上海口腔医学最新文献

{"title":"[A clinical control study of bleeding tendency and risk factors in patients with oral antithrombotic drugs after dental extractions].","authors":"Qi Luo, Zhu Zhu, Huan Shi, Qin Zhou, Chuangqi Yu","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Purpose: </strong>To explore the bleeding tendency and related risk factors of patients with or without oral antithrombotic drugs after tooth extraction, in order to provide theoretical reference for perioperative management of tooth extraction.</p><p><strong>Methods: </strong>A cohort study was conducted among patients who attended the Cardiovascular Tooth Extraction Specialist Clinic of Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine from March 2023 to June 2023. The patients were divided into experimental group (medication group) and control group (no medication group) according to whether they took antithrombotic drugs for a long time. Teeth extraction was performed under ECG monitoring and postoperative bleeding was followed up to analyze the incidence of bleeding and related risk factors. SPSS 22.0 software package was used for data analysis.</p><p><strong>Results: </strong>A total of 645 patients were included, including 305 patients in the medication group and 340 patients in the non-medication group. Bleeding events occurred in 17 cases (5.6%, 17/305) in the medication group and 1 (0.3%, 1/340) in the non-medication group, with significant difference between the two groups (P＜0.01). Binary logistic regression analysis showed that old age (OR: 4.089, 95%CI: 1.351-12.374, P=0.013) had a significant positive effect on postoperative bleeding.</p><p><strong>Conclusions: </strong>Oral antithrombotic drugs and advanced age are the risk factors for postoperative bleeding in patients with cardiovascular diseases undergoing tooth extractions.</p>","PeriodicalId":21709,"journal":{"name":"上海口腔医学","volume":"34 2","pages":"146-151"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144476652","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Evaluation of the efficacy of iRoot BP Plus combined with Nd:YAG laser in the treatment of cariogenic pulp exposure].","authors":"Yuying Zhou, Xu Zhang, Juan Yuan","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Purpose: </strong>To evaluate the pulp capping effect of iRoot BP Plus combined with Nd: YAG laser irradiation in the treatment of caries induced exposed pulp and its impact on pulp vitality and occlusal function recovery.</p><p><strong>Methods: </strong>From January 2022 to January 2023, a total of 196 patients with dental caries and exposed pulp, including 268 permanent teeth, were selected and randomly divided into the experimental group of 98 patients (136 teeth) and control group of 98 patients (132 teeth) using computer randomization. Both groups were treated with Nd:YAG laser after cleaning the caries. The experimental group was filled with iRoot BP Plus and the control group was filled with mineral trioxide polymer (MTA). The treatment efficacy, pulp vitality and occlusal function were compared between the two groups. SPSS 24.0 software package was used for statistical analysis of the data.</p><p><strong>Results: </strong>There was no significant difference in the success rate of treatment between the two groups at 3, 6 and 12 months after surgery(P＞0.05). At 12 months after treatment, the bite force and chewing efficiency of the experimental group were significantly higher than those of the control group(P＜0.05). At 3, 6 and 12 months after treatment, the pulp vitality and pulp blood flow in both groups significantly decreased compared to preoperative levels (P＜0.05); the pulp vitality and pulp blood flow of the experimental group were significantly higher than those of the control group(P＜0.05).</p><p><strong>Conclusions: </strong>iRoot BP Plus combined with Nd: YAG laser in the treatment of cariogenic exposed pulp is helpful to preserve pulp vitality and improve occlusal function.</p>","PeriodicalId":21709,"journal":{"name":"上海口腔医学","volume":"34 2","pages":"162-165"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144476604","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[The influence of the relationship between the root apex of the posterior teeth and the maxillary sinus floor on the thickness of the maxillary sinus mucosa].","authors":"Jiongrui Wu, Yiming Gao, Liang Chen","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Purpose: </strong>To measure and analyze the thickness of the maxillary sinus mucosa under different relationships between the maxillary posterior teeth apex and the maxillary sinus floor on cone beam CT (CBCT).</p><p><strong>Methods: </strong>A total of 987 patients requiring implant surgery due to dentition defect or loss were selected from Ruijin Hospital, Shanghai Jiao Tong University School of Medicine from January 2020 to December 2022. The maxillary posterior teeth most closely related to the maxillary sinus were observed on CBCT, the tooth position and root apex were recorded, and the vertical distance between the apex of the posterior tooth and the base of the maxillary sinus was measured. According to the distance between the root apex and the maxillary sinus floor, the posterior teeth were divided into three categories: Class Ⅰ: the distance between the root apex and the maxillary sinus floor (d ＞0 mm); Class Ⅱ : the root apex was attached to the maxillary sinus floor and the cortical bone of the maxillary sinus floor was continuous (d=0 mm); Class Ⅲ : apical protrusion into the maxillary sinus floor(d＜0 mm). The thickness of the thickest part of the maxillary sinus floor mucosa was measured. The distance between the root and the maxillary sinus floor and the thickness of the maxillary sinus mucosa were compared under different root-sinus floor relationship, and the correlation analysis was performed. SPSS 26.0 software package was used to analyze the data.</p><p><strong>Results: </strong>Among the 1974 maxillary sinuses of 987 patients, there were 450 maxillary sinuses with Class I relationship between the posterior root apex and the maxillary sinus floor, while 126 (28%) of the maxillary second molar proximal root and the maxillary sinus floor were the most closely related. In ClassⅠrelationship, the average distance from root to the maxillary sinus floor was (2.9±2.7) mm, and the average thickness of the maxillary sinus mucosa was (2.9±4.0) mm. There was no correlation between the distance from root to the maxillary sinus floor and the thickness of the maxillary sinus mucosa. There were 808 maxillary sinuses with ClassⅡ relationship between the posterior root apex and the maxillary sinus floor, among which 132 (16.3%) maxillary first molars had the closest relationship with the maxillary sinus floor. In Class Ⅱ relationship, the average thickness of the maxillary sinus mucosa was (3.0±4.6) mm. There were 249 maxillary sinuses with Class Ⅲ relationship between the posterior tooth root apex and the maxillary sinus floor, among which 87 (34.9%) palatal roots of the maxillary first molar were the most closely related to the maxillary sinus floor. The average distance from the root to the maxillary sinus floor was (-1.9±-1.4) mm, and the average thickness of maxillary sinus mucosa was (2.4±4.9) mm. There was a positive correlation between them (r=0.18, P=0.004).</p><p><strong>Conclusions: </strong>The most common relationship ","PeriodicalId":21709,"journal":{"name":"上海口腔医学","volume":"34 2","pages":"196-201"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144476623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Application of pedicled buccal fat pad/mucoperiosteal composite flap technique to prevent and seal oroantral fistula].","authors":"Jiaojiao Zou, Xiaoru Hou, Lin Wang, Lifeng Li, Yanqiu Li, Xiaoyi Hu, Xiaotao Xing","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Purpose: </strong>To summarize and evaluate the clinical effectiveness of the pedicled buccal fat pad combined with a mucoperiosteal composite flap in preventing and closing oroantral fistula.</p><p><strong>Methods: </strong>This study included a total of 12 patients who underwent closure of surgical incisions following treatment of posterior maxillary lesions using a pedicled buccal fat pad combined with a mucoperiosteal composite flap. In all cases, the pedicled buccal fat pad was placed beneath the local mucoperiosteum to form a composite flap for incision closure. The retrospective analysis encompassed patients' baseline data, including age, gender, diagnosis, clinical symptoms, imaging characteristics, surgical procedures, and treatment outcomes. Additionally, the analysis included an evaluation of the local functional outcomes in the operative area.</p><p><strong>Results: </strong>Among the 12 patients, there was an equal distribution of males and females (1∶1 ratio), with an average age of (35.83±20.17) years. Among them, 8 cases had cystic lesions in the maxillary posterior region, 3 cases had oroantral fistula, and 1 case had maxillary comminuted fracture. All patients (100%) underwent surgery involving the maxillary posterior dental region adjacent to the maxillary sinus. Preoperative symptoms included swelling, pain, halitosis, and occlusal disorder. CT imaging revealed that bone destruction in the posterior maxilla frequently affected multiple teeth, often with impacted teeth, and showed a close association with the maxillary sinus. Buccal bone resorption or destruction was the predominant feature in the operative area, and after removal of the diseased teeth, limited buccal bone remained. Wound closure was achieved using a buccal fat pad/mucoperiosteal composite flap technique. During the follow-up period, all 12 patients healed successfully, with no occurrences of oral-nasal fistula. Additionally, no significant changes in buccal morphology or vestibular groove were observed.</p><p><strong>Conclusions: </strong>The use of a pedicled buccal fat pad combined with a mucoperiosteal composite flap is effective in sealing and preventing oroantral fistula, improving clinical symptoms in cases involving oroantral fistula and maxillary posterior bone resorption during surgery.</p>","PeriodicalId":21709,"journal":{"name":"上海口腔医学","volume":"34 2","pages":"218-224"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144476580","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Comparison of biomechanical effects and tissue reparability of photodynamic and laser in the adjuvant treatment of peri-implantitis].","authors":"Xiao Zhang, Hui Yang","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Purpose: </strong>To compare the biomechanical effects and tissue reparability of photodynamic and laser in the adjuvant treatment of peri-implantitis.</p><p><strong>Methods: </strong>A total of 112 patients with peri-implantitis who were diagnosed and treated from March 2023 to April 2024 were randomly divided into the control group and the experimental group, with 56 cases in each group. On the basis of conventional symptomatic treatment, the control group received photodynamic therapy and the experimental group received laser therapy. The biomechanical effects [implant mobility(IM), peri-implant bone density(PBD), mesial and distal marginal bone resorption of the implant], tissue reparability[sulcus bleeding index (SBI), plaque index (PLI) and probing depth (PD)], inflammatory factors [interleukin-6 (IL-6), tumor necrosis factor (TNF-α), and matrix metalloproteinases-8(MMP-8)] and the proportion of Porphyromonas gingivalis(P.g) in the total bacterial count were compared before and 1, 2, and 3 months after treatment between the two groups. SPSS 26.0 software package was used for statistical analysis of the data.</p><p><strong>Results: </strong>After 1-3 months of treatment, both groups showed significant decrease in IM, mesial and distal marginal bone resorption of the implant, SBI, PLI, PD, IL-6, TNF-α, MMP8, and the proportion of P.g to total bacteria (P＜0.05), and significant increase in PBD (P＜0.05). Compared with the control group, the experimental group showed significant improvements in IM, PBD, mesial and distal marginal bone resorption of the implant, IL-6, TNF-α and MMP8 (P＜0.05). Compared with the experimental group, the control group showed significant improvements in SBI, PLI, PD and the proportion of P.g to total bacteria (P＜0.05).</p><p><strong>Conclusions: </strong>Both photodynamic and laser therapy can be used in the adjuvant treatment of peri-implantitis. Photodynamic therapy has advantages in tissue repair and reducing the proportion of P.g to total bacteria, while laser therapy has advantages in biomechanical effects and reducing inflammatory factors.</p>","PeriodicalId":21709,"journal":{"name":"上海口腔医学","volume":"34 2","pages":"213-217"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144476582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Study on the correlation between the expression of Porphyromonas gingivalis, tumor-associated macrophages and the clinical prognosis of oral squamous cell carcinoma].","authors":"Muqiu Li, Chenxi Li, Wei Wei, Cheng Chen, Bing Wang, Zhongcheng Gong, Wei Guo, Guoxin Ren","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Purpose: </strong>To explore the immunoexpression and clinical significance regarding Porphyromonas gingivalis (P.gingivalis) and tumor-associated macrophage (TAM) in the tumor microenvironment of oral squamous cell carcinoma (OSCC).</p><p><strong>Methods: </strong>A total of 200 OSCC patients admitted to the First Affiliated Hospital of Xinjiang Medical University between June 2008 and June 2020 were divided into P.gingivalis-strong-expression group(n=139) and P. gingivalis-weak-expression group (n=61), based on the immunohistochemical(IHC) staining. The detection of DOK3 and TAM was performed to quantify their expression level in two groups, respectively. The associations between the three mentioned biomarkers and clinicopathologic indicators were compared between the two groups, and the impact on patients' prognosis was also analyzed. R 4.2.2 software was used for data analysis.</p><p><strong>Results: </strong>Death, late-stage cancer, T3-T4 and N(+) stage, large size (≥3 cm), moderate and poor differentiation, and recurrence exhibited significant associations with strong immunoexpression of P. gingivalis(P＜0.05). Several variables of OSCC patients with strong immunoexpression of P. gingivalis including age, survival status, T and N stage, recurrence exhibited significant associations with strong immunoexpression of DOK3 (P＜0.05); whereas T stage, clinical stage and tumor differentiation exhibited significant associations with strong immunoexpression of TAM (P＜0.05). Additionally, the immunoexpression of P. gingivalis was positively associated with the immunoexpression of DOK3 and TAM(P＜0.01). The prognosis of OSCC patients with strong positive expression of P. gingivalis, DOK3 and TAM was worse than that of patients with weak immunoexpression, respectively(P＜0.05).</p><p><strong>Conclusions: </strong>P. gingivalis is positively correlated with DOK3 and TAM. In addition, the strong immunoexpression levels of P. gingivalis, DOK3 and tumor-associated macrophage are associated with poor prognosis in patients with OSCC. Collectively, the mentioned three biomarkers can be considered as important indicators for evaluating clinical prognosis.</p>","PeriodicalId":21709,"journal":{"name":"上海口腔医学","volume":"34 2","pages":"132-138"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144476610","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[The study on the effects of different shapes of nasopalatine canals on tooth movement and implantation during orthodontic and implant treatment with CBCT].","authors":"Wanyu Zhu, Xiaoyu Shi, Jie Yang, Jing Nie","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Purpose: </strong>The aim of this study was to evaluate the impact of the nasopalatine canal(NPC) shape and its morphometric characteristics on expected teeth movement by assessing the distance to maxillary central incisors (MCIs) according to NPC type.</p><p><strong>Methods: </strong>A total of 200 patients with CBCT imaging data meeting the inclusion criteria were selected. The nasopharyngeal canal in the sagittal plane was classified. The following parameters were obtained: incisor foramen diameter, nasopalatine canal length, anterio-posterior diameter of incisor foramen (A-P IF), anterio-posterior diameter of Stenson foramen (A-P NF), nasopalatine tube 1/2 length diameter(M-L IF), and the distance between NPC and MCIs. The data were analyzed with SPSS 26.0 software package.</p><p><strong>Results: </strong>Diameter of incisor hole（P=0.045）, A-P IF（P=0.001）, M-L IF（P＜0.001）, A-P NF（P＜0.001） had significant difference. In cylindrical-shaped NPC, a significant correlation was observed for NPC length, while in funnel-shaped NPC, a significant correlation was observed for A-P IF and NPC length. In hourglass-shaped NPC, a significant correlation was observed for A-P IF, A-P NF, and NPC length.</p><p><strong>Conclusions: </strong>Each NPC shape showed specific impacts on the relationship between NPC diameters at different sections and distances to MCIs.</p>","PeriodicalId":21709,"journal":{"name":"上海口腔医学","volume":"34 2","pages":"171-176"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144476624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Measurement of deciduous canine and construction of standardized virtual crown based on ray projection].","authors":"Xiaoxue Sang, Zhimin Ding, Fei Yan, Ning Yao, Jiaxin Kong, Liyun Zhai, Youmin Mei, Dafeng Ji","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Purpose: </strong>To explore the construction process of a standard virtual crown for deciduous canine teeth, provide a foundation for the establishment of a standard crown model for deciduous canine teeth, and reference and basis for the application of CAM/CAM technology in pediatric dentistry.</p><p><strong>Methods: </strong>A total of 65 children aged 4-5 years old with caries free deciduous canine were selected from the Imaging Department of Nantong Stomatological Hospital, including 34 boys and 31 girls. The obtained DICOM format data was imported into 3DSlicer 5.0.3 software for ray projection rendering and reconstruction. Using the ruler tool in the software, crown data of deciduous teeth were extracted, crown width, thickness and length were measured, and the data were divided into male and female, upper and lower deciduous teeth for Student's t test. The reconstructed model was modified according to the test results, and the mean and median models of crown width, thickness and length of male/female upper/lower deciduous teeth were established. A vernier caliper was used to measure the isolated milk fangs. The results of virtual measurement and specimen measurement were compared by Student's t test. MATLAB R 2016a software was used for statistical analysis.</p><p><strong>Results: </strong>There were significant differences in the three radial directions between males and females; There was a statistical difference in crown width and thickness between the upper and lower deciduous canines, but there was no significant difference in crown length. The average values of crown width, crown thickness, and crown length of girls' maxillary deciduous canines were 6.20, 3.97, and 5.61 mm, respectively, with a median of 6.15, 3.82 and 5.53 mm. The average values of crown width, crown thickness, and crown length of girls' mandibular deciduous canines were 5.47, 4.25 and 5.66 mm, respectively, with median values of 5.45, 4.22 and 5.68 mm, respectively. The average values of crown width, crown thickness, and crown length of male primary teeth were 6.40, 4.14 and 5.87 mm, respectively, with median values of 6.40, 4.04 and 6.05 mm. The average values of crown width, crown thickness, and crown length of male mandibular deciduous canines were 5.69, 4.53 and 6.00 mm, respectively, with median values of 5.64, 4.65 and 6.06 mm. Based on the above values, virtual crown mean and median models were established for male/female maxillary and mandibular deciduous canines.</p><p><strong>Conclusions: </strong>The diameter values obtained by ray projection and measurement in 3DSlicer have higher reliability compared to specimen measurements. The measurement results can be used to establish a virtual model of the male/female upper and lower primary canine standard crown, providing a reference for the establishment of the primary canine standard crown model.</p>","PeriodicalId":21709,"journal":{"name":"上海口腔医学","volume":"34 2","pages":"177-183"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144476607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Clinical application of prosthetic wall made by orthodontic band in vital pulp therapy of young permanent teeth].","authors":"Yu-Ting Guo, Hong Li, Hong-Bin Wu, Qi Teng, Yan-Yan Liu, Sun Wang, Chang-Yu Cai","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>PURPOSE： To investigate the clinical application value of making false wall with orthodontic band when a large area of tissue defects of young permanent teeth is subgingival level and vital pulp therapy is required.</p><p><strong>Methods: </strong>A total of 60 cases of young permanent molars with large tissue defects to subgingival level and requiring vital pulp therapy were selected and randomly divided into experimental group and control group, with 30 cases in each group. Orthodontic band was made in the experimental group, while traditional composite resin was used in the control group, rubber barrier was placed after the fabrication of the false wall. The clinical fabrication time, stability of the false wall after the use of rubber barrier and periodontal health conditions(SBI, GI, SPD) of the affected tooth before and after operation were compared between the two groups.Graph Pad Prism 5.0 software package was used for statistical analysis.</p><p><strong>Results: </strong>The fabrication time of the experimental group was (6.01±0.34) min, which was shorter than that of the control group (12.02±0.53) min, and the difference was statistically significant(P＜0.05). But the stability of the false wall and the influence on the periodontal health of the affected teeth were not significantly different between the two groups(P＞0.05).</p><p><strong>Conclusions: </strong>For the cases of young permanent teeth with large area defects to subgingival and requiring vital pulp therapy, orthodontic band can shorten the fabrication time of false wall, provide relatively stable retention, with no significant effect on the periodontal status of the affected teeth in a short time. It can provide a new idea for the fabrication of false wall in pediatric dental clinical work.</p>","PeriodicalId":21709,"journal":{"name":"上海口腔医学","volume":"34 1","pages":"79-82"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144012640","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Construction and application of organoid radiological model of patient-derived salivary gland malignant tumors].","authors":"Feng Xu, Jian Meng, Lin Chen, Yin-Yu Chen, Shou-Peng Wang","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Purpose: </strong>To construct a brachytherapentic model of patient-derived salivary gland malignant tumor organoids, and evaluate the impact of 125I radioactive seeds intervention on the activity of different organoids, and explore the feasibility of using this model to guide clinical personalized treatment of salivary gland malignant tumors.</p><p><strong>Methods: </strong>Biopsy specimens from patients with malignant salivary gland tumors including mucoepidermoid carcinoma (MEC), adenoid cystic carcinoma(ACC) and acinic cell carcinoma (AciCC) from Xuzhou Central Hospital were collected, and 3D culture method was used to construct the organoid model. The expression levels of immunohistochemical indicators in salivary gland malignant tumor tissue samples and organoid samples were detected. Brachytherapentic model was constructed and the effect of different doses of 125I seeds on organoid activity were detected using 3D cell viability assay. The activity curves were plotted and the IC50 values were calculated. The data were analyzed by GraphPad Prism 9.0 software.</p><p><strong>Results: </strong>H-E staining results and immunohistochemical staining results identified homology between organoids and specimen tumors. The mean IC50 values of 125I seeds on 5 cases of salivary gland malignant tumor organoids (1 case of ACC, 2 cases of AciCC and 2 cases of MEC) were 8 508, 6 053, 5 203, 10 379 and 7 743 cGy, respectively. AciCC has a higher sensitivity to 125I seeds, and the sensitivity of 125I seeds varied among individuals of the same type of tumor.</p><p><strong>Conclusions: </strong>An in vitro tumor organoid culture platform has been established for specimens from patients with salivary gland malignant tumors, and its morphological, histological structure, and molecular marker expression are consistent with the characteristics of tumor cells in the original specimens. Intervention results indicated that the model has significant individualized differences, providing a theoretical basis and methodological reference for the effectiveness of 125I seeds in some patients with local, recurrent, advanced, poorly differentiated, or unresectable tumours. This model has the advantages of low cost, short modeling time, and high throughput detection. It can predict the patient's post-treatment response in vitro, guide risk assessment of malignant tumors in clinical practice, and establish personalized and precise treatment plans.</p>","PeriodicalId":21709,"journal":{"name":"上海口腔医学","volume":"34 1","pages":"25-31"},"PeriodicalIF":0.0,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144016957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}