Sheng wu gong cheng xue bao = Chinese journal of biotechnology最新文献

{"title":"[Mild solubilization, purification, and immunoreactivity characterization of VP1 inclusion body protein of serotype O foot-and-mouth disease virus based on sodium lauroyl sarcosinate].","authors":"Lei Fan, Jianpeng Liang, Jieshan Lin, Linlin Cai, Tao Ren","doi":"10.13345/j.cjb.260409","DOIUrl":"https://doi.org/10.13345/j.cjb.260409","url":null,"abstract":"<p><p>To efficiently prepare the capsid protein VP1 of serotype O foot-and-mouth disease virus (FMDV) with favorable immunoreactivity and to address the challenges that VP1 is prone to form inclusion bodies in the <i>Escherichia coli</i> expression system and is difficult to refold, this study developed a novel process based on sodium lauroyl sarcosinate (LSN) for the mild solubilization and chromatographic purification of FMDV VP1 inclusion body protein. First, the recombinant SUMO-VP1 protein was expressed in <i>E</i>. <i>coli</i> by induction, and Western blotting results revealed that the recombinant SUMO-VP1 protein was expressed at a high level; however, even in the presence of the SUMO solubility-enhancing tag, a large proportion of the protein still existed in the form of insoluble inclusion bodies, demonstrating the extremely hydrophobic folding propensity of the VP1 protein. Second, different concentrations of LSN were used to screen for mild solubilization conditions for the inclusion bodies. SDS-PAGE and grayscale analysis indicated that the solubilization rate exhibited a significant dose-dependent increase with rising LSN concentration, and 1.0% LSN was identified as the optimal working concentration for mild solubilization, at which the relative solubilization rate of the target protein reached as high as 92%. Subsequently, to avoid steric hindrance interference from the macromolecular tag, this 1.0% LSN solubilization process was applied to the extraction of SUMO-tag-free recombinant His-VP1 inclusion bodies, and the solubilized recombinant His-VP1 protein was purified using Ni²⁺-TED affinity chromatography, which successfully removed background contaminating proteins and yielded recombinant His-VP1 protein with a purity over 90%. Finally, the purified and desalted recombinant His-VP1 protein was used as the coating antigen in an indirect ELISA. The results demonstrated that the recombinant protein could be recognized by specific antibodies against VP1 of serotype O FMDV, and the binding curve exhibited a typical smooth sigmoidal shape and a significant dose-dependent effect, confirming that this process did not compromise the protein structure and fully preserved its native immunoreactivity. In conclusion, the LSN-based mild solubilization and chromatographic purification process developed in this study successfully circumvents the destructive effects and complex refolding steps associated with conventional strong denaturants, and the prepared recombinant His-VP1 protein possesses the potential to serve as a high-quality serological diagnostic antigen. This mild solubilization process may also provide a technical reference for the preparation of other highly hydrophobic and poorly soluble recombinant antigens.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"42 8","pages":"3541-3549"},"PeriodicalIF":0.0,"publicationDate":"2026-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148813906","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Regulatory role of miR-205-5p in the proliferation, differentiation and muscle atrophy of C2C12 myoblasts].","authors":"Yulin He, Wangxi Chai, Jiaqi Yue, Wenqing Wang, Taiyong Yu","doi":"10.13345/j.cjb.260085","DOIUrl":"https://doi.org/10.13345/j.cjb.260085","url":null,"abstract":"<p><p>The growth and development of skeletal muscle are closely associated with the proliferation, differentiation, and fusion of myoblasts. MicroRNAs (miRNAs) are a class of non-coding RNAs approximately 22 nucleotides in length that regulate skeletal muscle formation. miR-205-5p has been implicated in various physiological processes and diseases; however, its role in muscle development remains unclear. Therefore, this study used C2C12 myoblasts as a model to investigate the regulatory effects of miR-205-5p on myoblast proliferation, differentiation, and muscle atrophy, and to preliminarily elucidate its role in maintaining skeletal muscle homeostasis. The results showed that overexpression of miR-205-5p significantly inhibited the proliferation of C2C12 myoblasts while promoting their differentiation (<i>P</i>0.05), whereas interference with miR-205-5p yielded opposite results. In both the muscle atrophy model established after 8 days of induced differentiation and the dexamethasone-induced muscle atrophy model, overexpression of miR-205-5p alleviated the occurrence of muscle atrophy, whereas suppression of miR-205-5p expression exacerbated muscle atrophy (<i>P</i>0.05). In conclusion, miR-205-5p contributes to the maintenance of skeletal muscle homeostasis during muscle development by inhibiting proliferation, promoting differentiation, and attenuating muscle atrophy. This study provides a new molecular basis for elucidating the regulatory mechanisms of microRNAs in muscle development and for muscle atrophy intervention research.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"42 8","pages":"3580-3592"},"PeriodicalIF":0.0,"publicationDate":"2026-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148813970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Development and application of a triplex basic- recombinase polymerase amplification method to detect <i>Brucella</i>, <i>Babesia</i>, and <i>Pasteurella multocida</i> in cattle].","authors":"Zhuoqun Xu, Yanru Huang, Xu Xu, Shanshan Chu, Yan Chen, Jie Zhou, Keyu Li, Jianzhong Han, Daofeng Qu","doi":"10.13345/j.cjb.250754","DOIUrl":"https://doi.org/10.13345/j.cjb.250754","url":null,"abstract":"<p><p>To combat three major pathogens-<i>Brucella</i> spp.<i>,</i><i>Babesia bovis</i>, and <i>Pasteurella multocida</i>-threatening cattle health, we developed a rapid and accurate triplex basic-recombinase polymerase amplification (Basic-RPA) detection system. Targeting <i>bcsp31</i>, <i>rap-1</i>, and <i>kmt1</i>, we designed and screened specific RPA primers and optimized the reaction temperature, time, and primer concentration. The best reaction conditions were 38 ℃ for 25 min, with primers at 10 μmol/L in a 1:1:1 ratio. We then used reference and non-target strains to evaluate the specificity of the established method and serial DNA dilutions to evaluate the method sensitivity and repeatability. Subsequently, we applied the method to detection of 640 samples collected from cattle farms, slaughterhouses, supermarkets, and wet markets. The limit of detection of this method for all the three pathogens reached 10³ CFU/mL (copies/mL). The method showed high specificity, excellent repeatability, and 100% consistency with PCR results. Among the samples, <i>Brucella</i> spp., <i>B</i>. <i>bovis</i>, and <i>P</i>. <i>multocida</i> were detected at rates of 0.31%, 2.34%, and 1.25%, respectively, indicating different patterns of pathogen distribution across production stages. In conclusion, our triplex Basic-RPA method is simple, sensitive, and highly specific. It provides an efficient molecular tool for rapid detection and surveillance of bovine pathogens along the farm-to-market chain and lays the groundwork for developing advanced RPA-based technologies such as recombinase polymerase amplification-lateral flow dipstick (RPA-LFD) and recombinase polymerase amplification-clustered regularly interspaced short palindromic repeats, (RPA-CRISPR).</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"42 8","pages":"3723-3735"},"PeriodicalIF":0.0,"publicationDate":"2026-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148814103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Research progress in phage therapy against enterotoxigenic <i>Escherichia coli</i> infection in animals].","authors":"Wenzhuo Jia, Shujiao Zhu, Fenghua Dong, Hui Zhang, Hongduo Bao, Yanhua Li, Yan Zhou","doi":"10.13345/j.cjb.260230","DOIUrl":"https://doi.org/10.13345/j.cjb.260230","url":null,"abstract":"<p><p>Enterotoxigenic <i>Escherichia coli</i> (ETEC) is a major causative agent of animal diarrhea, severely constraining the sustainable development of animal husbandry and endangering food safety and human health. Driven by the worsening antibiotic resistance crisis and the policy implementation of antibiotic reduction and replacement, phages have attracted extensive attention as novel antibacterial candidates. With prominent strengths including rapid bactericidal action, strict host specificity, no disturbance to intestinal microflora, and no drug residues, phages exhibit promising prospects for antibacterial development. This review summarizes the pathogenic mechanisms of ETEC and research progress in the antibacterial applications of ETEC phages, with a focus on the clinical effects of phage intervention against diarrhea in piglets, calves, and lambs. Furthermore, it outlines the research progress in the application of phages in animal-derived food preservation and bacterial biofilm elimination. Additionally, this paper delves into the co-evolutionary infection-defense mechanism between phages and bacteria, aiming to offer a reference for the clinical translation and application of ETEC phage therapy.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"42 8","pages":"3419-3436"},"PeriodicalIF":0.0,"publicationDate":"2026-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148814042","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Dual-axis evolution model of physical intervention-bioprinting depth for <i>in situ</i> 3D bioprinting <i>in vivo</i> and research perspectives].","authors":"Hengrui Zhang, Zhaoliang Jiang, Weifeng Zhang, Lichao Gong, Siliang Wang, Jie Chen","doi":"10.13345/j.cjb.260339","DOIUrl":"https://doi.org/10.13345/j.cjb.260339","url":null,"abstract":"<p><p><i>In situ</i> 3D bioprinting <i>in vivo</i> is leading a profound paradigm shift of manufacturing in regenerative medicine. However, to achieve the leap from mere structural replication to complex functional reconstruction, current technologies urgently need to overcome the intrinsic engineering barrier of deep adaptation to the dynamic <i>in vivo</i> microenvironment. To address this challenge, this review proposes for the first time a dual-axis evolutionary theoretical model of degree of physical intervention-bioprinting depth. This model systematically categorizes the technological trajectory into three stages: macroscopic morphological remodeling in open environments (stage 1), flexible interventional shaping within restricted cavities (stage 2), and non-contact energy field-controlled assembly (stage 3). Drawing upon deep practices in interdisciplinary fields such as dynamic mixing control of multiphase fluids, machine learning-driven deformation compensation, and biomimetic porous gradient structure design, this review highlights the core supporting roles of multimodal perception, physiological motion compensation, and artificial intelligence closed-loop control in enhancing <i>in vivo</i> manufacturing precision. Furthermore, it systematically summarizes the preclinical validation outcomes of each evolutionary stage in the repair of typical tissues, including bone, cartilage, skin, and internal organs. This dual-axis model not only establishes systematic theoretical coordinates to resolve the fragmentation of current technological routes, but also delineates a comprehensive roadmap for interdisciplinary researchers to overcome the engineering bottlenecks in translating laboratory proof-of-concept into intelligent clinical devices. In view of the translational barriers such as deep-tissue safety evaluation and technological standardization, this review prospectively points out that future endeavors should rely on the integration of multimodal physical fields and digital twins to break the limitations of single materials. By focusing on the <i>in situ</i> precise construction of complex heterogeneous tissues, the manufacturing paradigm will comprehensively evolve towards cell-free <i>in situ</i> induction, thereby providing an ultimate medical solution for end-stage tissue defects based on an <i>in vivo</i> miniature autonomous repair factory.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"42 8","pages":"3463-3477"},"PeriodicalIF":0.0,"publicationDate":"2026-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148814089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Multidimensional characterization of biofilm formation promoted by <i>Mycobacterium tuberculosis</i> Rv1904].","authors":"Zhongting Ji, Lihan Yin, Yuqing Xie, Zhengzhong Xu, Chengkun Zheng, Xin'an Jiao, Xiang Chen","doi":"10.13345/j.cjb.260025","DOIUrl":"10.13345/j.cjb.260025","url":null,"abstract":"<p><p><i>Mycobacterium tuberculosis</i> (<i>M</i>. <i>tb</i>), the causative agent of tuberculosis, forms biofilms that contribute significantly to enhanced drug resistance. This study aimed to investigate the role of Rv1904 in the biofilm formation of <i>M</i>. <i>tb</i>. Recombinant strains were constructed for both <i>Mycobacterium smegmatis</i> and <i>M</i>. <i>tb</i>, followed by systematic biological characterization in terms of colony morphology, biofilm-forming ability, sliding motility and aggregation phenotypes, transcription levels of specific genes, tolerance to environmental stresses, intracellular survival capacity, and susceptibility to multiple compounds. The results demonstrated that Rv1904 markedly promoted biofilm formation, upregulated the transcription of the fatty acid synthesis gene <i>fabg4</i>, and enhanced bacterial tolerance to abiotic stresses such as temperature fluctuations, oxidative damage, and nutrient deprivation. These effects collectively improved intracellular survival within host cells. Among the seven tested compounds, thioacetazone (TB1) exhibited the strongest inhibitory effect on biofilm formation. In conclusion, Rv1904 enhances the environmental adaptation and survival advantage of <i>M</i>. <i>tb</i> by promoting biofilm formation, whereas TB1 effectively suppresses Rv1904-mediated biofilm formation. This study reveals the critical role of Rv1904 in biofilm regulation and provides a potential new target for the development of anti-tuberculosis drugs targeting biofilms.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"42 7","pages":"3217-3228"},"PeriodicalIF":0.0,"publicationDate":"2026-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148607480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Optimization of the enzymatic hydrolysis process of β-agarase Y3R1 from <i>Catenovulum agarivorans</i> and effects of the products on intestinal microbiota during <i>in vitro</i> fermentation].","authors":"Hebin Li, Linglong Huang, Jianbo Li, Mengmeng Lan, Wenkai Cai, Yanbing Zhu","doi":"10.13345/j.cjb.260084","DOIUrl":"10.13345/j.cjb.260084","url":null,"abstract":"<p><p>This study aimed to optimize the hydrolysis conditions of agarose by β-agarase Y3R1 and to investigate the prebiotic effects of its product, neoagarooligosaccharides, on gut microbiota. Using reducing sugar yield as the evaluation index, the enzymatic hydrolysis process of β-agarase Y3R1 from <i>Catenovellum agarivorans</i> was optimized. The content of reducing sugar and total sugar generated during the reaction were determined <i>via</i> the 3,5-dinitrosalicylic acid (DNS) method and the phenol-sulfuric acid method, respectively, and the average degree of polymerization (DP) of the enzymatic hydrolysis products was calculated. Through response surface methodology, the conditions for β<i>-</i>agarase hydrolysis of agarose were optimized as follows: a 50 mL reaction system, an agarose (substrate) concentration of 41.9 mg/mL, an enzyme dosage of 7.5 U, and reaction conducted at 65 ℃ and pH 6.5 for 120 min. The products of agarose hydrolysis by β<i>-</i>agarase Y3R1 were identified by MS and HPLC as neoagarobiose, neoagarotetraose, and neoagarohexaose, with the peak area ratio of 4%:77%:17%. Furthermore, an <i>in vitro</i> fermentation model was employed to investigate the regulatory effects of neoagaro-oligosaccharides (NAOS) on intestinal microbiota through alpha and beta diversity analyses, comparison of intestinal microbiota structure at the phylum and genus levels, LEfSe, and short-chain fatty acid (SCFA) content determination. The results indicated that NAOS primarily modulated the intestinal microbiota structure by promoting the growth of beneficial bacteria such as <i>Bacteroides</i> and <i>Limosilactobacillus</i>, while also exerting prebiotic effects through changes in levels of SCFAs, especially acetic acid, propionic acid, and butyric acid. The above findings confirm the promising prebiotic potential of NAOS and provide a theoretical basis for its application in functional foods.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"42 7","pages":"3038-3058"},"PeriodicalIF":0.0,"publicationDate":"2026-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148607492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Reform and practice of innovative bioengineering undergraduate talent cultivation model driven by demand and multiple dimensions].","authors":"Dan Zeng, Junfeng Hui, Shihong Shen, Huan Lei, Sen Zhang, Daidi Fan","doi":"10.13345/j.cjb.250619","DOIUrl":"10.13345/j.cjb.250619","url":null,"abstract":"<p><p>Against the backdrop of emerging engineering education and in response to the discrepancy between rapid industrial growth and the shortage of innovative talent supply, the bioengineering program at Northwest University has systematically restructured its innovative talent cultivation model. Guided by industry development trends and regional industrial upgrading, the program integrates science with engineering through the outcome-based education (OBE) philosophy of student-centeredness, output-orientation, and continuous improvement. We have established a comprehensive training model oriented toward the biomanufacturing industrial chain and characterized by a three-level, four-module, and five-dimensional guidance framework. This model integrates hierarchical training (basic, comprehensive, and innovative), practical modules (basic skills, professional skills, innovative experiments, and social practice), and a five-dimensional mentorship system comprising counselors, class sponsors, academic tutors, entrepreneurship mentors, and enterprise mentors. Supported by a robust teaching quality assurance system, this initiative has fostered a new educational ecosystem that deeply incorporates research with teaching and integrates production, education, research, and application. This model provides a valuable reference for talent cultivation and professional development in local universities.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"42 7","pages":"3335-3345"},"PeriodicalIF":0.0,"publicationDate":"2026-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148607445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Artificial intelligence-based mining of antimicrobial peptides in the microbiome].","authors":"Xiaoya Guo, Nannan Mo, Tengteng Fu, Yiqiong Lai, Xinyi Hou, Yunyan Zhou","doi":"10.13345/j.cjb.250842","DOIUrl":"10.13345/j.cjb.250842","url":null,"abstract":"<p><p>Antimicrobial peptides (AMPs) are small-molecule polypeptides with broad-spectrum antimicrobial activity that are induced by the innate immune system of organisms and constitute a crucial component of the innate immune defense. With the misuse of antibiotics and other antimicrobial agents, the problem of bacterial resistance has become increasingly severe. Naturally occurring AMPs derived from the microbiome, owing to their broad availability, stable physicochemical properties, relatively low propensity to induce resistance, and capacity to contribute to the maintenance of commensal microbiota homeostasis, are regarded as beneficial supplements and adjuvant therapeutic strategies to traditional antibiotics. In recent years, the rapid advancement of artificial intelligence (AI) technologies has facilitated their application in the field of drug discovery, thereby opening new avenues for the large-scale screening of AMPs and substantially accelerating the overall research progress. This review summarizes the developmental trajectory of AMP research methodologies from early approaches to the present day and provides a comparative overview of AI-based AMP screening tools and databases. In addition, the criteria for AMP screening and the recent progress in AI-assisted AMP development, both domestically and internationally, are systematically compiled. The aim of this review is to provide a systematic synthesis of the methodological framework for AI-based mining of microbiome-derived AMPs, thereby offering theoretical references and technical guidance for the efficient identification of novel AMPs. It is anticipated that this review will stimulate further consideration regarding AMP screening and design, and will promote advancements in the field of human health in the era of AI.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"42 7","pages":"2881-2901"},"PeriodicalIF":0.0,"publicationDate":"2026-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148607538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Construction and analysis of a deep-coverage proteomic map of <i>Mycobacterium avium</i> based on high-resolution mass spectrometry].","authors":"Yuwei Zhang, Songhao Jiang, Guilian Li, Jiaxing Dai, Tao Zhang, Lei Chang, Yao Zhang, Ping Xu","doi":"10.13345/j.cjb.260228","DOIUrl":"10.13345/j.cjb.260228","url":null,"abstract":"<p><p><i>Mycobacterium avium</i> is an important zoonotic nontuberculous mycobacteria (NTM), and its continuously rising infection rate has posed a severe public health challenge. However, due to the incomplete annotation of protein-coding sequences in the reference genome and the absence of proteomic data for the type strain, the expression profiles of the proteins encoded by this bacterium remain poorly characterized. To address the gap between genome prediction annotation and protein expression, it is crucial to acquire systematic and reliable experimental evidence of proteins at the type strain level. This study conducted an in-depth mass spectrometry analysis of whole-cell and the secreted proteins from the type strain <i>M</i>. <i>avium</i> DSM 44156^T. We employed a highly active acetylated trypsin developed by our laboratory for digestion, combined with high-pH reversed-phase fractionation. A total of 3 655 non-redundant proteins, 32 917 peptides, and 91 744 spectra were identified, covering 82.90% of the 4 409 coding genes in the NCBI reference protein database. The whole-cell and secretory proteomes exhibited pronounced complementarity, with a total of 2 445 proteins in common, while 1 114 and 96 unique proteins were specifically detected in the whole-cell and secreted fractions, respectively. This study has constructed a comprehensive and high-precision standardized proteomic map of <i>M</i>. <i>avium</i>, which not only supplements the protein expression data for this species but also provides key data support for further elucidating its pathogenic mechanism, screening novel diagnostic markers and therapeutic targets, and formulating prevention and control strategies for NTM-related zoonoses.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"42 7","pages":"3229-3242"},"PeriodicalIF":0.0,"publicationDate":"2026-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148607576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}