Small Ruminant Research最新文献

筛选
英文 中文
Fiber characteristics of the dromedary camel in the Arabian Peninsula 阿拉伯半岛单峰骆驼的纤维特征
IF 1.8 3区 农林科学
Small Ruminant Research Pub Date : 2024-05-03 DOI: 10.1016/j.smallrumres.2024.107276
Kawther M. Akbar, Bader H. Alhajeri, Hasan Alhaddad
{"title":"Fiber characteristics of the dromedary camel in the Arabian Peninsula","authors":"Kawther M. Akbar,&nbsp;Bader H. Alhajeri,&nbsp;Hasan Alhaddad","doi":"10.1016/j.smallrumres.2024.107276","DOIUrl":"https://doi.org/10.1016/j.smallrumres.2024.107276","url":null,"abstract":"<div><p>Dromedary camel fibers have long been utilized to make clothes, tents, ropes, mats, and carpets, however, only a minority of these fibers are currently used to produce textiles. Dromedary fiber classification based on quality traits is essential to investigate its textile potential. Therefore, the objectives of this study were to (1) develop a classification protocol for dromedary camel fibers based on crimp (i.e., shape), (2) investigate the characteristics of each fiber class using length, diameter, and medulla (i.e., the innermost hair layer) and (3) determine which fiber class is most suitable for the textile industry. Fiber samples were obtained from the mid-torso of 32 Arabian Peninsula camels of different types. Five crimp categories were identified (straight, C-shaped, wavy, ringed, and kinky). Across all crimp categories, the average length ranged from 20.20 – 43.93 mm whereas the mean fiber diameter ranged from 29.74 – 72.11 μm. Percent medullation extended from 68% to 100% with noticeable variation between crimp categories. Overall, fiber traits significantly differed between the crimp categories. Kinky hair was particularly distinct from the other crimp categories. The kinky crimp category is the most suitable dromedary fiber for the textile industry because its fibers are long, fine, and has the lowest percentage of medullated fibers. Hence, we recommend that selective breeding programs and genetic studies focus on the production of dromedaries with kinky hair fibers.</p></div>","PeriodicalId":21758,"journal":{"name":"Small Ruminant Research","volume":"235 ","pages":"Article 107276"},"PeriodicalIF":1.8,"publicationDate":"2024-05-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140844117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Molecular detection of Plasmodium DNA in goats in Kulon Progo, Indonesia 印度尼西亚库隆普罗戈山羊疟原虫 DNA 的分子检测
IF 1.8 3区 农林科学
Small Ruminant Research Pub Date : 2024-05-01 DOI: 10.1016/j.smallrumres.2024.107275
Aan Awaludin , Raden Wisnu Nurcahyo , Dwi Priyowidodo , Yudhi Ratna Nugraheni , Joko Prastowo
{"title":"Molecular detection of Plasmodium DNA in goats in Kulon Progo, Indonesia","authors":"Aan Awaludin ,&nbsp;Raden Wisnu Nurcahyo ,&nbsp;Dwi Priyowidodo ,&nbsp;Yudhi Ratna Nugraheni ,&nbsp;Joko Prastowo","doi":"10.1016/j.smallrumres.2024.107275","DOIUrl":"https://doi.org/10.1016/j.smallrumres.2024.107275","url":null,"abstract":"<div><p>The <em>Plasmodium</em> parasites transmitted through the bites of infected <em>Anopheles</em> mosquitoes cause malaria in humans. Despite extensive research on the malaria parasite in ungulates, its occurrence in goats in Indonesia, particularly in the Kaligesing and Girimulyo border areas, has yet to be reported. This study aimed to investigate the presence of malaria parasites in goats from these regions. We collected 81 blood samples from goats of different ages and sexes. Blood samples were drawn from the jugular vein and preserved using EDTA tubes. We evaluated the overall clinical status of the goats by monitoring their rectal temperature and capillary refill time (CRT). Microscopic analysis was carried out by observing blood smears under a microscope, but the <em>Plasmodium</em> parasite was not detected. Next, we employed a molecular method to detect <em>Plasmodium</em> DNA using a nested polymerase chain reaction (PCR) method that targeted the cytochrome C oxidase subunit I (COI) gene. The results confirmed the presence of <em>Plasmodium</em> DNA in 5 out of the 81 samples tested. The obtained data were analyzed descriptively, and Wilson’s confidence interval method was used to calculate confidence limits for the sample proportions. This study provides the first molecular detection of previously undocumented malaria parasites in goats within the Girimulyo region of Kulon Progo, Yogyakarta, Indonesia. Identifying <em>Plasmodium</em> sp. using molecular techniques highlights the need for continued surveillance and management strategies to prevent the spread of malaria in animals and humans.</p></div>","PeriodicalId":21758,"journal":{"name":"Small Ruminant Research","volume":"235 ","pages":"Article 107275"},"PeriodicalIF":1.8,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140905388","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A randomized control trial investigating the effectiveness of a commercial pneumonia vaccine (Part II): Weaned lambs 调查商用肺炎疫苗有效性的随机对照试验(第二部分):断奶羔羊
IF 1.8 3区 农林科学
Small Ruminant Research Pub Date : 2024-05-01 DOI: 10.1016/j.smallrumres.2024.107269
M.D. Gardner , J. Van Donkersgoed , C.A. Bauman , M.T. Spinato
{"title":"A randomized control trial investigating the effectiveness of a commercial pneumonia vaccine (Part II): Weaned lambs","authors":"M.D. Gardner ,&nbsp;J. Van Donkersgoed ,&nbsp;C.A. Bauman ,&nbsp;M.T. Spinato","doi":"10.1016/j.smallrumres.2024.107269","DOIUrl":"10.1016/j.smallrumres.2024.107269","url":null,"abstract":"<div><p>The objective of this randomized controlled vaccine field trial was to determine the effectiveness of a commercial respiratory bacterin, Ovipast™ Plus, administered to feedlot lambs at weaning, approximately 8 wk of age, to improve animal health, growth performance, and carcass traits. Lambs were weaned, weighed, and sorted into groups by sex and the previous Ovipast™ Plus vaccination status of their mother, because this is stage 2 of that ongoing trial (Gardner et al., 2023). Lambs born to vaccinated ewes were vaccinated with the Ovipast™ Plus bacterin and revaccinated 3–5 wk later. Lambs born to unvaccinated ewes were not vaccinated. During the growing and finishing phases of the trial, vaccination did not reduce pneumonia treatment rates, crude or pneumonia specific mortality rates, or improve growth rates. Vaccinated lambs had a lower carcass fat cover (P &lt; 0.001) and a 1.33 times increased odds of yielding a Grade 1 carcass compared to unvaccinated lambs (P = 0.01). Vaccination reduced carcass fat cover and improved yield grades, but it had no beneficial effect on disease rates or growth performance, suggesting a limited economic benefit of vaccinating lambs post-weaning, which were borne from ewes vaccinated with Ovipast™ Plus during gestation.</p></div>","PeriodicalId":21758,"journal":{"name":"Small Ruminant Research","volume":"234 ","pages":"Article 107269"},"PeriodicalIF":1.8,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0921448824000750/pdfft?md5=d9d81c31efde80fcd2ffa07dbfb4a79e&pid=1-s2.0-S0921448824000750-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140756530","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Dietary inclusion of pistachio wastes (Pistacia vera L.) to fattening male goat kids’ feeding: Chemical-mineral compositions, in vitro ruminal fermentation, in vivo digestibility, hemato-biochemical profile, and growth performance 在育肥公山羊饲粮中添加开心果废弃物(Pistacia vera L.):化学矿物质成分、体外瘤胃发酵、体内消化率、血液生化指标和生长性能
IF 1.8 3区 农林科学
Small Ruminant Research Pub Date : 2024-04-25 DOI: 10.1016/j.smallrumres.2024.107274
Mohsen Kazemi , Reza Valizadeh , Abdelfattah Zeidan Mohamed Salem
{"title":"Dietary inclusion of pistachio wastes (Pistacia vera L.) to fattening male goat kids’ feeding: Chemical-mineral compositions, in vitro ruminal fermentation, in vivo digestibility, hemato-biochemical profile, and growth performance","authors":"Mohsen Kazemi ,&nbsp;Reza Valizadeh ,&nbsp;Abdelfattah Zeidan Mohamed Salem","doi":"10.1016/j.smallrumres.2024.107274","DOIUrl":"10.1016/j.smallrumres.2024.107274","url":null,"abstract":"<div><p>Pistachio (PW, <em>Pistacia vera</em> L.) wastes are a collection of favorite materials that are produced in the pistachio processing factories, in combination with its industry derivatives of clusters, soft hull, leaves, hard shell, and kernel. Two experiments were carried out; in the 1st one, the chemical and mineral compositions, <em>in vitro</em> ruminal-digestive fermentation activities, and buffering capacity parameters were determined for PW and its derivatives. For the 2nd experiment, the nutritional effects of an aluminosilicate (AS) and polyethylene glycol (PEG), at 10 g/kg dietary dry matter (DM), on PW-based diets were investigated. However, forty Mahabadi male goat kids [5 months, 22 ± 2.0 kg body weight (BW)] were randomly divided into the four experimental diets: control (a basal diet without PW); PW (replaced 40 % of control ingredients with PW); PW + PEG (PW diet + 10 g PEG/kg DM); and PW + AS (PW diet + 10 g AS/kg DM). Crude protein content, dry matter digestibility (DMD), gas production, and metabolizable energy were increased (<em>P</em> &lt; 0.001) in kernel rather than other treatments. Hard shell exhibited the lowest (<em>P</em> &lt; 0.001) DMD and/or organic matter digestibility (at 24 and 96 h), and highest (<em>P</em> &lt; 0.001) neutral detergent fiber, acid detergent fiber, and iron concentrations. In leaves, total phenolic and tannins, calcium, magnesium, and manganese had the highest (<em>P</em> &lt; 0.001) concentrations <em>versus</em> other PW derivatives. Soft hull has the greatest (<em>P</em> &lt; 0.001) acid-base buffering capacity among the evaluated derivatives. Dietary supplementation with 40 % PW decreased (<em>P</em> &lt; 0.001) dry matter intake, final BW, average daily gain, nutrient digestibility, ruminal total volatile fatty acids, propionate, and acetate, but with an increase (<em>P</em> &lt; 0.001) in those parameters in PW + PEG and PW + AS diets. Blood urea nitrogen and total protein decreased (<em>P</em> &lt; 0.05) by dietary PW. The inclusion of 40 % PW in the diet reduced the growth performance and diet nutritive value, but improved plasma antioxidant status (total antioxidant capacity: and malondialdehyde). Addition of PW in goat kids’ diets with PEG or AS improved the digestibility and ruminal fermentation activities and enhanced growth performance. In conclusion, it is recommended to use PW at the dietary level of 40 % in fattening goat kids, provided that it is accompanied by adding AS or PEG to the diet.</p></div>","PeriodicalId":21758,"journal":{"name":"Small Ruminant Research","volume":"235 ","pages":"Article 107274"},"PeriodicalIF":1.8,"publicationDate":"2024-04-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140794536","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Alterations in the gut microbial composition and diversity associated with diarrhea in neonatal Peruvian alpacas 与秘鲁新生羊驼腹泻有关的肠道微生物组成和多样性变化
IF 1.8 3区 农林科学
Small Ruminant Research Pub Date : 2024-04-22 DOI: 10.1016/j.smallrumres.2024.107273
Celso Zapata , Richard Estrada , Oscar Oros , Diana Sánchez , Jorge L. Maicelo , Carlos I. Arbizu , Pedro Coila
{"title":"Alterations in the gut microbial composition and diversity associated with diarrhea in neonatal Peruvian alpacas","authors":"Celso Zapata ,&nbsp;Richard Estrada ,&nbsp;Oscar Oros ,&nbsp;Diana Sánchez ,&nbsp;Jorge L. Maicelo ,&nbsp;Carlos I. Arbizu ,&nbsp;Pedro Coila","doi":"10.1016/j.smallrumres.2024.107273","DOIUrl":"10.1016/j.smallrumres.2024.107273","url":null,"abstract":"<div><p>Diarrhea in alpacas is a clinically significant condition and the primary cause of morbidity in neonatal Peruvian alpacas. This study aimed to correlate early diarrhea in crias of alpaca with changes in the microbiota community. A total of 19 alpacas (aged 1–2 months) were collected, including nine with a health condition and ten healthy ones. Fecal samples were obtained under sterile conditions and their DNA was extracted. Sequencing of the V3-V4 region of the 16S rRNA gene was conducted in the Illumina platform, followed by bioinformatics analysis. Reduced microbial diversity was evident in alpacas afflicted with diarrhea, delineating contrasting microbial compositions in comparison to their healthy counterparts. The study characterized the predominant bacterial classes and phyla within the gut microbiota, with Firmicutes, Verrucomicrobiota, and Bacteroidota collectively constituting approximately 80% of the total bacterial population. Substantial disparities in these microbial compositions were observed between the two groups, a variance that appeared to be influenced by both age and the health status of the alpacas. The bacterial class Verrucomicrobiae exhibited a significant presence within the group of alpacas suffering from illness. Furthermore, specific pathogenic species such as <em>Clostridium spiroforme</em>, <em>Blautia</em>, and <em>Bacteroides fragilis</em> were detected in significantly higher proportions among the afflicted alpacas. The functional diversity across the two groups was also found to be markedly different, a distinction that is graphically represented in a heat map illustrating the fifty principal differential KEGGs. This study provides valuable insights into the role of gut microbiota in alpaca health and may have implications for veterinary care and management.</p></div>","PeriodicalId":21758,"journal":{"name":"Small Ruminant Research","volume":"235 ","pages":"Article 107273"},"PeriodicalIF":1.8,"publicationDate":"2024-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0921448824000798/pdfft?md5=c677af8a18d6e1570afbe4e88332ecaa&pid=1-s2.0-S0921448824000798-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140792725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Isolation and characterization of tanninolytic bacteria from sheep rumen contents: Assessment of tannin degrading, fibrolytic and feed digestibility potential 从绵羊瘤胃内容物中分离单宁酸分解菌并确定其特征:单宁降解、纤维分解和饲料消化潜力评估
IF 1.8 3区 农林科学
Small Ruminant Research Pub Date : 2024-04-19 DOI: 10.1016/j.smallrumres.2024.107272
Srobana Sarkar , Pankaj Kumar Kumawat , Randhir Singh Bhatt , Artabandhu Sahoo
{"title":"Isolation and characterization of tanninolytic bacteria from sheep rumen contents: Assessment of tannin degrading, fibrolytic and feed digestibility potential","authors":"Srobana Sarkar ,&nbsp;Pankaj Kumar Kumawat ,&nbsp;Randhir Singh Bhatt ,&nbsp;Artabandhu Sahoo","doi":"10.1016/j.smallrumres.2024.107272","DOIUrl":"10.1016/j.smallrumres.2024.107272","url":null,"abstract":"<div><p>To counteract the negative effects of phytotannins, ruminants host a diverse community of bacteria that break down tannins. These bacteria not only possess tanninolytic activity but also have other functional relevance which has not been properly examined. Therefore, the present study was conducted to isolate and characterize tannin degrading bacteria (TDB) with possible fibrolytic activity from rumen of sheep fed tannin-rich diet. Based on the development of a clear zone on tannin-enriched agar plates, a total of 24 isolates were screened as tannin hydrolyzing bacteria. Majority of the isolates were gram positive cocci. The isolates could tolerate phenolic monomers such as ferulic acid, gallic acid, vanillic acid up to 30 mM and pyrogallol up to 10 mM however, the isolates failed to grow in presence of syringic acid, p-coumaric acid and p-hydroxybenzoic acid. Highest tannase activity was observed in isolate TDB23 while, lowest in isolates TDB2 and TDB5. In terms of fibrolytic activity, maximum endoglucanase, exoglucanase, and FPase activity was observed in isolate TDB9. <em>In vitro</em> digestibility of <em>Prosopis cineraria</em> leaves was considerably enhanced by inoculation of isolate TDB23 followed by TDB9. Therefore, the screened isolates demonstrated promising tannin and fibre degrading potential which can be further explored as direct-fed microbial in ruminants for effective utilization of tannin-rich fibrous feeds.</p></div>","PeriodicalId":21758,"journal":{"name":"Small Ruminant Research","volume":"235 ","pages":"Article 107272"},"PeriodicalIF":1.8,"publicationDate":"2024-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140795100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development and validation of an indirect ELISA for the serodiagnosis of contagious caprine pleuropneumonia in goats 开发和验证用于山羊传染性绵羊胸膜肺炎血清诊断的间接酶联免疫吸附测定法
IF 1.8 3区 农林科学
Small Ruminant Research Pub Date : 2024-04-17 DOI: 10.1016/j.smallrumres.2024.107271
Hayatullah Khan , Farhan Anwar Khan , Umer Sadique , Ijaz Ahmad , Muhammad Saeed , Faisal Ahmad , Faiz Ur Rehman , Hanif Ur Rahman
{"title":"Development and validation of an indirect ELISA for the serodiagnosis of contagious caprine pleuropneumonia in goats","authors":"Hayatullah Khan ,&nbsp;Farhan Anwar Khan ,&nbsp;Umer Sadique ,&nbsp;Ijaz Ahmad ,&nbsp;Muhammad Saeed ,&nbsp;Faisal Ahmad ,&nbsp;Faiz Ur Rehman ,&nbsp;Hanif Ur Rahman","doi":"10.1016/j.smallrumres.2024.107271","DOIUrl":"10.1016/j.smallrumres.2024.107271","url":null,"abstract":"<div><p><em>Mycoplasma capricolum</em> subsp. <em>capripneumoniae</em> (Mccp) is a dreadful pathogen of contagious caprine pleuropneumonia (CCPP). Mccp local strains have recently been isolated from naturally infected goats in northern areas of Pakistan. In this study, we established an in-house indirect ELISA (iELISA) based on whole-cell proteins (WCP) of local Mccp strains for the detection of anti-Mccp antibodies. Serum samples from naturally (n=500) and experimentally infected (n=20) and non-infected animals (n=100) were used for the establishment of iELISA. The specificity and sensitivity of the iELISA were determined using a commercially available competitive ELISA (cELISA) kit as a reference standard. The cut-off value (OD<sub>450</sub>) was found 0.53 by ROC analysis. As indicated by a kappa value of 0.88 and 96.66% agreement, both assays showed perfect agreement. An iELISA based on WCP of local Mccp detected seroconversion in experimental goats 7 days post-infection (dpi). The iELISA revealed a sensitivity of 90.83% (95% CI: 83.77%, 95.55%) and a specificity of 92.31% (95% CI: 84.79%, 96.85%) and has no cross-reaction with related mycoplasma epidemic diseases. For both inter-assay and intra-assay precision, the iELISA percentage coefficient of variation (%CV) was 4.8% and 1.74%, respectively. In conclusion, this in-house iELISA based on local Mccp-WCP was determined as a sensitive and specific assay for the diagnosis of Mccp infection.</p></div>","PeriodicalId":21758,"journal":{"name":"Small Ruminant Research","volume":"235 ","pages":"Article 107271"},"PeriodicalIF":1.8,"publicationDate":"2024-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140768595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Predicting fat deposition in growing Boer goat castrates and does using ultrasonography 利用超声波成像技术预测生长期波尔山羊阉羊和母羊的脂肪沉积情况
IF 1.8 3区 农林科学
Small Ruminant Research Pub Date : 2024-04-15 DOI: 10.1016/j.smallrumres.2024.107270
T.S. Brand , J.P. van der Westhuyzen , P.G. Theron , J.H.C. van Zyl
{"title":"Predicting fat deposition in growing Boer goat castrates and does using ultrasonography","authors":"T.S. Brand ,&nbsp;J.P. van der Westhuyzen ,&nbsp;P.G. Theron ,&nbsp;J.H.C. van Zyl","doi":"10.1016/j.smallrumres.2024.107270","DOIUrl":"https://doi.org/10.1016/j.smallrumres.2024.107270","url":null,"abstract":"<div><p>Ultrasonography was used to determine the subcutaneous backfat deposition of Boer goat castrates and does from weaning until one year of age. Logarithmic regressions of subcutaneous backfat measurements were constructed using body weight and age as inputs. The logarithmic regression of subcutaneous backfat deposition with bodyweight had a moderate to good fit, with Boer goat does displaying a better fit (R<sup>2</sup>=0.70) than castrates (R<sup>2</sup>=0.65). The logarithmic regression of subcutaneous backfat with age indicated a good fit for both sexes (R<sup>2</sup>=0.85). Castrates and does displayed similar rates of subcutaneous backfat deposition. The models proposed here will enable producers to estimate the subcutaneous backfat depth of Boer goat castrates and does with reasonable accuracy and can be utilized as benchmarks in precision Boer goat rearing applications, such as predicting an appropriate slaughter weight.</p></div>","PeriodicalId":21758,"journal":{"name":"Small Ruminant Research","volume":"234 ","pages":"Article 107270"},"PeriodicalIF":1.8,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0921448824000762/pdfft?md5=ed7b4fd526b86ee0d27243968cb3028b&pid=1-s2.0-S0921448824000762-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140606816","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A randomized control trial investigating the effectiveness of a commercial pneumonia vaccine (part I): Pre-weaned lambs 随机对照试验:调查商用肺炎疫苗的有效性(第一部分):断奶前羔羊
IF 1.8 3区 农林科学
Small Ruminant Research Pub Date : 2024-04-12 DOI: 10.1016/j.smallrumres.2024.107268
M.D. Gardner , J. Van Donkersgoed , C.A. Bauman , M.T. Spinato
{"title":"A randomized control trial investigating the effectiveness of a commercial pneumonia vaccine (part I): Pre-weaned lambs","authors":"M.D. Gardner ,&nbsp;J. Van Donkersgoed ,&nbsp;C.A. Bauman ,&nbsp;M.T. Spinato","doi":"10.1016/j.smallrumres.2024.107268","DOIUrl":"https://doi.org/10.1016/j.smallrumres.2024.107268","url":null,"abstract":"<div><p>The objective of this controlled vaccine field trial was to determine the effectiveness of a commercial bacterin in increasing colostral immunity to reduce the risk of bacterial pneumonia in pre-weaned lambs in a commercial sheep operation. Pregnant ewes were randomly allocated to vaccination group (Ovipast™ Plus bacterin, n = 1807; unvaccinated, n = 1812). Ewe vaccination did not significantly (P &gt; 0.05) improve lamb pneumonia treatment rates, crude or pneumonia specific mortality rates, or body weight gain. Interestingly, as birth weight increased in lambs from vaccinated ewes, they gained significantly more than lambs from unvaccinated ewes (P = 0.01). There was no difference in culture results from pneumonic lung samples for either <em>Mannheimia haemolytica</em> (P = 0.89) or <em>Bibersteinia trehalosi</em> (P = 1.00) between lambs from vaccinated and unvaccinated ewes. The results of this study suggest that there was no animal health and welfare benefit from vaccinating ewes with the Ovipast™ Plus bacterin prior to parturition to boost colostral immunity and improve health and growth in their lambs.</p></div>","PeriodicalId":21758,"journal":{"name":"Small Ruminant Research","volume":"234 ","pages":"Article 107268"},"PeriodicalIF":1.8,"publicationDate":"2024-04-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S0921448824000749/pdfft?md5=2d51f97856fb5f0f42c76d6eb54115b3&pid=1-s2.0-S0921448824000749-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140632963","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
The novel candidate gene Grb10 regulates the growth performance of Guizhou white goats 新型候选基因Grb10调控贵州白山羊的生长性能
IF 1.8 3区 农林科学
Small Ruminant Research Pub Date : 2024-04-10 DOI: 10.1016/j.smallrumres.2024.107267
Lingang Dai , Xiang Chen , Dongwei An , Meimei Xiao , Jiajing Huang , Jiali Xu , Yong Ruan
{"title":"The novel candidate gene Grb10 regulates the growth performance of Guizhou white goats","authors":"Lingang Dai ,&nbsp;Xiang Chen ,&nbsp;Dongwei An ,&nbsp;Meimei Xiao ,&nbsp;Jiajing Huang ,&nbsp;Jiali Xu ,&nbsp;Yong Ruan","doi":"10.1016/j.smallrumres.2024.107267","DOIUrl":"https://doi.org/10.1016/j.smallrumres.2024.107267","url":null,"abstract":"<div><p>Growth factor receptor binding protein 10 (<em>Grb10</em>) regulates muscle growth but its function in goats is not well studied. This study examined the relationship between <em>Grb10</em> gene polymorphism and growth traits in Guizhou white goats. Further, the effect of promoter polymorphism on <em>Grb10</em> transcription was analyzed via a dual-luciferase assay. Quantitative reverse transcription-polymerase chain reaction analysis showed that the expression level of the <em>Grb10</em> gene was the highest in the longissimus dorsi muscle (<em>P</em> &lt; 0.01) and the lowest in the spleen. Moreover, sequencing results showed that the <em>Grb10</em> gene had five single nucleotide polymorphisms. The association analysis showed that g.–246 C &gt; T, g.72323 T &gt; C, and g.178634 A &gt; G loci were significantly associated with growth traits (<em>P</em> &lt; 0.05). Linkage disequilibrium analysis of each locus showed that g.72323 T &gt; C and g.72814 T &gt; A loci had the strongest linkage (<em>r</em><sup>2</sup> = 0.482, <em>D'</em> = 0.73). Furthermore, according to an analysis conducted using TFDB4 software, it was predicted that a mutation in the promoter region of the g.–246 C &gt; T locus resulted in the absence of four transcriptional regulatory factors. The results of dual-luciferase reporter assay further demonstrated that mutation of the g.–246 C &gt; T locus reduced the transcriptional activity of the promoter region of the <em>Grb10</em> gene. These findings suggest that <em>Grb10</em> gene polymorphism could affect growth traits in goats.</p></div>","PeriodicalId":21758,"journal":{"name":"Small Ruminant Research","volume":"234 ","pages":"Article 107267"},"PeriodicalIF":1.8,"publicationDate":"2024-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140546242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信