Skin Research and Technology最新文献

{"title":"Thermographic Quantification of Skin Prick Test Responses in Children: The Caloric Allergy Index: Thermographic Analysis: Pediatric Skin Prick Tests.","authors":"Oguzhan Serin, Devrim Onder, Umut Ece Arslan, Umit M Sahiner, Bulent E Sekerel, Ozge Soyer","doi":"10.1111/srt.70363","DOIUrl":"10.1111/srt.70363","url":null,"abstract":"<p><strong>Background: </strong>Skin prick tests(SPTs) are widely used diagnostic tests for evaluating type I hypersensitivity and are interpreted based on human-measured wheal diameters. However, their diagnostic value may be limited by variability in application, measurement, and interpretation due to human dependency. Medical thermography offers a non-invasive, objective, and quantitative method to assess local inflammatory responses. This study aimed to evaluate the feasibility and agreement of thermographic imaging for reading pediatric SPTs and to explore its potential role in supporting a more objective and standardized interpretation.</p><p><strong>Materials and methods: </strong>In this cross-sectional observational study, SPTs were performed on the upper backs of children aged 5-18 using a standardized aeroallergen panel, along with histamine and saline controls. Thermal images were captured at 0, 2, 5, 10, and 15 min using a mobile thermal camera. The Caloric Allergy Index(CAI) was established to quantify allergen-induced skin warming relative to positive and negative controls.</p><p><strong>Results: </strong>Data from 63 pediatric patients were analyzed. Cohen's Kappa scores ranged from 0.432 to 0.838, indicating moderate to almost perfect agreement between CAI values and conventional wheal measurements. Allergens including grass mix(κ = 0.838) and C. dactylis(κ = 0.833) showed the highest agreement, followed by D. pteronyssinus(κ = 0.753) and D. farinae(κ = 0.672). Moderate agreement was observed for tree mix, cat, and dog epithelium. Positive test sites exhibited continuous warming, typically became statistically discernible in 2-5 min, while negative sites showed minimal change.</p><p><strong>Conclusion: </strong>This study introduces a thermographic parameter with overall agreement to conventional SPT readings, supporting the feasibility of thermography as a complementary objective approach in pediatric SPT interpretation.</p>","PeriodicalId":21746,"journal":{"name":"Skin Research and Technology","volume":"32 7","pages":"e70363"},"PeriodicalIF":2.8,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13377611/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148472541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Pediatric Eyelid Lentigo Simplex Treated With a 755-nm Picosecond Alexandrite Laser: A Case Report.","authors":"Xin Tang, Chong Zhang, Qian Ye, Tianyi Zhang, Yan Yan, Baoxi Wang","doi":"10.1111/srt.70370","DOIUrl":"10.1111/srt.70370","url":null,"abstract":"<p><p>Lentigo simplex is a benign melanocytic lesion commonly arising in childhood. We report a 10-year-old girl with a stable periorbital lentigo simplex treated using a 755-nm picosecond alexandrite laser. The patient underwent five treatment sessions over three years, resulting in significant pigment reduction without adverse effects or recurrence. This case highlights the long-term safety and efficacy of picosecond laser treatment for pediatric facial pigmented lesions, particularly in cosmetically sensitive areas. It also adds to the limited literature supporting laser use in children and emphasizes the importance of noninvasive options for aesthetic concerns.</p>","PeriodicalId":21746,"journal":{"name":"Skin Research and Technology","volume":"32 7","pages":"e70370"},"PeriodicalIF":2.8,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13370325/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148449498","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microcore Skin Biopsies: A Novel, Micro-Invasive Skin Biopsy Technique Yielding Full-Thickness Histology.","authors":"Rebecca Lapides, Ga Ram Ahn, Kenechi Iwelumo, Heather Downs, Roger Sumner, Michael McCormack, William G Austen, Elisabeth Roider, Michael Wang-Evers, Dieter Manstein","doi":"10.1111/srt.70362","DOIUrl":"10.1111/srt.70362","url":null,"abstract":"<p><strong>Background: </strong>Punch biopsies are considered the primary technique to obtain full-thickness skin specimens for dermatologic diagnoses. However, limitations include scarring, sampling error, poor margin identification, and post-sampling deformations.</p><p><strong>Objectives: </strong>To introduce a novel micro-biopsy technique utilizing a modified hypodermic needle to achieve full-thickness skin micro-columns and a streamlined post-sampling process for histological analysis.</p><p><strong>Methods: </strong>Ex-vivo skin biopsies were taken using a modified hypodermic needle and compared to 3 mm punch biopsies. They were fixed and processed inside the needle, resulting in a firm, columnar sample, which was then sectioned, stained, and imaged. Dimensions and horizontal shrinkage were also calculated.</p><p><strong>Results: </strong>The technique consistently yielded uniform, columnar-shaped histological samples. These sections were of sufficient quality to perform staining with a significantly smaller horizontal shrinkage rate compared to standard 3 mm punch biopsies.</p><p><strong>Conclusions: </strong>We demonstrate a novel technique for a skin micro-biopsy that allows for fixation and processing of the tissue inside the sampling instrument, yielding small, full-thickness columnar skin histological samples that can be used for dermatological diagnosis with minimal post-sampling deformation.</p><p><strong>Plain language summary: </strong>Punch biopsies are considered the primary technique to obtain full-thickness skin specimens in order to diagnose skin conditions. However, scarring associated with skin biopsies and the deformations that happen to samples during processing pose persistent challenges. To minimize invasiveness, sub-millimeter skin punch biopsies have been introduced, but there has been no standardized, widely adoptable technique to utilize sub-millimeter skin punch biopsies to make diagnoses. Here, we introduce a novel skin biopsy needle that obtains skin samples that are less than 0.5 mm in diameter, which is below the scarring threshold, as well as a processing technique to easily fix, stain, and image the samples to make skin diagnoses.</p>","PeriodicalId":21746,"journal":{"name":"Skin Research and Technology","volume":"32 7","pages":"e70362"},"PeriodicalIF":2.8,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13348449/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148412761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Vivo Microscopic Features of Acne vulgaris Revealed via LC-OCT.","authors":"Daniele Omar Traini, Gerardo Palmisano, Alessandro Di Stefani, Enrico Bocchino, Cristina Guerriero, Mariano Suppa, Ketty Peris","doi":"10.1111/srt.70371","DOIUrl":"10.1111/srt.70371","url":null,"abstract":"<p><strong>Background: </strong>Acne vulgaris is a common skin disorder characterized by multifactorial pathogenesis. In recent years, multiple non-invasive imaging methods have been explored to monitor in vivo acne lesions. Technologies such as reflectance confocal microscopy (RCM) and optical coherence tomography (OCT) have improved our capabilities to characterize and monitor this condition; nevertheless, limitations remain in resolution and depth. Line-field confocal optical coherence tomography (LC-OCT) is a novel imaging technique that combines the strengths of RCM and OCT, offering high-resolution vertical and horizontal imaging of skin tissue up to 500 µm deep. The aim of this study was to characterize and quantify the microstructural features of different acne lesion morphologies using LC-OCT and to compare these findings with those observed in normal skin.</p><p><strong>Methods: </strong>A total of 52 facial acne lesions (microcomedones, closed and open comedones, papules, and pustules) from 20 patients with mild-to-moderate acne and 15 healthy controls were imaged using LC-OCT. Vertical, horizontal, and 3D images were analyzed for structural features, with quantitative measurements of lesion diameter and depth.</p><p><strong>Results: </strong>LC-OCT revealed distinct microstructural features at each stage of acne development. Microcomedones showed bright perifollicular rings in 83.3% of cases, absent in healthy skin, indicating early follicular hyper keratinization. Closed comedones appeared as cystic, keratin-filled structures beneath intact epidermis, while open comedones displayed surface discontinuities and mixed reflectivity plugs. Papules and pustules demonstrated perifollicular hyperreflective halos and numerous \"bright dots\", corresponding to inflammatory cell infiltrates.</p><p><strong>Conclusions: </strong>LC-OCT provides non-invasive, high-resolution imaging of acne microarchitecture, from subclinical changes in acne-prone skin and incipient microcomedones to inflammatory lesions, offering potential utility in diagnosis, disease monitoring and personalized therapeutic strategies.</p>","PeriodicalId":21746,"journal":{"name":"Skin Research and Technology","volume":"32 6","pages":"e70371"},"PeriodicalIF":2.8,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13266275/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148252994","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retinol-Loaded Mannosylerythritol Lipid Nanoliposome for Enhanced Human Skin Penetration.","authors":"Han-Woong Park, Dae-Sung Yoo, Chan Jin Jeong, Soo Nam Park","doi":"10.1111/srt.70357","DOIUrl":"10.1111/srt.70357","url":null,"abstract":"<p><strong>Background: </strong>Functional cosmetic materials with anti-aging properties, such as wrinkle reduction, often struggle to penetrate the skin's stratum corneum (skin barrier). Therefore, to exert anti-aging effects, these materials must pass through the skin barrier and reach fibroblasts in the dermal layer. In this study, deformable liposomes were developed for efficient transdermal delivery of retinol, a well-known anti-wrinkle agent.</p><p><strong>Materials and methods: </strong>Five formulations (ML-1 to ML-5) were prepared using hydrogenated lecithin (HL) and mannosylerythritol lipid (MEL) as an edge activator with HL:MEL mass ratios of 10:0, 7:3, 5:5, 3:7, and 0:10. Their physicochemical properties, including particle size, zeta potential, polydispersity, encapsulation efficiency and deformability index were analyzed. For the optimal formulation, the liposome morphology was observed using cryo-TEM, and the retinol skin penetration ability of each liposome formulation was evaluated using a Franz diffusion cell.</p><p><strong>Results: </strong>Their physicochemical properties were investigated, and ML-4 with an HL:MEL mass ratio of 3:7 was identified as the optimal formulation for transdermal delivery (particle size 89.3±6.8 nm, zeta potential -25.2±1.1 mV, polydispersity index 0.263±0.012, encapsulation efficiency 86.3±2.3%, and deformability index 5.76±1.9). Cryo-TEM analysis confirmed the liposomal morphology of ML-4. The retinol skin penetration ability of each formulation was evaluated using the Franz diffusion cell, with the conventional liposome (ML-1) without MEL as the control. The ML-4 formulation achieved a total retinol penetration of 29.0%, a 10.6% increase compared to ML-1 (18.4%).</p><p><strong>Conclusions: </strong>In this study, the ML-4 deformable liposome formulation, using MEL as an edge activator, efficiently encapsulates retinol, a wrinkle improvement agent, and improves skin penetration without causing physical damage. These findings suggest that ML-4 can serve as an efficient transdermal delivery system for functional cosmetic materials.</p>","PeriodicalId":21746,"journal":{"name":"Skin Research and Technology","volume":"32 6","pages":"e70357"},"PeriodicalIF":2.8,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13238566/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148164440","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Artificial Intelligence in Dermatology: New Regulatory Aspects After the European AI Act.","authors":"Marco Manfredini, Chiara Ciccia Romito, Susanna Viggiani, Caterina Longo","doi":"10.1111/srt.70364","DOIUrl":"10.1111/srt.70364","url":null,"abstract":"","PeriodicalId":21746,"journal":{"name":"Skin Research and Technology","volume":"32 6","pages":"e70364"},"PeriodicalIF":2.8,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13238531/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148164425","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intravital Monitoring of Psoriasis Treatment Response and Drug Delivery Using Multiphoton Fluorescence Lifetime Imaging.","authors":"Lynhda Nguyen, Christian Mess, Katharina Herberger, Stefan W Schneider, Volker Huck","doi":"10.1111/srt.70367","DOIUrl":"10.1111/srt.70367","url":null,"abstract":"<p><strong>Background: </strong>Multiphoton tomography equipped with fluorescence lifetime imaging (MPT-FLIM) is a novel noninvasive imaging technique for analyzing morphological and metabolic states of skin diseases at a subcellular resolution. The present study is the first to establish MPT-FLIM as an imaging modality to monitor treatment response in psoriasis during topical anti-inflammatory therapy.</p><p><strong>Materials and methods: </strong>Patients with psoriasis treated with topical calcipotriol/ betamethasone dipropionate (C/B) or a calcipotriol derivative (C/-) formulation were recruited and monitored using MPT-FLIM. Imaging was performed at baseline on day 0, and during treatment on days 3 and 28.</p><p><strong>Results: </strong>A total of six patients prescribed C/B, six patients prescribed C/- and four healthy controls were recruited. Characteristic histological features were visualized, including acanthosis, parakeratosis, papillomatosis, and thinning of the granular layer. There was a strong correlation between clinical, multiphoton tomographic, and pathophysiological improvement during treatment. Assessment of subclinical metabolic changes was a predictive parameter for treatment outcome. Detection of fluorescence signals from drug components allowed for tracking of drug distribution in intra- and intercellular spaces.</p><p><strong>Conclusion: </strong>MPT-FLIM proved to be a suitable tool for monitoring treatment response in psoriasis patients and tracking drug delivery. It could be a potential method for monitoring other inflammatory skin diseases during treatment to adjust therapy on an individual level.</p>","PeriodicalId":21746,"journal":{"name":"Skin Research and Technology","volume":"32 6","pages":"e70367"},"PeriodicalIF":2.8,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13259963/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148228143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Noninvasive Diagnosis Revealed a Strong Therapeutic Response and Skin Barrier Changes in Hemoporfin-Mediated Photodynamic Therapy for Port-Wine Stains.","authors":"Kai Chen, Jin-Zhao Wu, Yan-Yan Hu, Qian Jiang, Yu-Xin Xia, Jie Chen, Lan Chen, Liu-Qing Chen, Dong-Sheng Li","doi":"10.1111/srt.70369","DOIUrl":"10.1111/srt.70369","url":null,"abstract":"<p><strong>Background: </strong>Noninvasive skin imaging, such as reflectance confocal microscopy (RCM) and optical coherence tomography (OCT), has been extensively used to collect objective clinical data and assess the morphological and ultrastructural change of tissue. However, the application of these noninvasive diagnosis techniques for the therapeutic evaluation of hemoporfin-mediated photodynamic therapy (HMME-PDT) in port-wine stains (PWS) has rarely been reported.</p><p><strong>Objectives: </strong>To explore the clinical efficacy of HMME-PDT for PWS, and assess the application of RCM and OCT in objective assessment of the efficacy of HMME-PDT.</p><p><strong>Methods: </strong>Clinical images, RCM findings, and OCT scans were used to evaluate the treatment efficacy. The vascular diameter and density under RCM findings, the vascular density, and depth of OCT scans were used for objective therapeutic evaluation. Skin surface roughness and barrier functions were used to analyze the skin barrier changes.</p><p><strong>Results: </strong>After PDT treatment, the clinical pictures showed excellent improvements, and the RCM findings revealed significantly decreased vascular diameter (100.0 ±2.450 vs. 59.61± 4.021, p<0.05) and density (16.79 ± 0.6568 vs. 10.91 ± 0.8052) in treated areas. Moreover, the OCT scans further confirmed the decrease in vascular density and depth, and the skin surface roughness of Ra (arithmetic mean), Rz (the average depth of roughness), Rq (the mean square roughness) and the skin barrier function of transepidermal water loss (TEWL), stratum corneum hydration (SCH), and potential of hydrogen (pH) of these treated areas all were significantly changed (p < 0.05).</p><p><strong>Conclusions: </strong>HMME-PDT is an effective and safe treatment option for PWS patients, and noninvasive diagnosis combined with clinical images are excellent techniques for objectively evaluating efficacy of HMME-PDT.</p>","PeriodicalId":21746,"journal":{"name":"Skin Research and Technology","volume":"32 6","pages":"e70369"},"PeriodicalIF":2.8,"publicationDate":"2026-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13269836/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148259560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reduction of Ultraviolet- and Heat-Induced Aging Using Betulin-Loaded Arginine-Caprylate Self-Assembly: Randomized Double-Blind Clinical Trials.","authors":"Koo Chul Kwon, Mi Jung Kim, Sang A Yoon","doi":"10.1111/srt.70361","DOIUrl":"10.1111/srt.70361","url":null,"abstract":"<p><strong>Background: </strong>Betulin exhibits potent antibacterial, anti-inflammatory, and antioxidant properties, effectively preventing ultraviolet (UV)- and heat-induced skin aging. Its limited solubility in cosmetic solvents restricts its application.</p><p><strong>Methods: </strong>To enhance betulin's applicability, a self-assembled nanocarrier, arginine-caprylate ion pair loaded with betulin (B-ACS), was developed. Structural properties, loading efficiency, skin permeability, and antioxidant activity were assessed. UVB-induced damage was examined in human keratinocytes (HaCaT; n = 3) by measuring inflammatory cytokines and matrix metalloproteinases (MMPs) via ELISA. Three-dimensional reconstructed human skin models were exposed to UVA (10 J/cm²) to evaluate collagen and aquaporin-3 protection. A pilot randomized, double-blind, placebo-controlled clinical trial (n = 12, 2 weeks) assessed 3 % (w/w) B-ACS cream on UV- and infrared-induced skin damage.</p><p><strong>Results: </strong>B-ACS showed a particle size of 236.9 ± 10.3 nm, 98.3 ± 1.3 % encapsulation efficacy, and superior reactive oxygen species (ROS) scavenging (IC50 = 4.87 ppm) compared to free betulin (IC50 = 8.26 ppm). In HaCaT cells, B-ACS significantly reduced UVB-induced inflammatory cytokine and MMP expressions (p < 0.05). In reconstructed skin, it prevented UVA-induced collagen and aquaporin-3 degradation. Clinically, 3% B-ACS cream significantly improved elasticity (1.69-fold), brightness (2.59-fold), and pigmentation (1.91-fold); transepidermal water loss (TEWL) and heat-aging markers decreased 1.39-fold and 1.63-fold versus placebo, respectively (p < 0.05).</p><p><strong>Conclusion: </strong>B-ACS enhances betulin's solubility and delivery, offering potent efficacy against UV- and heat-induced aging.</p>","PeriodicalId":21746,"journal":{"name":"Skin Research and Technology","volume":"32 5","pages":"e70361"},"PeriodicalIF":2.8,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13240441/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148006502","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Choosing the Right Tewl Device: A Comparison of Inter- and Intra-Device Reliability of the Vapometer and Dermalab across Measurement Ranges.","authors":"Tanja Klotz, Suzanne Edwards, Guy Maddern, Marcus Wagstaff","doi":"10.1111/srt.70358","DOIUrl":"10.1111/srt.70358","url":null,"abstract":"<p><strong>Background: </strong>Measurement of transepidermal water loss (TEWL) is widely used to evaluate skin barrier function.</p><p><strong>Materials and methods: </strong>This study compared two devices-the DermaLab (DL) TEWL probe and the VapoMeter (VM) - from data collected across three studies to measure TEWL in scarred and normal skin. Each site was measured three times per device, alternating devices to allow vapor clearance. Inter-device reliability was assessed from 1,617 paired locations, each averaged from the three readings. Intra-observer reliability was able to be determined from a dataset of 1,628 DL and 1,635 VM measurements.</p><p><strong>Results: </strong>Data were stratified into low, medium, and high TEWL ranges based on measurement distribution and observations. Overall inter-device reliability was 'good' but varied by range: 'poor' in low, 'excellent' in medium, and 'moderate' in high TEWL ranges. In the medium range, the VM reported nearly twice the TEWL values compared to the DL, while in the high range, it read approximately 34% higher.</p><p><strong>Conclusions: </strong>Both devices showed excellent intra-observer reliability overall. However, in the low TEWL range, the DL exhibited 'moderate' reliability, whereas the VM showed slightly better, 'good' reliability. Differences in chamber architecture-semiopen for the DermaLab and closed for the VM-appear to drive the observed variability in measurement reliability across TEWL ranges. Analysis of this large dataset indicates that the VM likely approaches saturation at the categorized 'high' TEWL values, constraining its accuracy, whereas the DermaLab exhibits reduced measurement stability at low TEWL levels, limiting its applicability under those conditions. Targeted methodological work is needed to refine DL performance in the lowTEWL regime and to more precisely define the upper operational limit of the VM.</p>","PeriodicalId":21746,"journal":{"name":"Skin Research and Technology","volume":"32 5","pages":"e70358"},"PeriodicalIF":2.8,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13140847/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147842424","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}