Small Ruminant Research最新文献

{"title":"Effects of restricted- and over-feeding during gestation on colostrum and milk composition and offspring circulating immunoglobulin G concentrations in multiple generations of sheep","authors":"N.M. Tillquist ,&nbsp;M.Y. Kawaida ,&nbsp;A.S. Reiter ,&nbsp;V.V. Bassani ,&nbsp;J.M. Bosco ,&nbsp;A.B. Bettencourt ,&nbsp;R.J. Gifford ,&nbsp;T.E. Engle ,&nbsp;S.A. Zinn ,&nbsp;K.E. Govoni ,&nbsp;S.A. Reed","doi":"10.1016/j.smallrumres.2024.107423","DOIUrl":"10.1016/j.smallrumres.2024.107423","url":null,"abstract":"<div><div>High-quality colostrum and milk are critical for neonatal growth and immune system development. Immunoglobulin G (IgG), the predominant antibody in colostrum, is vital for passive immune transfer in sheep. It is unknown if poor maternal nutrition during gestation influences colostrum and milk composition, and IgG concentrations over multiple generations. We hypothesized that poor maternal nutrition (restricted- or over-feeding) during gestation would alter F0 and F1 colostrum and milk composition and IgG concentrations in their colostrum and alter serum IgG in F1 and F2 offspring. Multiparous Dorset ewes (n = 46) pregnant with twins were fed 100 % (control; n = 13), 60 % (restricted; n = 17) or 140 % (over; n = 16) of National Research Council (NRC) requirements until parturition. Following lambing, all dams were fed 100 % NRC requirements during lactation. Offspring of these ewes (n = 85; F1) were managed similarly on 100 % of NRC requirements and are referred to as CON-F1, RES-F1, or OVER-F1, corresponding with dam diet. F1 ewe offspring (n = 36) were bred between 16 and 19 mo of age. Offspring of these ewes (n = 60; F2) were managed similarly on 100 % of NRC requirements and are referred to as CON-F2, RES-F2, or OVER-F2, corresponding with granddam diet. Colostrum samples from F0 and F1 ewes were collected within 24 h of parturition and milk samples were collected at d 3 and 21 postpartum and analyzed for total solids, crude fat, crude protein (colostrum and milk), and IgG concentrations (colostrum only). Serum samples were collected from F1 and F2 offspring at 7 d of age and analyzed for IgG concentrations. A treatment by time interaction (<em>P</em> ≤ 0.024) was observed for F0 crude fat where colostrum from restricted-fed ewes had less fat than colostrum from control-fed ewes. However, total fat in d 3 and 21 milk samples were similar across treatments. Colostrum had greater total solids, crude fat, and crude protein compared with milk at d 3 and d 21 (<em>P</em> ≤ 0.01). Maternal diet had no impact on IgG concentrations in F0 or F1 ewe colostrum (<em>P</em> ≥ 0.46). A treatment by sex interaction (<em>P</em> = 0.007) was observed for F1 offspring serum IgG concentrations where RES-F1 and OVER-F1 ewes and CON-F1 and OVER-F1 rams had between 44.9 – 50.7 % less IgG than CON-F1 ewes. No impact of maternal diet was observed on F2 offspring serum concentration of IgG (<em>P</em> = 0.40). In this experiment, maternal diet had minimal effects on F0 and F1 colostrum and milk composition or IgG concentrations. However, maternal diet may limit IgG absorption in F1 offspring in a sex- and diet-specific manner but does not persist into the F2 generation.</div></div>","PeriodicalId":21758,"journal":{"name":"Small Ruminant Research","volume":"243 ","pages":"Article 107423"},"PeriodicalIF":1.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143161674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Global prevalence of Mycobacterium avium subsp. paratuberculosis in sheep: Systematic review and meta-analysis","authors":"Nathália Maria de Andrade Magalhães ,&nbsp;Clécio Henrique Limeira ,&nbsp;Rafael Dioni Leandro Costa ,&nbsp;Roseane de Araujo Portela ,&nbsp;Sérgio Santos de Azevedo ,&nbsp;Francisco Selmo Fernandes Alves ,&nbsp;Clebert José Alves","doi":"10.1016/j.smallrumres.2025.107430","DOIUrl":"10.1016/j.smallrumres.2025.107430","url":null,"abstract":"<div><div>This report presents a systematic review and meta-analysis on the global prevalence of paratuberculosis in sheep, focusing on seropositivity of <em>Mycobacterium avium</em> subspecies <em>paratuberculosis</em> (MAP). This investigation included 1676 records initially, identified from the databases Web of Science, Scopus, Medline and Science Direct. After filtering and reading, twenty-eight articles were selected for the final analysis. The results showed that the pooled prevalence of animals positive for MAP was 8.02 % (95 % CI = 5.04–12.52 %) with significant heterogeneity (I² = 99.3 %), which suggests that there was high variability among results. The prevalence varied according to the continent, such that it was highest in South America (24.45 %) and lowest in Africa (1.34 %). In addition, meta-analysis per herd indicated that the global prevalence was 55.51 % (95 % CI = 44.96–65.58 %), with heterogeneity that was also high (I² = 96.8 %). Analysis of Doi plots demonstrated that there was slight positive asymmetry (LFK index 1.28), which suggests the possibility of publication bias. The meta-analysis demonstrated variation in global prevalence rates of MAP in sheep, with high dissemination among herds, especially in South America and Asia. The heterogeneity of the data indicates methodological differences among studies, thus highlighting the need for standardized investigations to better understand the epidemiology and control of paratuberculosis in sheep globally.</div></div>","PeriodicalId":21758,"journal":{"name":"Small Ruminant Research","volume":"243 ","pages":"Article 107430"},"PeriodicalIF":1.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143161703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel mutations in exon 2 of follistatin (FST) gene associated with wool fiber diameter in sheep","authors":"Tahira Mir,&nbsp;Muhammad Ibrahim,&nbsp;Muhammad Siraj,&nbsp;Sajid Ali Khan Bangash,&nbsp;Sher Hayat Khan,&nbsp;Majid Khan,&nbsp;Muhammad Tayyab,&nbsp;Sohail Ahmad","doi":"10.1016/j.smallrumres.2024.107426","DOIUrl":"10.1016/j.smallrumres.2024.107426","url":null,"abstract":"<div><div><em>Follistatin (FST)</em> is proved to be a key player in hair follicle morphogenesis. This study was designed to investigate the association of SNPs in the <em>FST</em> gene with wool quality traits i.e. fibre diameter, crimp number, and staple length in sheep breeds from Pakistan. Genomic DNA isolated from representative samples of the sheep breeds. A 4444 bp fragment of the <em>FST</em> gene (covering exons 2–6 and the intervening introns) was amplified through PCR. The amplified products were sequenced through Next Generation Sequencing (NGS) technology. The results showed variations in the wool quality parameters among the sheep breeds. Lowest crimp number was recorded for Hashtnagri and Mazai sheep breeds (1.7 ± 0.24 and 1.0 ± 0.12 crimps/cm, respectively). While Kari sheep had the thinnest fibre diameter (27.9 ± 1.19 μm) compared to other sheep breeds. Longest staple length (10.36 ± 0.73 cm) was observed for Ghalji sheep. Sequencing results revealed a total of 51 variants in the targeted region of the <em>FST</em> gene. Kari sheep’s <em>FST</em> gene was the most diverse possessing nine unique variants in the intronic region and two in the coding region. A total of 11 <em>FST</em> variants showed association with fiber diameter, among which two novel missense SNPs (c.229 A&gt;G and c.236 T &gt; C) in the exon 2 were unique to Kari sheep. These SNPs were found to have effect the protein structure and stability and may be used as markers for fine wool having thinner fiber diameter in sheep. These results suggest significant impact of variation in the <em>FST</em> gene on wool fibre diameter.</div></div>","PeriodicalId":21758,"journal":{"name":"Small Ruminant Research","volume":"243 ","pages":"Article 107426"},"PeriodicalIF":1.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143161666","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of the concentration of plasma platelet on the cryopreservation of ram semen","authors":"Luan Sitó-Silva ,&nbsp;Beatriz L. De Camillo ,&nbsp;Luiz Gustavo F. Lima ,&nbsp;Paula Zanin Rattes ,&nbsp;João Carlos Pinheiro Ferreira ,&nbsp;Camila de Paula Freitas-Dell’Aqua ,&nbsp;Paulo F. Marcusso ,&nbsp;Eunice Oba","doi":"10.1016/j.smallrumres.2024.107422","DOIUrl":"10.1016/j.smallrumres.2024.107422","url":null,"abstract":"<div><div>The objective of this study is to assess the effect of autologous platelet-rich plasma (PRP) and platelet-poor plasma (PPP) on the cryopreservation of ovine semen. Eight rams were used, previously trained for collection using an artificial vagina. The collected semen was separated into five parts and diluted in Botubov® (Botupharma, Botucatu, Brazil), and five treatments were tested: Control (pure commercial semen extender); PRP10 (Extender supplemented with 10 million platelets/mL); PRP20 (Extender supplemented with 20 million platelets/mL); PRP40 (Extender supplemented with 40 million platelets/mL); and PPP (Extender supplemented with PPP in the same volume used for PRP40). The semen was then packaged in French straws, cooled for 3 h at 5 °C, and frozen in liquid nitrogen until evaluation. Kinetic parameters were evaluated using computer-assisted sperm analysis (CASA), immediately post-thaw and after a 3-h thermoresistance test at 37 °C, as well as assessment of plasma membrane and acrosomal integrity (PMAI), mitochondrial potential (MP), superoxide anion (O₂) production, and hydrogen peroxide (H₂O₂) production by flow cytometry. Both PRP and PPP were shown to be safe for the cryopreservation of ram semen, with improvements observed in half of the animals in terms of flow cytometry parameters. When grouped together, it was evident that the PPP group displayed greater integrity of the plasma membrane and acrosome (<em>P</em> = 0.02), more stable cells (<em>P</em> = 0.03), and increased production of H₂O₂ (<em>P</em> = 0.05). In conclusion, PRP and PPP are safe and can be viable additives for freezing ram semen. PPP showed better results for plasma and acrosomal membrane integrity, as well as the number of stable cells in half of the animals. This highlights PPP as a promising antioxidant and cryoprotectant for ram semen.</div></div>","PeriodicalId":21758,"journal":{"name":"Small Ruminant Research","volume":"243 ","pages":"Article 107422"},"PeriodicalIF":1.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143161671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Heat stress induced by testicular insulation for 24 or 48 h rapidly impairs epididymal sperm quality and reduces spermatogenesis in rams","authors":"Marina Belucci Teixeira ,&nbsp;João Carlos Pinheiro Ferreira ,&nbsp;Viviane Maria Codognoto ,&nbsp;Eduardo Santos Rossi ,&nbsp;Antonio Guilherme Roncado Pupulim ,&nbsp;Jaqueline Cândido de Carvalho ,&nbsp;Paula Zanin Rattes ,&nbsp;Eunice Oba ,&nbsp;Felipe Martins Negreiro Navolar ,&nbsp;Giovana Wingeter Di Santis ,&nbsp;John Patrick Kastelic ,&nbsp;Ann Van Soom ,&nbsp;Guilherme Rizzoto","doi":"10.1016/j.smallrumres.2025.107443","DOIUrl":"10.1016/j.smallrumres.2025.107443","url":null,"abstract":"<div><div>Mammalian testes must be 3–5 ºC below body core temperature to produce morphologically normal sperm. The objective was to investigate impacts of heat stress (HS) induced by scrotal insulation on epididymal sperm and temporal aspects of HS on spermatogenesis. We hypothesized that: (1) increased testicular temperature impairs sperm in the epididymis; and (2) spermatids are severely impacted by HS exposure. Testicular HS was induced by scrotal insulation for 24 or 48 h in 20 reproductively sound adult rams, with 5 similar rams designated controls (not insulated). Rams were castrated at 24 h, 48 h, 7 d, or 14 d after the start of insulation (whereas control rams were randomly castrated). Insulation increased scrotal surface temperature by ∼5 ºC. There were marked decreases (P &lt; 0.01) in sperm motility, progressive motility and kinetics starting at 24 h and sustained throughout the study. Percentage of epididymal sperm with normal morphology first decreased at 24 h (P &lt; 0.01) with subsequent decreases at 48 h (P &lt; 0.01) and 7 d (P &lt; 0.01); thereafter, morphology remained stable (P &gt; 0.05). At 14 d, there were decreases in testicular weight (P &lt; 0.05) and seminiferous tubule diameter (STD) (P &lt; 0.001) when compared to all other groups. Regarding seminiferous tubule integrity (Johnsen’s score), a first decrease occurred at 24 h (P &lt; 0.05) followed by a more intense decrease at 14 d (P &lt; 0.001). In addition, there was an abrupt decrease (P &lt; 0.05) in spermatid counts at 24 h that was sustained throughout the study. In conclusion, our hypotheses were supported; testicular HS caused immediate deleterious impacts on epididymal sperm at 24 and 48 h post-insulation as well as developing spermatids at 7 and 14 d, decreasing sperm production and significantly reducing both STD and testicular weight.</div></div>","PeriodicalId":21758,"journal":{"name":"Small Ruminant Research","volume":"243 ","pages":"Article 107443"},"PeriodicalIF":1.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143161672","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serodiagnosis of hydatidosis in sheep and goats in Siirt province, Türkiye, and an alternative approach to the interpretation of serology results","authors":"Burcak Aslan Celik ,&nbsp;Muhammed Ahmed Selcuk ,&nbsp;Figen Celik ,&nbsp;Ozgur Yasar Celik ,&nbsp;Muhammet Uslug ,&nbsp;Afra Sena Tekin ,&nbsp;Kerem Ercan ,&nbsp;Sami Simsek","doi":"10.1016/j.smallrumres.2024.107425","DOIUrl":"10.1016/j.smallrumres.2024.107425","url":null,"abstract":"<div><div>Cystic echinococcosis (CE) is one of the most important zoonotic diseases worldwide, affecting both humans and animals, caused by <em>Echinococcus granulosus</em> sensu lato. The diagnosis of CE is based on the evaluation of imaging, serological, molecular and clinical findings. The aim of this study was to develop a diagnostic method that can be used for herd-based diagnosis of CE, which is commonly observed in sheep and goats, and to prevent economic losses by treating positive animals. Antigen B rich partially purified cyst fluid antigen was prepared from a sheep liver hydatid cyst. To determine the sensitivity and specificity of the antigen B-rich partially purified cyst fluid antigen, a Western blot test was first performed using hydatid cyst positive sera from 24 sheep and 20 goats collected from the abattoir and sera from 27 newborn lambs and 23 goat kids as negative controls. Subsequently, 500 sera were collected from 250 sheep and 250 goats from Siirt province and its districts. The strongest band was at 72 kDa, followed by bands at 55 kDa and 28 kDa in SDS-PAGE. According to the 43 kDa band as reference the sensitivity was 62.5 %. and specificity was 96.2 % in sheep. Besides, of the 250 sheep sera collected from the field, and the seroprevalence was 52.80 %. In goats, the sensitivity of WB was 55 % and the specificity was 86.9 %. On the other hand, the seroprevalence was 44.4 % in goats. In conclusion, the production of antigens that are easy to prepare, inexpensive and can be used in field screening for CE will be important in terms of treating positive sheep (even when CE and other helminth parasites are present together) with albendazole and preventing economic losses.</div></div>","PeriodicalId":21758,"journal":{"name":"Small Ruminant Research","volume":"243 ","pages":"Article 107425"},"PeriodicalIF":1.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143161675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Global camel milk industry: A comprehensive overview of production, consumption trends, market evolution, and value chain efficiency","authors":"Omar Ait El Alia ,&nbsp;Yassine Zine-Eddine ,&nbsp;Salah Chaji ,&nbsp;Soumaya Boukrouh ,&nbsp;Khalid Boutoial ,&nbsp;Bernard Faye","doi":"10.1016/j.smallrumres.2025.107441","DOIUrl":"10.1016/j.smallrumres.2025.107441","url":null,"abstract":"<div><div>The global camel population is steadily increasing, with a notable increase in the proportion of camels specifically raised for milk production. This rise is mainly attributed to the surge in demand for camel milk, driven by its distinctive nutritional benefits and recognized health effects. However, studying the global dairy camel sector can provide valuable insights into the development of successful and modernized camel dairy industries, highlighting key strategies for scaling production, improving quality standards, and expanding market reach. This review aims to provide a comprehensive overview of the global evolution of the dairy camel sector. It begins with an analysis of current production levels, consumption patterns, and a detailed examination of camel milk markets and value chain dynamics. Additionally, the camel milk industry faces currently critical barriers related to food hygiene, and limited government support, which hinder quality control and market growth. To address these challenges, a number of potential solutions were proposed such as the implementation of solar-powered cold chains, the necessity for effective training in hygiene and food safety for producers, and the need for investment in infrastructure and climate resilience in order to guarantee the long-term sustainability of the camel milk sector. Hence, this review is supposed to fill the current scarcity of information regarding the status and ongoing developments in the camel milk market, with a particular emphasis on the constraints limiting its growth. By addressing this gap, it seeks to serve as valuable groundwork for producers, industry stakeholders, policymakers, and researchers, thus fostering the development of the camel milk sector.</div></div>","PeriodicalId":21758,"journal":{"name":"Small Ruminant Research","volume":"243 ","pages":"Article 107441"},"PeriodicalIF":1.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143161109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of small ruminant lentiviruses infections on milk yield and milk quality traits in intensively reared dairy sheep","authors":"Aphrodite I. Kalogianni,&nbsp;Athanasios I. Gelasakis","doi":"10.1016/j.smallrumres.2024.107424","DOIUrl":"10.1016/j.smallrumres.2024.107424","url":null,"abstract":"<div><div>Despite small ruminant lentiviruses (SRLV) are widespread causing severe clinical manifestations, their effects on milk production in dairy sheep remain controversial. This study aimed to assess the impact of SRLV infections on milk yield and quality in intensively reared dairy sheep. A total of 527 purebred Chios and Lacaune ewes from four infected intensive dairy sheep farms in Greece were randomly selected and their infection status was determined at pre-lambing using both ELISA (serological testing) and real-time PCR (molecular testing). Their milk production was recorded bimonthly for 4 months for one milking period, initiating at the post-weaning, and physicochemical analyses (fat, protein, lactose, solids-not-fat) and estimation of SCC were performed in individual milk samples. Mixed linear regression models were generated for the assessment of the effects of serological, molecular, and infection status (consideration of both serological and molecular status) on milk production. Prevalence rates of SRLV infections exceeded 60 % in all farms. While serological status had no significant association, molecular status revealed a 10 % reduction in milk yield among infected ewes. Infected seronegative ewes had approximately 20 % less milk, protein, lactose and solids-not-fat yields, and 12 % less fat yield compared to the uninfected ones and approximately 15 % decreased milk, protein, lactose, and solids-not-fat yields, and 7 % decreased fat yield compared to the infected seropositive ones. Our findings underpin the significance of prospective study design and molecular diagnosis to efficiently elucidate the impact of SRLV infections on sheep productivity.</div></div>","PeriodicalId":21758,"journal":{"name":"Small Ruminant Research","volume":"243 ","pages":"Article 107424"},"PeriodicalIF":1.6,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143161704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of antioxidants on quality and DNA methylation of frozen Anglo-Nubian goat semen","authors":"Fareeda Suwor,&nbsp;Pramote Paengkoum,&nbsp;Chatsirin Nakharuthai,&nbsp;Satoshi Kubota,&nbsp;Samorn Ponchunchoovong","doi":"10.1016/j.smallrumres.2025.107447","DOIUrl":"10.1016/j.smallrumres.2025.107447","url":null,"abstract":"<div><div>This study investigated the effects of different concentrations of antioxidants (glutathione, cysteine, and vitamin E) on the cryopreservation of Anglo-Nubian goat sperm. The antioxidants were administered in a tris-based extender designed to achieve iso-osmolality with seminal fluid and contained combinations of soybean lecithin (3 %) and glycerol (5 %). Sperm with motility higher than 70 % was pooled and used for cryopreservation. The sperm was diluted with a tris-based extender and different concentrations of each antioxidant were supplemented at different concentrations (1, 3, and 5 mM for glutathione, 6, 9, and 12 mM for cysteine, and 1, 2, and 3 mM for vitamin E). A sperm sample without antioxidants was also examined, and Andromed was used as a control. Straws were frozen in liquid nitrogen vapor. After storage for 10 days, the frozen straws were thawed at 37 ºC in a water bath for 30 s and evaluated for sperm motility, viability, acrosome integrity, membrane integrity, mitochondrial activity, lipid peroxidation, and DNA methylation. The results showed that the supplementation of 5 mM glutathione in iso-osmolality of the tris-based semen extender improved plasma membrane damage and increased the pregnancy rate compared to the control group (<em>P</em> &lt; 0.05). Furthermore, adding 5 mM glutathione or 6 mM cysteine to the semen extender decreased the lipid peroxidation of the thawed semen (2.76 and 2.80 nmol/mL, respectively). These results were significantly better than those of the other groups (<em>P</em> &lt; 0.05). Nevertheless, an increased concentration of 3 mM of vitamin E in the semen extender increased the MDA concentration (3.78 nmol/mL) in comparison to those obtained with concentrations of 1 mM (2.99 nmol/mL) and 2 mM (3.31 nmol/mL) of vitamin E (<em>P</em> &lt; 0.05). The highest motility rate was 56.10 ± 0.30 % with a viability rate of 58.00 ± 0.45 %, which was achieved with 5 mM glutathione. These results were significantly higher than those of the other treatments (<em>P</em> &lt; 0.05) but lower than those of the control group, which had a motility rate of 68.70 ± 0.470 % and a viability rate of 67.30 ± 0.56 %. In addition, the supplementation of 5 mM glutathione and 6 mM of cysteine did not show a significant difference in sperm DNA methylation compared to fresh sperm (<em>P</em> &gt; 0.05). In summary, this study demonstrated that the addition of 5 mM glutathione to the diluent with iso-osmolality of Anglo-Nubian goat semen has a good effect on frozen semen quality.</div></div>","PeriodicalId":21758,"journal":{"name":"Small Ruminant Research","volume":"244 ","pages":"Article 107447"},"PeriodicalIF":1.6,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143104385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relationship between attributes extracted from carcass image: Prediction of carcass tissue characteristics and meat quality of hair sheep lambs","authors":"Carolina Sarmanho Freitas ,&nbsp;Alinne Andrade Pereira ,&nbsp;Samanta do Nascimento Monteiro ,&nbsp;Gabriel Xavier Serrão ,&nbsp;Jonas Carneiro Araújo ,&nbsp;Manuela Paula de Mesquita Nunes ,&nbsp;Hugo Andrey Santos dos Santos ,&nbsp;Thomaz Cyro Guimarães de Carvalho Rodrigues ,&nbsp;Jamile Andréa Rodrigues da Silva ,&nbsp;Luciara Celi Chaves Daher ,&nbsp;André Guimarães Maciel e Silva ,&nbsp;Welligton Conceição da Silva ,&nbsp;Andréia Santana Bezerra da Silva ,&nbsp;José de Brito Lourenço-Júnior","doi":"10.1016/j.smallrumres.2025.107448","DOIUrl":"10.1016/j.smallrumres.2025.107448","url":null,"abstract":"<div><div>The objective of this work was to propose a methodology for extracting characteristics from shape and texture descriptors of hair lambs carcasses, obtained by digital image processing. Information from 88 cold carcasses of castrated male lambs was divided into three different databases by the views of the images considered and their respective delimitations, with the purpose of evaluating the explanatory quality of these types of captures, as follows: DB1 – “dorsal view database”, aggregating the dorsal view delimitations (dorsal whole carcass + carcass transverse segmentation in dorsal view) with 64 Manifest Variables (MVs) in the IMAGE Latent Variable (LV); DB2 – “sideview database”, with the side view delimitations (sidewhole carcass + carcass transverse segmentation in side view) and 64 MV's in the IMAGE LV; and DB3 – “complete database”, accounting for all dorsal and side delimitations and totaling 128 MV's in IMAGE LV. Regarding the validation of the structural model, referring to the part of the model that evaluates the relationships between the LVs, only the DB1 III and DB3 III models met all the necessary requirements. The PLS-PM was effective in explaining the causal relationships between the variables studied. All models studied obtained sufficient performance to validate the measurement model, with models DB1 and DB2 obtaining better predictive and explanatory performance, with the set of characteristics obtained from the image of the lateral carcass, plus the weight of the cold carcass and the carcass scores, latent variables PRIMAL CUTS and QUALITY.</div></div>","PeriodicalId":21758,"journal":{"name":"Small Ruminant Research","volume":"244 ","pages":"Article 107448"},"PeriodicalIF":1.6,"publicationDate":"2025-01-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143104387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}