上海口腔医学最新文献

{"title":"[The impact of oral occlusal exercises on occlusal performance of oral cancer patients following segmental mandibulectomy without simultaneous reconstruction].","authors":"Ling Yang, Tian Zhou, Ning Zhao, Xingzhou Qu, Weihong Chen, Yan Zhao, Lili Hou","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Purpose: </strong>To investigate the effects of oral occlusion exercises on occlusal performance of oral cancer patients who underwent segmental mandibular resection without simultaneous reconstruction.</p><p><strong>Methods: </strong>From October 2022 to May 2023, a total of 63 oral cancer patients who underwent segmental mandibular resection without reconstruction at the Department of Oromaxillofacial Head and Neck Oncology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine were selected. They were randomly divided into control group (30 patients) and experimental group (33 patients). The control group received conventional care, while the experimental group underwent oral occlusion exercises except conventional care. After three months, the maximum total occlusal force(TOF), asymmetry index of occlusal force(AOF), center of occlusal force(COF), and mastication efficiency of both groups were compared. Statistical analysis was conducted using SPSS 26.0 software package.</p><p><strong>Results: </strong>Compared with the control group, TOF in the experimental group was increased, AOF and COF were decreased, and the differences were statistically significant(P＜0.01). The masticatory efficiency of the experimental group was significantly better than that of the control group(P＜0.01).</p><p><strong>Conclusions: </strong>Oral occlusal exercises can improve occlusal performance in patients with mandibular resection without reconstruction, but larger sample size and rigorous design are needed to further verify its clinical effect and mechanism.</p>","PeriodicalId":21709,"journal":{"name":"上海口腔医学","volume":"34 2","pages":"184-190"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144476611","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[A study on the effectiveness of different types of decalcifying solutions in the preparation of combined oral and maxillofacial tooth-mandibular-periodontal tissues].","authors":"Ting Gu, Min Wang, Yu Wang, Jiang Li, Chunye Zhang","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Purpose: </strong>Comparative analysis of MORSE, formic acid and Plank-Rychlo decalcification solution for the treatment of combined tooth-mandibular-periodontal tissues to explore their application value.</p><p><strong>Methods: </strong>Specimens including normal teeth, jaws and periodontal tissues discarded from Department of Oral Pathology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine were collected and randomly divided into 3 groups to analyse the differences in decalcification time, staining effect and nucleic acid integrity of 3 different decalcification solutions. SPSS 23.0 software package was used for statistical analysis.</p><p><strong>Results: </strong>The mean decalcification time was 4.5, 3.5 and 3.6 days in the MORSE, formic acid and Plank-Rychlo group, respectively. H-E staining scores were in descending order in the MORSE, formic acid and Plank-Rychlo group. Immunohistochemical staining showed that AE1/AE3, p63 and Ki67 scores in MORSE group were better than those in the formic acid group and the Plank-Rychlo group, and Vimentin staining indices showed no significant difference between the 3 groups. In the fluorescence in situ hybridisation results, normal fluorescence signals were detected in 15 cases in MORSE group, 1 case in formic acid group and 4 cases in Plank-Rychlo group. The mean DNA concentration was 1.987, 1.963 and 1.115 ng/μL in MORSE, formic acid and Plank-Rychlo group, respectively; and the mean RNA concentration was 13.03, 11.08 and 1.66 ng/μL, respectively. There was no significant difference in DNA concentration and RNA concentration between MORSE and formic acid group(P＞0.05), and both DNA and RNA concentrations were higher in the 2 groups than those in Plank-Rychlo group(P＜0.001).</p><p><strong>Conclusions: </strong>MORSE decalcification solution has comprehensive advantages in the treatment of combined tooth-mandibular-periodontal tissues and is of value in the clinical, teaching and research aspects of pathology.</p>","PeriodicalId":21709,"journal":{"name":"上海口腔医学","volume":"34 2","pages":"113-118"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144476578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[A study on the relationship between etiology and influencing factors of burning mouth syndrome].","authors":"Huirong Zhou, Xiaoping Lin","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Purpose: </strong>To analyze the etiology and related influencing factors of burning mouth syndrome (BMS).</p><p><strong>Methods: </strong>A total of 109 patients with BMS who visited the Department of Stomatology of Shengjing Hospital of China Medical University from November 2022 to October 2023 were selected, and 84 healthy volunteers who participated in the survey during the same period were selected as the control group. The basic information of the research subjects was recorded, and oral health status, periodontal health status and psychological status of the research subjects were evaluated. Student's t test, Chi-square test and binary logistic regression analysis were used to analyze the factors affecting the incidence of BMS with SPSS 27.0 software package.</p><p><strong>Results: </strong>Mono-factor analysis showed that residual crown, residual root, periodontitis, anxiety status, depressive status, sleep disorders, menopause, diabetes, digestive system diseases and lacunar infarction were related to the incidence of BMS (P＜0.05). The variables with P＜0.1 in mono-factor analysis were selected for multivariate logistic regression analysis. The results showed that periodontitis, COVID-19, anxiety status, depressive status, sleep disorders, lacunar infarction, and digestive system diseases were factors affecting the incidence of BMS (P＜0.05 and OR＞1).</p><p><strong>Conclusions: </strong>The diagnosis and treatment of BMS require multidisciplinary cooperation of specialists such as psychiatry, neurology and gastroenterology, and active treatment of systemic or related diseases. In addition to strengthening oral hygiene and periodontal health management for patients, clinical practitioners should also pay attention to the aggravation of BMS patients' pain intensity, sleep quality and anxiety caused by COVID-19 pandemic, and provide timely psychological counseling to better control the development of BMS.</p>","PeriodicalId":21709,"journal":{"name":"上海口腔医学","volume":"34 2","pages":"139-145"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144476579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Retrospective analysis of 956 cases with dental trauma].","authors":"Jinheng Li, Junjun Zhao, Tiantian Wu, Zhaowei Tai, Yiyun Dong","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Purpose: </strong>To find the clinical characteristics of permanent teeth trauma among young and adult people in Shanghai Ninth People's Hospital, and provide reference for the treatment and prevention of dental trauma among them.</p><p><strong>Methods: </strong>The data of 956 patients who visited the Dental Trauma Clinic of Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine from September 2021 to March 2023 were collected and analyzed according to age, gender, occupation, causes of trauma, types of tooth injuries, number and position of affected teeth, time to seek treatment after injury, and whether the patients had been exposed to popular science knowledge related to tooth trauma.</p><p><strong>Results: </strong>The male to female ratio of dental trauma patients was 0.93∶1. The tooth position with the highest incidence of dental trauma was the maxillary central incisor, followed by the maxillary lateral incisor. Subluxation and uncomplicated crown fractures were the subcategories with the highest incidence of dental trauma. For the post-traumatic treatment time, 55.54% of patients sought medical consultation within 1-4 hours. Survey of the occupation of patients with dental trauma found that high-risk exposure accounted for only 7%. Among them, the highest risk occupation were builders and men for takeaway. Falls and traffic accidents were the main causes of dental trauma. Only 8% of the patients received scientific knowledge related to dental trauma.</p><p><strong>Conclusions: </strong>Tooth injuries are more common in the upper incisors, and the types of injuries are more common in crown fractures and subluxations. The main causes are falls and traffic accidents. Dentists should strengthen the popularization of knowledge about dental trauma, and the public should pay attention to strengthening the rational use of mobile phones, raising road safety awareness, and learning emergency treatment methods for dental trauma.</p>","PeriodicalId":21709,"journal":{"name":"上海口腔医学","volume":"34 2","pages":"191-195"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144476608","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Application of sliding vermilion flap in angulus oris defect after buccal mucosa cancer ablation].","authors":"Kun Wu, Hanjiang Wu","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Purpose: </strong>To evaluate the effect of sliding vermilion flap in angulus oris defect after buccal mucosal cancer ablation.</p><p><strong>Methods: </strong>A total of 33 patients with buccal mucosal cancer who underwent buccal mucosal cancer radical surgery and resection of angulus oris, and reconstructed with free anterolateral thigh flap transfer and sliding vermilion flap were collected from January 2015 to April 2021 in Department of Oral and Maxillofacial Surgery at Second Xiangya Hospital. Thirty-three patients with buccal mucosal cancer admitted to Department of Oral and Maxillofacial Surgery, Second Xiangya Hospital of Central South University from January 2015 to April 2021 were selected. All patients underwent combined buccal-mandibulectomy and neck radical operation and resection of oral angle, and underwent anterolateral femoral free flap and lip red elastic flap reconstruction. The angulus oris outcome, aesthetics outcome, masticatory efficacy, swallowing and speech function were assessed by FACT-HN (The Functional Assessment of Cancer Therapy-Head and Neck). The mouth opening was measured and recorded. SPSS 19.0 software package was used for statistical analysis of the data.</p><p><strong>Results: </strong>The success rate of free flap transplantation was 100% in 33 patients. Although the aesthetics outcome was decreased at postoperative 1 year compared with that of preoperative duration (P＜0.001), there was no significant difference of angulus oris outcome at postoperative 1 year compared with that of preoperative duration (P＞0.05). Moreover, the masticatory function was significantly improved at postoperative 1 year compared with that of preoperative duration(P=0.004). However, there was no significant difference of speech and swallowing function at postoperative 1 year compared with that of preoperative duration (P＞0.05). The mouth opening was significantly improved at postoperative 1 year compared with that of preoperative duration(P＜0.05), and the satisfaction of mouth opening in these patients was 75.7%.</p><p><strong>Conclusions: </strong>The use of sliding vermilion flap can ideally repair the angulus oris, and offer a novel method to reconstruct defect of angulus oris.</p>","PeriodicalId":21709,"journal":{"name":"上海口腔医学","volume":"34 2","pages":"157-161"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144476581","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Comparison of the effect of glass fiber and cobalt alloy post-core all-ceramic crowns on the restoration of anterior teeth].","authors":"Kaili Dong, Shanshan Lyu, Min Zhang","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Purpose: </strong>To compare the effect of glass fiber post core porcelain crown and cobalt alloy post core porcelain crown in repairing residual root and crown of the anterior teeth.</p><p><strong>Methods: </strong>The data of 121 patients with residual root and crown of anterior teeth admitted to the hospital from January 2020 to December 2023 were reviewed, of which 61 patients were treated with glass fiber post-core porcelain crown restoration (experimental group) and 60 patients with cogger alloy post core all porcelain crown repair (control group). The repair outcomes of the two groups were recorded after 12 months of follow-up. The final results, aesthetic effect, periodontal health index, gingival crevicular fluid inflammatory factors and complications were compared between the two groups. SPSS 18.0 software package was used for statistical analysis of the data.</p><p><strong>Results: </strong>The success rate of the experimental group was significantly higher than that of the control group (P＜0.05). There was no significant difference in pink esthetic scores (PES) and white aesthetic scores (WES) between the two groups before and after treatment (P＜0.05). The sulcus bleeding index (SBI), gingival index (GI) and plaque index (PLI) after treatment were significantly lower in both groups than before treatment (P＜0.05), and the experimental group after treatment was more lower(P＜0.05). TNF-α, IL-6 and IL-8 levels after treatment were significantly lower in both groups than before treatment (P＜0.05), and TNF-α and IL-6 levels in the experimental group after treatment were more lower (P＜0.05). There was no significant difference in the total complication rate between the two groups (P＞0.05).</p><p><strong>Conclusions: </strong>Compared to cobalt alloy post-core all-porcelain crown, glass fiber post-core all-porcelain crown can enhance the repair effect, improve periodontal health, inhibit the damage of gingival crevicular fluid inflammation.</p>","PeriodicalId":21709,"journal":{"name":"上海口腔医学","volume":"34 2","pages":"166-170"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144476583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Evaluation of the efficacy of modified Twin-block appliance combined with micro-implant anchorage in the treatment of Angle Class Ⅱ division 1 malocclusion].","authors":"Chunyan Lu, Xuelei Cui, Peipei Cao","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Purpose: </strong>To explore the effectiveness of improved Twin block appliances combined with micro-implant anchorage in correction of Class Ⅱ division 1 malocclusion.</p><p><strong>Methods: </strong>A retrospective analysis of medical records of 96 patients with Class Ⅱ division 1 malocclusion treated at Rugao Hospital of Traditional Chinese Medicine from January 2018 to January 2022 was conducted. The patients were divided into experimental group (n=52) and control group (n=44) based on the treatment methods. The control group was treated with modified Twin-block appliance, while the experimental group was treated with modified Twin-block appliance combined with micro-implant anchorage. Comparison was made between the two groups regarding treatment duration, time to achieve bite opening, alignment time, ANB angle, SNB angle, SNA angle, U1-NA angle, U1-NB angle, U1-SN angle, L1-MP angle, upper lip protrusion(ULP), lower lip protrusion (LLP), Z angle, plaque index (PLI), gingival index (GI) and sulcus bleeding index (SBI). Complications and patient satisfaction were recorded. SPSS 24.0 software package was used for statistical analysis of the data.</p><p><strong>Results: </strong>The treatment duration, time to achieve bite opening and alignment time in the experimental group were significantly shorter than those in the control group (P＜0.05). After treatment, ANB, U1-NA and U1-SN of the two groups were lower than before treatment, and the experimental group was significantly lower than the control group (P＜0.05). U1-NB and L1-MP were higher than those before treatment, and the experimental group was significantly higher than the control group (P＜0.05). There was no significant difference between SNB and SNA (P＞0.05). After treatment, ULP and LLP of the two groups were lower than before treatment, and the experimental group was significantly lower than the control group(P＜0.05). Z-angle of the experimental group was significantly higher than that of the control group(P＜0.05). PLI in the experimental group was significantly lower than that in the control group (P＜0.05). There was no significant difference between GI and SBI (P＞0.05), and there was no significant difference in satisfaction between the two groups(P＞0.05).</p><p><strong>Conclusions: </strong>The modified Twin-block appliance combined with micro-implant anchorage can help to shorten the treatment time, improve the jaw and facial soft tissue contour, and promote the recovery of dental aesthetics.</p>","PeriodicalId":21709,"journal":{"name":"上海口腔医学","volume":"34 2","pages":"152-156"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144476605","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Segmentation and validation of mandibular canal and its bifurcation on cone beam CT based on deep learning].","authors":"Ye Ye, Shuobo Fang, Huitong Lu, Mingqian Liu, Xueying Wu","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Purpose: </strong>To train the U-net of convolutional neural network to establish a method for detecting and segmenting the mandibular canal and its bifurcation, and validate its accuracy based on the ground truth labeled by experts.</p><p><strong>Methods: </strong>A total of 290 CBCT scans were collected from Shanghai Stomatological Hospital from Jan. 2022 to Dec. 2022, which were divided into training set of 200 scans and test set of 90 scans. Model training included two steps. In the first step, bilateral mandibular canals and its bifurcation of 50 CBCT scans were labeled in 3D Slicer image computing platform by investigators. Three dimensional U-net segmentation model were trained initially with data enhancement. A morphological post-processing method was applied to the predicted results. In the second step, pseudo label method was employed to help annotating the mandibular canal and corresponding bifurcations on remaining 150 CBCTs, which would be included in training set after revision. Three dimensional U-net model was trained based on these 200 data. During test phase, totally 90 scans were labeled by two doctors and U-net model respectively. Consistency check was conducted to evaluate the labels between two doctors. Dice similarity coefficient and Hausdorff distance were calculated to evaluate the labels between doctors and the model. The detection rate of bifurcation was calculated. SPSS 20.0 software package was used for data analysis.</p><p><strong>Results: </strong>In 90 CBCT test set, the Kappa value between two dentists' annotations was 0.667. The average Dice and Hausdorff distance between predictions and labels of doctors were (0.739±0.068) and (0.988±1.14) mm. In bifurcation detection, the detection rate was 91.30% on scans with clear bifurcations.</p><p><strong>Conclsions: </strong>The dentification and segmentation U-net model of mandibular canal on dental CBCT can be reliable and practical for its high segmentation precision and predicting speed.</p>","PeriodicalId":21709,"journal":{"name":"上海口腔医学","volume":"34 2","pages":"119-125"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144476609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Effect of nonsurgical periodontal therapy on glycemic control in type 2 diabetes patients with chronic periodontitis].","authors":"Yu Shang, Yinhua Jiang, Yuanming Chen, Haibiao Fu, Fengdan Li","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Purpose: </strong>To investigate the effect of nonsurgical periodontal therapy on blood glucose control in patients with type 2 diabetes mellitus (T2DM) and chronic periodontitis, as well as the sensitive population.</p><p><strong>Methods: </strong>A total of 120 patients with T2DM and chronic periodontitis who were admitted to the Department of Endocrinology at Lishui City People's Hospital from March 2022 to March 2023 were randomly divided into experimental group and control group, with 60 patients in each group. All patients received oral health education, and their general information, community periodontal index (CPI), attachment loss (AL), fasting plasma glucose (FPG), and haemoglobin A1c (HbA1c) were collected. The experimental group received periodontal scaling and root planing. Three months later, CPI, AL, FPG, and HbA1c were measured. Stata 15.0 software was used for statistical analysis.</p><p><strong>Results: </strong>After periodontal scaling and root planing, the CPI, FPG, and HbA1c in the experimental group were significantly lower than those before treatment(P＜0.01) and those in the control group(P＜0.05). AL in the experimental group was also significantly lower than before treatment(P＜0.05) and the control group (P＜0.05). The change of FPG after treatment was positively correlated with the duration of diabetes (P＜0.01) and the baseline CPI(P＜0.05), but negatively correlated with whether suffering from hypertension(P＜0.05). It was also negatively correlated with the FPG level before treatment (P＜0.01). The change of HbA1c after treatment was positively correlated with the duration of diabetes(P＜0.05) and negatively correlated with the HbA1c level before treatment(P＜0.01).</p><p><strong>Conclusions: </strong>Nonsurgical periodontal therapy is helpful to blood sugar control of type 2 diabetes patients with periodontitis. Patients with higher baseline CPI levels and longer disease durations show greater improvement in glycemic control after treatment.</p>","PeriodicalId":21709,"journal":{"name":"上海口腔医学","volume":"34 2","pages":"202-207"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144476584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Expression of LINC 00478 in oral squamous cell carcinoma and its effect on proliferation, migration and invasion].","authors":"Qian Zhao, Linzi Han, Lu Zhao, Lili Wang, Likun Zhuang, Rongtao Yuan","doi":"","DOIUrl":"","url":null,"abstract":"<p><strong>Puepose: </strong>To examine the expression difference of LINC 00478 in oral squamous cell carcinoma (OSCC) and analyze its relationship with the prognosis of OSCC patients. To investigate the effects of LINC 00478 on proliferation, migration, and invasion of OSCC cells, with the goal of offering a new potential approach for molecular detection and clinical diagnosis of OSCC.</p><p><strong>Methods: </strong>Transcriptome data samples from OSCC were obtained from TCGA, and R software was utilized to assess the expression variance of LINC 00478 in OSCC tumor and normal tissues. Tissue samples from 26 OSCC patients, including cancer and adjacent tissues, were collected for RT-PCR analysis to determine the differential expression of LINC 00478 in OSCC tissues. In vitro experiments were conducted to explore the impact of LINC 00478 on proliferation, invasion, and migration of CAL27 cells. CCK-8 assay was used to observe the proliferation capability of LINC 00478 in CAL27 cells, while Transwell experiment was employed to assess its effects on cell migration and invasion. GraphPad Prism 8.0.2 software was used for statistical analysis of the data.</p><p><strong>Results: </strong>Analysis of the TCGA database revealed that LINC 00478 was weakly expressed in OSCC tissues compared to adjacent normal tissues (P＜0.001), with high-expression LINC 00478 correlating with improved patient prognosis(P＜0.05). Furthermore, LINC 00478 was significantly under-expressed in 26 OSCC tissues (P＜0.001). Experimental data from CCK-8 assays indicated that upregulation of LINC 00478 suppressed CAL27 proliferation (P＜0.05). Additionally, Transwell experiments demonstrated that high-expression LINC 00478 significantly inhibited CAL27 migration and invasion (P＜0.05).</p><p><strong>Conclusions: </strong>LINC 00478 is significantly under-expressed in OSCC, and its high expression is positively correlated with a better prognosis. Over-expression of the LINC 00478 gene has been shown to inhibit the proliferation, migration, and invasion of CAL27 cells, suggesting a potential role in inhibiting cancer progression in OSCC.</p>","PeriodicalId":21709,"journal":{"name":"上海口腔医学","volume":"34 2","pages":"126-131"},"PeriodicalIF":0.0,"publicationDate":"2025-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144476606","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}