Small Ruminant Research最新文献

{"title":"Obtaining and evaluating goat milk powder derived from milk of local producers in northwestern Argentina","authors":"Fernando Josué Villalva ,&nbsp;Ana Paula Olivares La Madrid ,&nbsp;Franco Darío Della Fontana ,&nbsp;Enzo Goncalvez de Oliveira ,&nbsp;Margarita Armada ,&nbsp;Adriana Ramón","doi":"10.1016/j.smallrumres.2025.107496","DOIUrl":"10.1016/j.smallrumres.2025.107496","url":null,"abstract":"<div><div>In Argentina, the production of fluid goat milk (GM) is an important economic and cultural activity, especially in Salta Province, where the production is artisanal. Diversifying this raw material into a powdered product is important because it increases its shelf life. In addition, its consumption is growing due to its nutritional and functional properties. This study aimed to obtain GM powder and evaluate its physicochemical, functional, and reconstitution characteristics. Pasteurized GM samples were processed at different total solids concentrations (GM1: 13 %; GM2: 25 %; GM3: 40 %; and GM4: 45 %). The samples were dehydrated by spray drying. Moisture, protein, fat, lactose, ash, acidity, water activity, color, bulk and tapped density, Carr and Hausner index, insolubility index, solubility, wettability, and dispersibility were analyzed. Data were statistically analyzed using ANOVA and multivariate analysis. GM3 and GM4 samples showed higher moisture, fat, ash, and acidity, as well as greater brightness and a tendency toward a yellow color. GM1 and GM2 samples exhibited unacceptable functional and reconstitution properties. The results showed that under certain drying conditions, the solid concentration significantly influenced the properties of powdered milk. The powdered goat milk obtained by spray drying showed variability in its properties depending on the concentration of total solids and drying conditions. Samples with higher solids concentrations exhibited better physicochemical and functional characteristics. This study provides a basis for optimizing spray drying conditions and improving the quality of powdered goat milk, offering a nutritionally valuable and marketable alternative in the region.</div></div>","PeriodicalId":21758,"journal":{"name":"Small Ruminant Research","volume":"247 ","pages":"Article 107496"},"PeriodicalIF":1.6,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143792253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Transcriptional abundance and cyto-protective action of class I small heat shock proteins in heat stressed caprine cardiac cells","authors":"S. Patnaik ,&nbsp;S.R. Mishra ,&nbsp;P.P. Satapathy ,&nbsp;S.S. Behera ,&nbsp;C. Mishra ,&nbsp;R.C. Patra ,&nbsp;A.K. Kundu","doi":"10.1016/j.smallrumres.2025.107497","DOIUrl":"10.1016/j.smallrumres.2025.107497","url":null,"abstract":"<div><div>The present study was aimed to document the transcriptional abundance and cyto-protective action of class I small heat shock proteins (HSPs) in heat stressed caprine cardiac cells. Cardiac cells were isolated from different heart tissues (n = 6) of goats and primary cardiac cells culture was done. Primary cardiac cells achieved 75–80 % confluence after 72 h of incubation. Further, confluent cardiac cells were exposed to heat stress at 42 °C for 0 (control), 20, 60, 100 and 200 min. Transcriptional abundance of HSPB1 did not change at 20 min, up-regulated (P &lt; 0.05) from 60 to 200 min and registered highest at 200 min of heat stress. Transcriptional abundance of HSPB5 and HSPB8 did not vary at 20 and 60 min and then significantly increased at 100 and 200 min with highest transcription at 200 min of heat stress. Transcriptional abundance of HSPB6 was elevated only at 200 min of heat stress. Transcriptional abundance of Caspase-3 was significantly decreased at 200 min of heat stress. Therefore, it is logical to speculate that differential transcriptional abundance of class I small HSPs at different durations of heat stress might maintain cardiac cell homeostasis during heat stress. Moreover, highest transcription of class I small HSPs might have down-regulated the transcriptional abundance of Caspase-3 at 200 min of heat stress suggesting their cyto-protective action on heat stressed caprine cardiac cells.</div></div>","PeriodicalId":21758,"journal":{"name":"Small Ruminant Research","volume":"247 ","pages":"Article 107497"},"PeriodicalIF":1.6,"publicationDate":"2025-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143799924","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Gas therapies improve chronic osteoarthritis and productivity in SRLV-infected dairy goats","authors":"Letícia Steves Dos Santos ,&nbsp;Lorena Jamila Alves Ferreira Guimarães ,&nbsp;Tatiane Pacheco Barenco De Oliveira ,&nbsp;Beatriz Souza Costa ,&nbsp;Raymundo Rizaldo Pinheiro ,&nbsp;Roberta Carvalho Basile ,&nbsp;Nathalie Costa Da Cunha ,&nbsp;Mário Felipe Alvarez Balaro","doi":"10.1016/j.smallrumres.2025.107489","DOIUrl":"10.1016/j.smallrumres.2025.107489","url":null,"abstract":"<div><div>Small ruminant lentiviruses (SRLV) are chronic and progressive diseases that compromise animal health and production. This study aimed to evaluate the ozone therapy on different clinical, production and ultrasound indices in dairy goats naturally infected by SRLV. A total of 42 adult Saanen goats with chronic signs of arthritis and weight loss were used after molecular and/or seropositivity to SRLV confirmation. Animals were allocated into three experimental groups: G_oxygen/ozone (n = 14); G_oxygen (n = 14); and G_control (n = 14). A therapeutic ozone dose of 0.05 mg/kg was adopted. Monthly, throughout 90 days, all goats underwent a clinical examination, carpal ultrasound and production index collection. There was a treatment effect for production (Body Condition Score [BCS] and body weight) and clinical (clinical articular index, biometric indices and carpal volumes) indices. G_oxygen/ozone showed a greater BCS when compared to G_control, and G_oxygen had a score equal to other groups (2.7 ± 0.4 ≠ 2.5 ± 0.3 = 2.6 ± 0.4 BCS). G_oxygen/ozone and G_oxygen had a greater body weight when compared to G_control (62.0 ± 9.9 = 61.2 ± 11.3 ≠ 57.2 ± 9.5 Kg). In clinical evaluations, G_oxygen and G_oxygen/ozone obtained lower carpal joint indices when compared to G_control (8.2 ± 1.0 = 8.2 ± 1.3 ≠ 8.7 ± 1.0 cm). Regarding joint ultrasound data, G_oxygen/ozone and G_oxygen had lower indices when compared to G_control. The distance from the joint capsule to the subchondral space (8.7 ± 3.4 = 8.6 ± 3.2 ≠ 11.0 ± 4.3 mm), area (153.7 ± 68.1 = 156.2 ± 69.4 ≠ 239.0 ± 89.3 mm²) and perimeter (81.6 ± 15.7 = 81.1 ± 16.4 ≠ 101.7 ± 15.9 mm) of the carpal lesion are highlighted. Likewise, there was found interaction between treatment and time for ultrasound parameters of the distance from the joint capsule to the subchondral space, area and perimeter of the carpal lesion. In conclusion, both gas therapies, under the current conditions, can be safely used to improve joint clinical signs and production rates in dairy goats naturally infected by SRLV displaying chronic arthritis and weight loss.</div></div>","PeriodicalId":21758,"journal":{"name":"Small Ruminant Research","volume":"247 ","pages":"Article 107489"},"PeriodicalIF":1.6,"publicationDate":"2025-04-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143833796","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association analysis of single nucleotide polymorphisms of LMOD3 gene and feed efficiency in sheep","authors":"Lijuan He ,&nbsp;Weimin Wang ,&nbsp;Xiaojuan Wang ,&nbsp;Deyin Zhang ,&nbsp;Yukun Zhang ,&nbsp;Yuan Zhao ,&nbsp;Xiaolong Li ,&nbsp;Liming Zhao ,&nbsp;Dan Xu ,&nbsp;Xiaobin Yang ,&nbsp;Zongwu Ma ,&nbsp;Dewen Kong ,&nbsp;Qi Zhang ,&nbsp;Xiaoxue Zhang","doi":"10.1016/j.smallrumres.2025.107494","DOIUrl":"10.1016/j.smallrumres.2025.107494","url":null,"abstract":"<div><div>Feed efficiency is a crucial target traits in livestock production. The <em>LMOD3</em> gene is involved in the assembly and stability regulation of actin fibers in muscle cells.The member related to the formation of actin filaments A is significantly associated with the high feed efficiency group. The idea of this paper was to explore the influence of the <em>LMOD3</em> gene polymorphism on the feed efficiency of Hu sheep. In this investigation, 984 male Hu sheep aged 120–140 days were meticulously evaluated for key parameters including body weight (BW), feed intake (FI), and feed conversion ratio (FCR). The polymorphic locus of <em>LMOD3</em> gene was identified by sanger sequencing. The expression level of the <em>LMOD3</em> was measured by reverse transcriptional quantitative polymerase chain reaction (qRT-PCR). The results showed that <em>LMOD3</em> was mutated from C to A at the 33976909 base of intron 1. The results of association analysis showed that the FCR of AA genotype was significantly lower than that of CC genotype (<em>P</em> &lt; 0.05), and the ADG of AA genotype was significantly higher than that of CC genotype (<em>P</em> &lt; 0.05). qRT-PCR results revealed that the mRNA expression of <em>LMOD3</em> in muscle was significantly higher than that in other tissues, except spleen (<em>P</em> &lt; 0.05). The expression levels of different genotypes in muscle tissue showed that AA genotype was significantly higher than CC genotype. In short, <em>LMOD3</em> may potentially regulate the feed efficiency of Hu sheep. The findings of this research have established a theoretical framework for understanding the growth and development of Hu sheep.</div></div>","PeriodicalId":21758,"journal":{"name":"Small Ruminant Research","volume":"247 ","pages":"Article 107494"},"PeriodicalIF":1.6,"publicationDate":"2025-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143759313","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"X-linked signatures of positive selection and adaptive genetic plasticity shaping high-altitude resilience in Indian Changthangi sheep","authors":"Rangasai Chandra Goli ,&nbsp;Karan Mahar ,&nbsp;Kiyevi G. Chishi ,&nbsp;Subrata Koloi ,&nbsp;Tanuj Gunturu ,&nbsp;Vikas Diwakar ,&nbsp;Muralidhar Metta ,&nbsp;Amit Kumar ,&nbsp;Ganesh N. Aderao ,&nbsp;KC Nikhil ,&nbsp;Soumajit Sarkar ,&nbsp;Nidhi Sukhija ,&nbsp;KK Kanaka","doi":"10.1016/j.smallrumres.2025.107492","DOIUrl":"10.1016/j.smallrumres.2025.107492","url":null,"abstract":"<div><div>Recent genomic studies have focused on understanding the genetic basis of high-altitude adaptation, a classic example of evolutionary response to extreme environments. While most of these studies have concentrated on autosomal regions, the role of sex chromosomes, particularly the X chromosome, in this adaptation has been relatively unexplored. So, in this study, we performed an intensive scan of the X chromosome of Changthangi sheep as well as other Indian and exotic sheep to understand the indications of high-altitude adaptation by exploring population structure, genetic relationships, and signatures of selection on X-chromosome. Our findings reveal a close genetic affinity between Changthangi and Tibetan sheep, suggesting a shared evolutionary history that is similar to the findings of autosomal study. Additionally, we uncovered evidence of gene flow from Deccani and Australian Merino breeds into the Changthangi gene pool. Through a comprehensive analysis of selection signatures, we identified 14 candidate genes and several unannotated loci, out of which nine genes were potentially associated with high-altitude adaptation and these genes are implicated in various biological processes, including fatty acid metabolism, muscle function, potassium channel regulation, transcription, glycogen metabolism, neuronal function, RNA modification, mRNA splicing, synaptic transmission, and hypoxia response. Our study was the first to provide valuable insights into X chromosome genetic mechanisms underlying high-altitude adaptation in Changthangi sheep. These, along with autosomal findings have implications for future breeding strategies aimed at improving the resilience and productivity of Changthangi sheep in harsh environments.</div></div>","PeriodicalId":21758,"journal":{"name":"Small Ruminant Research","volume":"247 ","pages":"Article 107492"},"PeriodicalIF":1.6,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143768483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of genetic selection for increased production on fitness traits of small ruminants","authors":"Carina Visser","doi":"10.1016/j.smallrumres.2025.107491","DOIUrl":"10.1016/j.smallrumres.2025.107491","url":null,"abstract":"<div><div>Many sheep and goat breeds worldwide are subjected to high levels of selection emphasis to increase production of meat, milk and fibres. The continuous selection on a small number of traits have resulted in unintended consequences, mostly as adverse effects on the fitness of animals. Selection for increased dairy production led to an increase in mastitis incidence, while selection for increased meat yield impacted on the prevalence of <em>MSTN</em> and <em>CLPG</em> mutations. Reproduction efficiency is the single most important trait in any small ruminant production system. Selection for increased litter sizes in small ruminants has resulted in increased numbers of triplet and quadruplet pregnancies, with an associated increase in mortalities and reproductive wastage. To optimize a genetic response, a balanced approach should be followed to set breeding objectives that include some of these fitness traits. Selection for increased resilience to even one stressor (such as mastitis) could result in an improvement of overall robustness. As the accurate recording of health and welfare traits is currently a limitation, mitigation strategies should include the generation of novel phenotypes which could also be included in genomic solutions to address the current shortcomings of breeding programs.</div></div>","PeriodicalId":21758,"journal":{"name":"Small Ruminant Research","volume":"247 ","pages":"Article 107491"},"PeriodicalIF":1.6,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143715154","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ruminal and intestinal morphometric parameters of lambs fed diets containing Mimosa tenuiflora (Willd.) hay replacing Buffel grass hay","authors":"Mikael Leandro Duarte de Lima Tolentino ,&nbsp;Laura Honório de Oliveira Tolentino ,&nbsp;Larissa Maria Feitosa Gonçalves ,&nbsp;Otávio Brilhante de Sousa ,&nbsp;José Morais Pereira Filho ,&nbsp;Tairon Pannunzio Dias-Silva ,&nbsp;Antonio Leandro Chaves Gurgel ,&nbsp;Marcos Jácome de Araújo","doi":"10.1016/j.smallrumres.2025.107493","DOIUrl":"10.1016/j.smallrumres.2025.107493","url":null,"abstract":"<div><div>This study aimed to evaluate the morphometric parameters of the ruminal papillae, and intestinal villi of lambs fed diets containing <em>Mimosa tenuiflora</em> (Willd.) hay replacing Buffel grass (<em>Cenchrus ciliaris</em> L.) hay. Twenty-eight Santa Inês male lambs with initial body weight of 20.3 ± 1.49 kg were used, being distributed in a completely randomized design with four experimental treatments (0, 333, 670, and 1000 g/kg of <em>Mimosa tenuiflora</em> hay). The width of the ruminal papillae was not affected (P &gt; 0.05), while their area decreased linearly (P = 0.01) as the level of <em>M. tenuiflora</em> hay increased. In the duodenum, the height (P &lt; 0.0001) and surface area (P = 0.01) of the villi decreased linearly with increasing <em>M. tenuiflora</em> level, while the width (P = 0.0001) and area (P = 0.03) decreased quadratically, reaching minimum estimated values of 132.95 and 50,625.53 µm at 436 and 575 g/kg of <em>M. tenuiflora</em> hay, respectively, when used as a replacement for Buffel grass hay. There was a quadratic increase in the height of the jejunum villi (P &lt; 0.0001) with a maximum value of 623.22 µm at the level of 333 g/kg, while the width quadratically decreased with a minimum value of 143.33 µm at the level of 526 g/kg and the area linearly decreased (P = 0.02). The height of the ileum villi (P = 0.0003) as well as the depth of the crypts in all segments (P &lt; 0.001) linearly decreased. Therefore, it is suggested the substitution of <em>C. ciliaris</em> hay by <em>M. tenuiflora</em> hay up to 333 g/kg DM of the diet not affecting ruminal and intestinal morphometric parameters.</div></div>","PeriodicalId":21758,"journal":{"name":"Small Ruminant Research","volume":"247 ","pages":"Article 107493"},"PeriodicalIF":1.6,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143748505","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive survey of caseous lymphadenitis in sheep and goats flocks of Iran: Clinical, bacteriological, and molecular insights","authors":"Hossein Esmaeili ,&nbsp;Seyed Mehdi joghataei ,&nbsp;Ali Khanjari ,&nbsp;Fatemeh Haji Agha Khiyabani","doi":"10.1016/j.smallrumres.2025.107490","DOIUrl":"10.1016/j.smallrumres.2025.107490","url":null,"abstract":"<div><div>Caseous lymphadenitis (CLA) is a chronic and highly contagious disease in sheep and goats caused by <em>Corynebacterium pseudotuberculosis</em>, resulting in significant economic losses in the livestock industry. Despite its global prevalence, studies on CLA at the flock level in Iran remain limited. This study aimed to investigate the prevalence and epidemiology of CLA in sheep and goat herds across all 31 provinces of Iran using clinical, bacteriological, and molecular diagnostic methods to inform effective control and management strategies. This cross-sectional study, conducted from 2017 to 2022, investigated CLA prevalence in sheep and goat herds across all 31 provinces of Iran. Using a stratified sampling approach, 120 herds per province were selected and categorized by size into small (&lt;70 animals), medium (70–150 animals), and large (&gt;150 animals) groups, ensuring diverse representation. Clinical examinations and microbiological analyses identified cases, while multiplex PCR confirmed the presence of <em>C. pseudotuberculosis</em>. Statistical methods assessed associations between flock size, species, and age. The study revealed distinct prevalence patterns of CLA across Iranian provinces, with low prevalence (&lt;5 %) observed in over 30 % of flocks, moderate prevalence (5–10 %) in 20–50 %, and high prevalence (&gt;10 %) in less than 15 %. Clinical signs were primarily observed in the prescapular and submandibular lymph nodes, with lesions confirmed by bacteriological and molecular methods. <em>C. pseudotuberculosis</em> was the primary causative agent, with other secondary pathogens occasionally identified. Multiplex PCR successfully confirmed <em>C. pseudotuberculosis</em> in all positive samples. This study highlights the localized prevalence of CLA across Iran, identifying areas of high prevalence that necessitate urgent intervention. The findings underscore the importance of integrating effective vaccination strategies, biosecurity measures, and early detection protocols to manage CLA and reduce its economic impact. Future efforts should prioritize developing and implementing region-specific vaccines and preventive measures in Iranian herds.</div></div>","PeriodicalId":21758,"journal":{"name":"Small Ruminant Research","volume":"246 ","pages":"Article 107490"},"PeriodicalIF":1.6,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143725471","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic characterization and bottleneck analysis of Molai Adu and Pallai Adu goats from Southern India","authors":"M. Seevagan ,&nbsp;T. Ravimurugan ,&nbsp;S.M.K. Karthickeyan ,&nbsp;T. Thamil Vanan ,&nbsp;T. Anna ,&nbsp;R. Rajendran ,&nbsp;P. Gopu","doi":"10.1016/j.smallrumres.2025.107488","DOIUrl":"10.1016/j.smallrumres.2025.107488","url":null,"abstract":"<div><div>Genetic characterization and bottleneck analysis of Molai Adu and Pallai Adu goats from southern India were conducted using 25 microsatellite markers recommended by the FAO (2011). Molai Adu, the only naturally polled goat breed in India, showed a total of 202 alleles, with an average of 8.08 ± 0.435 alleles per locus, while Pallai Adu, known for their prolific nature, exhibited a higher count of 219 alleles, averaging 8.76 ± 0.581 alleles per locus. Both breeds demonstrated high genetic diversity, as evidenced by the elevated number of observed alleles, Polymorphism Information Content (PIC), and expected heterozygosity values. Out of the 25 loci analyzed, only three were in Hardy-Weinberg equilibrium, with the remaining 22 loci showing significant deviations in both goat populations. The study also revealed a genetic identity of 0.7720 between Pallai Adu and Molai Adu goats, corresponding to a genetic distance of 0.2588. Bottleneck analysis indicated no signs of recent population bottlenecks, as most loci displayed heterozygote excess and an L-shaped allele frequency distribution, suggesting stable genetic diversity. These findings underscore the genetic richness and adaptability of Molai Adu and Pallai Adu goats, highlighting their importance for conservation and sustainable breeding initiatives.</div></div>","PeriodicalId":21758,"journal":{"name":"Small Ruminant Research","volume":"247 ","pages":"Article 107488"},"PeriodicalIF":1.6,"publicationDate":"2025-03-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143715153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dose-response of Asparagopsis extract and diet quality effects enteric methane emissions for sheep fed high-forage diets","authors":"Charlotte Adam ,&nbsp;Frances C. Cowley ,&nbsp;Daniel Korir Sitienei ,&nbsp;Nigel Tomkins ,&nbsp;Lewis Kahn ,&nbsp;James Preston ,&nbsp;Emma Doyle","doi":"10.1016/j.smallrumres.2025.107487","DOIUrl":"10.1016/j.smallrumres.2025.107487","url":null,"abstract":"<div><div><em>Asparagopsis spp</em>. (Asparagopsis) receives considerable attention, due to the antimethanogenic properties it exhibits when included in diets of ruminant livestock. However, only one previous study has investigated the use of Asparagopsis in sheep <em>in vivo,</em> offered as part of a modified total mixed ration, reporting up to 80 % enteric methane (CH₄) abatement at an inclusion of 3 % (organic-matter basis). The objective of this study was to investigate the interaction of bromoform (CHBr₃) dose and basal diet quality on CH₄ abatement in sheep, when fed a high forage basal diet with supplementary pellets containing CHBr₃ extracted from <em>Asparagopsis armata</em> in oil (Asp-oil). Sixty merino wethers were used in a split plot design of five supplementary pellet treatments (100 g/d) of increasing CHBr₃ concentrations (A0, 0 mg CHBr₃/kg DM; A1, 36 mg CHBr₃/kg DM; A2, 72 mg CHBr₃/kg DM; A3, 108 mg CHBr₃/kg DM; and A4, 144 mg CHBr₃/kg DM) and fed either a high-quality (neutral detergent fibre (NDF) 40 %) or low-quality (NDF 55 %) forage basal diet. Wethers were housed in individual pens for 117 days to allow for measurements of feed intake and production parameters. Five measurements of CH₄ production (MP, g CH₄/d), 14 days apart, were conducted with open circuit respiration chambers. Wool growth (g/d), intake of basal diet and supplementary pellets (kg DMI/d), and average daily gain (kg/d) were production parameters. There was no significant interaction between diet × Asp-oil level on CH₄ yield (MY, g CH₄/kg DMI), but independently each had a significant effect. In comparison to their respective control (A0), A4 resulted in a 12 % and 19 % MY abatement for high-quality and low-quality diets, respectively. Average daily gain was significantly affected by diet (P &lt; 0.001) but not Asp-oil inclusion. Wool growth rate was affected by diet (P = 0.05) only. This study has demonstrated that feeding sheep Asp-oil can reduced MP, regardless of diet quality. However, basal diet continues to influence MY. Future grazing trials are necessary to validate the results of this animal house experiment.</div></div>","PeriodicalId":21758,"journal":{"name":"Small Ruminant Research","volume":"246 ","pages":"Article 107487"},"PeriodicalIF":1.6,"publicationDate":"2025-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143696090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}