Small Ruminant Research最新文献

{"title":"Polymorphisms of PRKAA1 and FABP4 genes and their association with feed efficiency in Hu sheep","authors":"Mengru Pu ,&nbsp;Deyin Zhang ,&nbsp;Liming Zhao ,&nbsp;Dan Xu ,&nbsp;Zongwu Ma ,&nbsp;Kunchao Han ,&nbsp;Lijuan He ,&nbsp;Chengqi Yan ,&nbsp;Qi Zhang ,&nbsp;Lianjun Feng ,&nbsp;Ziyue Xiao ,&nbsp;Lei Gao ,&nbsp;Peiliang Cao ,&nbsp;Guoxing Jia ,&nbsp;Dewen Kong ,&nbsp;Linting Li ,&nbsp;Jian Zhang ,&nbsp;Hongjian Li ,&nbsp;Weimin Wang ,&nbsp;Ping Gong ,&nbsp;Huibin Tian","doi":"10.1016/j.smallrumres.2024.107377","DOIUrl":"10.1016/j.smallrumres.2024.107377","url":null,"abstract":"<div><div>Feed efficiency (FE) is an important economic trait in the sheep industry, influenced by biological processes such as digestion and metabolism. <em>PRKAA1</em> gene participates in the synthesis and oxidation of fatty acids; the <em>FABP4</em> gene is closely associated with intracellular fatty acid regulation. In this study, we use Sanger sequencing, KASPar genotyping, and qRT-PCR to investigate the association between variations in <em>PRKAA1</em> and <em>FABP4</em> genes and feed efficiency in Hu sheep, the expression patterns of <em>PRKAA1</em> and <em>FABP4</em> genes in different tissues, as well as the differences in expression of different genotypes in tail fat. The association analysis results indicated that <em>PRKAA1</em> gene g.35431965 C &gt; T locus and <em>FABP4</em> gene g.62829807 C &gt; T locus were significantly associated with the feed conversion ratio (FCR) of Hu sheep aged 80–180 days, with the dominant genotypes being CC and CC, respectively. Simultaneously, there existed notable disparities among various genotype combinations. The qRT-PCR results showed <em>PRKAA1</em> and <em>FABP4</em> genes were widely expressed in ten tissues, of which the <em>PRKAA1</em> gene was lower expression level in rumen and lung, <em>FABP4</em> gene was significantly highest expression in the tail fat than the other tissues; the expression of two different genotypes in tail fat also exhibits significant differences (<em>P</em> ≤ 0.05). Therefore, these findings suggested that the variation of <em>PRKAA1</em> and <em>FABP4</em> genes might be used as novel genetic marker for improving feed efficiency in sheep.</div></div>","PeriodicalId":21758,"journal":{"name":"Small Ruminant Research","volume":"240 ","pages":"Article 107377"},"PeriodicalIF":1.6,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142427978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasound assessment of biometric parameters of the head and brain and Doppler indices in cerebral arteries in sheep in the prenatal period","authors":"Barbara Błaszczyk ,&nbsp;Tomasz Stankiewicz ,&nbsp;Małgorzata A. Szewczuk ,&nbsp;Pavitra Chundekkad","doi":"10.1016/j.smallrumres.2024.107374","DOIUrl":"10.1016/j.smallrumres.2024.107374","url":null,"abstract":"<div><div>The aim of this study was to perform a biometric analysis of the head in sheep embryos/fetuses and to determine Doppler parameters in the middle cerebral artery and umbilical artery in 12 prenatal sheep. Using B-Mode ultrasound, the biparietal distance (BPD), occipital-nasal length (ONL), eye length and width, choroid plexus width, and turbinate complex width were determined. Peak systolic velocity (PSV), end diastolic velocity (EDV), PSV/EDV ratio, resistance index (RI) and pulsatility index (PI) were also determined. From the 26th to the 110th day of pregnancy, a progressive increase in BPD and ONL was observed (p&lt;0.01 and p&lt;0.05). The width of the choroid plexus increased until the 96th day of pregnancy (p&lt;0.01 and p&lt;0.05). The PSV in the MCA during the last period of pregnancy was higher than in the first period of pregnancy (p&lt;0.05). A similar trend was noted for EDV (p&lt;0.01). PSV/EDV, RI and PI at the end of pregnancy were the lowest. In the first period of pregnancy, EDV was higher in the MCA than in the UA (p&lt;0.01), but in subsequent periods these differences were reversed (p&lt;0.05, p&lt;0.01). In the first stage of pregnancy, RI and PI in the MCA were lower than in the UA (p&lt;0.01) but in subsequent periods of pregnancy these differences were reversed. The results indicate that several parameters that indicate changes in the heads of prenatal sheep can be detected ultrasonographically.</div></div>","PeriodicalId":21758,"journal":{"name":"Small Ruminant Research","volume":"240 ","pages":"Article 107374"},"PeriodicalIF":1.6,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142427468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantitative analysis of gametic imprinting effects on productive and reproductive performances of Markhoz goat","authors":"Khabat Kheirabadi","doi":"10.1016/j.smallrumres.2024.107373","DOIUrl":"10.1016/j.smallrumres.2024.107373","url":null,"abstract":"<div><div>In this study, in order to assess the relative importance of gametic imprinting effects for the genetic variation of growth and reproduction traits of Markhoz goat, a data set were obtained from a total of 4983 individuals on productive and reproductive traits of this breed (collected from 1993 to 2017) was analysed. The studied productive traits included body weight (BW) of Markhoz kid at birth and 4 (WW), 6 (W6), 9 (W9), and 12 (W12) months of age, average daily weight gains (g day⁻¹) in the first two growth phases (i.e. from birth to weaning, ADG_0–4; and from weaning to 6-month age, ADG_4–6) and their corresponding Kleiber ratios (i.e. KR_0–4 and KR_4–6). In addition, reproduction traits of interest were the liter size at birth (LSB) and at weaning (LSW), total litter weight at birth (TLWB) and at weaning (TLWW) per does kidding, litter mean weight per kid born (LMWKB) and per kid weaned (LMWKW). Likelihood ratio tests and Akaike’s Information criterion indicated that gametic imprinting effects were significant only for BW, W9 and KR_0–4. Both paternal and maternal gametic imprinting effects had a similar influence on these traits, accounting for between 8 % (W9) and 10 % (BW and KR_0–4) of the total phenotypic variance. For these traits the model with the second-best fit was models including paternal gametic imprinting effects, which indicates crucial paternal gametic imprinting effects in the analyzed traits in the production context. For parentally gametic influenced traits of Markhoz kid, including these effects significantly decreased the direct additive genetic variances (20–94 % depending on the trait) and these reduction were higher when paternal and maternal gametic variance evaluated together than when evaluated separately. Generally these results indicate that when gametic imprinting effects are of importance, but not accounted for, heritability estimates are biased upward and subsequently the realized efficiency of selection is reduced. It was concluded that for more precise estimates of genetic parameters, extracting of overly influential observations from the dataset is necessary.</div></div>","PeriodicalId":21758,"journal":{"name":"Small Ruminant Research","volume":"240 ","pages":"Article 107373"},"PeriodicalIF":1.6,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142358852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The use of PCR method for adulteration detection of goat dairy products manufactured by smallholders","authors":"Anna Dąbrowska","doi":"10.1016/j.smallrumres.2024.107372","DOIUrl":"10.1016/j.smallrumres.2024.107372","url":null,"abstract":"<div><div>The analysis of food adulteration is an important element of improving health safety. Both accidental and intentional adulteration of food, can cause serious health consequences for humans and can be considered as health hazard. In recent years, molecular biology techniques has been increasingly used in food analysis due to their high precision and the ability to obtain results in a short time, as well as the identification and quantitative determination of food adulterants. The samples tested in the study were goat's milk, yogurts, fresh and ripening cheeses produced by smallholders all over Poland. The analysis included DNA isolation, which was used as template for duplex PCR and detection of goat’s and cow’s mitochondrial DNA fragments. The obtained results indicate that the applied technique enabled the rapid identification of adulteration in analyzed goat’s milk products, among which in only one product out of thirty five the presence of undeclared cow’s milk was detected. This indicates good food safety standards applied on small-scale farms.</div></div>","PeriodicalId":21758,"journal":{"name":"Small Ruminant Research","volume":"240 ","pages":"Article 107372"},"PeriodicalIF":1.6,"publicationDate":"2024-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142320086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Short duration heatwaves increase body temperature and alter blood gas balance but may not cause oxidative stress and intestinal structure variations in lambs","authors":"Minghao Zhang ,&nbsp;Frank R. Dunshea ,&nbsp;Robyn D. Warner ,&nbsp;Kristy DiGiacomo ,&nbsp;Aleena Joy ,&nbsp;Archana Abhijith ,&nbsp;Pragna Prathap ,&nbsp;Ting Ma ,&nbsp;Surinder S. Chauhan","doi":"10.1016/j.smallrumres.2024.107367","DOIUrl":"10.1016/j.smallrumres.2024.107367","url":null,"abstract":"<div><div>This study investigated the impact of short duration heatwaves (HW) on the body temperature, blood gas, intestinal histology, and oxidative stress parameters of second cross lambs. Seventy-two second cross lambs [Poll Dorset × (Border Leicester × Merino)] were selected and exposed to either one, three or five days HW (28–38°C and 40–60 % relative humidity (RH)) or thermoneutral (TN; 18–21°C, 40–55 % RH) conditions in climate-controlled chambers. Lambs exposed to one to five days HW exhibited higher face, eye and ear temperature compared to animals exposed to equal duration under TN conditions. HW also had a significant impact on blood gas parameters which include higher blood pH, and lower CO₂, Ca²⁺, Na⁺, CHCO₃^- and Cl^-. However, HW lambs’ histology structure of the ileum and the GSSG: GSH of the ileum and <em>Longissimus thoracis</em> (LT) muscle were not influenced (<em>P</em> &gt; 0.05) by HW. LT muscle showed higher total antioxidant capacity (TAC) in HW conditions, but the TAC of intestine and blood had no difference between HW and TN groups. These results suggest that short-duration HW (one to five days) had a significant impact on lambs’ body temperature and blood parameters, but neither caused oxidative stress nor any changes in their intestinal structure.</div></div>","PeriodicalId":21758,"journal":{"name":"Small Ruminant Research","volume":"240 ","pages":"Article 107367"},"PeriodicalIF":1.6,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0921448824001731/pdfft?md5=3c5e0832346261c56b204f599c62c847&pid=1-s2.0-S0921448824001731-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142314338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring novel approaches for fertility and subfertility prediction in dromedary male camels (Camelus dromedarius)","authors":"L.O. Méar ,&nbsp;G. Gnemmi ,&nbsp;A. Echegaray ,&nbsp;J.A. Skidmore ,&nbsp;C. Malo","doi":"10.1016/j.smallrumres.2024.107371","DOIUrl":"10.1016/j.smallrumres.2024.107371","url":null,"abstract":"<div><div>Dromedary bulls exhibit particularly suboptimal semen quality. In this species, standard breeding soundness evaluation (BSE) often cannot catch the subtle difference between fertile and subfertile males. Therefore, the aim was to explore novel diagnostic tools to predict fertility and recognize subfertility in dromedary bulls. We explored the relationship between semen quality, fertility, echotexture parameters, accessory glands volume and total testosterone. Two sets of experiments were performed: <em>in vitro</em> (semen group) and <em>in vivo</em> (natural mating group). Each group was assessed by standard BSE: clinical examination, testis volume, ultrasound analysis of testicular parenchyma (TP) and epididymis. For the first time, images of epididymal head were obtained. Testis volume was not correlated to any parameters studied. Ultrasound TP showed especially low sensitivity (20 % <em>in vitro</em> and 14 % <em>in vivo</em>). The standard BSE was found unsuccessful in assessing subfertility. Therefore, animals were then submitted to advanced BSE: digital image analysis of TP, assessment of accessory glands (prostate (P) and bulbourethral (BB) glands) and serum total testosterone concentration. Upon analysis, significant correlations were found among some echotexture parameters (white pixels, mean grey levels, area and density of hypoechogenic areas) and percentage of viability, head defects, mitochondria potential and fertility. Only white pixels showed strong sensitivity (86 %) and specificity (100 %). BB volume correlated with ejaculate volume. Total testosterone concentration did not correlate with any parameters studied. In conclusion, advanced BSE (digital ultrasound image analysis and accessory glands volume) proved to be a useful tool for predicting semen quality and detecting subfertility in dromedary camels.</div></div>","PeriodicalId":21758,"journal":{"name":"Small Ruminant Research","volume":"240 ","pages":"Article 107371"},"PeriodicalIF":1.6,"publicationDate":"2024-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142322881","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biochemical and physicochemical characteristics and volatile profiles of Erzincan Tulum cheese coagulated by herb-fortified artisanal lamb rennet","authors":"Ayla Arslaner ,&nbsp;Özgenur Türkmen","doi":"10.1016/j.smallrumres.2024.107368","DOIUrl":"10.1016/j.smallrumres.2024.107368","url":null,"abstract":"<div><div>The characteristics of Erzincan Tulum cheese (ETC), an artisanal ewe-milk cheese and the first cheese with the designation of origin in Türkiye, are closely related to the geographical location of production and the human factors influencing every stage of the production process. This study aims to determine the influence of different coagulants (namely, herb-fortified artisanal lamb rennet (ALR), artisanal calf rennet (ACR), and commercial calf rennet (CCR)) on the biochemical, physicochemical characteristics, and volatile compounds of ETC, produced on the Heybeli plateau of the Erzincan province. Using artisanal rennet increased total solids, fat content, and acidity while decreasing pH and aw values. The highest PTA-SN (soluble nitrogen in 5 % phosphotungstic acid, w/v) and TCA-SN (soluble nitrogen in 12 % trichloroacetic acid w/w) values at the end of the ripening period (day 150 of storage) were observed in the ALR-Cheese (ALR-C). Furthermore, this cheese exhibited the highest rate of lipolysis (P &lt; 0.01). Electrophoretograms revealed that <em>αs-</em>1 and <em>β</em>-casein degradation rates were the most significant in ALR-C. Following the 30th day of storage, the highest counts of lactic acid bacteria (LAB) were found in ALR-C. The most rapid reduction of coliforms, <em>E. coli</em>, and yeast-mold was observed in this sample throughout the storage. Eighty-nine volatile compounds were identified in the samples. Particularly noteworthy, ALR-C exhibited the highest ratios of hexanoic, octanoic, and decanoic acids after 90 days of storage. Additionally, steroid compounds were exclusively detected in this sample. The findings of this research make a substantial contribution to the existing literature concerning rennet types and the aromatic profile of ETC. These aspects are crucial for further investigations to enhance the cheese's quality and safety.</div></div>","PeriodicalId":21758,"journal":{"name":"Small Ruminant Research","volume":"240 ","pages":"Article 107368"},"PeriodicalIF":1.6,"publicationDate":"2024-09-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142322818","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anticipating the potential distribution of Fasciola spp. in Gilan province of Iran: Insights from MaxEnt and climate change scenarios","authors":"Galia Modabbernia ,&nbsp;Behnam Meshgi ,&nbsp;Ahmad Ali Hanafi-Bojd","doi":"10.1016/j.smallrumres.2024.107370","DOIUrl":"10.1016/j.smallrumres.2024.107370","url":null,"abstract":"<div><div>Fasciolosis, a parasitic disease affecting humans and animals, is uniquely influenced by climatic and environmental factors. Gilan province in northern Iran is recognized as a high-endemic area for this parasite. This study aims to assess the prevalence of fasciolosis in Gilan province during the current period and forecast the distribution pattern of the parasite in future periods by analyzing climatic variables and identifying the most critical factors impacting <em>Fasciola</em>. To evaluate the present status of fasciolosis in Gilan, we collected 189 sheep fecal samples from different parts of the province and quantified eggs per gram of feces in each sample. Meteorological and environmental data were obtained and clipped to the study area. A total of 19 presence points were used to model the habitat suitability of <em>Fasciola</em> spp. through the maximum entropy (MaxEnt) algorithm, with jackknife analysis to determine variable importance. To project the potential distribution of <em>Fasciola</em> spp. in Gilan province under future scenarios, we employed MaxEnt using current (1970–2000) and projected climatic data based on three representative concentration pathway scenarios (RCP 2.6, RCP 4.5, and RCP 8.5) to predict habitat suitability in 2030, 2050, and 2070.</div><div>The results of this study indicate the proportion of <em>Fasciola</em> spp. infection was highest in Talesh (46.37 %) and Langarud (45.7 %), while Rudsar (0 %) and Shaft (16.25 %) exhibited the lowest infection rates in Gilan province. MaxEnt modeling highlighted the significance of bioclimatic variables, particularly those associated with vegetation and temperature, such as temperature seasonality (Bio4) and normalized difference vegetation index (NDVI). The ecological niche modeling illustrated that the highest potential distribution for <em>Fasciola</em> in Gilan province is concentrated in the north-western and central regions, exhibiting an 80–100 % potential. However, projections for the future indicate a decrease to less than 20 % suitability for most of the province under all three scenarios until 2070. This study provides valuable insights into the dynamic relationship between climatic variables and <em>Fasciola</em> distribution, enabling better preparedness and control strategies for this trematode in Gilan province and other regions with similar climates.</div></div>","PeriodicalId":21758,"journal":{"name":"Small Ruminant Research","volume":"240 ","pages":"Article 107370"},"PeriodicalIF":1.6,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142322819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic diversity and evolutionary significance of Cahi-DRB1 gene in Indian goat from semi-arid regions","authors":"S.P. Naik ,&nbsp;P.V. Nandedkar ,&nbsp;A. Gurao ,&nbsp;P.K. Rout ,&nbsp;M.K. Singh ,&nbsp;S. Sajid. Ali ,&nbsp;P.R. Suryawanshi ,&nbsp;P.B. Ghorpade ,&nbsp;M. Chitkara ,&nbsp;R.S. Kataria ,&nbsp;G.R. Gowane ,&nbsp;M.S. Dige","doi":"10.1016/j.smallrumres.2024.107365","DOIUrl":"10.1016/j.smallrumres.2024.107365","url":null,"abstract":"<div><div>Unlocking the genetic diversity of the Cahi-DRB1 gene in Indian goat breeds sheds light on their immune system's resilience. While extensive studies have elucidated MHC diversity in cattle and sheep, research on goats remains limited. Thus, this study aimed to investigate the genetic diversity of cahi<em>-DRB1</em> across five goat breeds in India, encompassing a total of 233 individual goats. Using a PCR sequence-based typing approach, DRB1 fragments (285 bp) were amplified and sequenced. The PCR products were purified and sequenced. Amino acid variability within breeds was evaluated using the Wu-Kabat index at peptide-binding pockets. Selection analysis included assessing positive selection through relative rates of nonsynonymous and synonymous mutations, Z-test, and Tajima's test of neutrality. The findings revealed notable heterozygosity across breeds (mean: 100 %), with nucleotide content averaging 22.51 % for A, 18.62 % for T, 24.33 % for C, and 34.35 % for G. The GC content was rich (59.26 %) across breeds for the DRB1 region. The mean distance for DRB1 was highest in Barbari (0.20) and lowest in Osmanabadi goat (0.12), with a higher rate of transversions observed. The Wu-Kabat index assessments underscored substantial genetic variation within the peptide binding sites (PBS) of DRB1, with specific residues (12, 14, 33, 58, and 87) showing polymorphism relevant to antigen presentation. Positive selection pressure was evident across all breeds for the DRB1 locus (dN&gt;dS), highlighting its evolutionary significance. The rich diversity of the DRB1 gene underscores the fitness of the population and the significance of this locus for future selection programs. These results highlight the highly polymorphic nature of the DRB1 locus in Indian goat breeds, emphasizing its crucial role in modulating immune responses. Consequently, our study offers valuable insights for developing targeted breeding strategies to enhance disease resistance in goats, thereby providing practical implications for sustainable livestock management.</div></div>","PeriodicalId":21758,"journal":{"name":"Small Ruminant Research","volume":"240 ","pages":"Article 107365"},"PeriodicalIF":1.6,"publicationDate":"2024-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142311299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating the impact of a novel GHRHR gene variant on growth traits in Damascus goats","authors":"Mervan Bayraktar ,&nbsp;Nazan Koluman ,&nbsp;Mohammed Baqur S. Al-Shuhaib","doi":"10.1016/j.smallrumres.2024.107366","DOIUrl":"10.1016/j.smallrumres.2024.107366","url":null,"abstract":"<div><p>This study investigates the genotype and allele frequencies of three novel SNP loci (c.4218 T&gt;G, c.2203 T&gt;C, and c.7966 A&gt;C) in the GHRHR gene within the Damascus goat breed and their associations with growth traits. The genotype frequencies for c.4218 T&gt;G were 0.65 (TT), 0.15 (TG), and 0.20 (GG), with allele frequencies of 0.73 (T) and 0.27 (G), showing a significant deviation from Hardy-Weinberg equilibrium (HWE). The c.2203 T&gt;C locus had genotype frequencies of 0.75 (TT) and 0.25 (CC), and the c.7966 A&gt;C locus had 0.81 (AA) and 0.19 (CC), both consistent with HWE. Association analysis revealed significant correlations between the c.4218 T&gt;G SNP and body weight and rump width, with the TT genotype showing the highest averages for both traits. The c.2203 T&gt;C SNP was significantly associated with body weight and chest width, with the TT genotype again showing higher averages. The c.7966 A&gt;C SNP was associated with chest depth and body length, with the AA genotype displaying lower averages for these traits. In silico predictions using multiple computational tools indicated that the identified missense SNP (p.31Ile&gt;Ser) is novel and likely deleterious to the GHRHR protein's function and stability. Molecular docking demonstrated that the wild-type GHRHR binds more effectively with G protein alpha compared to the mutated form, predicting a mechanism through which this variant reduces the interactions of GHRHR with its cognate proteins. These findings provide valuable insights into the genetic factors influencing growth traits in Damascus goats and highlight the potential impact of the p.31Ile&gt;Ser SNP on GHRHR functionality. This research underscores the importance of incorporating genetic markers in breeding programs to enhance growth traits and overall productivity.</p></div>","PeriodicalId":21758,"journal":{"name":"Small Ruminant Research","volume":"240 ","pages":"Article 107366"},"PeriodicalIF":1.6,"publicationDate":"2024-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142168852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}