancer. Recent studies have found that tobacco (Nicotiana tabacum L.) is a potential source of anti-cancer agents. Hence, this study was conducted to determine the potential of Kasturi tobacco leaf resinoids as apoptotic agents against cervical cell malignancies, since it has not been fully elucidated before.METHODS: The phytochemical diversity of Kasturi tobacco resinoids was generated by gas chromatography-mass spectrometry (GC-MS) analysis followed by spectral similarity to National Institute of Standards and Technology (NIST) database. Cytotoxicity and proliferative activity of HeLa cells treated with Kasturi tobacco resinoids at various concentrations were evaluated by MTT assay. The expression of Caspase-3, cyclooxygenase-2 (COX-2) and heat shock protein 90 (HSP-90) in HeLa cells was analyzed by immunocytochemistry. Next, the migration ability of HeLa cells was observed by the scratch method.RESULTS: Kasturi tobacco resin contains 4,8,13-cyclotetradecatriene-1,3-diol, 1,5,9-trim with α-2,7,11-cembratriene-4,6-diol (α-CBD) structure in the form of a diterpenoid compound with the chemical formula C20H34O2 and a molecular weight of 306 Da. Kasturi tobacco resinoid with IC50 value of 2500 μg/mL inhibited proliferative activity during 72 hours. At a concentration of 1¼ IC50 and incubation for 48 hours, Caspase-3 expression increased by 74.1%, while COX-2 and HSP-90 expression decreased by 28.3% and 26.1%, respectively. HeLa cell migration was inhibited by Kasturi tobacco resinoid at 24 hours incubation.CONCLUSION: Kasturi tobacco resinoids with a concentration of 1¼ IC50 have potential as cervical anti-cancer agents by increasing Caspase-3 expression and decreasing COX-2 and HSP-90 expression within 48 hours.KEYWORDS: Kasturi tobacco resinoids, cervical cancer, anti-cancer agent, proliferative activity

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卡斯图里烟草的抗增殖和细胞凋亡活性子宫颈癌细胞的叶树脂样物质

背景:宫颈癌在女性癌症患者中发病率和死亡率都很高。最近的研究发现烟草(Nicotiana tabacum L.)是抗癌剂的潜在来源。因此，本研究旨在确定香树烟叶树脂作为抗宫颈细胞恶性肿瘤的凋亡药物的潜力，因为它之前没有被完全阐明。方法:采用气相色谱-质谱法(GC-MS)对卡斯图里烟草树脂进行植物化学多样性分析，并与美国国家标准与技术研究院(NIST)数据库进行光谱相似性分析。采用MTT法对不同浓度的烟草树脂处理HeLa细胞的细胞毒性和增殖活性进行了研究。采用免疫细胞化学方法检测Caspase-3、环氧合酶-2 (COX-2)和热休克蛋白90 (HSP-90)在HeLa细胞中的表达。其次，采用划痕法观察HeLa细胞的迁移能力。结果:烟香树脂含有4,8,13-环十四烯-1,3-二醇，1,5,9-邻环十四烯-1,3-二醇(α-CBD)，为二萜类化合物，化学式为C20H34O2，分子量为306 Da。烟叶树脂的IC50值为2500 μg/mL，在72 h内抑制了烟叶的增殖活性。在1¼IC50浓度下，孵育48 h, Caspase-3表达量增加74.1%，COX-2和HSP-90表达量分别下降28.3%和26.1%。Kasturi烟草树脂可抑制HeLa细胞在24小时内的迁移。结论:1¼IC50浓度的卡斯图里烟草树脂可在48h内提高Caspase-3的表达，降低COX-2和HSP-90的表达，具有潜在的宫颈癌抗癌作用。关键词:卡斯图里烟草树脂，宫颈癌，抗癌剂，增殖活性

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The Indonesian Biomedical Journal

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