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Establishment of the integration-free Class I and Class II 11 loci homozygous HLA iPSC line (MUSIi023-A) from peripheral blood mononuclear cells of a Thai donor 从泰国供者外周血单个核细胞中建立无整合ⅰ类和ⅱ类11位点纯合HLA iPSC系(MUSIi023-A)。
IF 0.7 4区 医学
Stem cell research Pub Date : 2025-10-01 DOI: 10.1016/j.scr.2025.103840
Nittaya Jiamvoraphong , Pattaraporn Nimsamer , Thidathip Wongsurawat , Chanchao Lorthongpanich , Chuti Laowtammathron , Surapol Issaragrisil
{"title":"Establishment of the integration-free Class I and Class II 11 loci homozygous HLA iPSC line (MUSIi023-A) from peripheral blood mononuclear cells of a Thai donor","authors":"Nittaya Jiamvoraphong ,&nbsp;Pattaraporn Nimsamer ,&nbsp;Thidathip Wongsurawat ,&nbsp;Chanchao Lorthongpanich ,&nbsp;Chuti Laowtammathron ,&nbsp;Surapol Issaragrisil","doi":"10.1016/j.scr.2025.103840","DOIUrl":"10.1016/j.scr.2025.103840","url":null,"abstract":"<div><div>Pluripotent stem cell (PSC)-based technologies offer a promising new approach to generating functional cells or grafts for transplantation. To minimize the risk of immune rejection, induced pluripotent stem cells (iPSCs) that are homozygous for HLA Class I and Class II genes are a potential solution to reduce the risk of immune rejection in regenerative medicine applications. In this study, MUSIi023-A iPSC line was generated from peripheral blood mononuclear cells of a Thai donor with 11 loci homozygous HLA. This cell line is free of viral integration, exhibited pluripotent stem cell characteristics and able to differentiate to all embryonic lineages.</div></div>","PeriodicalId":21843,"journal":{"name":"Stem cell research","volume":"88 ","pages":"Article 103840"},"PeriodicalIF":0.7,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145207691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Corrigendum to “Generation of the induced pluripotent stem cell line SJTUGHi004-A derived from a Best’s disease patient with c.763C > T mutation in BEST1 gene” [Stem Cell Res. 88 (2025) 103806] “从BEST1基因c.763C > T突变的Best病患者中提取的诱导多能干细胞系SJTUGHi004-A的产生”[干细胞研究,88(2025)103806]的勘误。
IF 0.7 4区 医学
Stem cell research Pub Date : 2025-09-30 DOI: 10.1016/j.scr.2025.103845
Yuxin Jiang , Hong Wang , Lihong Jiang , Jieqiong Chen , Tong Li , Guanran Zhang , Xueli Chen , Mei Jiang , Xiaodong Sun
{"title":"Corrigendum to “Generation of the induced pluripotent stem cell line SJTUGHi004-A derived from a Best’s disease patient with c.763C > T mutation in BEST1 gene” [Stem Cell Res. 88 (2025) 103806]","authors":"Yuxin Jiang ,&nbsp;Hong Wang ,&nbsp;Lihong Jiang ,&nbsp;Jieqiong Chen ,&nbsp;Tong Li ,&nbsp;Guanran Zhang ,&nbsp;Xueli Chen ,&nbsp;Mei Jiang ,&nbsp;Xiaodong Sun","doi":"10.1016/j.scr.2025.103845","DOIUrl":"10.1016/j.scr.2025.103845","url":null,"abstract":"","PeriodicalId":21843,"journal":{"name":"Stem cell research","volume":"89 ","pages":"Article 103845"},"PeriodicalIF":0.7,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145207663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Corrigendum to “Generation of a biallelic NRAP-knockout mutant from a human iPSC line”. [Stem. Cell Res. 88 (2025) 103829] “从人类iPSC系中产生双等位基因nrap敲除突变体”的勘误表。[茎。Cell Res. 88 (2025) 103829]
IF 0.7 4区 医学
Stem cell research Pub Date : 2025-09-30 DOI: 10.1016/j.scr.2025.103846
Janice Raabe , Vanessa Lewandowski , Sigrid Fuchs , Alexandra Hammerschmidt , Angelika Piasecki , Ellen Orthey , Elisabeth Krämer , Elisabeth Ehler , Friederike Cuello
{"title":"Corrigendum to “Generation of a biallelic NRAP-knockout mutant from a human iPSC line”. [Stem. Cell Res. 88 (2025) 103829]","authors":"Janice Raabe ,&nbsp;Vanessa Lewandowski ,&nbsp;Sigrid Fuchs ,&nbsp;Alexandra Hammerschmidt ,&nbsp;Angelika Piasecki ,&nbsp;Ellen Orthey ,&nbsp;Elisabeth Krämer ,&nbsp;Elisabeth Ehler ,&nbsp;Friederike Cuello","doi":"10.1016/j.scr.2025.103846","DOIUrl":"10.1016/j.scr.2025.103846","url":null,"abstract":"","PeriodicalId":21843,"journal":{"name":"Stem cell research","volume":"89 ","pages":"Article 103846"},"PeriodicalIF":0.7,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145183733","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Three modified human IPSC lines containing mutations in the distal DEHMBA associated locus of the SRCAP gene 包含SRCAP基因远端DEHMBA相关位点突变的三个修饰的人类IPSC系
IF 0.7 4区 医学
Stem cell research Pub Date : 2025-09-30 DOI: 10.1016/j.scr.2025.103847
Johannes Rhode , Lisa Hagenau , Stephanie Edwards , Falk F.R. Buettner , Ana Tzvetkova , Lars R. Jensen , Andreas W. Kuss
{"title":"Three modified human IPSC lines containing mutations in the distal DEHMBA associated locus of the SRCAP gene","authors":"Johannes Rhode ,&nbsp;Lisa Hagenau ,&nbsp;Stephanie Edwards ,&nbsp;Falk F.R. Buettner ,&nbsp;Ana Tzvetkova ,&nbsp;Lars R. Jensen ,&nbsp;Andreas W. Kuss","doi":"10.1016/j.scr.2025.103847","DOIUrl":"10.1016/j.scr.2025.103847","url":null,"abstract":"<div><div>We modified an existing human iPSC line (MHHi001-A), using CRISPR/Cas9, to introduce heterozygous frameshift mutations in a locus of the <em>SRCAP</em> gene that is associated with the DEHMBA disease (OMIM 619595). The modified iPSCs express several stem cell markers and are able to differentiate into cells originating from all three embryonic germ layers. No additional modifications or chromosomal defects were detected. The modified cells can serve as a model for the investigation of the involvement of SRCAP in DEHMBA (Developmental delay, Hypotonia, Musculoskeletal defects, and Behavioral Abnormalities) disease and/or its molecular functions in different cell types.</div></div>","PeriodicalId":21843,"journal":{"name":"Stem cell research","volume":"89 ","pages":"Article 103847"},"PeriodicalIF":0.7,"publicationDate":"2025-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145227821","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Generation and validation of a iPSC line from a healthy female donor using integration-free Sendai virus reprogramming 利用无整合仙台病毒重编程从健康女性供体中生成和验证iPSC系
IF 0.7 4区 医学
Stem cell research Pub Date : 2025-09-28 DOI: 10.1016/j.scr.2025.103844
Yuanyuan Chen, Xiaoqin Shi, Wei Long, Qing Shao, Haijiang Yu
{"title":"Generation and validation of a iPSC line from a healthy female donor using integration-free Sendai virus reprogramming","authors":"Yuanyuan Chen,&nbsp;Xiaoqin Shi,&nbsp;Wei Long,&nbsp;Qing Shao,&nbsp;Haijiang Yu","doi":"10.1016/j.scr.2025.103844","DOIUrl":"10.1016/j.scr.2025.103844","url":null,"abstract":"<div><div>We generated a characterized integration-free iPSC line derived via Sendai virus reprogramming from healthy female donor PBMCs. This method preserves genomic integrity and pluripotent potential. Validated markers include nuclear/membrane-bound OCT4, SOX2, NANOG/SSEA4, TRA-1–60 (qPCR/immunocytochemistry) and tri-lineage differentiation capacity confirmed via immunofluorescence (endodermal FOXA2/SOX17, mesodermal BRACHYURY/NKX2.5, ectodermal PAX6/NESTIN). Normal karyotype (46,XX) confirmed genetic stability. Sendai clearance by P10 and mycoplasma-negative status ensured biosafety. This non-integrative iPSC line provides a robust control resource for disease modeling and regenerative medicine.</div></div>","PeriodicalId":21843,"journal":{"name":"Stem cell research","volume":"89 ","pages":"Article 103844"},"PeriodicalIF":0.7,"publicationDate":"2025-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145227515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Generation of a transgenic pluripotent stem cell line expressing MMACHC protein harbouring the renal 2 thrombotic microangiopapathy-causing mutation (p.Q27R). 表达含有肾2血栓性微血管病变引起突变的MMACHC蛋白的转基因多能干细胞系的产生。
IF 0.7 4区 医学
Stem cell research Pub Date : 2025-09-22 DOI: 10.1016/j.scr.2025.103843
Zhiqi Zhou, Xiao'e Zhang, Cuilan Hou, Ying Wu
{"title":"Generation of a transgenic pluripotent stem cell line expressing MMACHC protein harbouring the renal 2 thrombotic microangiopapathy-causing mutation (p.Q27R).","authors":"Zhiqi Zhou, Xiao'e Zhang, Cuilan Hou, Ying Wu","doi":"10.1016/j.scr.2025.103843","DOIUrl":"https://doi.org/10.1016/j.scr.2025.103843","url":null,"abstract":"<p><p>Mutations in the MMACHC (metabolism of cobalamin associated C) gene are associated with cobalamin C disorder and have been reported in infants with renal thrombotic microangiopaopathy (TMA). The pathophysiology underlying these mutations and their role in causing endothelial damage remains elusive. We generated a transgenic pluripotent stem cell (iPSC) line expressing MMACHC protein harbouring the renal 2 thrombotic microangiopapathy-causing mutation (p.Q27R). The iPSC exhibits a typical stem cell morphology, expresses pluripotency markers, and displays a normal karyotype. The iPSC line provides a valuable platform for exploring the pathogenesis of endothelial injury induced by metabolism-mediated TMA and for screening drugs to reduce endothelial damage.</p>","PeriodicalId":21843,"journal":{"name":"Stem cell research","volume":"89 ","pages":"103843"},"PeriodicalIF":0.7,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145239624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Generation of a human iPSC line (NUMNi002-A) from a patient with nephrotic syndrome harboring an INF2 gene variant 从携带INF2基因变异的肾病综合征患者身上产生的人类iPSC细胞系(NUMNi002-A
IF 0.7 4区 医学
Stem cell research Pub Date : 2025-09-20 DOI: 10.1016/j.scr.2025.103842
Chikao Onogi , Akihito Tanaka , Kazuhiro Furuhashi , Asuka Horinouchi , Kumiko Fujieda , Jun Matsumoto , Keita Hattori , Akiko Owaki , Tomohiro Kawazoe , Akihisa Kato , Yu Watanabe , Eri Koshi-Ito , Kayaho Maeda , Hangsoo Kim , Noritoshi Kato , Itaru Kushima , Norio Ozaki , Shoichi Maruyama
{"title":"Generation of a human iPSC line (NUMNi002-A) from a patient with nephrotic syndrome harboring an INF2 gene variant","authors":"Chikao Onogi ,&nbsp;Akihito Tanaka ,&nbsp;Kazuhiro Furuhashi ,&nbsp;Asuka Horinouchi ,&nbsp;Kumiko Fujieda ,&nbsp;Jun Matsumoto ,&nbsp;Keita Hattori ,&nbsp;Akiko Owaki ,&nbsp;Tomohiro Kawazoe ,&nbsp;Akihisa Kato ,&nbsp;Yu Watanabe ,&nbsp;Eri Koshi-Ito ,&nbsp;Kayaho Maeda ,&nbsp;Hangsoo Kim ,&nbsp;Noritoshi Kato ,&nbsp;Itaru Kushima ,&nbsp;Norio Ozaki ,&nbsp;Shoichi Maruyama","doi":"10.1016/j.scr.2025.103842","DOIUrl":"10.1016/j.scr.2025.103842","url":null,"abstract":"<div><div>A variant of <em>INF2</em> has been identified as a risk factor for nephrotic syndrome (focal segmental glomerulosclerosis; FSGS). The mechanism by which this variant contributes to FSGS onset remains unclear. Furthermore, treatment for FSGS remains to be established.We generated induced pluripotent stem cells (iPSCs) from a patient with FSGS caused by the <em>INF2</em> variant. These iPSCs express stemness markers and can differentiate into the three germ layers <em>in vitro</em>. These iPSCs will be useful tools for understanding the pathophysiology of this type of FSGS and to screen the relevant treatment.</div></div>","PeriodicalId":21843,"journal":{"name":"Stem cell research","volume":"88 ","pages":"Article 103842"},"PeriodicalIF":0.7,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145118681","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Establishment of an induced pluripotent stem cell line (HMUCPi001-A) from a hypertrophic cardiomyopathy patient carrying MYBPC3 c.3072C > A mutation 从携带MYBPC3 c.3072C > a突变的肥厚性心肌病患者身上建立诱导多能干细胞系(HMUCPi001-A)
IF 0.7 4区 医学
Stem cell research Pub Date : 2025-09-20 DOI: 10.1016/j.scr.2025.103841
Dongping Liu , Mingyu Yang , Shasha Fan , Manyu Gong , Ying Zhang , Yanan Jiang , Siyu Wang , Hao Wang , Maomao Zhang , Ying Zhang
{"title":"Establishment of an induced pluripotent stem cell line (HMUCPi001-A) from a hypertrophic cardiomyopathy patient carrying MYBPC3 c.3072C > A mutation","authors":"Dongping Liu ,&nbsp;Mingyu Yang ,&nbsp;Shasha Fan ,&nbsp;Manyu Gong ,&nbsp;Ying Zhang ,&nbsp;Yanan Jiang ,&nbsp;Siyu Wang ,&nbsp;Hao Wang ,&nbsp;Maomao Zhang ,&nbsp;Ying Zhang","doi":"10.1016/j.scr.2025.103841","DOIUrl":"10.1016/j.scr.2025.103841","url":null,"abstract":"<div><div>Hypertrophic cardiomyopathy (HCM) is an inherited cardiovascular disorder characterized by left ventricular hypertrophy and an elevated risk of sudden cardiac death. Cardiac myosin binding protein C (<em>MYBPC3</em>) is the most frequently mutated gene leading to HCM. In this study, peripheral blood mononuclear cells isolated from an HCM patient harboring a heterozygous MYBPC3 missense mutation (c.3072C &gt; A; p.S1024R) were reprogrammed via Sendai virus vectors to generate a patient-specific induced pluripotent stem cell (iPSC) line. The iPSC line exhibits normal morphology and karyotype, alongside definitive hallmarks of pluripotency, including trilineage differentiation potential.</div></div>","PeriodicalId":21843,"journal":{"name":"Stem cell research","volume":"88 ","pages":"Article 103841"},"PeriodicalIF":0.7,"publicationDate":"2025-09-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145118679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Generation of a Flattop-T2A-H2B-Venus x C-peptide-mCherry double reporter human iPSC line to monitor WNT/Planar cell polarity pathway activity 构建flattp - t2a - h2b - venus x C-peptide-mCherry双报告细胞系,监测WNT/Planar细胞极性通路活性
IF 0.7 4区 医学
Stem cell research Pub Date : 2025-09-19 DOI: 10.1016/j.scr.2025.103838
Tobias Greisle , Ines Kunze , Xianming Wang , Andrzej R. Malinowski , Anika Böttcher , Heiko Lickert , Ingo Burtscher
{"title":"Generation of a Flattop-T2A-H2B-Venus x C-peptide-mCherry double reporter human iPSC line to monitor WNT/Planar cell polarity pathway activity","authors":"Tobias Greisle ,&nbsp;Ines Kunze ,&nbsp;Xianming Wang ,&nbsp;Andrzej R. Malinowski ,&nbsp;Anika Böttcher ,&nbsp;Heiko Lickert ,&nbsp;Ingo Burtscher","doi":"10.1016/j.scr.2025.103838","DOIUrl":"10.1016/j.scr.2025.103838","url":null,"abstract":"<div><div>Deriving functional β-cells from human induced pluripotent stem cells (hiPSCs) holds potential for cell replacement therapy, disease modeling, and drug testing in diabetes research. Wnt/Planar cell polarity (PCP) signaling is crucial for endocrine cell development and β-cell maturation in murine models and can be tracked by the expression of the tissue-specific effector gene <em>Flattop</em>. Here, we report the generation of a human fluorescent <em>FLTP</em>/<em>CFAP126</em> (Flattop-T2A-H2B-Venus) and FLTP-Insulin (Flattop-T2A-H2B-Venus x C-peptide-mCherry) double reporter by CRISPR/Cas9 gene editing. These hiPSC reporter lines allow monitoring of WNT/PCP signaling during endocrine cell formation and studying its role in β-cells in a human model system.</div></div>","PeriodicalId":21843,"journal":{"name":"Stem cell research","volume":"88 ","pages":"Article 103838"},"PeriodicalIF":0.7,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145118682","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Generation of a human induced pluripotent stem cell line (SDCHi005-A) from peripheral blood mononuclear cells of a patient with epilepsy 从癫痫患者外周血单个核细胞中生成人诱导多能干细胞系(SDCHi005-A)
IF 0.7 4区 医学
Stem cell research Pub Date : 2025-09-19 DOI: 10.1016/j.scr.2025.103839
Song Su , Ying Ren , Tong Zhang , Wandong Hu , Hongwei Zhang , Yong Liu , Chunhong Duan
{"title":"Generation of a human induced pluripotent stem cell line (SDCHi005-A) from peripheral blood mononuclear cells of a patient with epilepsy","authors":"Song Su ,&nbsp;Ying Ren ,&nbsp;Tong Zhang ,&nbsp;Wandong Hu ,&nbsp;Hongwei Zhang ,&nbsp;Yong Liu ,&nbsp;Chunhong Duan","doi":"10.1016/j.scr.2025.103839","DOIUrl":"10.1016/j.scr.2025.103839","url":null,"abstract":"<div><div>Epilepsy is a chronic cerebral disorder characterized by recurrent, episodic, and transient dysfunction of the central nervous system due to abnormal and excessive neuronal activity. In this study, peripheral blood mononuclear cells (PBMCs) were isolated from a young epilepsy patient carrying a DEP domain-containing 5 (DEPDC5) gene nonsense mutation, which was confirmed through clinical and genetic diagnosis. Induced pluripotent stem cells (iPSCs) were established using a non-integrating method carrying plasmids encoding OCT4, SOX2, KLF4, BCL-XL and C-MYC. The constructed iPSCs exhibited typical pluripotent cells morphology, normal karyotype, and demonstrated trilineage differentiation potential.</div></div>","PeriodicalId":21843,"journal":{"name":"Stem cell research","volume":"88 ","pages":"Article 103839"},"PeriodicalIF":0.7,"publicationDate":"2025-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145118678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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