基于线粒体d -环序列分析的保加利亚本地绵羊品种遗传多样性

IF 1.6 3区 农林科学 Q2 AGRICULTURE, DAIRY & ANIMAL SCIENCE
Georgi Radoslavov , Georgi Kalaydzhiev , Georgi Yordanov , Nadezhda Palova , Delka Salkova , Peter Hristov
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引用次数: 0

摘要

保加利亚的地理、地貌和气候特点特别有利于动物养殖,尤其是畜牧和绵羊养殖。这些条件为创造大约30种独特的地方绵羊品种创造了先决条件。在这项研究中,我们根据线粒体D-loop区域的一部分序列分析,调查了8种最受欢迎的保加利亚本地品种的遗传多样性。扩增几乎整个线粒体DNA (mtDNA) d环区(1180 bp)并测序。结果表明,该菌株存在192个单倍型,属于3个主要的单倍群。大多数样本显示欧洲单倍群B(89.6 %)的高患病率,其次是单倍群a(8.6 %)和C(1.8 %)。其他报道的线粒体单倍群均未观察到。不同单倍型数量、核苷酸数量和单倍型多样性均较高(分别为192、0.01286和0.996),表明所有群体存在多母源。所有研究群体的Tajima d检验值均为- 1.774 (p <; 0.05),表明罕见等位基因的丰度很可能是由于最近的瓶颈期后群体扩张所致。高水平的mtDNA多态性在被研究的当地绵羊品种表明,保加利亚独特的基因型的显著储备的存在,这是有待探索。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Genetic diversity of native Bulgarian sheep breeds based on the mitochondrial D-loop sequence analysis
The geographical, geomorphological, and climatic characteristics of Bulgaria are particularly favorable for animal breeding and, above all, for pastoral farming and sheep breeding. These conditions created prerequisites for the creation of about 30 unique local breeds of sheep. In this study we investigated the genetic diversity of eight of the most popular Bulgarian native breeds, based on the sequence analysis of a part of the mitochondrial D-loop region. An almost entire mitochondrial DNA (mtDNA) D-loop region (1180 bp) was amplified and sequenced. The obtained results showed the presence of a large number of haplotypes – 192, belonging to three main haplogroups. The majority of samples showed a high prevalence of the European haplogroup B (89.6 %), followed by haplogroup A (8.6 %) and C (1.8 %). None of the other reported mitochondrial haplogroups were observed. The number of different haplotypes, nucleotide and haplotype diversity was high (192, 0.01286, and 0.996, respectively), which is evidence for multiple maternal origins in all populations. The Tajima D-test value in all the study populations was −1.774 (p < 0.05), indicating that the abundance of rare alleles was most likely due to population expansion after a recent bottleneck. The high level of mtDNA polymorphism in the studied breeds of local sheep indicates the presence of a significant reserve of unique genotypes in Bulgaria, which is to be explored.
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来源期刊
Small Ruminant Research
Small Ruminant Research 农林科学-奶制品与动物科学
CiteScore
3.10
自引率
11.10%
发文量
210
审稿时长
12.5 weeks
期刊介绍: Small Ruminant Research publishes original, basic and applied research articles, technical notes, and review articles on research relating to goats, sheep, deer, the New World camelids llama, alpaca, vicuna and guanaco, and the Old World camels. Topics covered include nutrition, physiology, anatomy, genetics, microbiology, ethology, product technology, socio-economics, management, sustainability and environment, veterinary medicine and husbandry engineering.
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