纳米金三胞胎在三聚体病毒蛋白上的自组装用于传染病诊断

IF 15.8 1区材料科学 Q1 CHEMISTRY, MULTIDISCIPLINARY

ACS Nano Pub Date : 2025-05-05 DOI:10.1021/acsnano.4c17685

Wenpeng Liu, Ahmed Soliman, Hagar E. Emam, Jun Zhang, Joseph V. Bonventre, Luke P. Lee, Mahmoud L. Nasr

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引用次数: 0

摘要

对传染病进行及时和准确的诊断对于防止其在全球传播至关重要。尽管快速诊断测试因其速度、成本效益和易用性而受到青睐，但大多数测试都会降低灵敏度，从而有可能产生假阴性结果。在这里，我们提出了纳米金三联体在三聚体病毒表面蛋白上的自组装，用于灵敏的比色测定来识别病毒。利用超小金纳米颗粒在病毒三聚体表面蛋白上自组装金三联体。我们观察到70 nm的显著波长位移，使得通过金三重体作为产生纳米等离子体病毒的催化剂的直接裸眼检测成为可能。建立了有效比色法检测SARS-CoV-2的检出限为3 × 105 copies/ml。金三联体在三聚体病毒表面蛋白上的自组装为准确灵敏地检测病毒提供了一种可靠的方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Self-Assembly of Nanogold Triplets on Trimeric Viral Proteins for Infectious Disease Diagnosis

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Self-Assembly of Nanogold Triplets on Trimeric Viral Proteins for Infectious Disease Diagnosis

Timely and accurate diagnostics for infectious diseases are essential in preventing their worldwide spread. Though rapid diagnostic tests are favored for their speed, cost-effectiveness, and ease of use, most tests compromise sensitivity, which risks false-negative results. Here, we present the self-assembly of nanogold triplets on trimeric viral surface proteins for a sensitive colorimetric assay to identify viruses. Gold triplets were self-assembled on the viral trimeric surface proteins using ultrasmall gold nanoparticles. We observed a significant wavelength shift of 70 nm, enabling straightforward naked-eye detection through gold triplets that act as catalysts for producing nanoplasmonic viruses. We established the detection limit of 3 × 10⁵ copies/ml using an effective colorimetric assay for detecting SARS-CoV-2. The self-assembly of gold triplets on trimeric viral surface proteins provides a reliable approach to the accurate and sensitive detection of viruses.

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来源期刊

ACS Nano 工程技术-材料科学：综合

CiteScore

26.00

自引率

4.10%

发文量

1627

审稿时长

1.7 months

期刊介绍： ACS Nano, published monthly, serves as an international forum for comprehensive articles on nanoscience and nanotechnology research at the intersections of chemistry, biology, materials science, physics, and engineering. The journal fosters communication among scientists in these communities, facilitating collaboration, new research opportunities, and advancements through discoveries. ACS Nano covers synthesis, assembly, characterization, theory, and simulation of nanostructures, nanobiotechnology, nanofabrication, methods and tools for nanoscience and nanotechnology, and self- and directed-assembly. Alongside original research articles, it offers thorough reviews, perspectives on cutting-edge research, and discussions envisioning the future of nanoscience and nanotechnology.