Small Ruminant Research最新文献

{"title":"Adding red propolis to the diet of Lacaune lambs: Effects on animal health, ruminal environment, performance, and meat quality","authors":"Guilherme L. Deolindo ,&nbsp;Vitor L. Molosse ,&nbsp;Bruno G.O. Cécere ,&nbsp;Fernanda Picoli ,&nbsp;Luisa Nora ,&nbsp;Charles Marcon ,&nbsp;Bruna Klein ,&nbsp;Bianca F. Bissacotti ,&nbsp;Priscila M. Copetti ,&nbsp;Luiz Eduardo L. Silva ,&nbsp;Roger Wagner ,&nbsp;Aniela P. Kempka ,&nbsp;Denise N. Araujo ,&nbsp;Aleksandro S. da Silva","doi":"10.1016/j.smallrumres.2024.107379","DOIUrl":"10.1016/j.smallrumres.2024.107379","url":null,"abstract":"<div><div>This study determined the influence of red propolis ethanolic extract in lambs' diets on the ruminal environment, animal health, and growth. Two experiments were carried out to achieve these objectives. Pilot study: Twenty-four female Lacaune lambs were divided into four groups, with three replicates and two animals per replicate (pen): T0 (Control), TP1.3, TP2.7, and TP5.3, representing 0, 1.3, 2.7, and 5.3 mL of propolis extract per animal/day, respectively. The highest daily weight gain was observed in lambs that consumed 2.7 mL of propolis extract per day, and they also had the lowest feed conversion rate compared to the control. Lower total leukocyte counts were observed in lambs that consumed propolis extract compared to control due to lower granulocyte and lymphocyte counts. Ceruloplasmin and C-reactive protein levels were lower in the serum of lambs that consumed propolis compared to the control. Lambs that consumed 1.3 and 2.7 mL of propolis extract had lower TBARS levels than the control. The effect of treatment was observed for ruminal pH, which was higher in lambs that consumed propolis extract than in control. A lower concentration of short-chain fatty acids was observed in lambs that consumed propolis than the control due to lower concentrations of acetic and butyric acid. In experiment II, 36 male Lacaune lambs were selected and divided into two groups: MONENSIN (23.45 ppm of monensin per kg/dry matter (DM)) and PROPOLIS (3.8 mL of propolis per animal/day). The animals that consumed propolis extract had greater body weight, weight gain, and average daily gain. These also had a shorter time to reduce methylene blue, thus demonstrating more significant bacterial activity in the rumen. Furthermore, they had lower counts of <em>Escherichia coli</em>, total coliforms, and Enterobacteria. The meat's fatty acid profile in animals fed the red propolis extract showed higher concentrations of monounsaturated fatty acids. In conclusion, adding red propolis extract to the concentrate increased weight gain, average daily gain, and oxidative balance with an antimicrobial and anti-inflammatory effect.</div></div>","PeriodicalId":21758,"journal":{"name":"Small Ruminant Research","volume":"240 ","pages":"Article 107379"},"PeriodicalIF":1.6,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142529736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cryptosporidium and Giardia species infections in sheep from Brazil and associated risk factors","authors":"Lucas Azevedo dos Santos ,&nbsp;Eduardo Henrique Amorim Silva ,&nbsp;Iury Henrique de Freitas Melo ,&nbsp;Ivaldo Victor Mota de Siqueira ,&nbsp;Geiza Marília Paes dos Passos Felix ,&nbsp;Zullay Barros Carvalho de Souza ,&nbsp;Omer Cavalcanti de Almeida ,&nbsp;Tatiene Rossana Móta Silva ,&nbsp;Rafael Antonio Nascimento Ramos ,&nbsp;Gílcia Aparecida de Carvalho","doi":"10.1016/j.smallrumres.2024.107380","DOIUrl":"10.1016/j.smallrumres.2024.107380","url":null,"abstract":"<div><div><em>Cryptosporidium</em> and <em>Giardia</em> genus negatively impact the production of small ruminants. This study aimed to determine the prevalence of infection by <em>Cryptosporidium</em> spp. and <em>Giardia</em> spp. and to assess associated risk factors in sheep from Brazil. We collected fecal samples (n = 459) from animals aged up to twelve months old. Samples were processed using formalin-ether centrifuge-sedimentation techniques with modified Ziehl-Neelsen staining for <em>Cryptosporidium</em> spp. and zinc sulfate centrifuge-flotation for <em>Giardia</em> spp. We calculated the Odds Ratio (OR) to determine associations between different variables and positivity. In total, 27.45 % (n = 126/459; 95 % CI = 23.57 – 31.71) of the animals were infected by <em>Cryptosporidium</em> spp., 13.29 % (n = 61/459; 95 % CI = 10.49 – 16.70) by <em>Giardia</em> spp., and in 3.05 % (n = 14/459; 95 % CI = 1.83 – 5.05) both parasites were detected. <em>Cryptosporidium</em> spp. (χ2 = 28.373; p &lt; 0.0001) predominated in the sheep population. Intensive farming (OR = 3.87; p &lt; 0.0001), herds with 51–80 animals (OR = 3.60; p &lt; 0.0001), no chloride water (OR = 2.0; p = 0.0167), no manure removal (OR = 3.2; p &lt; 0.0001), dirt floors (OR = 6.8; p &lt; 0.0001), and the presence of flies (OR = 2.0; p = 0.0096) were considered risk factors for <em>Cryptosporidium</em> spp. infection. The presence of cohabitating species (OR = 2.1; p = 0.0358) and the presence of dogs or cats (OR = 4.5; p &lt; 0.0001) were risk factors for <em>Giardia</em> spp. infections. Our data demonstrate the need to implement prophylactic actions on the evaluated properties to minimize economic losses and the impact on overall health resulting from infections by <em>Cryptosporidium</em> spp. and <em>Giardia</em> spp.</div></div>","PeriodicalId":21758,"journal":{"name":"Small Ruminant Research","volume":"240 ","pages":"Article 107380"},"PeriodicalIF":1.6,"publicationDate":"2024-10-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142441462","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Physiological responses, ethology, and ingestive behavior of Anglo-Nubiana goats submitted to different air temperatures","authors":"Tiago Gonçalves Pereira Araújo ,&nbsp;Dermeval Araújo Furtado ,&nbsp;Jordânio Inácio Marques ,&nbsp;Patrício Gomes Leite ,&nbsp;Nítalo André Farias Machado ,&nbsp;Neila Lidiany Ribeiro","doi":"10.1016/j.smallrumres.2024.107378","DOIUrl":"10.1016/j.smallrumres.2024.107378","url":null,"abstract":"<div><div>The objective of this study was to evaluate the physiological parameters and ingestive behavior of Anglo-Nubian goats as a function of air temperature, as well as the correlation of these variables. Six male goats were distributed in a completely randomized design in a climate chamber. The treatments consisted of four different controlled AT (20, 24, 28 and 32 °C). The physiological responses: heart rate (HR), respiratory rate (RR) and rectal temperature (RT) were collected at three different times of the day (8 am, 12 pm and 4 pm). The behavioral responses evaluated were: lying inactive, standing inactive, feeding, standing ruminating and lying ruminating. The feed intake decreased linearly in the hours of 4–7 AM, according to the increase in AT. The time spent in the lying ruminating activity decreased linearly (P&lt;0.05) with the increase in AT. The intake and the rate of dry matter intake decreased linearly (P&lt;0.05) with the increase in AT and the water intake increased linearly (P&lt;0.05). The overall results provide information that can be used in heat tolerance studies, helping livestock farmers around the world to plan and make decisions. In addition to contributing to the development of strategies, national and international guidelines for mitigation and accurate assessment of thermal stress.</div></div>","PeriodicalId":21758,"journal":{"name":"Small Ruminant Research","volume":"240 ","pages":"Article 107378"},"PeriodicalIF":1.6,"publicationDate":"2024-10-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142427469","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A review of cashmere fiber phenotypes: Production, heritabilities, and genetic correlations","authors":"Elizabeth A. Dressler ,&nbsp;Jennifer M. Bormann ,&nbsp;Robert L. Weaber ,&nbsp;Roger C. Merkel ,&nbsp;Megan M. Rolf","doi":"10.1016/j.smallrumres.2024.107369","DOIUrl":"10.1016/j.smallrumres.2024.107369","url":null,"abstract":"<div><div>Cashmere is one of the softest, most expensive animal fibers and is produced from the down hair of goats. Cashmere production originated in Asia but now occurs across the world in countries such as Australia, New Zealand, the United Kingdom, and the United States. The Wool Products Labeling Act of 1939, cashmere goat associations, and textile processors have standards for fiber to qualify as cashmere. One of the main qualifying traits for cashmere is mean fiber diameter, usually finer than 19 µm. Additional fiber traits are important to cashmere producers such as down length, down weight, and down yield. Currently, phenotypic selection is one of the only tools available for cashmere producers in many countries to make improvements to these economically relevant fiber traits because most countries do not currently have a national genetic evaluation for cashmere production. Estimation of variance components and genetic correlations from analyses of cashmere fiber traits is limited in literature. Most published genetic analyses were completed in Australia, New Zealand, and Asian countries. Fiber trait definitions and phenotype collection and measurement highly varied between studies, highlighting the need for more standardization. Heritabilities reported in literature for important fiber traits are moderate to high. An antagonistic genetic correlation may exist between mean fiber diameter and other fiber traits such as down yield, down weight, and down length. Because of these potential antagonisms, producers likely need additional tools, such as a properly weighted selection index, to make informed selection decisions. However, heritabilities and genetic correlations reported in literature are dated and often limited to countries outside of the United States. New research initiatives are necessary to upgrade and enhance the current breeding tools available to producers who strive to improve cashmere production, especially in countries which do not currently have a national evaluation for cashmere traits.</div></div>","PeriodicalId":21758,"journal":{"name":"Small Ruminant Research","volume":"240 ","pages":"Article 107369"},"PeriodicalIF":1.6,"publicationDate":"2024-10-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142427976","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assessment of mammary stress in early lactating crossbred (Alpine × Beetal) does during heat stress in conjunction with milk somatic cells and non-invasive indicators","authors":"Lija Satheesan,&nbsp;Aarti Kamboj,&nbsp;Ajay Kumar Dang","doi":"10.1016/j.smallrumres.2024.107375","DOIUrl":"10.1016/j.smallrumres.2024.107375","url":null,"abstract":"<div><div>Milk somatic cell counts (SCC) are crucial for monitoring dairy animals' mammary health and milk quality. This study was initiated to assess the mammary health of early lactating crossbred (Alpine × Beetal) does during periods of heat stress. The udder skin surface temperature (USST), total and differential milk SCC, milk composition, milk cortisol levels and phagocytic activity (PA) of milk neutrophils and macrophages were estimated in these does. Results indicated a significant (p&lt;0.01) increase in the milk neutrophils to macrophages (N: M) ratio and milk cortisol levels with milk somatic cell score (SCS). The PA of milk neutrophils decreased significantly (p&lt;0.01), while that of macrophages increased significantly (p&lt;0.01) with higher SCC. On Pearson correlation, milk SCS revealed strong positive correlation (p&lt;0.01) with N: M ratio (r=0.90), USST (r=0.98), milk cortisol (r=0.97), milk pH (r=0.83) and conductivity (r=0.92). Conversely, milk lactose exhibited a moderate negative correlation of (r= −0.48) with SCS. These findings suggest that USST infrared thermography and milk cortisol levels are promising non-invasive methods for rapidly assessing mammary stress in early lactating does during heat stress.</div></div>","PeriodicalId":21758,"journal":{"name":"Small Ruminant Research","volume":"240 ","pages":"Article 107375"},"PeriodicalIF":1.6,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142427975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of genetic variation in ACADM on slaughter and meat quality traits in Guizhou black goat","authors":"Ziyang Li ,&nbsp;Biao Zhou ,&nbsp;Bo Zhou ,&nbsp;Jiaqi Chen ,&nbsp;Huan Liu ,&nbsp;Xiang Chen ,&nbsp;Yong Ruan","doi":"10.1016/j.smallrumres.2024.107376","DOIUrl":"10.1016/j.smallrumres.2024.107376","url":null,"abstract":"<div><div>The Guizhou black goat is an excellent local goat breed in China, known for its resilience to coarse feed and adaptability to adverse conditions. However, due to hybridization, the production performance of the Guizhou black goat has declined. In this study, we slaughtered 60 Guizhou black goats to assess their slaughter performance and meat quality traits. We analyzed the effects of the <em>ACADM</em> gene on the variation in these traits, particularly focusing on its role in fat deposition and muscle development. Male goats had an overall better slaughter performance than female goats, with significant differences in pre-slaughter live weight, carcass weight, net meat weight, slaughtering percentage, and the thickness of back fat (<em>P</em> &lt; 0.01). As for the meat quality traits, there were extremely significant differences in shear force (<em>P</em> &lt; 0.01), and significant differences in meat color (<em>a</em>* and <em>b</em>* values) and pH value (<em>P</em> &lt; 0.05) between male and female goats. The <em>ACADM</em> g.30914 G&gt;A polymorphism was significantly associated with intramuscular fat content (<em>P</em> &lt; 0.05). The observed association between polymorphisms in the <em>ACADM</em> gene of Guizhou black goats and intramuscular fat content suggests that this gene is a candidate molecular marker for selective breeding.</div></div>","PeriodicalId":21758,"journal":{"name":"Small Ruminant Research","volume":"240 ","pages":"Article 107376"},"PeriodicalIF":1.6,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142427977","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polymorphisms of PRKAA1 and FABP4 genes and their association with feed efficiency in Hu sheep","authors":"Mengru Pu ,&nbsp;Deyin Zhang ,&nbsp;Liming Zhao ,&nbsp;Dan Xu ,&nbsp;Zongwu Ma ,&nbsp;Kunchao Han ,&nbsp;Lijuan He ,&nbsp;Chengqi Yan ,&nbsp;Qi Zhang ,&nbsp;Lianjun Feng ,&nbsp;Ziyue Xiao ,&nbsp;Lei Gao ,&nbsp;Peiliang Cao ,&nbsp;Guoxing Jia ,&nbsp;Dewen Kong ,&nbsp;Linting Li ,&nbsp;Jian Zhang ,&nbsp;Hongjian Li ,&nbsp;Weimin Wang ,&nbsp;Ping Gong ,&nbsp;Huibin Tian","doi":"10.1016/j.smallrumres.2024.107377","DOIUrl":"10.1016/j.smallrumres.2024.107377","url":null,"abstract":"<div><div>Feed efficiency (FE) is an important economic trait in the sheep industry, influenced by biological processes such as digestion and metabolism. <em>PRKAA1</em> gene participates in the synthesis and oxidation of fatty acids; the <em>FABP4</em> gene is closely associated with intracellular fatty acid regulation. In this study, we use Sanger sequencing, KASPar genotyping, and qRT-PCR to investigate the association between variations in <em>PRKAA1</em> and <em>FABP4</em> genes and feed efficiency in Hu sheep, the expression patterns of <em>PRKAA1</em> and <em>FABP4</em> genes in different tissues, as well as the differences in expression of different genotypes in tail fat. The association analysis results indicated that <em>PRKAA1</em> gene g.35431965 C &gt; T locus and <em>FABP4</em> gene g.62829807 C &gt; T locus were significantly associated with the feed conversion ratio (FCR) of Hu sheep aged 80–180 days, with the dominant genotypes being CC and CC, respectively. Simultaneously, there existed notable disparities among various genotype combinations. The qRT-PCR results showed <em>PRKAA1</em> and <em>FABP4</em> genes were widely expressed in ten tissues, of which the <em>PRKAA1</em> gene was lower expression level in rumen and lung, <em>FABP4</em> gene was significantly highest expression in the tail fat than the other tissues; the expression of two different genotypes in tail fat also exhibits significant differences (<em>P</em> ≤ 0.05). Therefore, these findings suggested that the variation of <em>PRKAA1</em> and <em>FABP4</em> genes might be used as novel genetic marker for improving feed efficiency in sheep.</div></div>","PeriodicalId":21758,"journal":{"name":"Small Ruminant Research","volume":"240 ","pages":"Article 107377"},"PeriodicalIF":1.6,"publicationDate":"2024-09-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142427978","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasound assessment of biometric parameters of the head and brain and Doppler indices in cerebral arteries in sheep in the prenatal period","authors":"Barbara Błaszczyk ,&nbsp;Tomasz Stankiewicz ,&nbsp;Małgorzata A. Szewczuk ,&nbsp;Pavitra Chundekkad","doi":"10.1016/j.smallrumres.2024.107374","DOIUrl":"10.1016/j.smallrumres.2024.107374","url":null,"abstract":"<div><div>The aim of this study was to perform a biometric analysis of the head in sheep embryos/fetuses and to determine Doppler parameters in the middle cerebral artery and umbilical artery in 12 prenatal sheep. Using B-Mode ultrasound, the biparietal distance (BPD), occipital-nasal length (ONL), eye length and width, choroid plexus width, and turbinate complex width were determined. Peak systolic velocity (PSV), end diastolic velocity (EDV), PSV/EDV ratio, resistance index (RI) and pulsatility index (PI) were also determined. From the 26th to the 110th day of pregnancy, a progressive increase in BPD and ONL was observed (p&lt;0.01 and p&lt;0.05). The width of the choroid plexus increased until the 96th day of pregnancy (p&lt;0.01 and p&lt;0.05). The PSV in the MCA during the last period of pregnancy was higher than in the first period of pregnancy (p&lt;0.05). A similar trend was noted for EDV (p&lt;0.01). PSV/EDV, RI and PI at the end of pregnancy were the lowest. In the first period of pregnancy, EDV was higher in the MCA than in the UA (p&lt;0.01), but in subsequent periods these differences were reversed (p&lt;0.05, p&lt;0.01). In the first stage of pregnancy, RI and PI in the MCA were lower than in the UA (p&lt;0.01) but in subsequent periods of pregnancy these differences were reversed. The results indicate that several parameters that indicate changes in the heads of prenatal sheep can be detected ultrasonographically.</div></div>","PeriodicalId":21758,"journal":{"name":"Small Ruminant Research","volume":"240 ","pages":"Article 107374"},"PeriodicalIF":1.6,"publicationDate":"2024-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142427468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Quantitative analysis of gametic imprinting effects on productive and reproductive performances of Markhoz goat","authors":"Khabat Kheirabadi","doi":"10.1016/j.smallrumres.2024.107373","DOIUrl":"10.1016/j.smallrumres.2024.107373","url":null,"abstract":"<div><div>In this study, in order to assess the relative importance of gametic imprinting effects for the genetic variation of growth and reproduction traits of Markhoz goat, a data set were obtained from a total of 4983 individuals on productive and reproductive traits of this breed (collected from 1993 to 2017) was analysed. The studied productive traits included body weight (BW) of Markhoz kid at birth and 4 (WW), 6 (W6), 9 (W9), and 12 (W12) months of age, average daily weight gains (g day⁻¹) in the first two growth phases (i.e. from birth to weaning, ADG_0–4; and from weaning to 6-month age, ADG_4–6) and their corresponding Kleiber ratios (i.e. KR_0–4 and KR_4–6). In addition, reproduction traits of interest were the liter size at birth (LSB) and at weaning (LSW), total litter weight at birth (TLWB) and at weaning (TLWW) per does kidding, litter mean weight per kid born (LMWKB) and per kid weaned (LMWKW). Likelihood ratio tests and Akaike’s Information criterion indicated that gametic imprinting effects were significant only for BW, W9 and KR_0–4. Both paternal and maternal gametic imprinting effects had a similar influence on these traits, accounting for between 8 % (W9) and 10 % (BW and KR_0–4) of the total phenotypic variance. For these traits the model with the second-best fit was models including paternal gametic imprinting effects, which indicates crucial paternal gametic imprinting effects in the analyzed traits in the production context. For parentally gametic influenced traits of Markhoz kid, including these effects significantly decreased the direct additive genetic variances (20–94 % depending on the trait) and these reduction were higher when paternal and maternal gametic variance evaluated together than when evaluated separately. Generally these results indicate that when gametic imprinting effects are of importance, but not accounted for, heritability estimates are biased upward and subsequently the realized efficiency of selection is reduced. It was concluded that for more precise estimates of genetic parameters, extracting of overly influential observations from the dataset is necessary.</div></div>","PeriodicalId":21758,"journal":{"name":"Small Ruminant Research","volume":"240 ","pages":"Article 107373"},"PeriodicalIF":1.6,"publicationDate":"2024-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142358852","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The use of PCR method for adulteration detection of goat dairy products manufactured by smallholders","authors":"Anna Dąbrowska","doi":"10.1016/j.smallrumres.2024.107372","DOIUrl":"10.1016/j.smallrumres.2024.107372","url":null,"abstract":"<div><div>The analysis of food adulteration is an important element of improving health safety. Both accidental and intentional adulteration of food, can cause serious health consequences for humans and can be considered as health hazard. In recent years, molecular biology techniques has been increasingly used in food analysis due to their high precision and the ability to obtain results in a short time, as well as the identification and quantitative determination of food adulterants. The samples tested in the study were goat's milk, yogurts, fresh and ripening cheeses produced by smallholders all over Poland. The analysis included DNA isolation, which was used as template for duplex PCR and detection of goat’s and cow’s mitochondrial DNA fragments. The obtained results indicate that the applied technique enabled the rapid identification of adulteration in analyzed goat’s milk products, among which in only one product out of thirty five the presence of undeclared cow’s milk was detected. This indicates good food safety standards applied on small-scale farms.</div></div>","PeriodicalId":21758,"journal":{"name":"Small Ruminant Research","volume":"240 ","pages":"Article 107372"},"PeriodicalIF":1.6,"publicationDate":"2024-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142320086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}