发布求助
文献互助 智能选刊 最新文献

Science in China. Series B, Chemistry, life sciences & earth sciences最新文献

筛选
英文 中文
连续谱动力学的理论及应用——II.大气环流中的连续谱和离散谱 连续谱动力学的理论及应用——II.大气环流中的连续谱和离散谱
Science in China. Series B, Chemistry, life sciences & earth sciences Pub Date : 1995-12-10 DOI: 10.1360/ZB1995-25-12-1329
任舒展, 曾庆存
{"title":"连续谱动力学的理论及应用——II.大气环流中的连续谱和离散谱","authors":"任舒展, 曾庆存","doi":"10.1360/ZB1995-25-12-1329","DOIUrl":"https://doi.org/10.1360/ZB1995-25-12-1329","url":null,"abstract":"利用文献[1]的知识,分解了1985年冬季1月大气环流中的连续谱和离散谱,并在此基础上给出了逐日大气环流形式图,结果表明,在大气环流的大槽大脊中,连续谱扰动占很大比重。也求出了定常波及逐日扰中连续谱和离散谱对应的动能和位能、角动量及热量传输。在这些传输过程中,离散谱扰动在低层大气环流中有一定的作用,而对整个大气环流而言,连续谱扰动的确占据着主导地位。在很大程度上证实了曾庆存关于“连续谱扰动滋养西风”的结论在斜压大气环流中的正确性。","PeriodicalId":21648,"journal":{"name":"Science in China. Series B, Chemistry, life sciences & earth sciences","volume":"18 1","pages":"1329-1338"},"PeriodicalIF":0.0,"publicationDate":"1995-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78629354","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
1980～1993年我国“地热涡”的若干统计特征——II.“地热涡”与地震的统计相关 1980～1993年我国“地热涡”的若干统计特征——II.“地热涡”与地震的统计相关
Science in China. Series B, Chemistry, life sciences & earth sciences Pub Date : 1995-12-10 DOI: 10.1360/ZB1995-25-12-1313
汤懋苍 高晓清, Gao Xiao-Qing Shang Mao-Cang
{"title":"1980～1993年我国“地热涡”的若干统计特征——II.“地热涡”与地震的统计相关","authors":"汤懋苍 高晓清, Gao Xiao-Qing Shang Mao-Cang","doi":"10.1360/ZB1995-25-12-1313","DOIUrl":"https://doi.org/10.1360/ZB1995-25-12-1313","url":null,"abstract":"利用文献[1]的结果,进一步分析了“地热涡”与地震的关系。结果表明:凡水平尺度愈大或持续时间愈长的地热涡,发生的地震亦强,凡是“弱地热涡”,即使出现地震其震级亦弱;14a来我国强震(东部>5.5级,西部>6.5级)集中出现在地热涡半径比 r/R =0.32～0.61的范围内,另在半径比为0.73～0.79之间有一强震集中区, r/R >0.80之后,无强震出现;凡处在地壳垂直形变的上升中心区的地热涡,其地震方位角均在北或东方,只有贵州地热涡例外;凡处在地壳下降中心的地热涡,它们的地震出现在涡中心的东南～西方,只有汾渭河谷例外;发震时间与地热涡开始时间( T 3.2′ 开始≥0.5℃的时间)之差值与地震震级呈正相关。在文献[1]与本文事实分析的基础上,作者初步提出了一个地气耦合的概念模式图。","PeriodicalId":21648,"journal":{"name":"Science in China. Series B, Chemistry, life sciences & earth sciences","volume":"61 1","pages":"1313-1319"},"PeriodicalIF":0.0,"publicationDate":"1995-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74069497","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
含Yunnanese(Aγδβ)°-缺失的染色体11中Gγ珠蛋白基因在MEL细胞中活跃表达 含Yunnanese(Aγδβ)°-缺失的染色体11中Gγ珠蛋白基因在MEL细胞中活跃表达
Science in China. Series B, Chemistry, life sciences & earth sciences Pub Date : 1995-12-10 DOI: 10.1360/ZB1995-25-12-1285
张俊武 乔军 宋文风 邱志明, Jiao Jun Song Wen-Feng Qiu Zhi-Meng Zhang Dun-Wu
{"title":"含Yunnanese(Aγδβ)°-缺失的染色体11中Gγ珠蛋白基因在MEL细胞中活跃表达","authors":"张俊武 乔军 宋文风 邱志明, Jiao Jun Song Wen-Feng Qiu Zhi-Meng Zhang Dun-Wu","doi":"10.1360/ZB1995-25-12-1285","DOIUrl":"https://doi.org/10.1360/ZB1995-25-12-1285","url":null,"abstract":"用EB病毒转化技术获得了Yunnanese(Aγδβ)°-地中海贫血杂合子的淋巴细胞系,并用细胞融合法获得了其与鼠红白血病细胞(MEL)的杂合细胞,进一步筛选到仅含正常或异常人染色体11的次级克隆杂合细胞,并证明在只含正常人染色体11的次级克隆杂合细胞中Gγ,Aγ珠蛋白基因不表达,β珠蛋白基因表达,但在仅含具有缺失的人染色体11的杂合细胞中Gγ基因高水平表达。这些结果进一步确证在β-珠蛋白基因簇内的DNA片段缺失是导致Yunnanese(Aγδβ)°-地中海贫血中Gγ基因持续活跃表达的原因。","PeriodicalId":21648,"journal":{"name":"Science in China. Series B, Chemistry, life sciences & earth sciences","volume":"44 1","pages":"1285-1290"},"PeriodicalIF":0.0,"publicationDate":"1995-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76721095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
离子束辐照M13mp18 DNA(RF1)诱导lacZ突变的序列分析 离子束辐照M13mp18 DNA(RF1)诱导lacZ突变的序列分析
Science in China. Series B, Chemistry, life sciences & earth sciences Pub Date : 1995-12-10 DOI: 10.1360/ZB1995-25-12-1273
杨剑波, 吴李君, 李莉, 吴家道, 余增亮, 许智宏
{"title":"离子束辐照M13mp18 DNA(RF1)诱导lacZ突变的序列分析","authors":"杨剑波, 吴李君, 李莉, 吴家道, 余增亮, 许智宏","doi":"10.1360/ZB1995-25-12-1273","DOIUrl":"https://doi.org/10.1360/ZB1995-25-12-1273","url":null,"abstract":"用离子束辐照的M13mp18 DNA(RF1)转染宿主菌E.coli JM103发现:被处理的DNA除了一部分可以失去转染活性外,还能使其上的lacZ基因发生较高频率的突变,在存活率为20％左右时,lacZ的突变频率可达(3.6～16.8)×10 -4 ,为自发突变的2.3～10倍。通过对其中的10个突变体进行DNA测序,发现一些突变体在被测序的250bp范围内,可发生多达5～6个位点的碱基变异,这是在其它DNA直接辐照诱变的实验中(如X射线,γ射线,紫外线等)所很少见到的。碱基变异的类型则主要有转换(50％)、颠换(45％)和缺失(5％),转换主要以C→T和A→G为主,颠换则主要以C→A和C→G为主。在组成DNA的4个碱基中,尤以胞嘧啶最易发生变异(占整个突变碱基的60％)。通过对突变碱基周边序列进行比较发现,左右边界分别为TG和CT的碱基位点似乎比较容易发生碱基的变异。","PeriodicalId":21648,"journal":{"name":"Science in China. Series B, Chemistry, life sciences & earth sciences","volume":"57 1","pages":"1273-1278"},"PeriodicalIF":0.0,"publicationDate":"1995-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77217228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Identification of 2-[125I] iodomelatonin binding sites in the thymus of mice and its significance. 小鼠胸腺2-[125I]碘褪黑素结合位点的鉴定及其意义。
Science in China. Series B, Chemistry, life sciences & earth sciences Pub Date : 1995-12-01
Z Liu, Y Zhao, S Peng
{"title":"Identification of 2-[125I] iodomelatonin binding sites in the thymus of mice and its significance.","authors":"Z Liu,&nbsp;Y Zhao,&nbsp;S Peng","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The melatonin binding sites in membrane preparations of the mouse thymus were demonstrated using 2-[125I] iodomelatonin as a radioligand. The binding sites were stable, saturable, reversible and of high affinity. Studies on specificity of 2-[125I] iodomelatonin binding suggested that the 2-[125I] iodomelatonin binding sites are highly specific for melatonin. These binding sites fulfilled the standard criteria for receptors. Our work suggested that melatonin should have direct regulatory action on immune system mediated through the melatonin binding sites. Studies on the circadian rhythm showed that there existed the circadian rhythm in the binding capacity for 2-[125I] iodomelatonin in the mouse thymus with the peak values at 12:00-16:00 and the trough values between 00:00 and 4:00. The subcellular distribution of 2-[125I] iodomelatonin binding sites in the mouse thymus was in the following descending order: nuclear > mitochondrial > microsomal > cytosolic fraction. There was also an age-related decrease in 2-[125I] iodomelatonin binding in the mouse thymus. This is correlated with the involution of the thymus.</p>","PeriodicalId":21648,"journal":{"name":"Science in China. Series B, Chemistry, life sciences & earth sciences","volume":"38 12","pages":"1455-61"},"PeriodicalIF":0.0,"publicationDate":"1995-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19718345","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Relationship between the induction of heat shock proteins and the decrease in glucocorticoid receptor during heat shock response in human osteosarcoma cells. 人骨肉瘤细胞热休克反应中热休克蛋白诱导与糖皮质激素受体减少的关系。
Science in China. Series B, Chemistry, life sciences & earth sciences Pub Date : 1995-12-01
L Song
{"title":"Relationship between the induction of heat shock proteins and the decrease in glucocorticoid receptor during heat shock response in human osteosarcoma cells.","authors":"L Song","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Previously, it has been found that glucocorticoid receptor (GR) binding activity decreased rapidly during heat shock response in HOS-8603, a human osteosarcoma cell line. In this study, The relationship between the induction of heat shock proteins (HSPs) and the decrease in GR was further studied in the same cell line. It was found that even though quercetin could specifically inhibit the expression of hsp90 alpha and hsp70 mRNA, it could not prevent GR from the decrease in response to the heat shock treatment. This represents the first reported evidence that the induction of HSPs and the decrease in GR during heat shock response were 2 independent biological events. The results of the present study further showed that although the heat shock treatment alone had no effects on alkaline phosphatase (AKP) activity, it could completely block the induction of AKP activity in HOS-8603 cells by dexamethasone (Dex), a synthetic glucocorticoid. These results demonstrate that the heat shock-induced alteration in GR was accompanied by a decrease in GR functional activity. Furthermore, when the induction of HSPs was inhibited by the treatment of cells with quercetin, the stimulatory effects of Dex on AKP activity could still be inhibited completely by the heat shock treatment. The results of this part, on the basis of GR functional activity, further demonstrate that quercetin could not inhibit the heat shock-induced decrease in GR even though it could inhibit the induction of HSPs. To clarify further the effects of quercetin alone on GR binding activity in HOS-8603 cells, the regulation of GR by quercetin was also studied. It was found for the first time that quercetin could down-regulate GR in a time-dependent manner significantly, and that the down-regulation of GR by quercetin in HOS-8603 cells paralelled with a decrease in glucocorticoid-mediated functional responses, suggesting that the down-regulation of GR by quercetin is of biological significance.</p>","PeriodicalId":21648,"journal":{"name":"Science in China. Series B, Chemistry, life sciences & earth sciences","volume":"38 12","pages":"1473-81"},"PeriodicalIF":0.0,"publicationDate":"1995-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19718347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
1D and 2D 1H NMR studies on bisantrene complexes with short DNA oligomers. 双蒽配合物与短DNA低聚物的1D和2D 1H NMR研究。
Science in China. Series B, Chemistry, life sciences & earth sciences Pub Date : 1995-12-01
S Yao, W D Wilson
{"title":"1D and 2D 1H NMR studies on bisantrene complexes with short DNA oligomers.","authors":"S Yao,&nbsp;W D Wilson","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The binding of bisantrene to four DNA tetramers, d(CGCG)2, d(GCGC)2, d(CATG)2, and d(GTAC)2, was investigated by 1D and 2D NMR spectroscopy. Bisantrene is a well known anticancer drug and has been used clinically for years. DNA is believed to be one of its cellular targets. Results from both 1D and 2D 1H NMR are in agreement with an intercalation binding mode of bisantrene with the four DNA tetramers in this study. The results further indicate that a threading intercalation binding mode, in which one bisantrene side chain is in the minor groove and the other in the major groove of DNA, is preferred. The NMR results also suggest that bisantrene prefers binding at pyrimidine-(3',5')-purine intercalation sequences rather than at purine-(3',5')-pyrimidine sequences. The intramolecular and intermolecular NOE contacts of bisantrene-DNA tetramer complexes indicate that a C2'-endo uniform sugar pucker, rather than a mixed sugar conformation, is preferred by the intercalation site of both the 5'-(TA)-3' and the 5'-(CG)-3' binding steps.</p>","PeriodicalId":21648,"journal":{"name":"Science in China. Series B, Chemistry, life sciences & earth sciences","volume":"38 12","pages":"1462-72"},"PeriodicalIF":0.0,"publicationDate":"1995-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19718346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
斜压大气中连续谱动力学理论及运用——I.连续谱动力学理论 斜压大气中连续谱动力学理论及运用——I.连续谱动力学理论
Science in China. Series B, Chemistry, life sciences & earth sciences Pub Date : 1995-11-10 DOI: 10.1360/ZB1995-25-11-1210
曾庆存, 任舒展
{"title":"斜压大气中连续谱动力学理论及运用——I.连续谱动力学理论","authors":"曾庆存, 任舒展","doi":"10.1360/ZB1995-25-11-1210","DOIUrl":"https://doi.org/10.1360/ZB1995-25-11-1210","url":null,"abstract":"讨论球面准地转分层模型中连续谱动力学的理论框架,包括:从数学上严格地指出了支配方程中存在连续谱和离散谱两类性质不同的解;给出了连续谱和离散谱的分布范围;得到关于离散谱的“广义正交性原理”。并利用支配方程中存在的位涡拟能及准动量守恒性,给出了连续谱能量增长上限的估计式。对连续谱空间结构的演化作了定性的讨论,并给出扰动槽脊演化的几何图象。","PeriodicalId":21648,"journal":{"name":"Science in China. Series B, Chemistry, life sciences & earth sciences","volume":"113 1","pages":"1210-1218"},"PeriodicalIF":0.0,"publicationDate":"1995-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"76081686","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
端基附壁高分子链的构象统计理论——I. NRW尾形链 端基附壁高分子链的构象统计理论——I. NRW尾形链
Science in China. Series B, Chemistry, life sciences & earth sciences Pub Date : 1995-11-10 DOI: 10.1360/ZB1995-25-11-1121
吴大诚, 康健
{"title":"端基附壁高分子链的构象统计理论——I. NRW尾形链","authors":"吴大诚, 康健","doi":"10.1360/ZB1995-25-11-1121","DOIUrl":"https://doi.org/10.1360/ZB1995-25-11-1121","url":null,"abstract":"高分子在固体表面上吸附最简单的情况是仅有一个链端被吸附,即形成“尾形链”的构象。将此问题简化为半无限空间中的的随机行走问题,加以系统理论研究,导出了不同维数下尾形链的构象分布函数的公式,发现链长 N →∞时,尾形链构象数与自由链构象数之比随 N -1/2 而变化。并证明在受限边界的法向上均方末端距的分量增大一倍,而其余方向上的分量仍保持不变。","PeriodicalId":21648,"journal":{"name":"Science in China. Series B, Chemistry, life sciences & earth sciences","volume":"1 1","pages":"1121-1128"},"PeriodicalIF":0.0,"publicationDate":"1995-11-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72714555","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Construction, expression and characterization of tissue-type plasminogen activator mutants. 组织型纤溶酶原激活物突变体的构建、表达与表征。
Science in China. Series B, Chemistry, life sciences & earth sciences Pub Date : 1995-11-01
S Liu, P Huang, C Huang
{"title":"Construction, expression and characterization of tissue-type plasminogen activator mutants.","authors":"S Liu,&nbsp;P Huang,&nbsp;C Huang","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Three tissue-type plasminogen activator (t-PA) mutants were constructed by recombinant and site-directed mutagenesis techniques. They are del(296-302) with deletion of PAI-1 binding site, N117Q/N184Q with deglycosylation of K1 and K2 domains, and their combination mutant designated as GGI. Then these three mutants were successfully transiently expressed in COS-7 cells, and GGI was further stably expressed in CHO cells. The biological characterization of the expression products indicated that del(296-302) and GGI possessed the resistance to inhibition by PAI-1. In addition, the specific activity of GGI was increased by about 46%, the plasma half-life was prolonged by about one fold, while its affinity for fibrin was not affected.</p>","PeriodicalId":21648,"journal":{"name":"Science in China. Series B, Chemistry, life sciences & earth sciences","volume":"38 11","pages":"1341-8"},"PeriodicalIF":0.0,"publicationDate":"1995-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"19719004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
下一页 尾页
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整，为了账户安全，请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
请完成安全验证×
相关产品
×
本文献相关产品
联系我们：info@booksci.cn Book学术提供免费学术资源搜索服务，方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号：481959085
Book学术官方微信