Sheng wu gong cheng xue bao = Chinese journal of biotechnology最新文献

{"title":"[Establishment and application of chemically inducible chromosomal evolution in <i>Escherichia coli</i> Nissle 1917].","authors":"Yumei Liu, Qingli Zhang, Lijun Shao, Xiaojing Liu, Xiaoli Yu","doi":"10.13345/j.cjb.240252","DOIUrl":"https://doi.org/10.13345/j.cjb.240252","url":null,"abstract":"<p><p>The probiotic strain <i>Escherichia coli</i> Nissle 1917 (EcN) with high biocompatibility and susceptibility to genetic modification is often applied in bacterial therapies for cancer. However, most studies have used plasmids as vectors to construct engineering strains from EcN. Plasmid-based expression systems suffer from genetic instability, and they need antibiotic selective pressure to maintain high copy number. This study aimed to employ EcN for synthesizing the photosensitizer 5-aminolevulinic acid (5-ALA). Firstly, the key genes of 5-ALA synthesis, <i>hemA</i>^M and <i>hemL</i>, were integrated into the EcN genome by the phage integration technique. Then, chemically inducible chromosomal evolution (CIChE) was adopted to increase the copy number of <i>hemA</i>^M and <i>hemL</i> and thus improved the stable synthesis of 5-ALA. The <i>in vitro</i> cell experiments verified that the constructed engineering strain can deliver stably synthesized 5-ALA to tumor cells and inhibit their growth. This study provided a basis for applying the engineering strains of EcN in the photodynamic therapy for tumors.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"40 12","pages":"4594-4604"},"PeriodicalIF":0.0,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142897171","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Microorganism-mediated arsenic reduction and its environmental effects].","authors":"Teng Mao, Guoliang Chen, Zhihui Qu","doi":"10.13345/j.cjb.240529","DOIUrl":"https://doi.org/10.13345/j.cjb.240529","url":null,"abstract":"<p><p>Arsenic (As) is a common toxic pollution element. The microorganism-mediated transformation of arsenic forms is an important part in the biogeochemical cycle of As. In the various microbial metabolic processes involving As, the coupling reduction of As has a great impact on the environment and is a process that is easily overlooked. From the biogeochemical cycle of As, this review introduces the microorganism-mediated methane oxidation, anaerobic ammonium oxidation, and iron (Fe)-sulfur (S) oxidation coupled with As reduction. Organic matter, pH, and redox potential are the main factors affecting the coupling reduction. After the coupling reduction, the toxicity and migration of As are greatly enhanced, which may increase the risk of As pollution. Therefore, it is of great significance to clarify the influences of carbon, nitrogen, Fe, S and other elements on the coupling process and explore more microbial processes coupled with As reduction for the prevention and control of As pollution.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"40 12","pages":"4480-4492"},"PeriodicalIF":0.0,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142897187","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Research progress in animal embryo implantation and endometrial organoids].","authors":"Jingyi Tu, Changqing Shen, Ruiling Lei, Jie Yang, Shicheng Wang, Siqi Peng, Lang Li, Xiaoyan Qiu","doi":"10.13345/j.cjb.240128","DOIUrl":"https://doi.org/10.13345/j.cjb.240128","url":null,"abstract":"<p><p>Embryo implantation involves a complex interaction between the embryo and the endometrium of the mother, the study of which faces a variety of problems. The modeling of endometrial epithelial organoids and endometrial assembloids provides a new way to study the process of embryo implantation <i>in vitro</i>. This paper summarized the latest research progress in embryo implantation, the regulation mechanism of endometrial receptivity by estrogen- progesterone coordination and embryo-derived signals, the establishment of endometrial organoids, and the development and application of endometrial assembloids in the research on mother-embryo interaction, providing new strategies for studying the communication between embryo and maternal uterus during implantation.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"40 12","pages":"4452-4466"},"PeriodicalIF":0.0,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142897276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Research progress in Argonaute proteins of vertebrates].","authors":"Xianhua Xie, Meng Ru, Jianling Peng, Linjian Weng, Jianzhen Huang","doi":"10.13345/j.cjb.240026","DOIUrl":"https://doi.org/10.13345/j.cjb.240026","url":null,"abstract":"<p><p>Argonaute proteins are active throughout the lifetime in a variety of organisms and they bind to small RNAs (sRNAs) to regulate gene expression. The Argonaute proteins of vertebrates can be classified into two clades: the Ago clade and the Piwi clade. Both clades have N, L1, L2, PAZ, MID and PIWI domains. The N domain is involved in the loading of sRNAs. L1 and L2 domains facilitate the linking between domains. The PAZ and MID domains exert functions by anchoring sRNAs. The PIWI domain of some Argonaute proteins has RNase H-like structure and exerts the endonuclease function. Ago proteins regulate gene expression at transcriptional and post-transcriptional levels. Piwi proteins mainly exist in the germ cells, silencing transposons in different ways to keep genome integrality and regulating gene expression. In recent years, great progress has been made in Argonaute proteins in terms of the crystal structures, functions, and expression patterns. By reviewing the relevant studies, we elaborate on the structures, sRNA dependence, gene expression regulation, and biological roles of the Ago and Piwi proteins in vertebrates, aiming to clarify the roles of Argonaute proteins in epigenetic regulation and provide a reference for further research and application of these proteins.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"40 12","pages":"4418-4438"},"PeriodicalIF":0.0,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142897284","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Research progress in biological activities and oocyte aging-regulating effect of EGCG].","authors":"Weiying Zhang, Huizhu Zhang, Yujun Li, Daoliang Lan, Xianrong Xiong, Yaying Wang, Jian Li, Honghong He","doi":"10.13345/j.cjb.240192","DOIUrl":"https://doi.org/10.13345/j.cjb.240192","url":null,"abstract":"<p><p>Epigallocatechin gallate (EGCG), the predominant polyphenol in green tea, exerts a spectrum of physiological activities, including antioxidant, anticancer, and anti-inflammatory effects. Emerging research underscores the significance of EGCG in modulating oocyte aging. EGCG can enhance antioxidant defenses, improve mitochondrial functions, and inhibit apoptotic pathways, thereby retarding the aging of oocytes. This review delineates the main molecular features of EGCG and expounds its regulatory mechanisms concerning oocyte aging, enriching the knowledge on the role of EGCG in the amelioration of oocyte aging.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"40 12","pages":"4382-4395"},"PeriodicalIF":0.0,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142897287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Research progress in the adsorption of heavy metal ions from wastewater by modified biochar].","authors":"Jing Hong, Yongyong Dai, Qijun Nie, Zhiqiang Liao, Liangcai Peng, Dan Sun","doi":"10.13345/j.cjb.240263","DOIUrl":"https://doi.org/10.13345/j.cjb.240263","url":null,"abstract":"<p><p>The rapid development of modern industries is accompanied with the aggravating water heavy metal pollution, which poses a potential threat to the aquatic environment and the health of local populations. As an efficient and economical adsorbent, biochar demonstrates the adsorption capacity for heavy metal ions and its adsorption capacity is significantly enhanced after modification. Therefore, biochar can effectively mitigate environmental pollution. By reviewing the existing studies, we summarize the modification methods of biochar, compare the advantages and disadvantages of physical, biological, and chemical modification methods, analyze the effects of modification on the adsorption capacity of biochar for heavy metal ions, and expound the modification mechanism of biochar. On this basis, this article puts forward the future research directions of the application of biochar in treating coexisting pollutants, aiming to provide a reference for the application of biochar in the purification of heavy metal-containing wastewater.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"40 12","pages":"4467-4479"},"PeriodicalIF":0.0,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142897328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Preparation and methodological analysis of chitosan-based ultrasound-coupled hydrogel pads].","authors":"Dan Chen, Jiani Yuan, Xiaojun Deng, Lei Ding, Zhongwei An, Wen Luo","doi":"10.13345/j.cjb.240586","DOIUrl":"https://doi.org/10.13345/j.cjb.240586","url":null,"abstract":"<p><p>This study aims to optimize the process for preparing chitosan-based ultrasound-coupled hydrogel pads and investigate their application potential in ultrasonography. Chitosan, 2-acrylamido-2-methylpropanesulfonic acid, and N-isopropylacrylamide were used as the main materials to prepare chitosan-based ultrasound-coupled hydrogel pads. The free-radical polymerization conditions were optimized by a three-factor, three-level orthogonal test with the tensile strength and ultrasound image quality of the hydrogel pads as evaluation indicators. The optimal prescription was selected by optimizing three factors of raw material ratio, polymerization temperature, and freeze-drying time. The structure and performance of the hydrogel pads were characterized by a scanning electron microscope, a universal testing machine, and an ultrasonic diagnostic instrument. The results showed that the optimal prescription was as follows: the chitosan: 2-acrylamide-2-methylpropanesulfonic acid: N-isopropylacrylamide ratio of 2:0.55:17.27, the polymerization temperature of 25 ℃, and the freeze-drying time of 48 h. The ultrasonically-coupled hydrogel pads prepared under these conditions were transparent, with a porous structure, good adhesion, and high tensile strength. The hydrogel pads had good swelling properties and the swelling degree decreased slowly on day 10. The quality of the ultrasound images obtained <i>via</i> chitosan-based hydrogel pads was not significantly different from that obtained <i>via</i> medical ultrasound coupling agent. In this study, we analyzed the effects of different preparation processes on the gel formation of chitosan-based ultrasound-coupled hydrogel pads. The hydrogel pads were transparent and mild and non-irritating to the human body, serving as an ultrasound transmission material for ultrasonography.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"40 12","pages":"4586-4593"},"PeriodicalIF":0.0,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142897263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Progress and prospects of the effects and mechanisms of myokines in regulating fiber type transition of skeletal muscle].","authors":"Boyu Huang, Ziyi Zhang, Weijun Pang","doi":"10.13345/j.cjb.240729","DOIUrl":"https://doi.org/10.13345/j.cjb.240729","url":null,"abstract":"<p><p>The fiber type transition of skeletal muscle is an intricate and essential physiological process in the body, significantly influencing both the function and metabolism of skeletal muscle. This phenomenon is not only affected by external environmental changes but also intricately regulated by internal physiological mechanisms. Therefore, exploring the physiological process of muscle fiber type transition holds considerable significance for the treatment of human neuromuscular disorders and the improvement of meat quality in livestock and poultry. It has been discovered that the cytokines secreted by skeletal muscle, <i>i</i>.<i>e</i>., myokines, play a role in the fiber type transition of skeletal muscle. Myokines mainly act on skeletal muscle in autocrine and paracrine forms to participate in signal transduction and regulate the fiber type transition of skeletal muscle. This paper reviews the functional differences among various muscle fiber types, expounds the effects and mechanisms of myokines in regulating the transition processes of these fiber types, and prospects the future research directions in this field. This review is expected to provide theoretical support for enhancing the meat quality of livestock and poultry and treating skeletal muscle-related diseases.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"40 12","pages":"4365-4381"},"PeriodicalIF":0.0,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142897267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Research progress in polysaccharide-modified lipid nanoparticles for drug delivery].","authors":"Yuqing Ma, Haiyun Liu, Xiaoqiang Wang, Shiqi Sun, Huichen Guo","doi":"10.13345/j.cjb.240188","DOIUrl":"https://doi.org/10.13345/j.cjb.240188","url":null,"abstract":"<p><p>Lipid nanoparticles serve as a promising drug delivery system due to the good biocompatibility, non-immunogenicity, and high drug loading efficiency. However, unmodified lipid nanoparticles have limitations such as poor stability, easy hydrolysis, and rapid removal. To overcome these shortcomings, researchers have developed peptide modification, antibody modification, ligand modification, nucleic acid aptamer modification, and polysaccharide modification for lipid nanoparticles. Polysaccharides are a class of natural polymers, and the polysaccharide-modified lipid nanoparticles exhibit good biocompatibility, precise targeting, and low toxicity. Therefore, polysaccharide-modified lipid nanoparticles demonstrate great potential in clinical treatment. This review summarizes the preparation and application of polysaccharide-modified lipid nanoparticles, aiming to provide a reference for further research and development of new lipid nanoparticles.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"40 12","pages":"4339-4350"},"PeriodicalIF":0.0,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142897306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Characterization of host factors <i>ARF4</i> and <i>ARF5</i> upon Zika virus infection <i>in vivo</i> by construction of gene knockout mice].","authors":"Kao Deng, Mingyuan Li, Huiying Zhang, Yongqiang Deng, Yuan Qin, Chengfeng Qin","doi":"10.13345/j.cjb.240307","DOIUrl":"https://doi.org/10.13345/j.cjb.240307","url":null,"abstract":"<p><p>The effects of host factors ADP-ribosylation factor 4 (ARF4) and ADP-ribosylation factor 5 (ARF5) upon Zika virus (ZIKV) infection <i>in vivo</i> were characterized by construction of gene knockout mice via CRISPR-Cas9. Firstly, <i>ARF5</i> and <i>ARF4</i> genes were modified by the CRISPR-Cas9 system and then microinjected into the fertilized eggs of C57BL/6JGpt mice. Fertilized eggs were transplanted to obtain <i>ARF4</i> or <i>ARF5</i> knockout (ARF4KO or ARF5KO) mice, and <i>ARF4</i>/<i>5</i> double knockout mice were achieved by the mating between ARF4KO and ARF5KO mice (ARF4KO/ARF5KO). Then, the mouse genotypes were identified by PCR to identify the positive knockout mice, and RT-qPCR was employed to examine the knockout efficiency. The mice were then infected with ZIKV and the blood and tissue samples were collected after 2, 4, and 6 days. RT-qPCR was then employed to determine the virus load, and hematoxylin-eosin staining was employed to observe the pathological changes in the tissue. The results showed that expected PCR bands were detected from ARF4KO^-/+, ARF5KO^-/-, and ARF4KO^-/+/ARF5KO^-/- mice, respectively. The results of mRNA transcription measurement indicated the significant knockdown of <i>ARF4</i> by 37.8%-50.0% but not <i>ARF5</i> in ARF4KO^-/+ compared with the wild-type mice. Meanwhile, complete knockout of <i>ARF5</i> and no changes in <i>ARF4</i> were observed in ARF5KO^-/- mice. Additionally, completed knockout of <i>ARF5</i> and down-regulated mRNA level of <i>ARF4</i> in the lung, kidney, and testis were detected in ARF4KO^-/+/ARF5KO^-/-mice in comparison with the wild-type mice. The virus load in the serum decreased in ARF4KO^-/+ mice, while it showed no significant change in ARF5KO^-/- or ARF4KO^-/+/ARF5KO^-/- mice compared with that in the wild type. Meanwhile, ARF4KO^-/+ mice showcased no significant difference in virus load in various tissues but attenuated pathological changes in the brain and testis compared with the wild-type mice. We successfully constructed ARF4KO and ARF5KO mice by CRISPR-Cas9 in this study. ARF4 rather than ARF5 is essential for ZIKV infection <i>in vivo</i>. This study provided animal models for studying the roles of ARF4 and ARF5 in ZIKV infection and developing antivirals.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"40 12","pages":"4605-4615"},"PeriodicalIF":0.0,"publicationDate":"2024-12-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142897065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}