Sheng wu gong cheng xue bao = Chinese journal of biotechnology最新文献

{"title":"[Teacher team construction and leapfrog development pathways for bioengineering disciplines in industry-oriented universities].","authors":"Xiaole Xia, Jian Chen","doi":"10.13345/j.cjb.260068","DOIUrl":"10.13345/j.cjb.260068","url":null,"abstract":"<p><p>Building a high-level bioengineering discipline is a fundamental measure for promoting the development of the bioeconomy, and its advancement relies on the significant contributions of university faculty. Industry-oriented universities are inherently connected with both industries and academia, possessing unique advantages in serving industry needs, cultivating specialized talent, and supporting social development. Therefore, the question of how to achieve leapfrog development in bioengineering disciplines has imposed higher demands on the scientific research, teaching, and professional ethics of university faculty. This paper focused on the demands and contributions of the bioengineering discipline across related fields such as biomedicine, agriculture, food, environmental protection, resources, and chemical engineering. Progressing layer by layer from the core of discipline development, the responsibilities and requirements of faculty, the development elements of a \"good professor\", to the pathways for leapfrog development of the discipline, it systematically elucidated that bioengineering faculty should ground themselves on the discipline's characteristic of \"world-class and industry-grounded\", adhere to the principle of \"excelling in learning to serve as a teacher and upright in character to serve as a model\", and integrate their personal development into discipline construction and industry needs. \"Good professors\" advance teaching reform through outstanding teaching capabilities, academic accumulation, and collaborative service. On this basis, high-level faculty teams are formed around \"Good professors\", implementing a collaborative education model involving mentor groups that integrates industry, academia, and research. Centered on the integration of industry and education, scientific research achievements and industrial practice are transformed into teaching content to create a disciplinary feature that emphasizes teaching innovation and industrial empowerment. Through openness, collaboration, and resource integration, diverse practical platforms are established, thereby leading the leapfrog development of the discipline. This offers experience and recommendations for industry-oriented universities to advance the bioengineering discipline through faculty development.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"42 8","pages":"3789-3798"},"PeriodicalIF":0.0,"publicationDate":"2026-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148814141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Experimental and computational analyses of altered cetuximab binding induced by EGFR mutations].","authors":"Yifan Li, Haotong Yang, Jianwei Zhu, Mingyuan Wu","doi":"10.13345/j.cjb.260294","DOIUrl":"https://doi.org/10.13345/j.cjb.260294","url":null,"abstract":"<p><p>Acquired mutations in the extracellular domain of epidermal growth factor receptor (EGFR) are major causes of cetuximab resistance. However, the structural mechanisms by which different mutations impair antibody binding remain to be clarified. In this study, four clinically reported EGFR mutations, S492R, G465R, S464L, and I491M, were selected for investigation. Corresponding mutant EGFR fusion proteins were constructed, expressed, and purified. Enzyme-linked immunosorbent assay was performed to evaluate their binding ability to cetuximab. In parallel, SAAMBE-3D, FoldX, and Rosetta were employed to analyze mutation-induced changes in binding free energy, interfacial geometric complementarity, and local interaction patterns. The results showed that the half-maximal effective concentration (EC₅₀) of cetuximab binding to wild-type EGFR was 0.05 nmol/L. S492R, G465R, and S464L completely abolished cetuximab binding, whereas I491M only increased the EC₅₀ to 0.23 nmol/L, showing a weaker effect on antibody binding. Binding free energy analysis showed that all the four mutations reduced the stability of the EGFR-cetuximab complex, with I491M exerting a comparatively mild effect. Further analyses of interfacial geometric complementarity and structural features revealed that S492R and G465R mainly impaired interfacial matching by introducing marked steric hindrance and unfavorable repulsive effects, and S464L weakened antigen-antibody interactions by disrupting a critical hydrogen-bond network. I491M caused only mild perturbations in local hydrophobic interactions and conformational stability. In summary, by integrating <i>in vitro</i> binding assays with multiple structure-based computational methods, this study demonstrates that extracellular domain mutations of EGFR can attenuate cetuximab binding through enhanced steric hindrance, reduced interfacial complementarity, and disruption of key interaction networks. These findings provide experimental and theoretical evidence for understanding the structural basis of acquired cetuximab resistance and offer a reference for the rational design of antibodies targeting mutant EGFR.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"42 8","pages":"3643-3657"},"PeriodicalIF":0.0,"publicationDate":"2026-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148813690","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effects of soybean fermented bioactive peptides on intestinal health of bullfrogs].","authors":"Jianxiao Ren, Yuanqiang Lv, Jun Liao, Feifei Han, Daofeng Qu","doi":"10.13345/j.cjb.250873","DOIUrl":"https://doi.org/10.13345/j.cjb.250873","url":null,"abstract":"<p><p>To address the challenges of antibiotic overuse and high feed costs in bullfrog aquaculture, this study aimed to systematically evaluate the effects of soybean fermented bioactive peptides (SFP) as a functional feed additive on the growth performance and intestinal health of bullfrogs (<i>Lithobates catesbeianus</i>). A total of 450 healthy bullfrogs with an initial body weight of (100.0±2.0) g were randomly assigned into three groups: group C (basal diet), the 2% SFP group (basal diet supplemented with 2% SFP), and the 3% SFP group (basal diet supplemented with 3% SFP). The feeding trial lasted for 6 weeks. After the experiment, growth performance, intestinal morphology, digestive enzyme activities, relative mRNA expression levels of inflammatory cytokines, and expression levels of the tight junction protein ZO-1 were systematically measured. Additionally, the intestinal microbiota structure was analyzed using 16S rRNA gene high-throughput sequencing, and the content of short-chain fatty acids (SCFAs) in the intestine was determined by gas chromatography-mass spectrometry (GC-MS). The results showed that compared with the control group, dietary SFP supplementation significantly promoted bullfrog growth and improved intestinal health. Among the treatments, the 2% SFP group exhibited the most prominent effects, with significantly higher weight gain rate and specific growth rate than those in the control group and the 3% SFP group (<i>P</i>0.05). The 2% SFP supplementation also significantly increased the villus height in the jejunum and ileum (<i>P</i>0.05), enhanced intestinal digestive enzyme activities, remarkably downregulated the mRNA expression levels of intestinal pro-inflammatory cytokines (<i>P</i>0.05), and upregulated the expression of the tight junction protein ZO-1. Further analysis of the intestinal microbiota revealed that 2% SFP optimized the microbial community structure by increasing the relative abundance of beneficial bacteria such as <i>Clostridium</i> and <i>Lactococcus</i>. Although the 3% SFP group exhibited an increased relative abundance of <i>Bacteroides</i>, its modulatory effect on the intestinal microbiota structure was inferior to that of the 2% SFP group. Consequently, the intestinal content of acetic acid and n-butyric acid was significantly higher in the 2% SFP group than in the 3% SFP group (<i>P</i>0.05). Therefore, these findings demonstrate that dietary supplementation with 2% SFP enhances the growth performance and intestinal health of bullfrogs by optimizing the intestinal microbiota composition, strengthening intestinal metabolic capacity, improving intestinal barrier integrity, and facilitating better digestion and absorption. This study establishes an effective feeding strategy for feed additives to promote healthy bullfrog cultivation, thereby facilitating the green and sustainable development of the bullfrog aquaculture industry.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"42 8","pages":"3608-3623"},"PeriodicalIF":0.0,"publicationDate":"2026-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148813711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Extracellular metabolome remodeling mediates CART-suppressed bovine follicular development].","authors":"Junli Cheng, Jun Han, Meng Liu, Guangwen Zhang, Zhiwei Zhu, Pengfei Li","doi":"10.13345/j.cjb.260316","DOIUrl":"https://doi.org/10.13345/j.cjb.260316","url":null,"abstract":"<p><p>This study aimed to investigate the effects of cocaine- and amphetamine-regulated transcript peptide (CART) on the secretory function of bovine ovarian granulosa cells (GCs) and the metabolic micro-environment of follicular fluid. We employed untargeted metabolomics to analyze the metabolite profile of GC culture medium after treatment with CART_55-102, and integrated these data with transcriptomic and intracellular metabolomic data from the same experimental model. In the positive and negative ion modes, we identified 77 and 193 differential metabolites, respectively. Among these, we observed a significant decrease in glucose concentration and a significant increase in cholesterol concentration in the culture medium. Joint pathway enrichment analysis revealed that 6 and 17 Kyoto encyclopedia of genes and genomes (KEGG) pathways were commonly enriched by both differential genes and differential metabolites in the positive and negative ion modes, respectively. KEGG markup language (KGML) network analysis showed direct associations of <i>LRRC51</i> with cholesterol and 16α-hydroxyestrone in the steroid biosynthesis pathway, <i>ATP2B2</i> and <i>CALML4</i> with cholesterol in the aldosterone synthesis and secretion pathway, and <i>UROC1</i> with thiourocanic acid in the histidine metabolism pathway. On the basis of our previous intracellular metabolomic data (increased intracellular glucose and decreased intracellular cholesterol), the results of this study indicate that CART promotes glucose uptake by GCs but inhibits its intracellular oxidative utilization, leading to an increase in intracellular glucose content. At the same time, CART suppresses <i>de novo</i> cholesterol synthesis and promotes cholesterol release into the extracellular space, resulting in elevated extracellular cholesterol levels. In conclusion, this study proposes a potential novel mechanism hypothesis for CART inhibition of follicular development from a metabolic secretion perspective. That is, CART may remodel GC energy metabolism and cholesterol homeostasis to reshape the follicular fluid microenvironment. Our findings provide a new theoretical basis for understanding the regulatory mechanisms of bovine follicular development and have important reference value for improving the reproductive efficiency of cows and promoting the sustainable development of the cattle industry.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"42 8","pages":"3593-3607"},"PeriodicalIF":0.0,"publicationDate":"2026-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148813751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Quantitative trait locus (QTL) mapping and candidate gene analysis of heat tolerance of rice at heading stage].","authors":"Qianyun Jin, Fei Deng, Chenglei Zhong, Zhenghao Fu, Rongwan Zhang, Sushu Yang, Jiangmin Xu, Yuexing Wang, Kongjian Wang, Yuchun Rao","doi":"10.13345/j.cjb.260229","DOIUrl":"https://doi.org/10.13345/j.cjb.260229","url":null,"abstract":"<p><p>Rice (<i>Oryza sativa</i> L.) is one of the major food crops in China, and high-temperature stress has become one of the key abiotic stressors threatening stable rice production and food security. Rice is most sensitive to high temperatures at the heading stage. Heat stress can lead to the accumulation of reactive oxygen species, damage to cell membranes, and a reduction in seed-setting rate, thereby seriously affecting rice yields and quality. To identify quantitative trait loci (QTLs) associated with heat tolerance during the panicle emergence stage of rice and to gain deeper insights into the genetic basis of heat tolerance, in this study, the <i>indica</i> rice cultivar 'HZ', the <i>japonica</i> cultivar 'Nekken2', and their 120 recombinant inbred lines (RILs) were used as experimental materials. The seed-setting rate and heat tolerance coefficient of the population at the heading stage were evaluated for three consecutive years from 2023 to 2025. On the basis of the previously constructed high-density genetic linkage map, QTLs associated with heat tolerance of rice at the heading stage were identified. A total of 43 QTLs related to seed-setting rate and heat tolerance coefficient were identified, among which the QTL <i>qSSRIII-12</i>.<i>2</i> showed the highest logarithm of odds (LOD) value of 4.51. Chromosome 5 were identified with the most QTLs (9). In addition, some QTLs were overlapped. According to the QTL mapping intervals, 12 candidate genes were screened out. qRT-PCR was performed to analyze the expression patterns of these candidate genes. The results showed that the expression levels of most genes were significantly different between the parental lines. Among them, <i>LOC_Os05g30480</i>, <i>LOC_Os05g31040</i>, <i>LOC_Os05g31062</i>, and <i>LOC_Os07g37580</i> were upregulated after heat stress, which suggested that these genes might play a positive role in rice heat tolerance. In contrast, the expression levels of <i>LOC_Os10g28000</i>, <i>LOC_Os10g30680</i>, <i>LOC_Os12g25660</i>, and <i>LOC_Os12g26030</i> decreased after heat stress, which indicated that these genes may negatively regulate heat tolerance in rice. The results of this study provide a theoretical basis and important genetic resources for the mining and functional verification of heat tolerance genes in rice at the heading stage.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"42 8","pages":"3624-3642"},"PeriodicalIF":0.0,"publicationDate":"2026-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148813916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Research progress on the role and mechanism of ketogenic diet in regulating gametogenesis].","authors":"Tongtong Zhang, Chaoyue Cao, Weijun Pang","doi":"10.13345/j.cjb.250934","DOIUrl":"https://doi.org/10.13345/j.cjb.250934","url":null,"abstract":"<p><p>The ketogenic diet (KD) is a specialized dietary regimen characterized by high fat, adequate protein and low carbohydrate content. In recent years, KD has garnered increasing attention in the field of reproductive health due to its metabolic regulatory effects. Gametogenesis is a key process in reproduction, and the maintenance of its homeostasis is essential for normal reproductive function. However, the regulatory role of KD in gametogenesis and the underlying mechanisms have not been systematically summarized, and the relevant research findings remain controversial. KD exerts certain protective effects on gametogenesis; nevertheless, its efficacy in modulating apoptosis and inflammation is limited, and its effects are influenced by basal metabolic status and dietary composition. This review focuses on the impacts of KD on gametogenesis, addressing both spermatogenesis and oogenesis. It summarizes recent advances regarding how KD influences gametogenesis through multiple pathways involving oxidative stress, inflammatory responses, hormone levels, cellular energy metabolism and autophagy. Meanwhile, it summarizes the potential mechanisms involved in these effects. In addition, this paper also discusses the limitations of current research and future research directions, aiming to provide a theoretical reference for the clinical application potential and risks of KD in regulating reproductive function, and to offer novel insights for the prevention and treatment of reproductive health-related diseases.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"42 8","pages":"3391-3402"},"PeriodicalIF":0.0,"publicationDate":"2026-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148814040","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Construction and practice of a talent training model for synthetic biology].","authors":"Chihe Sun, Ling Zhang, Xianzhong Chen, Long Liu, Guocheng DU","doi":"10.13345/j.cjb.260172","DOIUrl":"10.13345/j.cjb.260172","url":null,"abstract":"<p><p>Synthetic biotechnology is a key driver of green biomanufacturing and a strategic pillar for China in leading the new scientific revolution and gaining a competitive edge in cutting-edge technologies. The training of talents in synthetic biology aligns with higher education's mission to empower the development of new quality productive forces. This paper studied the distinctive characteristics of synthetic biology, including its strong interdisciplinary nature, high demand for engineering practice, and deep integration of digital and intelligent teaching methodologies. From a top-level design standpoint for the talent training, this paper summarized the innovative measures adopted by Jiangnan University in reforming its synthetic biology program and talent training model. After continuous exploration, the university has developed a curriculum module characterized by interdisciplinary integration, established a systematic engineering practice education system encompassing cognitive construction, competency advancement, and character development, and developed a digital platform to construct an intelligent ecosystem for education through science technology innovation competition. The resulting interdisciplinary, engineering-oriented, digitally-intelligent-driven training paradigm, along with the reform initiatives in areas such as training programs, curriculum systems, teaching models, practical training, and project competition, can serve as a reference for the development of related programs in other peer institutions, contributing to the training of innovative talents in synthetic biology.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"42 8","pages":"3777-3788"},"PeriodicalIF":0.0,"publicationDate":"2026-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148814117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Preparation and characterization of porcine IL-2 monoclonal antibodies].","authors":"Shengnan Han, Yang Yang, Yi Ru, Rongzeng Hao, Longhe Zhao, Bingzhou Lu, Yajun Li, Haoyang Cui, Bolu Sun, Haixue Zheng","doi":"10.13345/j.cjb.260061","DOIUrl":"https://doi.org/10.13345/j.cjb.260061","url":null,"abstract":"<p><p>Porcine interleukin-2 (pIL-2) is a key cytokine that coordinates cellular and humoral immune responses. To obtain active protein and highly specific tool antibodies for use in porcine immunological research and disease diagnosis, this study cloned the full-length pIL-2 gene into the pcDNA3.1 vector to construct the recombinant plasmid pcDNA3.1-pIL-2. The rpIL-2 was expressed and purified <i>via</i> the CHO eukaryotic expression system, and BALB/c mice were immunized with rpIL-2. Through cell fusion, indirect ELISA, and limiting dilution subcloning, two monoclonal antibodies with strong specificity and good reactivity against pIL-2 were selected, named 1C3 and 4G8, with subtypes IgG2b and IgG1 and antibody titers reaching 1:2 048 000 and 1:1 024 000, respectively. Western blotting and indirect immunofluorescence results confirmed that these two monoclonal antibodies had good reactivity with pIL-2. The successful expression of rpIL-2 and the preparation of highly specific monoclonal antibodies provide essential biological materials for subsequent research on porcine immune function and vaccine efficacy evaluation.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"42 8","pages":"3492-3502"},"PeriodicalIF":0.0,"publicationDate":"2026-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148813915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[A colloidal gold test strip assay for antibody detection based on the VP7 protein of epizootic hemorrhagic disease virus].","authors":"Mingxin Zhang, Xinbing Hu, Di Kang, Pengcheng Gao, Yunru Zhong, Zhancheng Tian, Guiquan Guan, Hong Yin, Jijun He, Youjun Shang, Xuerui Wan, Junzheng DU","doi":"10.13345/j.cjb.250940","DOIUrl":"https://doi.org/10.13345/j.cjb.250940","url":null,"abstract":"<p><p>To establish a rapid method for detecting antibodies against epizootic hemorrhagic disease virus (EHDV), the highly conserved group-specific protein VP7 was used as the target antigen in this study. The recombinant VP7 protein was expressed in Sf9 cells using a baculovirus expression system and subsequently purified. Polyclonal antibodies were generated by immunizing New Zealand white rabbits with the purified recombinant VP7 protein. Western blotting and cellular immunofluorescence assays confirmed the strong immunogenicity of the protein. A colloidal gold-based immunochromatographic test strip for detecting anti-EHDV antibodies was developed by conjugating recombinant streptococcal protein G with colloidal gold nanoparticles. The control line was coated with rabbit anti-streptococcal protein G antibody, while the test line was coated with the purified recombinant VP7 protein. Performance evaluation indicated that the test strip possessed desirable sensitivity, specificity, reproducibility, and stability. No cross-reactivity was observed with positive sera from animals infected with bluetongue virus, sheep pox virus, orf virus, peste des petits ruminants virus, foot-and-mouth disease virus, or lumpy skin disease virus. Testing of 200 clinical serum samples demonstrated a 97% coincidence rate between this test strip and a commercial competitive ELISA assay kit for EHDV antibody detection, with a Kappa value of 0.88. This study provides technical support for the rapid diagnosis of EHDV infection and contributes to disease surveillance and control.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"42 8","pages":"3711-3722"},"PeriodicalIF":0.0,"publicationDate":"2026-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148814075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Application of genetic engineering technology in heavy metal bioremediation].","authors":"Kaiyang Zheng, Yeting Weng, Ran Zhao","doi":"10.13345/j.cjb.260231","DOIUrl":"https://doi.org/10.13345/j.cjb.260231","url":null,"abstract":"<p><p>Conventional physical and chemical methods for addressing global heavy metal pollution are costly and prone to inducing secondary pollution. Bioremediation technologies, despite their ecological advantages, often exhibit limited efficacy due to the limited tolerance thresholds and bioaccumulation efficiencies of natural organisms. Consequently, the genetic engineering of natural organisms has emerged as a viable approach to enhancing heavy metal bioremediation capabilities. This paper systematically reviews the research progress and application mechanisms regarding the use of genetic engineering technologies to enhance the heavy metal remediation capabilities of microorganisms and plants. It focuses on the heterologous expression of functional genes <i>via</i> transgenic technology and explores the potential of gene editing technologies for heavy metal remediation, thereby offering a reference for future environmental remediation efforts and the development of related processes.</p>","PeriodicalId":21778,"journal":{"name":"Sheng wu gong cheng xue bao = Chinese journal of biotechnology","volume":"42 8","pages":"3437-3449"},"PeriodicalIF":0.0,"publicationDate":"2026-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148814080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}