Chinese Journal of Nuclear Mdeicine最新文献_第2页

~(125)I-β-CIT imaging study of striatal dopamine transporters in mice model of Parkinsonism 帕金森模型小鼠纹状体多巴胺转运体的~(125)I-β-CIT成像研究

Chinese Journal of Nuclear Mdeicine Pub Date : 2001-03-25 DOI: 10.3760/CMA.J.ISSN.0253-9780.2001.02.008

Weng Zhongfang

{"title":"~(125)I-β-CIT imaging study of striatal dopamine transporters in mice model of Parkinsonism","authors":"Weng Zhongfang","doi":"10.3760/CMA.J.ISSN.0253-9780.2001.02.008","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0253-9780.2001.02.008","url":null,"abstract":"Objective To detect the activity of striatal dopamine transporters (DAT) in lesions of different order of severity of MPTP-induced mice model of parkinsonism by autoradiography with 125I-β-CIT and to evaluate the clinical use of the β-CIT imaging for DAT detection. Methods With regard to the different duration (days) of MPTP treatment, the C57BL mice were randomly divided into 5 groups, that is MPTP 1,3,5 and 7day groups and control group treated with normal saline instead of MPTP. Two hours after intravenous administration with 125I-β-CIT of 148 kBq, the brain tissue sections were imaged by autoradiography. The levels of dopamine (DA) and its metabolites were measured by high performance liquid chromatography and electrochemical detection (HPLC-ECD). The tyrosine hydroxylase (TH)-positive cells and fibres in the substantia nigra and striatum of the mice were observed by means of immunohistochemical technique. Results As compared with control group, the radioactivity ratios of striatum to cortex (ST/CX) in 4 MPTP-treated groups were significantly reduced,by 20%, 42%, 45% and 52%,respectively. The concentrations of DA in the striatum of 4 MPTP-treated groups were remarkably decreased,by 47%, 75%, 95% and 95%,respectively. The gradual loss of DA neurons and fibres in the substantia nigra and striatum in 4 MPTP-treated groups was observed under microscopy. Conclusions The functional abnormality of DAT paralleled the changes observed in neurochemistry and neuropathology studies in the lesions of different order of injury of the MPTP-treated mice. The β-CIT scanning for the activity of DAT may be useful for diagnosing PD at earlier phase and for monitoring the progression of the disease.","PeriodicalId":221169,"journal":{"name":"Chinese Journal of Nuclear Mdeicine","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2001-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"129519742","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A study of the biological effects of rare earth elements at cellular level using nuclear techniques 利用核技术研究稀土元素在细胞水平上的生物效应

Chinese Journal of Nuclear Mdeicine Pub Date : 2001-03-25 DOI: 10.3760/CMA.J.ISSN.0253-9780.2001.02.023

P. Zhao, Xiangrui Ji, Hua Zhang, Tianfu Jiang

{"title":"A study of the biological effects of rare earth elements at cellular level using nuclear techniques","authors":"P. Zhao, Xiangrui Ji, Hua Zhang, Tianfu Jiang","doi":"10.3760/CMA.J.ISSN.0253-9780.2001.02.023","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0253-9780.2001.02.023","url":null,"abstract":"Objective To investigate the biological effects and the effecting mechanisms of rare earth elements La,Gd and Ce on cultured rat cells.Methods The biological effects of La 3+ on cultured rat cells and the subcellular distribution of La and Gd and Ce, and the inflow of 45Ca 2+ into the cells and total cellular calcium were measured by isotopic tracing, Proton Induced X Ray Emission Analysis (PIXE) and the techniques of biochemistry and cellular biology.Results La 3+ at the concentration of 10 -10(or 10 -9)～10 -6mol/L significantly increased quantity of incorporation of 3H-TdR into DNA, total cellular protein and the activity of succinic dehydrogenase of mitochondria. The cell cycle analysis showed that the proportions of cells in S phase were accordingly increased acted by La 3+ at above range of concentration. But these values were significantly decreased when concentration of La 3+ raised to 10 -4～10 -3mol/L. It was further discovered that La,Gd,and Ce distributed mostly in the nuclei, and then in membranes. Gd and Ce also promoted the inflow of 45Ca 2+ into the cells and increased the total calcium content in cells.Conclusions ① La 3+ at a wide concentration range of 10 -10( or 10 -9)～10 -6mol/L promotes proliferation of cultured rat cells, but at even higher concentration (10 -4～10 -3mol/L) shows cellular toxicity, and there is a striking dose-effect relationship. ② La,Gd and Ce can enter the cells and mainly distribute in the nuclei. ③ Gd and Ce can promote the inflow of extracellular Ca 2+ into the cells and increase total cellular calcium.","PeriodicalId":221169,"journal":{"name":"Chinese Journal of Nuclear Mdeicine","volume":"203 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2001-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"121028223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

A clinical comparative study of 99Tcm-tetrofosmin with 99Tcm-MIBI in breast carcinoma and axillary lymph node metastases 99Tcm-tetrofosmin与99Tcm-MIBI治疗乳腺癌及腋窝淋巴结转移的临床比较研究

Chinese Journal of Nuclear Mdeicine Pub Date : 2001-03-25 DOI: 10.3760/CMA.J.ISSN.0253-9780.2001.02.015

Huang Weikun, Liang Guoxin, Qin Delin

{"title":"A clinical comparative study of 99Tcm-tetrofosmin with 99Tcm-MIBI in breast carcinoma and axillary lymph node metastases","authors":"Huang Weikun, Liang Guoxin, Qin Delin","doi":"10.3760/CMA.J.ISSN.0253-9780.2001.02.015","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0253-9780.2001.02.015","url":null,"abstract":"Objective To evaluate the clinical diagnostic value of 99Tcm-tetrofosmin in the imaging of breast carcinoma and axillary lymph node metastases and to compare with that of 99Tcm-MIBI Methods 52 females with breast masses were examined by 99Tcm-tetrofosmin scintigraphy and 99Tcm-MIBI scintigraphyAll the results were compared with that from pathologic study Results 26 patients were found to have a primary malignancy of the breast ,whereas 30 had benign disease 99Tcm-tetrofosmin breast imaging showed abnormal uptake in 21 of 26 malignancies and in 11 of 16 axillary lymph node metastasesThe sensitivity ,specificity, and accuracy values obtained with 99Tcm-tetrofosmin and 99Tcm-MIBI scintigraphy for breast carcinoma were 808%, 767%, 786%,and 731%,733%,732%, respectively The values obtained with 99Tcm-tetrofosmin and 99Tcm-MIBI scintigraphy for axillary lymph node metastases were 688%, 800%, 760%,and 750%,800%,769%, respectively Conclusions Both of the two techniques are effective in the differentiation of malignant breast mass-es from benign ones and in detecting axillary lymph node metastasesHowever , 99Tcm-tetrofosmin is superior to 99Tcm-MIBI in detecting breast carcinoma","PeriodicalId":221169,"journal":{"name":"Chinese Journal of Nuclear Mdeicine","volume":"48 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2001-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"124700294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Preliminary clinical application of radioimmunoimaging of breast cancer using ~(99)Tc~m labeled anti-mucin monoclonal antibody ~(99)Tc~m标记抗粘蛋白单克隆抗体在乳腺癌放射免疫显像中的初步临床应用

Chinese Journal of Nuclear Mdeicine Pub Date : 2000-03-25 DOI: 10.3760/CMA.J.ISSN.0253-9780.2000.02.009

Haorong Wu, Jinchang Wu

{"title":"Preliminary clinical application of radioimmunoimaging of breast cancer using ~(99)Tc~m labeled anti-mucin monoclonal antibody","authors":"Haorong Wu, Jinchang Wu","doi":"10.3760/CMA.J.ISSN.0253-9780.2000.02.009","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0253-9780.2000.02.009","url":null,"abstract":"Objective To evaluate the clinical value of radioimmunoimaging (RII) in diagnosis of breast cancer using 99Tcm labeled antimucin monoclonal antibody. Methods 17 patients with various breast tumors underwent RII. Results 8 of 9 patients with primary breast cancer gave positive results of RII, with the sensitivity of 88.89%. Ipsilateral lymphatic metastasis was detected in 4 of 5 patients with primary breast cancer plus axillary lymph node involvement and for the remaining 1 patient contralateral lymph node metastasis was detected; the patient received mastectomy 5 years ago due to breast cancer of that side. RII results of the remaining 7 patients were negative, they were 6 patients with benign breast tumour and 1 followedup patient who underwent mastectomy 7 years ago due to breast cancer. Conclusions RII using 99Tcm labeled antimucin McAb can be used as a valuable method in the diagnosis of primary breast cancer, lymph node metastases and recurrence. \u0000 \u0000Key words: \u0000Antibodies, monoclonal; Antibodies, neoplasm; Mucins; Breast neoplasms; Radioimmunodetection","PeriodicalId":221169,"journal":{"name":"Chinese Journal of Nuclear Mdeicine","volume":"64 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2000-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130287070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Application of radionuclide imaging in osteosarcoma 放射性核素显像在骨肉瘤中的应用

Chinese Journal of Nuclear Mdeicine Pub Date : 2000-03-25 DOI: 10.3760/CMA.J.ISSN.0253-9780.2000.02.006

Peng Jingjing, Z. Qing, Cai Youbo, Li Yutao, Hao Lin, Niu Xiaohui, He Hai-yan

{"title":"Application of radionuclide imaging in osteosarcoma","authors":"Peng Jingjing, Z. Qing, Cai Youbo, Li Yutao, Hao Lin, Niu Xiaohui, He Hai-yan","doi":"10.3760/CMA.J.ISSN.0253-9780.2000.02.006","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0253-9780.2000.02.006","url":null,"abstract":"Objective In order to diagnose osteosarcoma, examine multicentric osteosarcoma and assess the effect of treatment, we evaluate the characters of radionuclide imaging and uptake of 99TcmMDP and 201Tl in osteosarcoma Methods 133 patients with osteosarcoma underwent a total of 174 times of 99TcmMDP wholebody scintigraphic imaging and 33 times of 201Tl local imaging to observe the characters of imaging of osteosarcoma and the uptake of imaging agents Results The character of 99TcmMDP imaging was the high uptake in the lesion of 108 patients with monolesion osteosarcoma There were some areas of reduced uptake in the hot zone with or without uptake in soft tissues Semiquantitative uptake ratio of the lesion to its correspondent tissue at opposite side was 540±342 (n=33) 201Tl imaging in 33 patients with osteosarcoma: blood flow was distinctly increased The uptake ratio at 15 min was 338±212, and at 3 h was 203±087 (n=33) The 99TcmMDP imaging in patients with multiple osteosarcoma and patients with multiple pulmonary metastasis, and the application of 99TcmMDP imaging in recurrence and bone metastasis of osteosarcoma were also reported Conclusions The biologic behavior of osteosarcoma can be illustrated by radionuclide imaging It will play an important role in the basic and clinical research of primary osteosarcoma \u0000 \u0000Key words: \u0000Osteosarcoma; Radionuclide imaging; MDP","PeriodicalId":221169,"journal":{"name":"Chinese Journal of Nuclear Mdeicine","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2000-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"122676920","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Comparative studies of D_2 receptors and cerebral blood flow in hemi-Parkinsonism rats 半帕金森大鼠D_2受体与脑血流的比较研究

Chinese Journal of Nuclear Mdeicine Pub Date : 2000-03-25 DOI: 10.3760/CMA.J.ISSN.0253-9780.2000.02.004

Lin Xiang-tong

{"title":"Comparative studies of D_2 receptors and cerebral blood flow in hemi-Parkinsonism rats","authors":"Lin Xiang-tong","doi":"10.3760/CMA.J.ISSN.0253-9780.2000.02.004","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0253-9780.2000.02.004","url":null,"abstract":"Objective To study the relationship between dopamine D 2 receptors and cerebral blood flow in hemi Parkinsonism rats Methods Hemi Parkinsonism rats were made by stereotaxic 6 hydroxy dopamine (6 OH DA) lesions in substantia nigra and ventral tegmental area, apomorphine (Apo) which could induce the successful model rat to rotate toward the intact side was used to select the rat models, 125 I IBZM in vivo autoradiography and 99 Tc m HMPAO regional cerebral biodistribution analysis were used to study D 2 receptors and cerebral blood flow The HPLC ECD was used to measure striatum DA and its metabolite content Results The lesioned side striatum DA and its metabolites homovanillic acid (HVA) 3,4 dihydroxyphenylacetic acid (DOPAC) reduced significantly than that of the intact side and pseudo operated group, striatum/cerebellum 125 I IBZM uptake ratio was 8 04±0 71 in lesioned side of hemi Parkinsonism rats, significantly increased compared with the intact side and the pseudo operated group (P0 05), (30 11±4 53)% of enhancement as compared to the intact side, and also showed good correlation with Apo induced rotation numbers within 30 min (r=0 98), the regional cerebral blood flow study didn't show significant difference between brain cortex area of lesioned side and its counterpart of the intact side (P0 05) Conclusions The 6 OH DA lesioned side DA content decreased significantly and thus induced a compensative up regulation of striatum D 2 receptor binding sites in hemi Parkinsonism rats, which show good correlation with rotation behavior induced by Apo Comparing with cerebral blood flow, D 2 receptor reflected by IBZM seems to be more specific and earlier to detect the cerebral functional impairment in experimental hemi Parkinsonism","PeriodicalId":221169,"journal":{"name":"Chinese Journal of Nuclear Mdeicine","volume":"26 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2000-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"133416000","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0