ACS Nano最新文献_第7页

Spatial and Temporal Single-Cell Profiling of RNA Compartmentalization in Neurons with Nanotweezers 纳米镊子对神经元中RNA区隔化的时空单细胞分析

IF 17.1 1区材料科学

ACS Nano Pub Date : 2025-05-06 DOI: 10.1021/acsnano.5c02056

Annie Sahota, Binoy Paulose Nadappuram, Zoe Kwan, Flavie Lesept, Jack H. Howden, Suzanne Claxton, Josef T. Kittler, Michael J. Devine, Joshua B. Edel, Aleksandar P. Ivanov

{"title":"Spatial and Temporal Single-Cell Profiling of RNA Compartmentalization in Neurons with Nanotweezers","authors":"Annie Sahota, Binoy Paulose Nadappuram, Zoe Kwan, Flavie Lesept, Jack H. Howden, Suzanne Claxton, Josef T. Kittler, Michael J. Devine, Joshua B. Edel, Aleksandar P. Ivanov","doi":"10.1021/acsnano.5c02056","DOIUrl":"https://doi.org/10.1021/acsnano.5c02056","url":null,"abstract":"Emerging techniques for mapping mRNAs within the subcellular compartments of live cells hold great promise for advancing our understanding of the spatial distribution of transcripts and enabling the study of single-cell dynamics in health and disease. This is particularly critical for polarized cells, such as neurons, where mRNA compartmentalization is essential for regulating gene expression, and defects in these localization mechanisms are linked to numerous neurological disorders. However, many subcellular analysis techniques require a compromise between subcellular precision, live-cell measurements, and nondestructive access to single cells in their native microenvironment. To overcome these challenges, we employ a single-cell technology that we have recently developed, the nanotweezer, which features a nanoscale footprint (∼100 nm), avoids cytoplasmic fluid aspiration, and enables rapid RNA isolation from living cells with minimal invasiveness. Using this tool, we investigate single-cell mRNA compartmentalization in the soma and dendrites of hippocampal neurons at different stages of neuronal development. By combining precise targeting with sequential sampling, we track changes in mRNA abundance at dendritic spine regions of the same neuron, both before and after stimulation. This minimally invasive approach enables time-resolved, subcellular gene expression profiling of the same single cell. This could provide critical insights into polarized cells and advance our understanding of biological processes and complex diseases.","PeriodicalId":21,"journal":{"name":"ACS Nano","volume":"31 1","pages":""},"PeriodicalIF":17.1,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143910779","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Hierarchical Contact–Electrification Interface Based on Gradient Micro-/Nanostructured Hydrogel for Cardiovascular Disease Monitoring 基于梯度微/纳米结构水凝胶的分层接触-电气化界面用于心血管疾病监测

IF 17.1 1区材料科学

ACS Nano Pub Date : 2025-05-05 DOI: 10.1021/acsnano.5c00313

Zhenqiu Gao, Liming Zhang, Hao Lei, Yina Liu, Haicheng Gu, Lingjie Xie, Bohan Lu, Haifeng Ji, Zhen Wen, Xuhui Sun

{"title":"A Hierarchical Contact–Electrification Interface Based on Gradient Micro-/Nanostructured Hydrogel for Cardiovascular Disease Monitoring","authors":"Zhenqiu Gao, Liming Zhang, Hao Lei, Yina Liu, Haicheng Gu, Lingjie Xie, Bohan Lu, Haifeng Ji, Zhen Wen, Xuhui Sun","doi":"10.1021/acsnano.5c00313","DOIUrl":"https://doi.org/10.1021/acsnano.5c00313","url":null,"abstract":"Accurate monitoring of pulses is essential for assessing cardiovascular health. However, the specificity of the pulse wave depends on prestress applied to a wearable sensor. Here, we introduce a progressive contact area compensation strategy, which greatly extends the detection range of the sensor’s high-sensitivity region. It features a hierarchical flower surface structure and a gradient micro-/nanostructured hydrogel as the dielectric layer, compensating for the output decrease resulting from pressure hardening by gradually increasing the contact area between the contact–electrification interfaces. Consequently, the gradient micro-/nanostructured hydrogel, fabricated via electric field induction, enables the sensor’s high-sensitivity region to reach 1.1–52.2 kPa, a 5-fold improvement over that of comparable sensors. By integrating prestress adaptive units, signal processing modules, and a peak seeking algorithm, we develop a wireless wristband for continuous monitoring of cardiovascular status and blood pressure. Importantly, a preliminary 10 day blood pressure test on 22 volunteers showed an error margin of less than ±5 mm Hg, demonstrating its potential as a cardiovascular health product.","PeriodicalId":21,"journal":{"name":"ACS Nano","volume":"76 1","pages":""},"PeriodicalIF":17.1,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143905651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High-Efficiency Electrochemical Ammonia Synthesis at Co-Catalytic Fe–Mo Dual-Atom Sites Fe-Mo双原子催化下的高效电化学氨合成

IF 17.1 1区材料科学

ACS Nano Pub Date : 2025-05-05 DOI: 10.1021/acsnano.5c01741

Ruonan Li, Runlin Ma, Li-Li Zhang, Wei Ma, Gonglei Shao, Xu Zhang, Yun Tian, Menggai Jiao, Zhen Zhou

{"title":"High-Efficiency Electrochemical Ammonia Synthesis at Co-Catalytic Fe–Mo Dual-Atom Sites","authors":"Ruonan Li, Runlin Ma, Li-Li Zhang, Wei Ma, Gonglei Shao, Xu Zhang, Yun Tian, Menggai Jiao, Zhen Zhou","doi":"10.1021/acsnano.5c01741","DOIUrl":"https://doi.org/10.1021/acsnano.5c01741","url":null,"abstract":"To tackle the challenge in electrochemical nitrogen fixing and reduction in aqueous electrolytes, the conventional approach has been to suppress the competitive hydrogen evolution reaction. Nonetheless, proton provision is a crucial step in the nitrogen reduction pathway to produce ammonia, and a single active site faces the daunting task in striking a balance between high nitrogen fixation efficiency and fast protonation kinetics. This work presents a harmonic strategy featuring atomically dispersed dual Fe–Mo sites anchored in an N-doped carbon (FeMoNC) substrate, where a low-spin Fe center with enriched empty d orbitals aids in nitrogen fixation and activation, and the adjacent Mo site accelerates the protonation kinetics of N-containing intermediates at the Fe site via a distal associative mechanism. Driven by this co-catalytic mechanism, the FeMoNC catalyst achieves a Faradaic efficiency of 37.42%, marking a significant improvement of 7.8- and 10.6-fold over Fe or Mo single-atom catalysts, respectively. Furthermore, an excellent NH3 yield of 54.40 μg h–1 mgcat.–1 is realized in a flow cell by enhancing mass transfer. This study provides valuable insights into diatomic co-catalytic mechanisms for electrochemical ammonia synthesis.","PeriodicalId":21,"journal":{"name":"ACS Nano","volume":"93 1","pages":""},"PeriodicalIF":17.1,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143905646","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Remodeling the Senescent Microenvironment for Promoting Osteoporotic Tendon-to-Bone Healing via Synergizing Senolytic Quercetin and Aligned Nanowire-Structured Hydrogels 通过协同抗衰老槲皮素和排列纳米线结构水凝胶，重塑衰老微环境促进骨质疏松肌腱到骨愈合

IF 17.1 1区材料科学

ACS Nano Pub Date : 2025-05-05 DOI: 10.1021/acsnano.5c01332

Wei Song, Wencai Liu, Si-Yi Li, Yuhao Yu, Hui Xu, Tingwang Shi, Han-Ping Yu, Yaohua He, Ying-Jie Zhu, Weilin Yu

{"title":"Remodeling the Senescent Microenvironment for Promoting Osteoporotic Tendon-to-Bone Healing via Synergizing Senolytic Quercetin and Aligned Nanowire-Structured Hydrogels","authors":"Wei Song, Wencai Liu, Si-Yi Li, Yuhao Yu, Hui Xu, Tingwang Shi, Han-Ping Yu, Yaohua He, Ying-Jie Zhu, Weilin Yu","doi":"10.1021/acsnano.5c01332","DOIUrl":"https://doi.org/10.1021/acsnano.5c01332","url":null,"abstract":"Osteoporotic tendon-to-bone healing remains a major challenge, as cellular senescence disrupts tissue regeneration and impairs repair outcomes. Although the role of cellular senescence in rotator cuff repair is increasingly recognized, current strategies often overlook the complex pathological context, particularly the dual impacts of senescence on both bone marrow-derived mesenchymal stem cells (BMSCs) and tendon-derived stem cells (TDSCs). This gap hampers effective tendon-to-bone healing and integration, especially under osteoporotic conditions. Herein, a composite hydrogel system, quercetin-loaded aligned ultralong hydroxyapatite nanowire/gelatin-hyaluronic acid hydrogel (Que-AHNW/GH), has been developed to address these challenges. By integrating senolytic quercetin as a biological cue with highly aligned ultralong hydroxyapatite (HAP) nanowires as a topographical cue, the system remodels the senescent microenvironment, alleviating senescence in both BMSCs and TDSCs and promoting osteogenesis and tenogenesis. Que-AHNW/GH suppresses the PI3K/AKT pathway, enhances autophagy, and reduces senescence in both cell types. In vivo, Que-AHNW/GH improves bone tunnel regeneration, tendon repair, and tendon-to-bone integration in osteoporotic rats with rotator cuff injury. This system enhances biomechanical strength and gait performance and demonstrates excellent biosafety. These findings highlight the promising potential of Que-AHNW/GH as a multifunctional biomaterial for effectively promoting senescence-related tendon-to-bone healing, offering a promising solution for treating osteoporotic tendon-to-bone injuries.","PeriodicalId":21,"journal":{"name":"ACS Nano","volume":"31 1","pages":""},"PeriodicalIF":17.1,"publicationDate":"2025-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143910729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to “Tumor Microenvironment-Activatable Nanosystem Capable of Overcoming Multiple Therapeutic Obstacles for Augmenting Immuno/Metal-Ion Therapy” 修正“肿瘤微环境可激活纳米系统能够克服增强免疫/金属离子治疗的多重治疗障碍”

IF 17.1 1区材料科学

ACS Nano Pub Date : 2025-05-05 DOI: 10.1021/acsnano.5c06637

Qian Cheng, Xiaolei Shi, Yuzhe Chen, Qilin Li, Jiawei Wang, Heli Li, Lin Wang, Zheng Wang

引用次数: 0

Self-Assembly of Nanogold Triplets on Trimeric Viral Proteins for Infectious Disease Diagnosis 纳米金三胞胎在三聚体病毒蛋白上的自组装用于传染病诊断

IF 17.1 1区材料科学

ACS Nano Pub Date : 2025-05-05 DOI: 10.1021/acsnano.4c17685

Wenpeng Liu, Ahmed Soliman, Hagar E. Emam, Jun Zhang, Joseph V. Bonventre, Luke P. Lee, Mahmoud L. Nasr

引用次数: 0

Ultrafast Thermal Engineering in Energy Materials: Design, Recycling, and Future Directions 能源材料中的超快热工：设计、回收和未来方向

IF 17.1 1区材料科学

ACS Nano Pub Date : 2025-05-04 DOI: 10.1021/acsnano.5c04768

Pandeng Zhao, Xingqiao Wu, Yinghao Zhang, Wenjie Huang, Yuhai Dou, Hua Kun Liu, Shixue Dou, Minghong Wu, Shulei Chou

{"title":"Ultrafast Thermal Engineering in Energy Materials: Design, Recycling, and Future Directions","authors":"Pandeng Zhao, Xingqiao Wu, Yinghao Zhang, Wenjie Huang, Yuhai Dou, Hua Kun Liu, Shixue Dou, Minghong Wu, Shulei Chou","doi":"10.1021/acsnano.5c04768","DOIUrl":"https://doi.org/10.1021/acsnano.5c04768","url":null,"abstract":"Energy materials are essential for addressing global energy challenges, and their design, recycling, and performance optimization are critical for sustainable development. To efficiently rise to this occasion, advanced technology should be explored to address these challenges. This review focuses on the potential of ultrafast thermal engineering as an innovative approach to the design and recycling of energy materials and systematically examines ultrahigh temperature shock’s origins, mechanisms, and developmental progress, clarifying fundamental differences between the Joule heating and carbothermal shock modes. Recent advancements in lithium/sodium battery electrode fabrication, catalyst synthesis, and battery recycling by this technology are comprehensively summarized to highlight the processing parameters, structural modulation mechanisms, and underlying principles. The review also explores the mechanisms of ultrahigh temperature shock processes, their scalability, and their environmental and economic implications. Notably, a mechanistic insight into the dynamic coexistence of Joule heating and carbothermal shock in UTS is proposed, which may synergistically govern structural evolution in poor conductivity/insulating materials. This review ultimately aims to drive the development and application of ultrafast thermal engineering in the energy materials field.","PeriodicalId":21,"journal":{"name":"ACS Nano","volume":"60 1","pages":""},"PeriodicalIF":17.1,"publicationDate":"2025-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143905444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Agarose Microgel-Based In Situ Cleavable Immuno-Rolling Circle Amplification for Multiplexed Single-Molecule Quantitation on Single Extracellular Vesicles 基于琼脂糖微凝胶的原位可切割免疫滚圈扩增用于单个细胞外囊泡的多重单分子定量

IF 17.1 1区材料科学

ACS Nano Pub Date : 2025-05-04 DOI: 10.1021/acsnano.5c04207

Juhwan Park, Michelle Feng, Jingbo Yang, Hanfei Shen, Zhiyuan Qin, Wei Guo, David A. Issadore

{"title":"Agarose Microgel-Based In Situ Cleavable Immuno-Rolling Circle Amplification for Multiplexed Single-Molecule Quantitation on Single Extracellular Vesicles","authors":"Juhwan Park, Michelle Feng, Jingbo Yang, Hanfei Shen, Zhiyuan Qin, Wei Guo, David A. Issadore","doi":"10.1021/acsnano.5c04207","DOIUrl":"https://doi.org/10.1021/acsnano.5c04207","url":null,"abstract":"We have developed a platform for the multiplexed and ultrasensitive profiling of individual extracellular vesicles (EVs) directly in plasma, which we call GDEVA─Agarose microGel-based Digital single-molecule–single EV Assay. GDEVA achieves single-molecule sensitivity and moderate multiplexing (demonstrated 3-plex), and can achieve a throughput of ∼104 EVs per minute necessary to resolve EVs directly in human plasma when read out using flow cytometry. Our platform integrates a rolling circle amplification (RCA) immunoassay of EV surface proteins, which are cleaved from single EVs, and amplified within agarose microgels, followed by flow cytometry-based readout or imaging after fluorescence-activated cell sorting (FACS). It overcomes steric hindrance of RCA products, nonspecific binding of RCA templates, and the lack of quantitation of multiple proteins on EVs that have plagued earlier approaches. We evaluated the analytical capabilities of GDEVA through head-to-head comparison with conventional technology and demonstrated a ∼100× improvement in the limit of detection (LOD) of EV subpopulations. We evaluate GDEVA’s potential in cancer immunology, by analyzing single EVs in plasma samples from patients with melanoma, where EV heterogeneity plays a critical role in disease progression and response to therapy. We demonstrate profiling of individual EVs for key immune markers PD-L1, CD155, and the melanoma marker TYRP-1, and showed that GDEVA can precisely quantify EVs, offering the resolution to detect rare EV subpopulations in complex clinical specimens.","PeriodicalId":21,"journal":{"name":"ACS Nano","volume":"34 1","pages":""},"PeriodicalIF":17.1,"publicationDate":"2025-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143905650","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of Surface Bands in the Photogeneration, Cooling, and Recombination of Charge Carriers in Two-Dimensional Bi2Se3 表面带在二维Bi2Se3载流子的光生、冷却和重组中的作用

IF 17.1 1区材料科学

ACS Nano Pub Date : 2025-05-04 DOI: 10.1021/acsnano.4c14134

Jara F. Vliem, Servet Ataberk Cayan, Riccardo Reho, Andrés R. Botello-Méndez, Pieter Geiregat, Zeila Zanolli, Daniel Vanmaekelbergh

{"title":"Role of Surface Bands in the Photogeneration, Cooling, and Recombination of Charge Carriers in Two-Dimensional Bi2Se3","authors":"Jara F. Vliem, Servet Ataberk Cayan, Riccardo Reho, Andrés R. Botello-Méndez, Pieter Geiregat, Zeila Zanolli, Daniel Vanmaekelbergh","doi":"10.1021/acsnano.4c14134","DOIUrl":"https://doi.org/10.1021/acsnano.4c14134","url":null,"abstract":"Bi2Se3, a layered three-dimensional topological insulator, exhibits intriguing changes in its band structure when its thickness is reduced below 7 quintuple layers. The reduction in thickness leads to hybridization between the surface states and the opening of a gap between these states. We combine density functional theory calculations with pump–probe spectroscopy to explore how these hybridized states affect the photogeneration, cooling, and recombination of charge carriers in two-dimensional Bi2Se3 nanoplatelets. Our calculations reveal that the hybridized surface states are crucial for understanding the optical transitions. By comparing the experimental absorption spectrum with the calculated absorptance in the near-infrared-visible region, we identify key transitions within the 2D Brillouin zone. We distinguish transitions involving the hybridized surface states from those involving the interior layers. We observe a significant delay of several picoseconds in carrier recombination when surface state transitions are excited, which we attribute to carrier accumulation in the valleys of the Rashba-shaped surface-state valence band and in higher-lying surface states of the conduction band. These findings emphasize the important role of surface state bands in the optical behavior of Bi2Se3 and their potential for manipulating carrier dynamics in two-dimensional materials.","PeriodicalId":21,"journal":{"name":"ACS Nano","volume":"18 1","pages":""},"PeriodicalIF":17.1,"publicationDate":"2025-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143905679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ultrahigh Capacitive Energy Storage in Lead-Free Relaxors via Localizing Distortion 基于局域畸变的无铅弛豫器超高电容储能

IF 17.1 1区材料科学

ACS Nano Pub Date : 2025-05-04 DOI: 10.1021/acsnano.5c02357

Liang Chen, Yuming Zhang, He Qi, Ruiyu Wang, Yunxuan Ji, Takumi Nishikubo, Masaki Azuma, Chang Zhou, Jun Chen

{"title":"Ultrahigh Capacitive Energy Storage in Lead-Free Relaxors via Localizing Distortion","authors":"Liang Chen, Yuming Zhang, He Qi, Ruiyu Wang, Yunxuan Ji, Takumi Nishikubo, Masaki Azuma, Chang Zhou, Jun Chen","doi":"10.1021/acsnano.5c02357","DOIUrl":"https://doi.org/10.1021/acsnano.5c02357","url":null,"abstract":"The significant polarization hysteresis under external fields can be stimulated by long-range ordered distortions, including cation displacements and oxygen octahedral tilts, deteriorating the energy storage performance and reliable operation of dielectric capacitors. Here, we propose a strategy of localized distortion to craft a disordered nanostructure landscape, manifested as strongly polar orthorhombic rocks dissociated in the transition region of polymorphic nanoclusters and strong oxygen-tilted blocks embedded in the weak oxygen distortion region, resulting in a smooth polarization response trajectory with large polarization fluctuations, small hysteresis, and delayed polarization saturation. Through localizing distortion, an ultrahigh recoverable energy density of 12.5 J cm–3 can be realized with an inspiring efficiency of 87%, alongside ultrawide capacitance temperature stability (from −100 to 432 °C) far exceeding X9R criteria, showing breakthrough progress in the overall performance for NaNbO3-based lead-free bulk ceramics. This work unveils an effective avenue of localized distortion to develop dielectrics with excellent energy storage performance and the potential to be extended to other functionalities.","PeriodicalId":21,"journal":{"name":"ACS Nano","volume":"113 1","pages":""},"PeriodicalIF":17.1,"publicationDate":"2025-05-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143905649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":1,"RegionCategory":"材料科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0