Seed Science and Technology最新文献_第10页

SNP genotyping of maize (Zea mays) hybrids and parental inbred lines for genetic purity testing using double digest restriction site-associated DNA sequencing 利用双酶切酶切位点相关DNA测序技术对玉米(Zea mays)杂交种和亲本自交系进行遗传纯度检测的SNP基因分型

IF 1.4 4区农林科学

Seed Science and Technology Pub Date : 2021-01-01 DOI: 10.15258/sst.2021.49.3.02

V. Satya Srii, N. Nethra, K. Umarani, H. Lohithaswa, Y. Shadakshari, S. Rajendra Prasad

{"title":"SNP genotyping of maize (Zea mays) hybrids and parental inbred lines for genetic purity testing using double digest restriction site-associated DNA sequencing","authors":"V. Satya Srii, N. Nethra, K. Umarani, H. Lohithaswa, Y. Shadakshari, S. Rajendra Prasad","doi":"10.15258/sst.2021.49.3.02","DOIUrl":"https://doi.org/10.15258/sst.2021.49.3.02","url":null,"abstract":"Maize (Zea mays) is the third major cereal crop in the Indian subcontinent, but the crop yields per hectare of Indian maize cultivars are less than half of the global average due to the impurity of seed lots supplied to farmers. In this study, we discovered high-quality Single Nucleotide Polymorphic markers (SNPs) in two widely cultivated maize hybrids and their parental inbreds. Paired-end double digest restriction site-associated DNA sequencing was used to discover SNPs and a total of 30,764,454 reads with a read length of 151 bp per sample were generated. Genotyping of SNPs for maize hybrids ‘MAH 14-5’ and ‘Hema’ revealed a total of 47,812 and 15,815 Genetic Purity Analysis markers, respectively, of which 44,388 and 12,391 were unique with 3,424 being common to both hybrids. Identified SNPs were used to develop primers for Kompetitive Allele-Specific PCR genotyping assays to determine the genetic purity of 10 seed lots and the results were found to correlate with Grow-out-Tests. Thus, the SNPs discovered in this study proved reliable to test the genetic purity of commercial seed lots. Advances in plant molecular breeding tools especially ddRADseq for SNP discovery offer new opportunities to genotype existing cultivars and accelerate the production of genetically pure seeds.","PeriodicalId":21662,"journal":{"name":"Seed Science and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67090075","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Morphometry of castor bean seeds from the Durango State’s arid zone, Mexico 墨西哥杜兰戈州干旱地区蓖麻豆种子的形态测定学

IF 1.4 4区农林科学

Seed Science and Technology Pub Date : 2021-01-01 DOI: 10.15258/sst.2021.49.3.06

Gabriel de Jesús Peña-Uribe, Oscar Valdivia-Martínez, A. López-Santos, R. Valdez-Cepeda

引用次数: 3

Germination of coastalplain honeycombhead (Balduina angustifolia) in response to photoperiod, temperature and gibberellic acid 沿海平原蜂房(Balduina angustifolia)萌发对光周期、温度和赤霉素酸的响应

IF 1.4 4区农林科学

Seed Science and Technology Pub Date : 2021-01-01 DOI: 10.15258/sst.2021.49.3.09

G. Campbell-Martínez, M. Thetford, S. Wilson, C. Steppe, H. Pérez, Debbie L. Miller

{"title":"Germination of coastalplain honeycombhead (Balduina angustifolia) in response to photoperiod, temperature and gibberellic acid","authors":"G. Campbell-Martínez, M. Thetford, S. Wilson, C. Steppe, H. Pérez, Debbie L. Miller","doi":"10.15258/sst.2021.49.3.09","DOIUrl":"https://doi.org/10.15258/sst.2021.49.3.09","url":null,"abstract":"Coastalplain honeycombhead (Balduina angustifolia) is underutilised in ecosystem restoration and pollinator conservation projects. Seeds collected from different populations throughout Florida, USA during 2007 and 2016-2018 were used in experiments that evaluated the effects of a 0 or 12-hour photoperiod, seasonal and standard temperatures, 0-5,000 ppm gibberellic acid (GA) or source population (northwest, central and south Florida) on seed germination. For central Florida seeds, germination was high (62-74%) in all seasonal temperatures except for summer (4%) and was not affected by photoperiod. Germination differed for two northwest populations and was higher in warm (35/25 and 30/20°C) temperatures compared with cool (25/15 and 20/10°C) temperatures (63-72% vs. 9-36%). In a one-year move-along experiment, germination was similar at all seasonal and constant temperatures for seeds from central, south-central and south Florida, while northwest Florida seeds had reduced germination under winter temperatures. Germination of northwest Florida seeds after exposure to GA increased for two of four and three of four populations for one year and five-day old seeds, respectively. There were different temperature requirements on a regional scale and different dormancy levels at a local scale for coastalplain honeycombhead.","PeriodicalId":21662,"journal":{"name":"Seed Science and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67090217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

IF 1.4 4区农林科学

Seed Science and Technology Pub Date : 2021-01-01 DOI: 10.15258/sst.2021.49.3.10

Siriguleng, Mingjiu Wang, Hengshan Yang

{"title":"cGA3-related differential proteomic analysis of Phalaris arundinacea seeds during dormancy and at its release","authors":"Siriguleng, Mingjiu Wang, Hengshan Yang","doi":"10.15258/sst.2021.49.3.10","DOIUrl":"https://doi.org/10.15258/sst.2021.49.3.10","url":null,"abstract":"Herbage dormancy is a protective mechanism against adverse conditions, including low and irregular emergence rates during seeding. Phalaris arundinacea is an efficient C3 plant, which can be used for feed, fuel, water and soil conservation, and as an indicator plant for water eutrophication. Previous studies have found that its seeds are dormant, significantly affecting its agricultural use. However, the phytohormone gibberellic acid (GA3) can significantly shorten the dormancy period. GA3-related proteins that influence seed dormancy in P. arundinacea were investigated with two-dimensional electrophoresis and mass spectrometry. Based on the green plant protein NCBI database, 30 proteins showed significantly increased expression after GA3 treatment. Of these, 16 were up-regulated and 14 down-regulated. Of these, 25 proteins were identified by MALDI-MS-TOF and 20 were functionally identified. The identified proteins were involved in sugar metabolism, protein metabolism, RNA synthesis, fat metabolism, signal transduction, transportation, cytoskeleton, redox reactions and biosynthetic pathways. We carried out a key analysis of four proteins identified based on the Poaceae pasture proteome. The results indicated that germination induced by GA3 may be related to increased levels of peroxidase promoting glucose 6-phosphate oxidation through the reversible redox reaction of hydroquinone and quinone, thereby promoting the pentose phosphate pathway and initiating the cessation of dormancy and the start germination.","PeriodicalId":21662,"journal":{"name":"Seed Science and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67090844","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Early counts of radicle emergence, counted manually and by image analysis, can reveal differences in the production of normal seedlings and the vigour of seed lots of cauliflower 通过人工和图像分析对花椰菜胚根萌发的早期计数，可以揭示正常幼苗的生产和种子活力的差异

IF 1.4 4区农林科学

Seed Science and Technology Pub Date : 2021-01-01 DOI: 10.15258/sst.2021.49.3.04

T. Shinohara, S. Ducournau, S. Matthews, M. Wagner, A. A. Powell

{"title":"Early counts of radicle emergence, counted manually and by image analysis, can reveal differences in the production of normal seedlings and the vigour of seed lots of cauliflower","authors":"T. Shinohara, S. Ducournau, S. Matthews, M. Wagner, A. A. Powell","doi":"10.15258/sst.2021.49.3.04","DOIUrl":"https://doi.org/10.15258/sst.2021.49.3.04","url":null,"abstract":"The ability of a single early count of radicle emergence (RE) to predict differences in the production of normal seedlings (NG) and seed vigour was examined in four seed lots from each of three varieties of cauliflower (Brassica oleracea var botrytis). Initial seed quality was assessed in germination and controlled deterioration (CD) vigour tests. RE (first appearance of the radicle) was counted manually after 48 hours in the germination test (RE 48h 20/30°C) and every two hours for 144 hours at 20°C using automated image analysis. Seed vigour was assessed in field and glasshouse trials. A large proportion of the variance in NG was explained by the RE count. The same RE counts at 20/30°C and 20°C predicted seed vigour in the field and glasshouse and revealed the same vigour differences as the validated CD test. Differences in vigour (field and glasshouse emergence) observed between varieties were related to the extent of previously sustained seed ageing revealed by the CD test, although genotypic differences in vigour could not be excluded. We propose that single counts of RE taken manually or by image analysis can be used to reveal differences in the NG and vigour of seed lots of cauliflower.","PeriodicalId":21662,"journal":{"name":"Seed Science and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67090092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Blend wheat AL-type hybrid and using SSRs to determine the purity of hybrid seeds 混合小麦al型杂交种，并利用ssr测定杂交种纯度

IF 1.4 4区农林科学

Seed Science and Technology Pub Date : 2021-01-01 DOI: 10.15258/sst.2021.49.3.08

Y. Nie, De-Wei Kong, Fenjuan Cui, W. Sang, P. Mu, Hongjun Xu, xiaoming tian

{"title":"Blend wheat AL-type hybrid and using SSRs to determine the purity of hybrid seeds","authors":"Y. Nie, De-Wei Kong, Fenjuan Cui, W. Sang, P. Mu, Hongjun Xu, xiaoming tian","doi":"10.15258/sst.2021.49.3.08","DOIUrl":"https://doi.org/10.15258/sst.2021.49.3.08","url":null,"abstract":"Heterosis is a promising approach to increase wheat yield from a limited planting area. In this study, a fine quality restorer line 99AR144-1 and three stable male sterile lines, AL18A, AL36A and AL20A, were assigned as male and female, respectively. Seeds of the wheat line 99AR144-1 and three male sterile lines were mixed according to different proportions and then planted at an experimental farm at the Xinjiang Academy of Agri-Reclamation Sciences from 2013 to 2016. When the mixed sowing ratios of combinations 2 (AL36A × 99AR144-1) and 3 (AL20A × 99AR144-1) were 6 to 8%, the seed production yields were higher than the control; the yield of hybrid seed production increased by 98.8 and 19.9%, respectively. This increase was attributed to a rise in the outcrossing seed setting rate. Further, this study used the Xbarc-8 SSR (simple sequence repeat) molecular marker to identify the purity of blend hybrid seeds and establish a regression equation for hybrid seed purity testing. The coefficient of the regression equation were 0.9878 and 0.9689 respectively, which shows that the purity of hybrids can be accurately predicted by using this equation. This method can quickly and accurately identify the seed purity in mixed seed production.","PeriodicalId":21662,"journal":{"name":"Seed Science and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67090203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Physiological and biochemical characteristics of cold stratification to overcome morphophysiological dormancy in Glehnia littoralis seed 海苔种子冷分层克服形态生理休眠的生理生化特性

IF 1.4 4区农林科学

Seed Science and Technology Pub Date : 2021-01-01 DOI: 10.15258/SST.2021.49.1.03

C. Shao, Guan-Yi Wang, Xinhua Ding, Chao Yang, M. Yan

{"title":"Physiological and biochemical characteristics of cold stratification to overcome morphophysiological dormancy in Glehnia littoralis seed","authors":"C. Shao, Guan-Yi Wang, Xinhua Ding, Chao Yang, M. Yan","doi":"10.15258/SST.2021.49.1.03","DOIUrl":"https://doi.org/10.15258/SST.2021.49.1.03","url":null,"abstract":"Cold stratification is commonly used for breaking morphophysiological dormancy in Glehnia littoralis seeds. However, the physiological and biochemical characteristics of dormancy release are still unclear. For our study, dormant G. littoralis seeds were subjected to cold stratification (4°C). Physiological and biochemical characteristics, including germination percentage, the activities of antioxidant enzymes and α-amylase, and soluble sugar, soluble protein, H2O2, malondialdehyde (MDA) and coumarin were determined in imbibed seeds after different stratification periods (0, 30, 60, 90 or 120 days). The morphophysiological dormancy in G. littoralis seeds was completely released by cold stratification for 120 days. The activities of catalase (CAT) and content of hydrogen peroxide (H2O2) and total soluble sugars were significantly increased, while coumarins was significantly decreased during dormancy release. Dormancy release was significantly positively correlated with CAT activity (r = 0.903) and accumulation of H2O2 (r = 0.985) and total soluble sugars (r = 0.952), and significantly negatively correlated with coumarins (r = −0.919). Thus, the increase in H2O2, soluble sugars level and CAT activity and decrease in coumarins during cold stratification might contribute to promoting dormancy release in G. littoralis seeds.","PeriodicalId":21662,"journal":{"name":"Seed Science and Technology","volume":"34 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67090306","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Effects of Fe-Zn-NA chelates priming on the vigour of aged hybrid rice seeds and the maintenance of priming benefits at different storage temperatures 不同贮藏温度下Fe-Zn-NA螯合物对杂交水稻种子活力的影响及激发效益的维持

IF 1.4 4区农林科学

Seed Science and Technology Pub Date : 2021-01-01 DOI: 10.15258/SST.2021.49.1.05

Cheng Lin, Shan-shan Pan, Wei-min Hu, Y. Guan, Jin Hu, Wenjian Song

{"title":"Effects of Fe-Zn-NA chelates priming on the vigour of aged hybrid rice seeds and the maintenance of priming benefits at different storage temperatures","authors":"Cheng Lin, Shan-shan Pan, Wei-min Hu, Y. Guan, Jin Hu, Wenjian Song","doi":"10.15258/SST.2021.49.1.05","DOIUrl":"https://doi.org/10.15258/SST.2021.49.1.05","url":null,"abstract":"The effects of seed priming with Fe-Zn-NA chelate (FeSO4+ZnSO4+niacinamide) and the longevity of the beneficial priming effects were investigated for aged seeds of hybrid rice cv. Lingyouhuazhan (LYHZ) and cv. Longxiangyou 130 (LXY130). As compared with untreated and hydroprimed seeds, Fe-Zn-NA chelate priming significantly enhanced germination energy (GE), germination percentage (GP), germination index (GI), vigour index (VI) and normal seedling rate (NSR), and increased seedling shoot height (SH) and seedling dry weight (DW) in both cultivars. After priming with Fe-Zn-NA chelates, the GP of LYHZ was 96.0%, which was 12.7 and 12.0% higher than hydroprimed and non-primed seeds, respectively. Similarly, the GP of LXY130 was 89.5%, significantly higher than the hydroprimed and non-primed seeds (by 11.5 and 10.0%, respectively). When stored at 25°C, the benefits of Fe-Zn-NA chelate priming on seed vigour and viability was maintained for three months; while after six months storage, the GP of LYHZ and LXY130 was only 48.0 and 34.0%, respectively, which was significantly lower than the non-primed seeds (84.0 and 71.1%). When stored at 15 or 5°C for six months, the germination potential of Fe-Zn-NA chelate-primed seeds was still high. The GP of LYHZ and LXY130 seeds was 94.4% and 77.8%, respectively, after storage at 15°C, and 97.8% and 85.6% after storage at 5°C. In conclusion, the Fe-Zn-NA chelate priming was an effective method to improve the vigour and viability of hybrid rice aged seeds; however, the duration of beneficial priming effects needs to be determined according to the storage temperature.","PeriodicalId":21662,"journal":{"name":"Seed Science and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67090394","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Seed dormancy and germination characteristics of Acer cinnamomifolium, a plant species endemic to China 中国特有植物肉桂槭的种子休眠和萌发特性

IF 1.4 4区农林科学

Seed Science and Technology Pub Date : 2021-01-01 DOI: 10.15258/sst.2021.49.3.01

Cuifang Tang, Yu-Chen Zhao, Linfeng Ou, Cai-Hong Zhong, Song-Quan Song

{"title":"Seed dormancy and germination characteristics of Acer cinnamomifolium, a plant species endemic to China","authors":"Cuifang Tang, Yu-Chen Zhao, Linfeng Ou, Cai-Hong Zhong, Song-Quan Song","doi":"10.15258/sst.2021.49.3.01","DOIUrl":"https://doi.org/10.15258/sst.2021.49.3.01","url":null,"abstract":"Seed dormancy is an adaptive response of plants to environmental changes during long-term evolution. It plays an important role in plant survival and propagation. Acer cinnamomifolium, a plant species endemic to China, is an excellent garden and street tree species. However, dormancy and germination characteristics of A. cinnamomifolium seeds are not currently known. In the present paper, the mature seeds were used as experimental materials and we investigated the effect of temperature including alternating temperature, light, after-ripening, stratification and phytohormone on seed dormancy release and germination. After being incubated for 30 days, 0 to 10% of the seeds germinated in constant temperature (10, 15, 20, 25 and 30°C), while in alternating temperature regimes (20/10, 25/15 and 30/20°C), seed germination was < 35%. Dormancy release and germination of seeds can be increased by after-ripening, cold stratification and phytohormone treatments. We concluded that A. cinnamomifolium seeds have a non-deep physiological dormancy. Cold stratification at 4°C for 30 days and incubation thereafter at 20°C were found to be optimal for dormancy release and high seed germination.","PeriodicalId":21662,"journal":{"name":"Seed Science and Technology","volume":"1 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67090067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Are variations in kernel-related morphometric and chemical parameters correlated with differences in Sitophilus oryzae attack in maize? 玉米籽粒相关形态计量学和化学参数的变化与米象虫攻击的差异有关吗?

IF 1.4 4区农林科学

Seed Science and Technology Pub Date : 2021-01-01 DOI: 10.15258/SST.2021.49.2.02

C. Lanzanova, Cristina Agape, G. Castorina, C. Balconi, M. Alfieri, D. Locatelli, G. Consonni, L. Limonta

{"title":"Are variations in kernel-related morphometric and chemical parameters correlated with differences in Sitophilus oryzae attack in maize?","authors":"C. Lanzanova, Cristina Agape, G. Castorina, C. Balconi, M. Alfieri, D. Locatelli, G. Consonni, L. Limonta","doi":"10.15258/SST.2021.49.2.02","DOIUrl":"https://doi.org/10.15258/SST.2021.49.2.02","url":null,"abstract":"Insect infestation during seed storage affects cereal productivity. Sitophilus oryzae, which is present in various climates, is one of the primary pests for maize (Zea mays ) seeds. In this study, kernels of a set of eight inbred lines of importance for both maize breeding and research activities were characterised for morphological and biochemical parameters as well as for their susceptibility to S. oryzae. S. oryzae was reared in laboratory-controlled conditions and the susceptibility index (SI, range: 0-11), which depends on both number of emerging adults and their median developmental period, was measured. Maize kernels of each of the eight maize lines were put in contact with less than 3-day old males and females of S. oryzae for 13 days. Infested kernels were checked daily for new adult emergence. Four maize lines were susceptible to S. oryzae attack (SI = 7.5 to 9.7) while the others appeared to be moderately resistant (SI = 4.6-6.9). Our results highlighted the occurrence of variability for insect susceptibility among different maize genotypes, however, variations in the kernel traits analysed do not allow us to make predictions about the response to insect attack.","PeriodicalId":21662,"journal":{"name":"Seed Science and Technology","volume":"143 1","pages":""},"PeriodicalIF":1.4,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67090522","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0