{"title":"印度西孟加拉邦山羊种群中流行蜱虫种类的鉴定","authors":"M. Rafiqul Amin , Sourabh Sulabh","doi":"10.1016/j.smallrumres.2025.107526","DOIUrl":null,"url":null,"abstract":"<div><div>Ticks are major ectoparasites of domestic animals, including small ruminants, and are responsible for the transmission of several tick-borne diseases. They can also damage hides, induce anemia, and cause tick paralysis. A study was conducted to investigate and identify ticks infesting goats from five districts in West Bengal, India. Ticks were collected from the bodies of goats in five districts of West Bengal. The two tick species that were collected were first identified based on morphological analysis as <em>Haemaphysalis bispinosa</em> and <em>Hyalomma kumari</em>. The mitochondrial 16S rRNA sequences of the ticks were also amplified using gene-specific primers. The lengths of the amplified 16S rRNA sequences of <em>H. bispinosa</em> and <em>H. kumari</em> ticks were 456 bp and 457 bp, respectively. The amplified fragments were sequenced and aligned with other sequences using NCBI BLAST. This further confirmed the results of the morphological analysis, as the two sequences were perfectly aligned with <em>the H. bispinosa</em> and <em>H. kumari</em> gene sequences. Phylogenetic analysis of gene sequences was performed using sequences similar to those found in the NCBI database. A regression model to determine the effects of age, sex, and season on the prevalence of tick infestation in goats was also tested on the collected data using Jamovi 2.5.3. Only two tick species, <em>Haemaphysalis bispinosa</em> and <em>Hyalomma kumari,</em> were found on the ears of goats, and infestation was significantly affected by variations in age and season.</div></div>","PeriodicalId":21758,"journal":{"name":"Small Ruminant Research","volume":"249 ","pages":"Article 107526"},"PeriodicalIF":1.6000,"publicationDate":"2025-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Identification of prevalent tick species in the goat population of West Bengal state of India\",\"authors\":\"M. Rafiqul Amin , Sourabh Sulabh\",\"doi\":\"10.1016/j.smallrumres.2025.107526\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Ticks are major ectoparasites of domestic animals, including small ruminants, and are responsible for the transmission of several tick-borne diseases. They can also damage hides, induce anemia, and cause tick paralysis. A study was conducted to investigate and identify ticks infesting goats from five districts in West Bengal, India. Ticks were collected from the bodies of goats in five districts of West Bengal. The two tick species that were collected were first identified based on morphological analysis as <em>Haemaphysalis bispinosa</em> and <em>Hyalomma kumari</em>. The mitochondrial 16S rRNA sequences of the ticks were also amplified using gene-specific primers. The lengths of the amplified 16S rRNA sequences of <em>H. bispinosa</em> and <em>H. kumari</em> ticks were 456 bp and 457 bp, respectively. The amplified fragments were sequenced and aligned with other sequences using NCBI BLAST. This further confirmed the results of the morphological analysis, as the two sequences were perfectly aligned with <em>the H. bispinosa</em> and <em>H. kumari</em> gene sequences. Phylogenetic analysis of gene sequences was performed using sequences similar to those found in the NCBI database. A regression model to determine the effects of age, sex, and season on the prevalence of tick infestation in goats was also tested on the collected data using Jamovi 2.5.3. Only two tick species, <em>Haemaphysalis bispinosa</em> and <em>Hyalomma kumari,</em> were found on the ears of goats, and infestation was significantly affected by variations in age and season.</div></div>\",\"PeriodicalId\":21758,\"journal\":{\"name\":\"Small Ruminant Research\",\"volume\":\"249 \",\"pages\":\"Article 107526\"},\"PeriodicalIF\":1.6000,\"publicationDate\":\"2025-05-08\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Small Ruminant Research\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0921448825000999\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"AGRICULTURE, DAIRY & ANIMAL SCIENCE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Small Ruminant Research","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0921448825000999","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}
Identification of prevalent tick species in the goat population of West Bengal state of India
Ticks are major ectoparasites of domestic animals, including small ruminants, and are responsible for the transmission of several tick-borne diseases. They can also damage hides, induce anemia, and cause tick paralysis. A study was conducted to investigate and identify ticks infesting goats from five districts in West Bengal, India. Ticks were collected from the bodies of goats in five districts of West Bengal. The two tick species that were collected were first identified based on morphological analysis as Haemaphysalis bispinosa and Hyalomma kumari. The mitochondrial 16S rRNA sequences of the ticks were also amplified using gene-specific primers. The lengths of the amplified 16S rRNA sequences of H. bispinosa and H. kumari ticks were 456 bp and 457 bp, respectively. The amplified fragments were sequenced and aligned with other sequences using NCBI BLAST. This further confirmed the results of the morphological analysis, as the two sequences were perfectly aligned with the H. bispinosa and H. kumari gene sequences. Phylogenetic analysis of gene sequences was performed using sequences similar to those found in the NCBI database. A regression model to determine the effects of age, sex, and season on the prevalence of tick infestation in goats was also tested on the collected data using Jamovi 2.5.3. Only two tick species, Haemaphysalis bispinosa and Hyalomma kumari, were found on the ears of goats, and infestation was significantly affected by variations in age and season.
期刊介绍:
Small Ruminant Research publishes original, basic and applied research articles, technical notes, and review articles on research relating to goats, sheep, deer, the New World camelids llama, alpaca, vicuna and guanaco, and the Old World camels.
Topics covered include nutrition, physiology, anatomy, genetics, microbiology, ethology, product technology, socio-economics, management, sustainability and environment, veterinary medicine and husbandry engineering.