Chinese Journal of Nuclear Mdeicine最新文献

{"title":"An experimental study on cerebral ischemic penumbra imaging with ~(99)Tc~m-HL91","authors":"Shuliang Lu, Jun Xiang, C. Qing, Shu-wen Jin, Z. Liao","doi":"10.3760/CMA.J.ISSN.0253-9780.2002.03.022","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0253-9780.2002.03.022","url":null,"abstract":"Objective To investigate the biodistribution of 99 Tc m-4,9-diaza-3,3,10,10-tetramethyldodecan-2,11-dione dioxime (HL91) in rat model of middle cerebral artery occlusion (MCAO). Methods Thirty-one MCAO rats were established. Fourteen rats were used to study the biodistribution of 99 Tc m-HL91 and 15 rats were used to study the distribution of 99 Tc m-HL91 in the brain of MCAO model rats. Autoradiographic study of brain was also done in 16 MCAO model rats. Results The liver and kidney retention were higher than that in other tissues. At 1 h after injection, small intestine retention was also high.But radioactivity in normal brain was low. Retention in target site was higher than that in non-target site. Difference between subgroups of operation and that of pseudo-operation was significant( P 0.05 to P 0.001). Regional selective retention of 99 Tc m-HL91 at the target-ischemic area was shown in the autoradiograph. By using computer-enhanced image analysis, difference between target site and non-target site in the same autoradiograph and the differences between operation subgroups and that of pseudo-subgroups were all significant via Dunnett t -test and One-Way ANOVA. Conclusions 99 Tc m-HL91 can be avidly taken up by ischemic penumbra and target/non-target ratio is high. 99 Tc m-HL91 is a potential agent for hypoxic tissue imaging, and 99 Tc m-HL91 SPECT is a promising modality in detecting the ischemic penumbra.","PeriodicalId":221169,"journal":{"name":"Chinese Journal of Nuclear Mdeicine","volume":"133 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2002-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"123066081","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A study of the clinical factors correlate to glomerular filtration rate","authors":"Shuliang Lu, Jun Xiang, C. Qing, Shu-wen Jin, Z. Liao","doi":"10.3760/CMA.J.ISSN.0253-9780.2002.03.024","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0253-9780.2002.03.024","url":null,"abstract":"Objective To investigate the relative correlation between glomerular filtration rate(GFR) and various clinical factors and to offer theory basis for the measurement of GFR by the calculation method. Methods GFR of 50 cases of normal renal function group and 72 cases of renal insufficient group were determined using clearance of 99 Tc m-DTPA, and serum creatinine(SCr). Blood urea nitrogen, uric acid, serum potassium, sodium, calcium and hemoglobin were determined simultaneously; and heart rate, urine specific gravity, 24 h urine protein quantum, average artery pressure, body-weight index and body-surface area were determined simultaneously as well. Age and isotope dose for determining GFR were recorded. The simple correlation analysis of GFR in every group with different primary diseases to every other clinical factor above-mentioned were performed and the multiple correlation analysis of every clinical factor above-mentioned that were significantly correlated with GFR to GFR were performed again. Results For normal renal function group and renal insufficient group, correlation coefficient ( r ) between GFR and SCr were -0.449 and -0.275, respectively ( P0.05) . The correlations between GFR and other clinical factors were with different trends. Conclusion In renal insufficient patients with different primary diseases and normal renal function subjects the SCr is the ideal index for estimating GFR.","PeriodicalId":221169,"journal":{"name":"Chinese Journal of Nuclear Mdeicine","volume":"45 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2002-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"127380939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A comparative study on ~(67)Ga-Cit imaging and CT or MRI in diagnosis of maxillofacial neoplasms","authors":"Shuliang Lu, Jun Xiang, C. Qing, Shu-wen Jin, Z. Liao","doi":"10.3760/CMA.J.ISSN.0253-9780.2002.03.007","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0253-9780.2002.03.007","url":null,"abstract":"Objective To evaluate the clinical value of 67 Ga-Citrate(Cit) imaging and CT or MRI in diagnosis of maxillofacial neoplasms. Methods Before surgery, 71 patients with maxillofacial neoplasms were studied with 67 Ga-Cit imaging and CT or MRI. All results were compared with pathological findings. Results ①In 36 patients with benign maxillofacial mass, 28 cases were with negative 67 Ga-Cit imaging (77.8%, 28/36), whereas 8 cases with positive results (22.2%, 8/36); in 35 patients with malignant tumors, 33 cases with positive 67 Ga-Cit imaging (94.3%, 33/35), whereas 2 cases with negative results (5.7%, 2/35). ②For CT or MRI, in 24 patients with benign maxillofacial mass, only 4 cases were found in concordance with pathological findings (16.7%, 4/24), but in 24 patients with malignant maxillofacial tumors, 6 cases were found in concordance with pathological findings(25.0%, 6/24). In the differentiating malignant maxillofacial tumors from benign ones, there was significant difference in accuracy between these modalities ( χ 2=21.60, 19.91, both were P 0.01). For the assessment of tumor size, there was no difference among the three modalities ( χ 2=1.28, 0.26 and 1.76, respectively, all were P 0.05). Conclusion The accuracy of 67 Ga-Cit imaging in differentiating malignant maxillofacial tumors from benign ones is significantly higher than that of CT or MRI.","PeriodicalId":221169,"journal":{"name":"Chinese Journal of Nuclear Mdeicine","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2002-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132282526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A study of labeling of monoclonal antibody C50 with ~(99)Tc~m and using for radioimmunoimaging","authors":"Shuliang Lu, Jun Xiang, C. Qing, Shu-wen Jin, Z. Liao","doi":"10.3760/CMA.J.ISSN.0253-9780.2002.03.019","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0253-9780.2002.03.019","url":null,"abstract":"Objective To provide an accurate and scientific method of radionuclide imaging with 99 Tc m-C50. Methods The labeled C50 was derived by iminothiolane and ligand exchange from 99 Tc mO(GH) -_2. HPLC was used to analyse the modified antibody and labeled antibody, labeling efficiency was measured by paper chromatography. Radioimmunoimaging was performed on human colon tumor bearing nude mice. Results The labeling efficiency of the labeled antibody was 97%, with 4% colloidal 99 Tc m in it; the in vitro competition test showed that 99 Tc m being bound up with the antibody at high affinity. The stability of the iminothiolane modified antibody could be kept at 4 ℃ for 3 months. The biodistribution study showed that the tumor radioactivity uptake at 24 h postinjection was the highest, the ratio of tumor to muscle was 4.03. Conclusion The labeling method applied to the preparation of 99 Tc m labeled antibody C50 was successful, and appears to be equally applicable for labeling other antibodies.","PeriodicalId":221169,"journal":{"name":"Chinese Journal of Nuclear Mdeicine","volume":"40 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2002-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115188415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The relationship between ~(99)Tc~m-MIBI uptake and cell death or proliferative activity after irradiation of tumor","authors":"Shuliang Lu, Jun Xiang, C. Qing, Shu-wen Jin, Z. Liao","doi":"10.3760/CMA.J.ISSN.0253-9780.2002.03.009","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0253-9780.2002.03.009","url":null,"abstract":"Objective To evaluate the potential of 99 Tc m-MIBI imaging for monitoring cellular viability of tumor post-irradiation. Methods One hundred and five female Kunming mice bearing Ehrlich carcinoma in the left thigh were exposed to 60 Co at a single dose of 0, 5, 10, 15 and 20 Gy, respectively, and then the following protocols were performed at 6 h pre-irradiation and 24, 72, 144 h post-irradiation, respectively (every group with different irradiation dose or observation time consisted of six to eight mice): ① 99 Tc m-MIBI planer scintigraphy was performed, then the tumor sample was excised and its radioactivity was counted in a γ counter. Differential uptake ratio (DUR) and tumor-to- nontarget (T/NT) ratio were used to quantitate the MIBI uptake. ②Single-cell suspension of tumor was prepared and apoptosis index (AI) was measured by flow cytometry; meanwhile, paraffin sections were stained with HE or proliferating cell nuclear antigen (PCNA) immunohistochemistrily, then percentages of necrosis area (PNA) and PCNA integral absorbance (PCNA-IA) were measured with a HPIAS-1000 imaging analysis system. Results ①Compared with the group with 0 Gy, the DUR of the groups with 10, 15, 20 Gy decreased markedly starting from 24 h post-irradiation and the decrease of DUR in 5 Gy group was much milder. Within the groups with similar irradiation dose, DUR kept decreasing with the time past irradiation. At the same post-irradiation time point, DUR decreased with dose escalating. The change of T/NT was similar to DUR. There was a perfect linear correlation between DUR and T/NT ( n=105, r=0.976, P 0.01). ②Compared with the group with 0 Gy, at 24 h post-irradiation, the AI and PNA in other groups increased but PCNA-IA decreased with dose escalating. However, at 72 and 144 h post-irradiation, AI reversed to the same level as in 0 Gy group and PCNA-IA rose close to the level of 0 Gy group, but PNA increased continuously. At the same time point, PNA increased with dose escalating. ③At 24 h post-irradiation, DUR or T/NT was negatively correlated with AI or PNA ( n=33, r =-0.849, -0.829; -0.883, -0.855, respectively, P 0.01) and positively correlated with PCNA-IA ( n=33, r= 0.789, 0.742, respectively, P 0.01). At 72 and 144 h post-irradiation, DUR or T/NT was only negatively correlated with PNA ( n=33, r= -0.967, -0.956; -0.915, -0.886, respectively, P 0.01). Conclusions 99 Tc m-MIBI uptake of tumor cells correlated with the changes of cell viability after irradiation. 99 Tc m-MIBI imaging may be helpful to monitoring tumor cell viability or therapeutic response after irradiation.","PeriodicalId":221169,"journal":{"name":"Chinese Journal of Nuclear Mdeicine","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2002-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"116257552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The study on mutations of the gene of extracellular domain of human thyrotropin receptor in the patients with thyroid diseases","authors":"Shuliang Lu, Jun Xiang, C. Qing, Shu-wen Jin, Z. Liao","doi":"10.3760/CMA.J.ISSN.0253-9780.2002.03.021","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0253-9780.2002.03.021","url":null,"abstract":"Objective To define the sequence of the gene of extracellular domain of normal human thyrotropin receptor (hTSHR) and to investigate into the mutations of the gene in the patients with thyroid diseases. Methods Total RNAs were extracted from the thyroid tissue of four normal controls, twelve Graves' disease, four Hashimoto's thyroiditis and eleven nodular goiter patients. The extracellular domain of hTSHR genes were amplified by reverse transcription-polymerase chain reaction (RT-PCR) and sequenced with CEQ 2000 Genetic Analyzer. Results The normal controls and the patients with thyroid disease had the same gene sequences of the extracellular domain of hTSHR. No mutation was found, except a silent base exchange in exon 7 (Asn187) at 661 base, in which 20 samples were \"T\", 11 samples were \"C\", without changes of amino acid of the TSHR. Conclusions This study has not revealed mutations in the gene of extracellular domain of hTSHR. Other molecular pathogenetic mechanisms may be involved and more research is demanded.","PeriodicalId":221169,"journal":{"name":"Chinese Journal of Nuclear Mdeicine","volume":"23 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2002-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"125777636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Image registration and fusion via picture archiving and communication system","authors":"Shuliang Lu, Jun Xiang, C. Qing, Shu-wen Jin, Z. Liao","doi":"10.3760/CMA.J.ISSN.0253-9780.2002.03.017","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0253-9780.2002.03.017","url":null,"abstract":"Objective The preliminary studies of the multimodality image registration and fusion were performed using picture archiving and communication system (PACS) and image fusion software to explore the methodology. Methods The original image volume data were acquired with Siemens Somatom Plus S CT scanner, Magneton Vision 1.5T MR and E.CAM + dual-head coincidence SPECT, respectively. The data sets from all imaging devices were acquired, retrieved, transferred and accessed via DICOM PACS. The image fusion was performed at SPECT ICON work-station, where the medical image merge (MIM)fusion software was installed. The images were created by reslicing original volume on the fly. The image volumes were aligned by translation and rotation of these view ports with respect to the original volume orientation. The transparency factor and contrast were adjusted in order that both volumes can be visualized in the merged images. Results The image volume data of CT, MR and nuclear medicine were transferred, accessed and loaded via PACS successfully. The perfectly fused images of brain MR/SPECT and chest CT/ 18 F-FDG were obtained. Conclusions The results showed that the image fusion technique using PACS was feasible and practical. Further experimentation and larger validation studies are needed to explore the full potential of the clinical use.","PeriodicalId":221169,"journal":{"name":"Chinese Journal of Nuclear Mdeicine","volume":"16 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2002-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"132520101","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The preliminary study of the blood perfusion and ammonia metabolism of pituitary using dynamic ~(13)N-NH_3 PET imaging","authors":"Shuliang Lu, Jun Xiang, C. Qing, Shu-wen Jin, Z. Liao","doi":"10.3760/CMA.J.ISSN.0253-9780.2002.03.004","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0253-9780.2002.03.004","url":null,"abstract":"Objective To preliminarily study the blood perfusion and ammonia metabolism of pituitary using dynamic 13 N-NH_3 PET imaging. Methods 13 N-NH_3 PET imaging was performed on 21 subjects without pituitary diseases, 6 of them underwent dynamic PET imaging, and 8 of them underwent brain MRI in addition to PET. PET images were registered with MRI. Results The pituitary could be clearly seen in 13 N-NH_3 PET images, and being confirmed by PET/MRI image fusion. The size of pituitary was (1.07±0.17) cm×(1.09±0.15) cm×(1.14±0.17) cm, the standard uptake value (SUV) was 3.84±1.75, and the radioactivity ratio of pituitary to thalamus was 1.35±0.63. Pituitary image was seen at 10 s after the internal carotid was seen in dynamic 13 N-NH_3 PET imaging. 13 N-NH_3 was retained in pituitary, and was hardly cleaned out within 20 min. The radioactivity ratio of pituitary to internal carotid was 0.75±0.13 when the radioactivity of internal carotid was at its highest level. Conclusions The blood flow and ammonia metabolism of pituitary can be observed with dynamic 13 N-NH_3 PET imaging. Ammonia is highly extracted by pituitary, and metabolized in pituitary cells.","PeriodicalId":221169,"journal":{"name":"Chinese Journal of Nuclear Mdeicine","volume":"88 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2002-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115886306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of the association constant and the maximum binding capacity of radionuclide labelled monoclonal antibody","authors":"Chen Wang, Xian-sheng Cheng","doi":"10.3760/CMA.J.ISSN.0253-9780.2001.05.022","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0253-9780.2001.05.022","url":null,"abstract":"Objective To determine the maximum number of McAb molecules bound by per antigen cell and the association constant of the binding reaction. Methods Using binding assay for binding of radionuclide labelled McAb with target cell antigen, the formula of equilibrium plot was T/B=1+1/ K a·C+T/C (1) and F/B=1/ K a·C+F/C (2), K a,T,B,F and C were the association constants, the concentration of total, binding, free McAb and constant cell antigen volumn, respectively. The experimental data demonstrated a very significant linear correlationship of formula(1) T/B to T and formula (2) F/B to F and that was calculated by means of linear regression analysis. Results The inverse T/B value of intersecting point of formula (1) straight line with the T/B axis was the immunoreactive fraction of labelled McAb. Using constant labelled McAb concentration and different unlabelled McAb substitute for T, the distance from the intersecting point of this line with T/B value of immunoreactive fraction to T/B axis was the actual concentration of immunoreactive fraction of labelled McAb. The maximum number of McAb molecules bound by per antigen cell was the inverse of the slope of formula (2)straight line and the association constant was the slope divided by intercept of formula (2) straight line with F/B axis. Conclusion The method is feasible for determination of the association constant and the maximum number of McAb molecules bound by per antigen cell.","PeriodicalId":221169,"journal":{"name":"Chinese Journal of Nuclear Mdeicine","volume":"73 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2001-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"115923971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"~(67)Ga scinitigraphy monitioring of therapy in Non-Hodgkin's lymphoma","authors":"Chen Wang, Xian-sheng Cheng","doi":"10.3760/CMA.J.ISSN.0253-9780.2001.05.020","DOIUrl":"https://doi.org/10.3760/CMA.J.ISSN.0253-9780.2001.05.020","url":null,"abstract":"Objective In early phase of therapy in Non Hodgkin's lymphoma (NHL), 67 Ga imaging was used to predict and monitor the therapeutic response. Methods In 86 patients with NHL proven by clinical syndrome and pathology and with positive 67 Ga imaging and CT scan before therapy, 67 Ga scintigraphy was performed after 2 and 4 cycles of therapy.CT scan was also performed after 4 cycles of therapy on 51 of the 86 patients.Average following time was 1.5 years (3～30 months).The therapeutic results were classified as:①complete remission (CR):the lesion uptake of 67 Ga was not detected;②partial remission (PR):the lesion uptake of 67 Ga was 50% less than that before therapy;③no remission (NR):the lesion uptake of 67 Ga was the same as or more than that before therapy. Results Based on 67 Ga imaging in 86 patients,61 patients were in CR after therapy,14 were in PR,and 11 were in NR.During the follow up,the negative predictive value (NPV) after 2 cycles of therapy was 90% (43/48),but negative predictive value after 4 cycles of therapy was 69% (9/13),suggesting that early NPV has more important significance.In PR and NR,NPV was only 36% (9/35).CT findings:in 51 patients undergone CT scan after therapy,18 were negative and 33 were positive.During the follow up,the NPV was 67% (12/18),the positive predictive value was only 27% (9/33).Statastical results showed that the 67 Ga imaging could effectively predict the therapeutic response ( P 0.001),but the CT could not ( P 0.05). Conclusions The early 67 Ga imaging after therapy may help confirm the presence of active NHL better than late 67 Ga imaging does;patients with PR or NR may have poor prognosis; 67 Ga imaging is better than CT in prediction and evaluation of therapeutic outcome in NHL.","PeriodicalId":221169,"journal":{"name":"Chinese Journal of Nuclear Mdeicine","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2001-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"130576156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}