Blend wheat AL-type hybrid and using SSRs to determine the purity of hybrid seeds

IF 1.7 4区农林科学 Q2 AGRONOMY

Seed Science and Technology Pub Date : 2021-01-01 DOI:10.15258/sst.2021.49.3.08

Y. Nie, De-Wei Kong, Fenjuan Cui, W. Sang, P. Mu, Hongjun Xu, xiaoming tian

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引用次数: 0

Abstract

Heterosis is a promising approach to increase wheat yield from a limited planting area. In this study, a fine quality restorer line 99AR144-1 and three stable male sterile lines, AL18A, AL36A and AL20A, were assigned as male and female, respectively. Seeds of the wheat line 99AR144-1 and three male sterile lines were mixed according to different proportions and then planted at an experimental farm at the Xinjiang Academy of Agri-Reclamation Sciences from 2013 to 2016. When the mixed sowing ratios of combinations 2 (AL36A × 99AR144-1) and 3 (AL20A × 99AR144-1) were 6 to 8%, the seed production yields were higher than the control; the yield of hybrid seed production increased by 98.8 and 19.9%, respectively. This increase was attributed to a rise in the outcrossing seed setting rate. Further, this study used the Xbarc-8 SSR (simple sequence repeat) molecular marker to identify the purity of blend hybrid seeds and establish a regression equation for hybrid seed purity testing. The coefficient of the regression equation were 0.9878 and 0.9689 respectively, which shows that the purity of hybrids can be accurately predicted by using this equation. This method can quickly and accurately identify the seed purity in mixed seed production.

查看原文本刊更多论文

混合小麦al型杂交种，并利用ssr测定杂交种纯度

杂种优势是在有限的种植面积上提高小麦产量的一种很有前途的途径。本研究选用优质恢复系99AR144-1和3个稳定雄性不育系AL18A、AL36A和AL20A分别为雄性和雌性。2013 - 2016年，将小麦品系99AR144-1与3个雄性不育品系按不同比例混合后，在新疆农垦科学院实验农场种植。组合2 (AL36A × 99AR144-1)和组合3 (AL20A × 99AR144-1)混播比例为6% ~ 8%时，制种产量高于对照;杂交制种产量分别提高98.8%和19.9%。这一增长归因于异交结实率的上升。利用Xbarc-8 SSR (simple sequence repeat，简单序列重复)分子标记对杂交种子纯度进行鉴定，建立杂交种子纯度检测的回归方程。回归方程的系数分别为0.9878和0.9689，表明该方程可以准确预测杂种的纯度。该方法能快速准确地鉴别混合制种中种子的纯度。

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来源期刊

Seed Science and Technology 农林科学-农艺学

CiteScore

3.00

自引率

28.60%

发文量

审稿时长

>36 weeks

期刊介绍： Seed Science and Technology (SST) is an international journal featuring original papers and articles on seed quality and physiology related to seed production, harvest, processing, sampling, storage, genetic conservation, habitat regeneration, distribution and testing. A journal that meets the needs of researchers, advisers and all those involved in the improvement and technical control of seed quality. Published every April, August and December.