Georgi Radoslavov , Georgi Kalaydzhiev , Georgi Yordanov , Nadezhda Palova , Delka Salkova , Peter Hristov
{"title":"基于线粒体d -环序列分析的保加利亚本地绵羊品种遗传多样性","authors":"Georgi Radoslavov , Georgi Kalaydzhiev , Georgi Yordanov , Nadezhda Palova , Delka Salkova , Peter Hristov","doi":"10.1016/j.smallrumres.2025.107527","DOIUrl":null,"url":null,"abstract":"<div><div>The geographical, geomorphological, and climatic characteristics of Bulgaria are particularly favorable for animal breeding and, above all, for pastoral farming and sheep breeding. These conditions created prerequisites for the creation of about 30 unique local breeds of sheep. In this study we investigated the genetic diversity of eight of the most popular Bulgarian native breeds, based on the sequence analysis of a part of the mitochondrial D-loop region. An almost entire mitochondrial DNA (mtDNA) D-loop region (1180 bp) was amplified and sequenced. The obtained results showed the presence of a large number of haplotypes – 192, belonging to three main haplogroups. The majority of samples showed a high prevalence of the European haplogroup B (89.6 %), followed by haplogroup A (8.6 %) and C (1.8 %). None of the other reported mitochondrial haplogroups were observed. The number of different haplotypes, nucleotide and haplotype diversity was high (192, 0.01286, and 0.996, respectively), which is evidence for multiple maternal origins in all populations. The Tajima D-test value in all the study populations was −1.774 (p < 0.05), indicating that the abundance of rare alleles was most likely due to population expansion after a recent bottleneck. The high level of mtDNA polymorphism in the studied breeds of local sheep indicates the presence of a significant reserve of unique genotypes in Bulgaria, which is to be explored.</div></div>","PeriodicalId":21758,"journal":{"name":"Small Ruminant Research","volume":"249 ","pages":"Article 107527"},"PeriodicalIF":1.6000,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Genetic diversity of native Bulgarian sheep breeds based on the mitochondrial D-loop sequence analysis\",\"authors\":\"Georgi Radoslavov , Georgi Kalaydzhiev , Georgi Yordanov , Nadezhda Palova , Delka Salkova , Peter Hristov\",\"doi\":\"10.1016/j.smallrumres.2025.107527\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>The geographical, geomorphological, and climatic characteristics of Bulgaria are particularly favorable for animal breeding and, above all, for pastoral farming and sheep breeding. These conditions created prerequisites for the creation of about 30 unique local breeds of sheep. In this study we investigated the genetic diversity of eight of the most popular Bulgarian native breeds, based on the sequence analysis of a part of the mitochondrial D-loop region. An almost entire mitochondrial DNA (mtDNA) D-loop region (1180 bp) was amplified and sequenced. The obtained results showed the presence of a large number of haplotypes – 192, belonging to three main haplogroups. The majority of samples showed a high prevalence of the European haplogroup B (89.6 %), followed by haplogroup A (8.6 %) and C (1.8 %). None of the other reported mitochondrial haplogroups were observed. The number of different haplotypes, nucleotide and haplotype diversity was high (192, 0.01286, and 0.996, respectively), which is evidence for multiple maternal origins in all populations. The Tajima D-test value in all the study populations was −1.774 (p < 0.05), indicating that the abundance of rare alleles was most likely due to population expansion after a recent bottleneck. The high level of mtDNA polymorphism in the studied breeds of local sheep indicates the presence of a significant reserve of unique genotypes in Bulgaria, which is to be explored.</div></div>\",\"PeriodicalId\":21758,\"journal\":{\"name\":\"Small Ruminant Research\",\"volume\":\"249 \",\"pages\":\"Article 107527\"},\"PeriodicalIF\":1.6000,\"publicationDate\":\"2025-05-09\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Small Ruminant Research\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0921448825001002\",\"RegionNum\":3,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"AGRICULTURE, DAIRY & ANIMAL SCIENCE\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Small Ruminant Research","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0921448825001002","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"AGRICULTURE, DAIRY & ANIMAL SCIENCE","Score":null,"Total":0}
Genetic diversity of native Bulgarian sheep breeds based on the mitochondrial D-loop sequence analysis
The geographical, geomorphological, and climatic characteristics of Bulgaria are particularly favorable for animal breeding and, above all, for pastoral farming and sheep breeding. These conditions created prerequisites for the creation of about 30 unique local breeds of sheep. In this study we investigated the genetic diversity of eight of the most popular Bulgarian native breeds, based on the sequence analysis of a part of the mitochondrial D-loop region. An almost entire mitochondrial DNA (mtDNA) D-loop region (1180 bp) was amplified and sequenced. The obtained results showed the presence of a large number of haplotypes – 192, belonging to three main haplogroups. The majority of samples showed a high prevalence of the European haplogroup B (89.6 %), followed by haplogroup A (8.6 %) and C (1.8 %). None of the other reported mitochondrial haplogroups were observed. The number of different haplotypes, nucleotide and haplotype diversity was high (192, 0.01286, and 0.996, respectively), which is evidence for multiple maternal origins in all populations. The Tajima D-test value in all the study populations was −1.774 (p < 0.05), indicating that the abundance of rare alleles was most likely due to population expansion after a recent bottleneck. The high level of mtDNA polymorphism in the studied breeds of local sheep indicates the presence of a significant reserve of unique genotypes in Bulgaria, which is to be explored.
期刊介绍:
Small Ruminant Research publishes original, basic and applied research articles, technical notes, and review articles on research relating to goats, sheep, deer, the New World camelids llama, alpaca, vicuna and guanaco, and the Old World camels.
Topics covered include nutrition, physiology, anatomy, genetics, microbiology, ethology, product technology, socio-economics, management, sustainability and environment, veterinary medicine and husbandry engineering.