[Study on the correlation between the expression of Porphyromonas gingivalis, tumor-associated macrophages and the clinical prognosis of oral squamous cell carcinoma].

Q4 Medicine
上海口腔医学 Pub Date : 2025-04-01
Muqiu Li, Chenxi Li, Wei Wei, Cheng Chen, Bing Wang, Zhongcheng Gong, Wei Guo, Guoxin Ren
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引用次数: 0

Abstract

Purpose: To explore the immunoexpression and clinical significance regarding Porphyromonas gingivalis (P.gingivalis) and tumor-associated macrophage (TAM) in the tumor microenvironment of oral squamous cell carcinoma (OSCC).

Methods: A total of 200 OSCC patients admitted to the First Affiliated Hospital of Xinjiang Medical University between June 2008 and June 2020 were divided into P.gingivalis-strong-expression group(n=139) and P. gingivalis-weak-expression group (n=61), based on the immunohistochemical(IHC) staining. The detection of DOK3 and TAM was performed to quantify their expression level in two groups, respectively. The associations between the three mentioned biomarkers and clinicopathologic indicators were compared between the two groups, and the impact on patients' prognosis was also analyzed. R 4.2.2 software was used for data analysis.

Results: Death, late-stage cancer, T3-T4 and N(+) stage, large size (≥3 cm), moderate and poor differentiation, and recurrence exhibited significant associations with strong immunoexpression of P. gingivalis(P<0.05). Several variables of OSCC patients with strong immunoexpression of P. gingivalis including age, survival status, T and N stage, recurrence exhibited significant associations with strong immunoexpression of DOK3 (P<0.05); whereas T stage, clinical stage and tumor differentiation exhibited significant associations with strong immunoexpression of TAM (P<0.05). Additionally, the immunoexpression of P. gingivalis was positively associated with the immunoexpression of DOK3 and TAM(P<0.01). The prognosis of OSCC patients with strong positive expression of P. gingivalis, DOK3 and TAM was worse than that of patients with weak immunoexpression, respectively(P<0.05).

Conclusions: P. gingivalis is positively correlated with DOK3 and TAM. In addition, the strong immunoexpression levels of P. gingivalis, DOK3 and tumor-associated macrophage are associated with poor prognosis in patients with OSCC. Collectively, the mentioned three biomarkers can be considered as important indicators for evaluating clinical prognosis.

[牙龈卟啉单胞菌、肿瘤相关巨噬细胞表达与口腔鳞状细胞癌临床预后的相关性研究]。
目的:探讨口腔鳞状细胞癌(OSCC)肿瘤微环境中牙龈卟啉单胞菌(P.gingivalis)和肿瘤相关巨噬细胞(TAM)的免疫表达及其临床意义。方法:选取2008年6月~ 2020年6月新疆医科大学第一附属医院收治的200例OSCC患者,根据免疫组化(IHC)染色分为龈假单胞菌强表达组(139例)和龈假单胞菌弱表达组(61例)。分别检测两组细胞中DOK3和TAM的表达量。比较两组间上述三种生物标志物与临床病理指标的相关性,并分析其对患者预后的影响。采用r4.2.2软件进行数据分析。结果:死亡、晚期癌、T3-T4和N(+)期、大体积(≥3cm)、中低分化、复发与牙龈假单胞菌免疫表达强相关(P<0.05)。龈假单胞菌免疫表达强的OSCC患者年龄、生存状态、T、N分期、复发与DOK3免疫表达强相关(P<0.05);而T分期、临床分期和肿瘤分化与TAM的高表达有显著相关性(P<0.05)。此外,牙龈假单胞菌的免疫表达与DOK3和TAM的免疫表达呈正相关(P<0.01)。龈假单胞菌、DOK3和TAM免疫表达强阳性的OSCC患者预后差于免疫表达弱的患者(P<0.05)。结论:牙龈卟啉卟啉菌与DOK3、TAM呈正相关。此外,牙龈假单胞菌、DOK3和肿瘤相关巨噬细胞的高免疫表达水平与OSCC患者预后不良有关。综上所述,上述三种生物标志物可作为评价临床预后的重要指标。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
上海口腔医学
上海口腔医学 Medicine-Medicine (all)
CiteScore
0.30
自引率
0.00%
发文量
5299
期刊介绍: "Shanghai Journal of Stomatology (SJS)" is a comprehensive academic journal of stomatology directed by Shanghai Jiao Tong University and sponsored by the Ninth People's Hospital Affiliated to Shanghai Jiao Tong University School of Medicine. The main columns include basic research, clinical research, column articles, clinical summaries, reviews, academic lectures, etc., which are suitable for reference by clinicians, scientific researchers and teaching personnel at all levels engaged in oral medicine.
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