Generation and validation of a iPSC line from a healthy female donor using integration-free Sendai virus reprogramming

IF 0.7 4区医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Stem cell research Pub Date : 2025-09-28 DOI:10.1016/j.scr.2025.103844

Yuanyuan Chen, Xiaoqin Shi, Wei Long, Qing Shao, Haijiang Yu

引用次数: 0

Abstract

We generated a characterized integration-free iPSC line derived via Sendai virus reprogramming from healthy female donor PBMCs. This method preserves genomic integrity and pluripotent potential. Validated markers include nuclear/membrane-bound OCT4, SOX2, NANOG/SSEA4, TRA-1–60 (qPCR/immunocytochemistry) and tri-lineage differentiation capacity confirmed via immunofluorescence (endodermal FOXA2/SOX17, mesodermal BRACHYURY/NKX2.5, ectodermal PAX6/NESTIN). Normal karyotype (46,XX) confirmed genetic stability. Sendai clearance by P10 and mycoplasma-negative status ensured biosafety. This non-integrative iPSC line provides a robust control resource for disease modeling and regenerative medicine.

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利用无整合仙台病毒重编程从健康女性供体中生成和验证iPSC系

我们通过仙台病毒重编程从健康女性供体pbmc中获得了一个特征的无整合iPSC系。这种方法保留了基因组的完整性和多能潜能。已验证的标记包括核/膜结合OCT4， SOX2, NANOG/SSEA4, TRA-1-60 （qPCR/免疫细胞化学）和通过免疫荧光证实的三系分化能力（内胚层FOXA2/SOX17，中胚层BRACHYURY/NKX2.5，外胚层PAX6/NESTIN）。正常核型（46，XX）证实遗传稳定性。仙台P10清除和支原体阴性状态确保了生物安全性。这种非整合性iPSC系为疾病建模和再生医学提供了强大的控制资源。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Stem cell research 生物-生物工程与应用微生物

CiteScore

2.20

自引率

8.30%

发文量

338

审稿时长

55 days

期刊介绍： Stem Cell Research is dedicated to publishing high-quality manuscripts focusing on the biology and applications of stem cell research. Submissions to Stem Cell Research, may cover all aspects of stem cells, including embryonic stem cells, tissue-specific stem cells, cancer stem cells, developmental studies, stem cell genomes, and translational research. Stem Cell Research publishes 6 issues a year.