Generation of a human induced pluripotent stem cell line (SDCHi005-A) from peripheral blood mononuclear cells of a patient with epilepsy

IF 0.7 4区医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Stem cell research Pub Date : 2025-09-19 DOI:10.1016/j.scr.2025.103839

Song Su , Ying Ren , Tong Zhang , Wandong Hu , Hongwei Zhang , Yong Liu , Chunhong Duan

引用次数: 0

Abstract

Epilepsy is a chronic cerebral disorder characterized by recurrent, episodic, and transient dysfunction of the central nervous system due to abnormal and excessive neuronal activity. In this study, peripheral blood mononuclear cells (PBMCs) were isolated from a young epilepsy patient carrying a DEP domain-containing 5 (DEPDC5) gene nonsense mutation, which was confirmed through clinical and genetic diagnosis. Induced pluripotent stem cells (iPSCs) were established using a non-integrating method carrying plasmids encoding OCT4, SOX2, KLF4, BCL-XL and C-MYC. The constructed iPSCs exhibited typical pluripotent cells morphology, normal karyotype, and demonstrated trilineage differentiation potential.

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从癫痫患者外周血单个核细胞中生成人诱导多能干细胞系（SDCHi005-A）

癫痫是一种慢性大脑疾病，其特征是由于异常和过度的神经元活动引起中枢神经系统的复发性、发作性和短暂性功能障碍。本研究从一例年轻癫痫患者外周血单个核细胞（PBMCs）中分离出携带DEP结构域5 （DEPDC5）基因无义突变，并通过临床和遗传学诊断予以证实。采用非整合方法建立了诱导多能干细胞（iPSCs），其质粒编码OCT4、SOX2、KLF4、BCL-XL和C-MYC。构建的iPSCs具有典型的多能细胞形态、正常的核型和三期分化潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Stem cell research 生物-生物工程与应用微生物

CiteScore

2.20

自引率

8.30%

发文量

338

审稿时长

55 days

期刊介绍： Stem Cell Research is dedicated to publishing high-quality manuscripts focusing on the biology and applications of stem cell research. Submissions to Stem Cell Research, may cover all aspects of stem cells, including embryonic stem cells, tissue-specific stem cells, cancer stem cells, developmental studies, stem cell genomes, and translational research. Stem Cell Research publishes 6 issues a year.