Establishment of the integration-free Class I and Class II 11 loci homozygous HLA iPSC line (MUSIi023-A) from peripheral blood mononuclear cells of a Thai donor

IF 0.7 4区医学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Stem cell research Pub Date : 2025-10-01 DOI:10.1016/j.scr.2025.103840

Nittaya Jiamvoraphong , Pattaraporn Nimsamer , Thidathip Wongsurawat , Chanchao Lorthongpanich , Chuti Laowtammathron , Surapol Issaragrisil

引用次数: 0

Abstract

Pluripotent stem cell (PSC)-based technologies offer a promising new approach to generating functional cells or grafts for transplantation. To minimize the risk of immune rejection, induced pluripotent stem cells (iPSCs) that are homozygous for HLA Class I and Class II genes are a potential solution to reduce the risk of immune rejection in regenerative medicine applications. In this study, MUSIi023-A iPSC line was generated from peripheral blood mononuclear cells of a Thai donor with 11 loci homozygous HLA. This cell line is free of viral integration, exhibited pluripotent stem cell characteristics and able to differentiate to all embryonic lineages.

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从泰国供者外周血单个核细胞中建立无整合ⅰ类和ⅱ类11位点纯合HLA iPSC系（MUSIi023-A）。

基于多能干细胞（PSC）的技术提供了一种有前途的新方法来产生用于移植的功能细胞或移植物。为了最大限度地降低免疫排斥的风险，在再生医学应用中，具有HLA I类和HLA II类基因纯合的诱导多能干细胞（iPSCs）是降低免疫排斥风险的潜在解决方案。在这项研究中，MUSIi023-A iPSC系是由具有11个位点纯合HLA的泰国供者的外周血单个核细胞产生的。该细胞系没有病毒整合，表现出多能干细胞特征，能够分化为所有胚胎谱系。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Stem cell research 生物-生物工程与应用微生物

CiteScore

2.20

自引率

8.30%

发文量

338

审稿时长

55 days

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